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{
"caption": "The “intrinsic” model.\nA: Results of the “intrinsic” model simulations for large values for pBtoA. Note corresponding increase in proportion of type A cells (in red) and their random distribution. B: Analysis of type A (left panel) and type B (right panel) cell distributions in the “intrinsic” model as a function of average migration velocity using the standardized nearest neighbour distance (w). If w = 1, the cells are randomly distributed. Small standardized nearest neighbour distances (w<1) indicate clustering; this is only observed for B cells with very low average migration velocities (<0.2). In these examples pAtoB = 0.7 and pBtoA = 0.02, but similar results were obtained for other values of p.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851097-3-ponep0000394pg002.jpg"
}
|
001300
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Clonal population derived from a human primary myoblast.\nA: The cells were fixed and immunostained with an anti-desmin antibody. A series of 55 pictures were obtained and compiled into a single picture showing the whole population. The variation in the intensity of desmin immunostain is higher at the periphery of the growing population. The left panel shows 4 high resolution images of different parts of the population pointed by the blue arrows. B: Colour coded image of the same population as on the A panel. The colour code is based on the intensity of the pixels (red: low, green: high intensity). On the left are shown 2 high resolution images with the same color code. In the upper left, a region of interest is indicated in white with the corresponding pixel histogram shown beneath. Two additional histograms show the pixel intensities of two regions of interest: high-(green) and a low-desmin expressing cells (red). Note that the low desmin expressing cells are more frequent at the periphery of the culture.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851097-6-ponep0000394pg007.jpg"
}
|
001301
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "T-oligos localize to nuclei. MCF-7 and normal mammary epithelial (NME) cells were supplemented with fluorescein phosphoramidite (FAM) labeled GTTAGGGTTAG and uptake was determined 30 to 60 minutes after supplementation. Multicolor fluorescent microscopy showed that FAM-labeled T-oligos accumulated in the nucleus as determined by colocalization of propidium iodide (PI) nuclear staining (red) with FAM (green), producing an orange color. As expected, no green fluorescence was observed in control, diluent-treated cells.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851376-1-bcr1646-1.jpg"
}
|
001302
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "PMA stimulation prior to urokinase plasminogen activator (uPA) incubation mediates increased plasminogen (plg) binding at the MCF-7 cell surface. Confocal microscopy images of control and PMA-stimulated (100 nM, 16 hours) MCF-7 cells both incubated with 50 nM uPA for 10 minutes at room temperature, washed, and probed for annexin II (AnnII), uPA, and Glu-plg binding. Areas of co-localisation of annexin II, uPA, and Glu-plg are shown in white in the merged image. Arrows indicate areas of concentrated expression. Inset shows non-lysine-dependent plg binding (that is, plg binding in the presence of 5 mM tranexamic acid). Glu, glutamic acid; PAS, plasminogen activation system; PMA, 12-O-tetradecanoylphorbol 13-acetate.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851380-1-bcr1647-4.jpg"
}
|
001303
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Plasminogen activation system component changes on PMA-treated MCF-7 cells. MCF-7 cells were cultured for 16 hours in 5% foetal calf serum/RPMI containing 100 nM PMA. (a) Cells were washed and incubated for 30 minutes on ice with 10 to 20 μg/ml of anti-uPAR monoclonal antibody (mAb), anti-uPA mAb, anti-tPA mAb, anti-annexin II (Ann II) C-16 polyclonal antibody (pAb), anti-S100A10 (p11) pAb, or a matched isotype control antibody. Cells were then washed and incubated for 45 minutes on ice in the dark with a 1:200 dilution of appropriate secondary antibody labelled with fluorescein isothiocyanate (FITC). All cells were then washed and analysed for cell-surface-associated FITC fluorescence in the presence of 5 μg/ml propidium iodide using dual-colour flow cytometry. Specific antibody binding was calculated by subtracting control antibody fluorescence. Solid line indicates a fold increase of 1 (equivalent to no change). P values indicate a significant increase compared to the corresponding untreated control. (b) Confocal microscopy on attached cells was also used to verify enhancement of cell-surface uPAR by PMA treatment. PMA, 12-O-tetradecanoylphorbol 13-acetate; tPA, tissue-type plasminogen activator; uPA, urokinase plasminogen activator; uPAR, urokinase plasminogen activator receptor.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851380-3-bcr1647-2.jpg"
}
|
001304
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Effect of fibroblasts and conditioned media on E-cadherin and β-catenin (co)localization in PMC42-LA organoids. (a) Using immunocytochemistry and confocal microscopy, localization of E-cadherin was analyzed. Nuclei were visualized using ethidium bromide (red). (Part A) In control PMC42-LA organoids (no fibroblasts or conditioned media), E-cadherin staining was observed at cell junctions, which was confirmed by confocal microscopy sectioning of PMC42-LA in 2-dimensional culture (right panel). (Part B) In NMF-conditioned medium E-cadherin label was detected as both junctional and cytoplasmic in PMC42-LA organoids, as confirmed by confocal microscopy sectioning of PMC42-LA in 2-dimensional culture containing NMF-conditioned media (right panel). (Part C) In CAF-conditioned media E-cadherin was also detected as junctional and cytoplasmic in PMC42-LA organoids, with more predominant cytoplasmic localization. This was confirmed in 2-dimensional PMC42-LA culture containing CAF-conditioned media by confocal microscopy sectioning (right panel). With (part D) NMFs beneath filter or (part E) CAFs beneath the filter, E-cadherin was again detected at cell junctions and within cytoplasm. (b) E-cadherin and β-catenin are indicated by green and red label, respectively. Areas of colocalization appear yellow. (Part A) In control PMC42-LA cells (no fibroblasts or conditioned media), E-cadherin and β-catenin colocalized at cell junctions with some areas of non-colocalization. (Part B) When in NMF-conditioned medium, colocalization was detected at cell junctions with some independent localization. (Part C) In CAF-conditioned medium, E-cadherin localized to cytoplasm and β-catenin to cytoplasm and nuclei, with some overlap. CAF, cancer-associated fibroblast; NMF, normal mammary fibroblast.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851381-3-bcr1656-3.jpg"
}
|
001305
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "The effect of fibroblast-conditioned media on PMC42-LA organoid morphology in three-dimensional cultures. CAF-conditioned medium was added to or below PMC42-LA filter cultures, and the cultures analysed for changes in organoid morphology possibly representative of increased invasiveness. Controls had no fibroblast conditioned-medium. (a,b) In control cultures, organoids appeared spherical with little, if any, single cells present. (c,d) With CAF-conditioned media on the filter/culture, organoids remained predominantly spherical, with some budding edges and the presence of single cells and clusters of single cells. (e,f) With CAF-conditioned medium below the filter/culture, organoids appeared less spherical, with uneven budding edges and many single cells and clusters of single cells. CAF, cancer-associated fibroblast.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851381-4-bcr1656-7.jpg"
}
|
001306
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Lactation failure in SirT1ko/ko mice. (a) Whole mount analysis of the mammary tissues from virgin wild-type (+/+) and SirT1ko/ko mice (ko/ko). Scale bar = 2 mm. (b) Histological analysis of the mammary tissues harvested from wild-type (+/+) and SirT1ko/ko female mice on either day 13 of pregnancy (P13) or lactation day 1 (L1). Scale bars = 100 μm. (c) Immunofluorescence staining of milk production in the mammary tissues from wild-type (+/+) and SirT1ko/ko mice on L1. Red and smear stains, mouse milk proteins; blue dots, nuclear staining of adipocytes, epithelial cells, and other cells in mammary tissues. Scale bars = 100 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851382-0-bcr1632-2.jpg"
}
|
001307
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Mammary epithelial cell proliferation and apoptosis in SirT1ko/ko mice on lactation day 1. (a) Upper panel: bromodeoxyurdine (BrdU) staining of the mammary sections from SirT1ko/ko (ko/ko) and wild-type (+/+) mice on day 13 of pregnancy (P13) and lactation day 1 (L1). From left to right, three terminal end buds (TEBs) at a scale of 100 μm, a single TEB, ducts, and alveolus of SirT1ko/ko mice at a scale of 20 μm, and ducts and alveolus of wild-type mice on P13 and L1 at a scale of 20 μm. Lower panel: TUNEL (Terminal deoxynucleotidyl transferase mediated dUTP Nick End Labeling) assay for apoptotic cells using sections neighbored to the sections for BrdU staining. (b) The quantitative analysis of BrdU-positive epithelial cells in TEBs, ducts, and alveoli of SirT1ko/ko mice on L1 (open bar) and wild-type mice on P13 (grey bar) and L1 (solid bar). (c) The quantitative analysis of TUNEL-positive cells in neighboring sections.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851382-1-bcr1632-5.jpg"
}
|
001308
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Pregnancy-induced ductal morphogenesis. (a) Whole mount analysis of mammary gland development. Upper panel: wild-type (+/+) mice show terminal end buds (TEBs) at the onset of puberty, elongated ducts (virgin mice), site branching during pregnancy (P13), and lobuloalveolar structures for milk production on lactation day 1 (L1). Lower panel: virgin SirT1ko/ko (ko/ko) mice show impeded ductal morphogenesis. Pregnancy-induced ductal morphogenesis manifests transitional TEBs, ductal elongation, and side branching with variety in three SirT1ko/ko mice on L1. All scale bars = 200 μm. (b) Western blot analysis of both insulin-like growth factor-1 binding protein-1 (IGFBP-1) and IκBα in mammary tissues of male mice, virgin mice, and L1 female mice of wild type (+/+) and SirT1ko/ko (ko/ko). Actin is used as a loading control. Lower panel scores the estimated density of indicated lineages of cells in the protein extracts, which are based on the morphological analyses in (a).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851382-3-bcr1632-3.jpg"
}
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001309
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Immunolocalization of Sdc1, E-cad and c-met in human breast cancer cell lines. The human breast cancer cell lines MCF-7, MDA-MB468 and MDA-MB231 were stained with antibodies specific for c-met (green fluorescent secondary antibody) and Sdc1 or E-cad (red secondary antibodies), and observed by confocal immunofluorescence microscopy. Yellow staining denotes colocalization of c-met with Sdc1 or E-cad. E-cad, E-cadherin; Sdc, syndecan.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851383-0-bcr1641-1.jpg"
}
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001310
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Representative immunohistochemical staining patterns for E-cad, c-met, and Sdc1 in DCIS. Examples for presence (positive) and absence (negative) of marker expression are shown. DCIS, ductal carcinoma in situ; E-cad, E-cadherin; Sdc, syndecan.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851383-1-bcr1641-3.jpg"
}
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001311
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Breast tumours exhibit various levels of leucocyte infiltration. Normal breast and breast carcinoma were stained for B lymphocytes, T lymphocytes and macrophages using CD3, CD20 and CD68 immunostaining. Representative pictures of (a) normal breast, (b) tumour with weak leucocyte infiltration and (c) tumour with robust leucocyte infiltration are shown here.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851386-0-bcr1648-1.jpg"
}
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001312
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Histological appearance of basal tumours and CK14 expression patterns. (a) Typical histological appearance of a basal breast carcinoma (haematoxylin and eosin). (b) CK14 expression: a basal tumour with basal keratin expression in almost every tumour cell (diffuse staining pattern). (c) Another basal tumour with only a minority of tumour cells showing expression (focal staining pattern).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851397-0-bcr1636-1.jpg"
}
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001313
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Immunohistochemistry of bromodeoxyuridine-labeled mammary epithelial cells. The brown nuclei (arrows) represent the uptake of bromodeoxyuridine into cells undergoing DNA synthesis. (a) Untreated; (b) treated with estrogen plus progesterone.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851398-8-bcr1645-6.jpg"
}
|
001314
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Application of TREM2-Transduced Myeloid Cells Reduced Axonal and Myelin Injury(A) Reduced spinal cord axonal injury and decreased demyelination after cell therapy by TREM2-transduced myeloid cells. Axonal injury and damage were analyzed by immunostaining with specific antibodies directed against APP and dephosphorylated neurofilament (SMI32) at day 14 after cell application. Demyelination was determined by LFB staining at day 14 after cell application. Mice were injected at day 4 after first clinical signs of EAE by TREM2-transduced myeloid precursor cells (TREM2-BM-MC), GFP-transduced BM-MC (GFP-BM-MC), or PBS control. Representative histological pictures are shown. Scale bars for APP and LFB indicate 200 μm, and for inserts indicate 50 μm. Scale bar for SMI32 indicates 50 μm, and for insert indicates 20 μm.(B) Quantification of axonal damage and demyelination 14 d after cell application in EAE with TREM2-transduced myeloid cells (black bars), control GFP vector–transduced myeloid cells (green bars), or vehicle PBS control (red bars). Level of axonal injury (APP-positive deposits/mm2), axonal damage (relative number of SMI32-positive axons), and demyelination (loss of LFB, as a percentage) were significantly reduced after cell therapy with TREM2-transduced BM-MC. For quantitative analysis, at least three lumbar spinal cord sections of three mice per group were investigated. Data are presented as mean ± SEM. ANOVA followed by unpaired t-test: p = 0.0425 (TREM2 versus GFP) and p = 0.0759 (TREM2 versus PBS) in APP; p = 0.0012 (TREM2 versus GFP) and p = 0.0098 (TREM2 versus PBS) in SMI32; p = 0.0465 (TREM2 versus GFP) and p = 0.00450 (TREM2 versus PBS) in LFB.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851623-0-pmedp0040124pg007.jpg"
}
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001315
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Migration of TREM2-Transduced Myeloid Cells into EAE Lesions(A) Detection of invaded myeloid cells in the spinal cord of EAE-diseased mice. Histological sections were obtained at day 2 after intravenous injection of GFP-transduced cells in EAE-diseased mice. Myeloid cells were injected at day 4 after first clinical signs of EAE. Sections were labeled with CD45-specific antibodies. Injected myeloid cells expressed CD45 and accumulated in inflammatory lesions. Scale bar indicates 50 μm.(B) No Iba1 antigen was detected on invaded myeloid cells by double immunofluorescence labeling. GFP+ cells were analyzed in the spinal cord of EAE-diseased mice 4 d after intravenous injection of GFP-transduced myeloid cells. Myeloid cells were applied 4 d after first clinical signs of the disease. Invaded cells showed round appearance, reminiscent of activated macrophage/microglial cells. Scale bar indicates 10 μm.(C) Quantification of cells invaded into the spinal cord of EAE-diseased mice. TREM2-transduced BM-MC or GFP-transduced BM-MC were injected intravenously at day 4 after the first clinical signs of the disease appeared. The BM-MCs migrated into spinal cord lesions within 2 h after intravenous application and remained there for 2–4 d. No GFP+ cells were detected within the spinal cord at day 7 after intravenous application. Data are presented as mean ± SEM.(D) Flow cytometry analysis of TREM2 expression on TREM2-transduced and GFP-transduced myeloid cells obtained from the spinal cord of EAE-diseased mice at day 2 after application. Expression of TREM2 was detected on myeloid cells transduced with TREM2 (red tracing), but not on cells transduced with GFP (green tracing). Tracings shown are from cells gated for GFP. Control antibody isotype is shown as grey-filled tracing.(E) Flow cytometry analysis of CD45 expression on GFP-transduced myeloid cells before injection (green tracing) and on GFP+ cells invaded into the spinal cord (red tracing) at day 4 after injection. Engrafted myeloid cells showed reduced levels of CD45 expression, reminiscent of microglia. Tracings shown are from cells gated for GFP. Control antibody isotype is shown as grey-filled tracing.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851623-7-pmedp0040124pg004.jpg"
}
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001316
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "In vivo Efficacy of ARA-C in a Mouse Model of Ewing Sarcoma(A) A673-Luciferase-positive xenografts were established for 2–3 wk in NCr nude mice, with tumor burden monitored by bioluminescence imaging. Mice with logarithmically growing tumors were treated with intraperitoneal injection of ARA-C at 50 mg/kg daily for four doses or vehicle control. In both a pilot experiment A: and a larger study with cohorts of nine mice in each group B: control mice continued to have logarithmic tumor growth, necessitating sacrifice within 1 wk of starting treatments. By comparison, ARA-C-treated mice had stable disease or tumor regression with seven of nine animals showing regression.(B) Arrows indicate days of drug dosing, and the asterisk indicates p < 0.05 by Student's t-test (two-tailed assuming samples with equal variance). Error bars show the standard deviation across nine replicates. Bioluminescence was measured as photonic flux through standardized regions of interest (photons per second per region of interest), which did not include reflected light along the left flank (e.g., right animal in part [A] at day 17 of ARA-C treatment).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851624-0-pmedp0040122pg006.jpg"
}
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001317
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Section of the tree sample that was used for magnetic measurements and tree ring density. Blue numbers indicate a year (AD) when the tree ring was created. Blue dots (pinpricks) help orientation in respect to individual tree ring ages. Each one dot is the 10th year, two vertical dots are the 50th year, three vertical dots are 100th year, and four vertical dots are the 1000th year.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851700-2-1467-4866-8-2-1.jpg"
}
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001318
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Effect of HBV infection on tubules formation of EPCs. Matrigel was polymerized on 96-well plates, and 1 × 104 of EPCs were plated in Medium 199 containing either 10% normal control serum (mock-treated, A) or10% HBV-positive serum (HBV-treated, B), and incubated at 37°C for 72 hrs. The tubules formation was visualized by an inverted microscope. Magnification for panel A, × 400; for panel B, × 600.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851702-0-1743-422X-4-36-2.jpg"
}
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001319
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Detection of HBV viral particles in EPCs. HBV-treated (A, B, and C) or mock-treated EPCs (D) or HBV-treated HUVECs (E and F) were cultured in Medium 199 for 7 days and the cells were analyzed by transmission electron microscope. Arrows indicate the virus particles with a diameter of 80 nm in B and C, vesicle in E, and rough endoplasmic reticulum (RER) in F. Bars are 500 nm in A, E, and F; 200 nm in B; 100 nm in C; and 1 μm in D.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851702-1-1743-422X-4-36-5.jpg"
}
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001320
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Detection of HBV antigens in mouse lung and kidney tissues. Lung interstitium (A-D) in MI mice and renal glomerular vascular endothelium (E-H) in MI mice with acute renal ischemia were immunostained with anti-HBcAg (A, C, E and G) and anti-HBsAg (B, D, F and H) after 2 weeks of cell transplantation. Magnification × 800. Mice transplantated either with HBV alone (A, B, E and F) or with HBV-treated EPCs (C, D, G and H) were indicated. Results are representative of at least three independent experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851702-4-1743-422X-4-36-7.jpg"
}
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001321
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Detection of HBV antigens in mouse heart tissues. Two weeks after cell transplantation, hearts were harvested, immunostained with anti-HBcAg (A, C, and E) and anti-HBsAg (B and D). Myocardial interstitium (A-D) and intra-myocardial capillaries (E) in the peri-infarct area were shown. Hematoxylin and eosin (H&E) staining of heart tissue after 2 weeks MI-induction revealed a clear infract area (F). Magnification for panels B and D, × 800; for panels A, C and E, × 400; and for panel F, × 40. In addition, hearts were double immunostained with HBcAg (G and I) and human HLA-ABC (H and J) 3 weeks after cell transplantation as described in \"Materials and Methods\". Intra-myocardial capillaries in the peri-infarct area were shown. Magnification × 400. Mice transplantated either with mock-treated EPCs (A, B, G, and H) or with HBV-treated EPCs (C-E, I and J) were indicated. Results are representative of at least three independent experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851702-5-1743-422X-4-36-6.jpg"
}
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001322
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Characterization of EPC. Isolated EPCs were visualized by phase-contrast microscope (A 400 ×), and DiI-Ac-LDL uptake was analyzed by fluorescent microscope (B 100 ×). The cells were further analyzed by immunostaining with anti-CD31(C 200 ×) and vWF antibodies(D 200 ×), or by flow cytometry with anti-KDR (E) and CD34/CD133 antibodies (F).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851702-6-1743-422X-4-36-1.jpg"
}
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001323
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Multiphoton Laser Scanning Microscopy (MPLSM) was performed on hematoxylin and eosin sections in order to evaluate the organization and structure of the collagen matrix in more detail then allowed by conventional brightfield light microscopy. The longitudinal axis of the ligament is from the top to bottom of each image. Tissues from sham control animals have the characteristic crimp pattern associated with normal tissue (Sham). Scar tissue from ambulatory healing animals (Amb + Sal) revealed typical scar morphology with matrix disorganization while hindlimb unloaded animals (HU + Sal) showed good fiber aggregation but possessed abnormal scar formation with misaligned collagen fibers not directed along the longitudinal axis of the tissue creating voids and defects by not connecting. Assessment of collagen matrices from GH treated animals revealed no improvement in matrix organization in tissues from ambulatory (Amb + GH) or hindlimb unloaded (HU + GH) animals. Examination of collagen structure in animals treated with IGF-I supported data shown in Fig. 4 revealing greatly increased matrix density and considerably improved matrix alignment in tissues from ambulatory (Amb + IGF) and hindlimb unloaded (HU + IGF) animals. Animals treated with GH+IGF showed no significant improvement in ambulatory tissues (Amb + GH + IGF) but unloaded tissues (HU + GH + IGF) showed substantially increased matrix density and alignment.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851714-1-1472-6793-7-2-5.jpg"
}
|
001324
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Representative tissue sections taken from the midsubstance region of control normal tissue (Sham) and scar tissues (Saline, GH, IGF-I, GH+IGF-I). The longitudinal axis of the ligament is from the top left to bottom right of each image (200X). Tissues from sham control (Sham) animals have the characteristic crimp pattern and aligned fibroblasts associated with normal tissue. Examination of scar tissue from ambulatory healing animals (Amb + Sal) revealed typical scar morphology with matrix disorganization and hypercellularity. In contrast to ambulatory animals, hindlimb unloaded (HU + Sal) animals showed abnormal scar formation with pockets of cell clusters and misaligned collagen fibers creating defects and voids by not connecting. Examination of extracellular matrices from GH treated animals revealed no improvement in matrix organization in tissues from ambulatory (Amb + GH) or hindlimb unloaded (HU + GH) animals. However, animals treated with IGF-I showed extensively increased matrix density and substantially improved matrix alignment in tissues from ambulatory (Amb + IGF) and hindlimb unloaded (HU + IGF) animals; indicating a considerable improvement in the matrix structure in animals treated with IGF-I. Animals that were treated with both GH and IGF-I showed similar improvement to that seen with IGF-I in unloaded tissues (HU + GH + IGF) but showed no improvement in ambulatory tissues (Amb + GH + IGF).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851714-2-1472-6793-7-2-4.jpg"
}
|
001325
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Sample TMA image from the virtual microscope section on the ACSR Mid-region Internet site for viewing TMS core tissues by interested investigators.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851770-1-1750-9378-2-7-2.jpg"
}
|
001326
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Distal subtraction angiogram shows a distal peroneal pseudoaneurysm and a mid peroneal aneurysmal dilatation.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851946-0-1471-2482-7-4-1.jpg"
}
|
001327
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Post embolisation angiogram showing coils in-situ and no flow into the Pseudoaneurys.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851946-1-1471-2482-7-4-2.jpg"
}
|
001328
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "SB-431542 rapidly blocks transcription of Nodal target genes. lefty1 expression in embryos treated with DMSO (A-C) or SB-431542 (D-F) at 4.3 h (dome stage), and fixed after 15 minutes (A, D), 30 minutes (B, E) or 45 minutes (D, F).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-1-1471-213X-7-22-2.jpg"
}
|
001329
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Treatment at MBT blocks the response to receptors activated during the cleavage stages. Embryos injected at the 1–4 cell stage with 10pg β-galactosidase (A-D), TARAM-D (E-H), or sqt (I-L) mRNA, and treated at 2.75 h with DMSO (A, E, I, C, G, K) or SB-431542 (B, F, J, D, H, L). (E, G) TARAM-D induces ectopic body axes and gsc expression. (F, H) The effects of TARAM-D are suppressed by treatment with SB-431542. (I, K) sqt overexpression arrests epiboly and induces ubiquitous expression of gsc. (J, L) The response to ubiquitous Sqt is blocked by treatment with SB-431542. Images of live embryos at 30 h, anterior to the left (A, B, E, F, I, J). Animal pole views of fixed embryos at 5 h, dorsal to the right (C, D, G, H, K, L).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-3-1471-213X-7-22-3.jpg"
}
|
001330
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Nodal signals pattern the mesoderm during a three-hour time window. (A1-A8) Trunk somites form in embryos treated at 3.7 h (A1, 5), but flh is expressed in four ectodermal domains (A6). (B1-B8) Embryos treated at 4 h contain more somites (B5). flh is expressed at the midline and small amounts of notochord tissue are observed in live embryos (B1, arrow). pax2.1 expression is also observed (B7). At later time points, embryos have progressively more somites and more notochord (C1-E7). flh expression extends further up the midline and pax2.1 is expressed more strongly. Kupffer's vesicle forms in embryos treated 4.3 h (C3, arrowhead). shhb is not expressed in embryos treated before gastrulation (A8-E8). Images of live embryos at 24 h, anterior to the left (A1-E1; A2-E2) or 14hpf (A3-E3); dorsal views of fixed embryos at 10 h (A4-E4; A6-E6; A8-D8) or 14 h (A5-E5; A7-E7). Control embryos are depicted in Fig. 1J-S, which are from the same experiment.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-4-1471-213X-7-22-4.jpg"
}
|
001331
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Nodal signals pattern the ventrolateral mesoderm along the animal-vegetal axis in a time-dependent manner. Heart myocardial cell fates were examined in embryos treated with DMSO (A1-C1), or with SB-431542 at various time points (A2-C5). (A2-C2) Embryos treated at 4 h, express small amounts of amhc and cmlc2, but not vmhc (arrows). (A3-3) cmlc2, amhc and vmhc are bilaterally expressed in embryos treated at 4.3 h. (A4-C5) All heart markers are expressed at the midline in embryos treated at 4.7 h. Images are dorsal views at 24 h, anterior to the left.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-5-1471-213X-7-22-7.jpg"
}
|
001332
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Cell fate specification is delayed squint mutants. Cell fates were examined in sqt mutant embryos treated with DMSO (A1-D1), or with SB-431542 at various time points. (A1-7) flh was first expressed at the midline in embryos treated at 5 h (A5). (B2-7) gsc expression is first detected in embryos treated at 6 h (B6). (C2-7) sox17 expression is first detected when embryos are treated at 7 h (C7). (D1-7) cmlc2 expression was first detected in embryos treated 4.7 h (D4, arrowhead). Dorsal views of 10 h (A1-B7), 8 h (C1-C7) or 24 h (D1-D6). In D1-D6, anterior is up. The embryos in Figs. 8 and 9 are from the same clutch and weretreated in parallel, along with wild type controls (not shown).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-7-1471-213X-7-22-8.jpg"
}
|
001333
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Treatment with 800 μM SB-431542 or 50 μM SB-505124 at MBT prevents formation of mesoderm and endoderm. (A-F) Images of live embryos at 24hpf treated at 2.75 h with DMSO (A-C), SB-431542 (D-F; J-S), or SB-505124 (G-I). Embryos treated with SB-431542 (D-F) or SB-505124 (G-I) lack derivatives of the mesoderm and endoderm in the head and trunk, display severe cyclopia and lack Kupffer's vesicle. (J-P) Images of embryos treated with DMSO (J-N) or SB-431542 at MBT (O-S) and processed to reveal expression of markers for derivatives of dorsal mesoderm (ntl: J, O; flh: L, Q), paraxial mesoderm (myoD: K, P), intermediate mesoderm (pax2.1: M, R), and ventral neurectoderm (shhb: N, S). Dorsal views of embryos fixed at 10 h (J, L, N, P, Q, S)or 14 h (K, M, P, R). Arrowhead in (C) is the Kupffer's vesicle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_110-PMC1851950-8-1471-213X-7-22-1.jpg"
}
|
001334
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "MRI of the left thigh, sequence T1-weighted: a) precontrast image: \"ovular mass in the adductor magnus muscle, with a cystic aspect, dimensions 13 × 8 cm containing numerous cystic formations with regular outlines; b) after contrast image: \"the lesion appears surrounded by a thin wall homogeneously impregnated after the injection of paramagnetic contrast medium while there is no contrastographic enhancement of the content of the lesion\".",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851964-0-1471-2334-7-23-1.jpg"
}
|
001335
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Macroscopic aspect of the hydatid cyst after the surgical excision.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851964-1-1471-2334-7-23-2.jpg"
}
|
001336
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "A: Angio-embolization of the pseudoaneurysm with gelfoam and microcoil. B: Post embolization image showing non-filling of the pseudoaneurysm (arrow).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851966-0-1471-230X-7-12-2.jpg"
}
|
001337
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "A: Flush aortogram showing pseudoaneurysms in the coeliac artery territory. B: Selective hepatic artery angiogram showing two small pseudoaneurysms (arrow) in relation to the cystic artery.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851966-1-1471-230X-7-12-1.jpg"
}
|
001338
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Macroscopic appearance of duodenal GIST specimen.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851968-1-1477-7800-4-9-4.jpg"
}
|
001339
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Intra-operative view of the duodenal GIST.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851968-2-1477-7800-4-9-2.jpg"
}
|
001340
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "CT scan of patient showing duodenal GIST.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851968-3-1477-7800-4-9-1.jpg"
}
|
001341
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Chemotaxis and EPS Synthesis in H. arsenicoxydans in Response to Arsenic(A) Chemoattraction to As[III]. Left: low melting agar with chemotaxis buffer, right: low melting agar with chemotaxis buffer supplemented with 2 mM As[III]. Bright ring of cells around agarose plugs is indicative of chemotaxis.(B) Transmission electron microscopy (TEM) picture of H. arsenicoxydans grown in As-enriched medium. Circles represent the X-ray spot of analysis, while I and II are the energy dispersive X-Ray spectroscopy corresponding values. Cl and K peaks show organic constituents and Cu labels represent peaks due to supporting grid. Arsenic content is 16.5 % weight as As2O3 in I. and 0.0% weight in II; both including microgrid C-coating quantification.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851979-0-pgenp0030053pg007.jpg"
}
|
001342
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "IGs purified from the trophocytes of honeybees. (A) A large amount of IGs appear in precipitates purified from the trophocytes. Scale bar, 1 µm. (B) Purified IGs enclosed with lipid-bilayer membranes (arrowhead). Scale bar, 100 nm. (C) EDX spectrum is obtained from an IG in the purified precipitates. Iron (Fe), calcium (Ca) and phosphorus (P) are present in an IG. STEM mode of 100-kV accelerating voltage was used; counts were made for 100 seconds. (D) EDX spectrum of spurr's resin is devoid of IGs in the purified precipitates. The experimental conditions are the same as in C. (E) Size distribution of purified IGs calculated from the TEM pictures (N = 274). N, total number of granules used in this calculation.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851986-1-ponep0000395pg002.jpg"
}
|
001343
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "The images of increase of [Ca+2]i in the live trophocytes obtained by confocal microscope. (A) The image of trophocytes and fat cells under confocal microscope. Trophocytes (arrowhead). Fat cells (arrow). Scale bar, 40 µm. (B) The image of trophocytes and fat cells labeled fluo 4 under confocal microscope. Trophocytes (arrowhead). Fat cells (arrow). Scale bar, 40 µm. (C) The merge of Figure A and Figure B. Scale bar, 40 µm. (D) The fluorescence intensity of trophocytes (○), fat cells (Δ), and trophocytes and fat cells together (□) with 1 Gauss magnetic field. The fluorescence intensity of trophocytes (•), fat cells (▴) and trophocytes and fat cells together (▪) without 1 Gauss magnetic field as control. (E) The fluorescence intensity of trophocytes (○), fat cells (Δ), and trophocytes and fat cells together (□), which is inhibited by colchicine and with 1 Gauss magnetic field. The fluorescence intensity of trophocytes (•), fat cells (▴) and trophocytes and fat cells together (▪), which is inhibited by colchicine without 1 Gauss magnetic field as control. (F) The fluorescence intensity of trophocytes (○), fat cells (Δ), and trophocytes and fat cells together (□), which is inhibited by latrunculin B and with 1 Gauss magnetic field. The fluorescence intensity of trophocytes (•), fat cells (▴) and trophocytes and fat cells together (▪), which is inhibited by latrunculin B without 1 Gauss magnetic field as control.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851986-2-ponep0000395pg005.jpg"
}
|
001344
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "The images of MGs in the trophocytes obtained by confocal microscope. (A) MGs (arrowhead) appear as tiny black particles under low magnification in the live trophocytes. Arrow indicates oil body. Scale bar, 8 µm. (B) MGs (arrowhead) appear as black granules under high magnification in the live trophocytes. The images of MGs are obtained without the application of 1 Gauss magnetic field. Arrow indicates oil body. Inset shows a magnified MG. Scale bar, 1 µm. (C) The same images obtained by the application of 1 Gauss magnetic field in the live trophocytes. White arrow indicates the direction of magnetic field. Arrowhead indicates MGs. Arrow indicates oil body. Inset shows a magnified MG. Scale bar, 1 µm. (D) MGs (arrowhead) appear as tiny black particles under low magnification in the dead trophocytes. Scale bar, 8 µm. (E) MGs (arrowhead) appear as black granules under high magnification in the dead trophocytes. The images of MGs are obtained without the application of 1 Gauss magnetic field. Inset shows a magnified MG. Scale bar, 1 µm. (F) The same images obtained by the application of 1 Gauss magnetic field in the dead trophocytes. Arrowhead indicates MGs. White arrow indicates the direction of magnetic field. Inset shows a magnified MG. Scale bar, 1 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851986-3-ponep0000395pg004.jpg"
}
|
001345
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "The pattern of proliferation along the heart tube is dynamic and is regulated by Tbx3 and Tbx2.All images represent reconstructions of confocal Z-stack sections imaged on whole embryos at 31hpf (A) and 33 hpf (B–G). (A, B) During the first steps of looping, the pattern of proliferation shifts from homogenous throughout the heart tube (31 hpf, A) to a heterogenous one in which dividing cells are more concentrated in the future chambers (33 hpf, B). (C, D) In Tbx3- (C) and Tbx2- (D) injected embryos at 33 hpf this shift has not occurred and the number of dividing cells was significantly decreased and dividing cells were homogenously distributed (H). (E–G) MO-injected embryos against tbx3b (E), tbx2a (F) or both (G) display the same (E, F) or higher (G) number of proliferating cells than wild type at 33 hpf. However, proliferating cells remain homogenously distributed throughout the heart tube. (H) Histogram showing the average of the total number of BrdU positive cells in the heart of 33 hpf embryos: wt, 31.4±1.661 (n = 5); Tbx3 mRNA, 17.5±1.708 (p<0.001; n = 6); Tbx2 mRNA, 17.0±1.000 (p<0.001; n = 7); tbx3b MO, 28.0±0.408 (n = 4); tbx2a MO, 31.3±2.658 (n = 4); double MO, 36.8±1.797 (n = 4). a, atrium; h, heart; nt, neural tube; v, ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-0-ponep0000398pg004.jpg"
}
|
001346
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Heart morphology in embryos overexpressing Tbx3 and Tbx2.Side views (A–C) and ventral views (D–F) of wild type embryos (A, D) and embryos injected with 100 pg capped mRNA for mouse Tbx3 (B, E) or human TBX2 (C, F). (A, B, C) mlc2a::GFP embryos at 60 hpf; (D, E, F) 48 hpf. (B, C) 44.5% of Tbx3-injected embryos and 60% of Tbx2-injected embryos present a pipe-like heart that lacks chamber growth and looping. (D, E, F) Zebrafish bmp10 is strongly expressed in the chamber myocardium in wild type embryos, but is significantly downregulated from the heart in Tbx3- or Tbx2-injected embryos. a, atrium; v, ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-1-ponep0000398pg003.jpg"
}
|
001347
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "in situ hybridization analysis of cardiogenesis in the absence of tbx3b and tbx2a.Ventral views of the heart in its maximum extension from the anterior to the posterior pole. (A) bmp4 is expressed in the non-chamber myocardium of the heart, the OFT, the AVC and the IFT. (B, C, D) The expression pattern of bmp4 is normal in 42 hpf tbx3b, tbx2a and double morphants. (E) notch1b is expressed in the whole endocardium of the heart tube, but becomes restricted to the endocardium at the level of the OFT and AVC upon looping. (F, G, H) Expression of notch1b is maintained in the endocardium at the level of the chamber myocardium in 48 hpf tbx3b, tbx2a and double morphants, in contrast to wild type. (I) bmp10 is expressed exclusively in chamber myocardium at 42 hpf (J, L, M) In 42 hpf tbx3b and tbx2a morphants bmp10 is expressed in the chamber myocardium and is downregulated in the AVC, as is the case in wild type, but it is not downregulated from the AVC in double morphants. Red arrow indicates the AVC. a, atrium; h, heart; v, ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-2-ponep0000398pg002.jpg"
}
|
001348
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Mice with a targeted mutation for Tbx3, Tbx3neo display cardiac defects.(A, B) Severely affected Tbx3 mutant embryos (B) present a delay in heart looping at E9.5, in which the ventricle is still at the same dorsoventral position as the atrium. (C, D) At E10.5, the most affected Tbx3 mutant embryos display obvious heart defects, including lack of the constriction in the AVC and absence of looping in a swollen pericardiac cavity (D), compared to wild type (C). (E, F) E11.5 Tbx3 null homozygous embryos present a significant delay in heart looping and pericardiac swelling compared to wild type. (G–O) Whole mount in situ hybridization analysis at E9.5; ventral views (G, H) and lateral views (I–O). (G, H) Upon looping initiation, Bmp10 starts to be expressed in the chamber myocardium. However, Bmp10 was ectopically expressed in the non-chamber myocardium of the heart of Tbx3 mutant embryos (arrow in G, H). (I, J) Bmp2 is expressed in the AVC myocardium at E9.5 and was not altered in Tbx3 mutant embryos. (L, M) TGFβ2 is normally expressed in the non-chamber myocardium of the looping heart. In Tbx3 mutant embryos, expression of TGFβ2 is downregulated in the heart. (N, O) Smad6 is expressed in the endocardium at the level of the OFT and AVC at E9,5. However, in Tbx3 mutant embryos, expression of Smad6 is absent. (P) Consecutive NKE and TBE binding sites are found in the human BMP10 promoter, between 9800 and 10100 bp upstream the ATG codon. a, atrium; lv, left ventricle; o, outflow tract; pc, pericardiac cavity; rv, right ventricle; sa, sinoatrial region.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-3-ponep0000398pg005.jpg"
}
|
001349
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "The pattern of proliferation in the E9.5 heart tube is altered in Tbx3 mutant hearts.Immunohistochemical analysis of cell proliferation in E9.5 wild type (A) and Tbx3 mutant (B) hearts using antibodies against BrdU (A, B) or phospho-Histone 3 (C). (A, B) Representative sections at the level of the AVC of wild type and mutant hearts are shown. The heart is outlined by a discontinuous white line. The density of BrdU positive cells is higher in the AVC of mutant hearts than in the AVC of wild type hearts (red arrowheads). (C) The number of phospho-Histone3-positive cells in the AVC was counted in consecutive heart sections at the level of the AVC of four wild type embryos with 30–31 somites and three mutant embryos with 30–32 somites. The average number of mitotic cells for the AVC is represented in a histogram. The number of mitotic cells in the AVC is significantly increased (P<0.010) in mutant embryos (90.7±12.7), compared to wild type embryos (58.3±14.5). A, atrium; AVC, atrioventricular canal; OFT, outflow tract; V, ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-4-ponep0000398pg006.jpg"
}
|
001350
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Aberrant morphology of the AVC in the absence of zebrafish tbx3b and tbx2a.(A–D) Whole mount RNA in situ hybridization of zebrafish tbx3b and tbx2a expression; ventral views show the heart in its maximum extension from the anterior to the posterior pole. Zebrafish tbx3b is expressed throughout the extent of the heart tube at 31 hpf (A) and becomes restricted to the AVC at 42 hpf (B). Zebrafish tbx2a is expressed al low levels throughout the extent of the linear heart tube at 31 hpf (C). At 42 hpf tbx2a transcripts are present in the AVC at high levels (D). Black arrow points to the AVC. (E–M) Ventral views of the heart of a mlc2a::GFP transgenic line that expresses GFP in the myocardium, at 48 hpf (E–H) and 72 hpf (I–M). (E) In wild type 48 hpf embryos, the atrium has moved upward and is positioned at the same anterior-posterior level as the ventricle; (I) later the atrium becomes localized dorsal to the ventricle at 72 hpf. (F, J) Injection of 2.5 ng of tbx3b morpholino into one-cell stage embryos results in delayed heart looping and abnormal AVC. (G, L) Injection of 10 ng of tbx2a MO results in a similar delay in heart looping and an enlargement of the AVC. (H, M) Injection of both tbx3b and tbx2a MOs, at 1.75 and 5 ng respectively, results in the failure to form the AVC constriction and absence of looping. Red arrow indicates the AVC. a, atrium; h, heart; v, ventricle.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1851989-5-ponep0000398pg001.jpg"
}
|
001351
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Visualization of filament formation of RecA or its variants. Circular ssDNA (10 mM in nucleotides) was pre-incubated with (1 mM) RecA (A) or variants RecA-Htg (B) or RecA-GFP (C) 20 min at 37°C in buffer C containing 20 mM NaCl and 1 mM ATPγS, and incubated for 40 min at 37°C. The specimens were prepared for electron microscopy by negative staining with 1%",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852089-3-1741-7007-5-14-8.jpg"
}
|
001352
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Flagella filaments. Visualization of cell flagella extracted from the leading colony edges grown on swarming plates. (A) Typical swarming cell from the JMG1655 ΔrecA (pRecA-Htg) strain. (B) Typical swarming cells from the JMG1655 ΔrecA (pCA24N) strain. The arrow indicates the position of the flagella in the preparation. Bar 10 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852089-6-1741-7007-5-14-4.jpg"
}
|
001353
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "A) CT scan demonstrating pneumomediastinum. B) CT scan of abdomen demonstrating free intra-peritoneal air.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852095-0-1749-7922-2-8-1.jpg"
}
|
001354
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Patient developed anasarca with rapid progression of Kaposi's Sarcoma after four weeks of taking Imatinib (June 2004).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852096-1-1471-2369-8-6-2.jpg"
}
|
001355
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Gamma plan for the treated brain stem recurrent cystic pilocytic astrocytoma.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852107-0-1477-7819-5-39-5.jpg"
}
|
001356
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Stereotactic MRI brain showed recurrent postoperative brain stem cystic pilocytic astrocytoma.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852107-3-1477-7819-5-39-4.jpg"
}
|
001357
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Gamma Knife treatment planning for the solid parts of the recurrent cystic pilocytic astrocytoma.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852107-4-1477-7819-5-39-1.jpg"
}
|
001358
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "MRI brain, 22 months post gamma knife surgery for brain stem recurrent cystic pilocytic astrocytoma showed marked reduction of the tmuor size.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852107-5-1477-7819-5-39-6.jpg"
}
|
001359
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "General morphology (hematoxolin-eosin; top panels) and labyrinthe endothelial cell staining (isolectin B4; bottom panels) in 12.5 dpc implantation cross sections. ____ = 200 μm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852108-0-1477-7827-5-13-7.jpg"
}
|
001360
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Illustration of fetal development and Ubp43 genotype on day 17.5 of pregnancy (A) and description of how implantation sites were collected (B). A litter from a day 17.5 pregnant mouse shows that all Ubp43 null offspring were dead. Genotype ratio was 2:2:1 (wt:het:null) on day 17.5. On day 12.5, 75% of Ubp43 null mice were dead with a genotype ratio of 2:5:1. There also was a significant loss of +/- fetuses between 12.5 and 17.5 suggesting that Ubp43 gene dosage may also be involved. Panel B shows a cross-sectional representation of a mouse uterus illustrating the various tissues collected for protein and RNA analysis. Anti-mesometrial (AM) represents uterine tissue surrounding the fetal compartment. Fetal chorioallantoic placenta (CP) represents fetal-derived placental tissue. Mesometrial decidua (MM) represents uterine tissue in direct contact with fetal-derived placenta.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852108-2-1477-7827-5-13-1.jpg"
}
|
001361
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Hematoxilin and eosin-stained sections showing solid sheets of squamous cells infiltrating the right lobe of the prostate (a; original magnification 10×) and the left lobe (b, c; original magnification 10× and 20×, respectively). Mytotic activity may also be observed (d; original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852111-0-1748-717X-2-15-1.jpg"
}
|
001362
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Immunohistochemistry showed negativity to cytokeratin 7 (a; original magnification 10×) and cytokeratin 20 (b; original magnification 40×). Squamous carcinoma cells also stained negative for PSA and PAP, while adjacent remaining glands stained positive (c, d; original magnification 40×).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852111-1-1748-717X-2-15-2.jpg"
}
|
001363
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Representative immunohistochemical staining for the selected proteins: E-cadherin, DDR1, DVL1, cytokeratin 5/6, cytokeratin 17 and EMP1. 1.1. E-cadherin is negative in lobular carcinoma; 1.2. E-cadherin is positive in ductal carcinoma; 2.1. DDR1 is negative in lobular carcinoma; 2.2. DDR1 is positive in ductal carcinoma; 3.1. DVL1 is positive in lobular carcinoma; 3.2. DVL1 is negative in ductal carcinoma; 4.1. Cytokeratin 5/6 is negative in lobular carcinoma cells, but its expression is retained in normal ductal epithelial cells; 4.2. Cytokeratin 5/6 is negative in ductal carcinoma cells; 4.3. Duct lobular unit in normal mammary gland tissue is positive for cytokeratin 5/6; 5.1. Cytokeratin 17 is negative in lobular carcinoma cells, but its expression is retained in normal ductal epithelial cells; 5.2. Cytokeratin 17 is negative in ductal carcinoma cells, but its expression is retained in normal ductal epithelial cells; 5.3. Duct lobular unit in normal mammary gland tissue is positive for cytokeratin 17; 6.1. EMP1 is positive in lobular carcinoma; 6.2. EMP1 is negative in ductal carcinoma.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852112-2-1471-2407-7-55-5.jpg"
}
|
001364
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Asporin mRNA detection by chromogenic in situ hybridization. 1–2. Various magnifications (×100, ×400, ×400) of lobular carcinoma; red arrows, lobular tumor cells are positive; blue arrows, stromal cells are negative; 3. Ductal carcinoma is negative (×200).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852112-5-1471-2407-7-55-6.jpg"
}
|
001365
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Colitis development following DSS administration. Representative sections and histological assessment of colonic samples from A) WT and B) VDR KO mice receiving water; C) WT and D) VDR KO mice 5 days after receiving 3.5% DSS; E) WT and F) VDR KO mice 5 days after 2.5% DSS; G) WT and H) VDR KO mice 10 days after 2.5% DSS. Edema (asterisk), cellular inflammation in all layers (arrows).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852118-3-1471-2172-8-5-3.jpg"
}
|
001366
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Immunofluorescence (IF) Analysis of DEK expression. AGN2a (column 2) or AGN2a/DEK (columns 1 and 3) were plated on glass chamber slides, fixed with 4% paraformaldehyde and visualized using a Nuance multispectral imaging system (Nuance 1P46, Cambridge Research and Instrumentation, Inc. Woburn, MA) mounted on a Zeiss Axio Imager Z1 microscope. Image files (200×) were captured and merged using the Axiovision 4.5 package (open program). For all cells, nuclei were stained blue with DAPI. Cells were stained with anti-human DEK antibody (1:100, columns 1 and 2) or similarly diluted isotype control antibody (column 3). Bound antibody was detected with goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 555. Row (A) shows the merged DAPI and Alexa Fluor 555 image, and row (B) shows the staining of the Alexa Fluor 555 image alone. Data is representative of more than three separate experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852119-2-1471-2172-8-4-4.jpg"
}
|
001367
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Electron micrograph showing a number of RNA rotavirus virions (which have a wheel-like appearance) and a number of unknown, 29 nm virion particles",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852121-0-pmedp0040117pg001.jpg"
}
|
001368
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Infectious Rotavirus Is Present in Children with Rotavirus AntigenemiaA total of 11 antigen-positive and nine antigen-negative sera were examined for infectious virus by focus-forming assay after three blind serial passes in HT-29 cells. Infected HT-29 cells were identified using a rabbit antiserum against the ALA strain of rotavirus (A–F) or against NSP4 peptide 114–135 (G, H) followed by a FITC-labeled secondary antibody. Shown are: (A) Rhesus rotavirus (RRV) as a positive control; (B, G) Antigen negative serum; and (C–F, H) antigen positive serum.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852122-2-pmedp0040121pg003.jpg"
}
|
001369
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Cases Where Brain Anomalies Have, or Have Not, Been Linked to Anti-Social Behaviour(A) Brain scan of patient J. S., who exhibited sociopathic behaviour [5]. The image shows a lesion in the orbital frontal cortex.(B) fMRI sagittal slice of the brain of patient J. Z., showing a lesion that was caused by the resection of pituitary tumour [4]. This lesion led to anti-social conduct, which was not exhibited before the surgery.(C) Orbitofrontal damage associated with symptoms of paedophilia and sexual misconduct in the case of a 40-year-old male patient.(D) Photograph of a patient after head injury (right) and fMRI scan 60 years later showing PFC damage (left) [53]. This patient showed personality changes, but no signs of anti-social conduct.(E) Cranial X-ray of a man who attempted suicide with a crossbow. Although the individual exhibited premorbid APD, the PFC damage caused by the crossbow arrow resulted in reversal of anti-social conduct [54].",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852146-0-pbiop0050103pg002.jpg"
}
|
001370
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Regions Associated with Normal and Atypical Social Behaviour(A) Medial and lateral view of the PFC.(B) View of the ventral surface of the PFC and temporal poles.(C) Coronal slice illustrating the amygdalar and insular cortex.See also Table 1.ACC, anterior cingulate cortex; dlPFC, dorsolateral PFC; MFd, medial PFC; oMFC, orbitomedial PFC; TP, temporal pole; vlPFC, ventrolateral PFC; vmPFC, ventromedial PFC.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852146-2-pbiop0050103pg001.jpg"
}
|
001371
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "HepG2 cells were treated with 3.45 μg/ml zerumbone for 24 (B), 48 (C) and 72 (D) hours. DMSO treated HepG2 cells served as negative control (A) and thus gave TUNEL-negative results indicating less apoptotic signal. Arrows indicated cells with fragmented DNA due to apoptosis which occurred actively at the beginning of the treatment and the presence of apoptotic bodies after 72 hours at the end of treatment. Magnification: 1000×.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852295-0-1475-2867-7-4-3.jpg"
}
|
001372
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Immunostaining of Bax protein showed a low level of Bax in the untreated HepG2 cells. However, the immunofluorescence of Bax protein increase and can be seen after 24-h treatment with zerumbone.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852295-6-1475-2867-7-4-7.jpg"
}
|
001373
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "(a) Typical pictures of HCVcc-infected Huh-7 cells observed under a light microscope (×40), showing the absence (on the right) and presence (on the left) of focus-forming units (FFU). (b) A sample-based neutralizing assay, showing the number of FFU in Huh-7 cells following serial dilutions of purified IgG from a HCV-positive serum sample. Neutralizing anti-HCV antibodies titers were expressed as the highest log dilution of IgG producing a 50% reduction in plaque count, as compared with controls in which the dose of virus was known.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852297-2-1743-422X-4-35-1.jpg"
}
|
001374
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Transfection of the hypoblast. A-E: pCAB-Luc monitored by luciferase detection at time points following hypoblast tranfection: 2 hours (A), 5 hours (B), 7 hours (C), 10 hours (D), 14 hours (E). Coloured pixels represent light intensity with blue being least intense and red most intense. F-M: GFP expression in whole embryo- (F,G) and epiblast-transfected (H,I) tissues (fluorescence images, F, H and overlaid with brightfield images, G, I). Arrowheads indicate cells expressing GFP in H. J-M: dsRed and GFP expression in the hypoblast 8 hours (J,K) and 14 hours (L,M) after transfection. An overlay of fluorescence and brightfield images are shown in K and M. N-Q: longer culture periods reveal the transfected hypoblast (shown here with GFP only) in stage HH5 (N), HH7 (O), HH8- (P) and HH9 (Q) embryos. Arrows indicate labelled cells in the yolk sac. R-S: VEcis-Otx2 driving GFP is detected in transfected hypoblasts after 7–8 hours (R) and is seen in the crescent-shaped hypoblast at stage 4+/5 (S). T-U: Co-electroporation of VEcis-Otx2-GFP and CMV-dsRed shows GFP localised in the embryo in the rostral to the hindbrain (T) and also occasionally in the hindbrain (U) (arrows). dsRed is expressed in the entire electroporated region and is seen more caudally in the embryo and in the area opaca (arrowhead).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852305-0-1471-213X-7-25-1.jpg"
}
|
001375
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "In contrast to wild-type ovaries (A, B) homozygous Cx4326/26 knock-in mice reveal only few follicles and no corpora lutea (C). Maturation of follicles appears to be arrested at the early secondary stage (D). The morphology of most follicles was impaired and showed a cyst like structure (D, arrows). Some ovaries consisted of one big cyst (E).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852306-0-1471-213X-7-26-6.jpg"
}
|
001376
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Histological section of the lactating mammary gland of wild-type (A, B) and heterozygous Cx26 knock-in (C, D). The. In contrast to the wild-type mammary glands, heterozygous Cx4343/26 glands show a dramatically reduced branching of the ductuli. As a consequence, less alveoli surrounded by adipocytic tissue were seen (compare A with C). The alveoli were mostly smaller in diameter but did show apocrine secretion and milk droplets in the lumen (arrow) (D).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852306-3-1471-213X-7-26-4.jpg"
}
|
001377
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Tubuli seminiferi (A, B) and ductuli epididymidis (C) of wild-type and Cx4326/26 mice (D-F). Testes of wild-type males revealed densely packed tubuli seminiferi with intact spermatogenensis and ductuli epididymis filled with mature sperms. In Cx4326/26 mice, the seminiferous tubules demonstrated absence of mature sperms but a germinal epithelium. The loss of spermiogenesis was indicated by an empty epididymis with less densely packed tubules. Like in controls (B) immunolabeling of the testosterone receptor revealed the presence of Sertoli (arrows) and Leydig cells in testes of Cx4326/26 mice (E). Primary spermatogonia and spermatocytes type 1 (arrowhead) were present but there was no complete spermatogenesis (E). L, Leydig cells.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852306-6-1471-213X-7-26-5.jpg"
}
|
001378
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "The labeling index is normal at the beginning of cortical neurogenesis, but decreases at late stages in the perlecan-null neocortex. (A-D) Immunofluorescence for BrdU (green) in the cortical primordium after a 30 min (A, B) and a 24 hours (C, D) survival to a BrdU pulse in wild-type (A, C) and perlecan-null (B, D) E16.5 embryos. In both cases, less BrdU+ cells are seen in the perlecan mutants. (E-F) Double immunofluorescence for BrdU (green) and Ki67 (red) in the pallium of E16.5 wild-type and perlecan-null embryos after a 24 h survival to a BrdU pulse at E15.5. Note the panels are high magnification views of the boxed areas in C, D. Observe abundant double-labeled cells (yellow) in the VZ and a thick layer containing BrdU+ cells (and no Ki67+ cells) in the SVZ of wild-type embryos, corresponding to the newly generated neurons (arrow in C and E). Observe the reduced BrdU incorporation in the perlecan-null dorsal cortex, affecting both the VZ and the SVZ. (G) Labeling index (the percentage of BrdU+ cells among Ki67+ progenitors) in cortical sections after 30 min, 4 hours and 24 hours survival to a BrdU pulse at E12.5, E13.5 or E16.5. Means ± SEM values are shown. n = 2 embryos for E12.5; n = 4 for E13.5 BrdU 4 h; n = 5 for E13.5 BrdU 24 h; n = 2 for E16.5 BrdU 30 min; and n = 2 for E16.5 BrdU 24 h. ** p < 0.001. No significant changes of labeling index are evident in the perlecan-deficient embryos at E12.5 and E13.5, but at E16.5 the index is significantly reduced. Scale bars: 100 μm (A-D), 40 μm (E, F).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-0-1471-213X-7-29-3.jpg"
}
|
001379
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Defective neurogenesis in the pallium of perlecan-deficient embryos. (A, B) Double immunofluorescence for Ki67 (red) and BrdU (green) after 24 hours survival to a E12.5 BrdU pulse was used to calculate the fraction of cells exiting the cell cycle (quitting fraction) at E13.5. Cells labeled only with BrdU, a pool no longer dividing, are abundant in the cortical plate in the wild-type embryos (CP in A), but its number in the perlecan-null neocortex decreases considerably (B). (C) Quantification of the fraction of cells leaving the mitotic cycle (quitting fraction: percentage of the number of BrdU+, Ki67- cells among the total of BrdU+ cells) in two E13.5 litters. In the perlecan mutants, the number of cells that leave the cell cycle is reduced to approximately a half of that in wild-type littermates, n = 6 slices.* p < 0.05 and ** p < 0.01. (D, E) Double labeling with phosphorylated histone H3 (red) and BrdU (green) after 2 hours survival to a BrdU pulse, used to compare G2/M phase in wild-type (D) and perlecan-null (E) neocortex at E16.5. (F) Quantification of the percentage of cells in G2/M cell cycle phase (double labeled cells; arrows in D) referred to the total of BrdU labeled cells. n = 12 slices. Scale bar: 40 μm (A, B, D, E).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-1-1471-213X-7-29-4.jpg"
}
|
001380
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Perlecan deficiency increases the cortical progenitor pool. (A-D) Immunostaining for PCNA, a cell cycle marker, in coronal sections of wild-type (A, C) and perlecan-null (B, D) telencephalon at E17.5, Nissl counterstaining. Boxes in (A) and (B) are enlarged in (C) and (D) panels, respectively. (E-H) Nestin expression in coronal sections of wild-type (E, G) and perlecan-null embryos (F, H) at E14.5 (E, F) and E17.5 (G, H). At E14.5, the processes of nestin+ radial glia cells in the marginal zone reach up the pial surface. Note the normal disposition of radial glia endfeet below the basal lamina (BL) in the perlecan-null embryo (F) and in the wild-type (E). Note the marked increase in immunostaining intensity of radial glia in the mutant at both ages. Abbreviations: HIP, hippocampus; nCTX, neocortex; Th, thalamus. Scale bars: 100 μm (A, B), 20 μm (C, D), 30 μm (E, F), 50 μm (G, H).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-2-1471-213X-7-29-5.jpg"
}
|
001381
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Cell proliferation decays in the absence of perlecan. (A, B) Mitotic cells in M phase immunodetected with an anti-phospho-histone H3 antibody in the telencephalic vesicles of E13.5 wild-type (A) and perlecan-null (B) embryos. The neocortex shows two well-differentiated mitotic populations: the ventricular primary population in the VZ, and the basal progenitors in the SVZ that emerges in the middle of the pallium at E13.5. (C) The mitotic index (the percentage of phospho-histone H3-labeled cells among Ki67+ progenitors) shows significant differences in the MGE, but not in the neocortex at E13.5. At E16.5, the mitotic index in the neocortical primordium is reduced to about a 60% of that in wild-type embryos, affecting both the VZ and the SVZ mitotic populations. Values are the mean ± SEM, n = 5 at E13.5; n = 4 at E16.5. * p < 0.05; ** p < 0.001. (D, E) TUNEL staining of E15.5 wild-type (D) and perlecan-null (E) dorsal neocortex. As in the wild-type littermates, very few TUNEL-positive cells (arrows in D and E) were observed in perlecan-null embryos. Scale bars: 150 μm (A, B), 80 μm (D, E).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-3-1471-213X-7-29-2.jpg"
}
|
001382
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Microcephaly in perlecan-null embryos. (A-F) Series of Nissl stained coronal sections of E13.5 forebrain at rostral (A, D), middle (B, E) and caudal (C, F) levels. The brains of a wild-type (A-C) and a perlecan-null (D-F) embryos are shown. Note a reduction in the size of the ventral forebrain in the perlecan-null brains, particularly affecting the medial structures, such as MGE, hippocampus, and rostral part of the thalamus. (G) Quantification of pallial, MGE and LGE cross-sectional area per hemisphere in sections at the middle level. Results reveal that whole brain size is reduced variably in perlecan-null embryos, but that at E13.5 all perlecan-null embryos have severely underdeveloped MGE as compared with their wild-type littermates. Two litters at E12.5 and three litters at E13.5 were used; n = 2 embryos at E12.5 and n = 6 embryos at E13.5. ** p < 0.01. (H, I) Nissl stained coronal sections at E15.5 in wild-type (H) and perlecan-null (I) embryos. Ventral forebrain underdevelopment extends significantly to LGE and paleocortex. Note the reduction of cortical plate thickness in the perlecan-null cortex. (J-M) E17.5 wild-type (J, L) and perlecan-null (K, M) embryos. Panels L and M represent enlargements of the regions boxed in J and K, respectively. The ventral forebrain is reduced in size in the perlecan-null embryo, and the cortical plate is thinner. Additionally, in the perlecan-null telencephalon, the onset of neuronal ectopias (arrows in K) is frequent in the most antero-dorsal region of the cerebral hemispheres. (N-P) Antibodies to laminin highlight the meningeal basal lamina, the meninges and the blood vessels in the wild-type (N) and perlecan-null (O and P) embryos. In the perlecan-null, disruption of the basal lamina is observed in an ectopic area (indicated by arrows in O). However, in the rest of the basal surface of the perlecan-null brain, laminin deposition is continuous (P). Scale bars: 250 μm (A-F, H-I), 500 μm (J, K), 50 μm (L, M), 40 μm (N-P). Abbreviations: CGE, caudal ganglionic eminence; CP, cortical plate; Hp, hippocampus; IZ, intermediate zone LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; P, pallium; Th, thalamus.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-4-1471-213X-7-29-1.jpg"
}
|
001383
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Less neurons and interneurons are present in the cortical primordium of perlecan-null embryos. (A-D) β-tubulin type III immunostaining at E13.5 (A, B) and at E16.5 (C, D). At E13.5, the telencephalic vesicles show immunoreactivity in the mantle of the cortical primordium and of the septal and ganglionic eminences. In the perlecan-null, the extension of β-tubulin immunoreactivity is reduced in the pallium and in the subpallium (arrows in B). At E16.5, neocortical β-tubulin expression is strong in the wild-type subplate (C) but clearly reduced in the perlecan mutants (D). (E, F) At E16.5, the transcription factor Tbr1 labels the nuclei of early-born neurons in MZ, CP and SP. In the perlecan-null embryos the number of Tbr1+ neurons decreases severely and the lower tier of the CP is indistinguishable from the SP (F). Note an ectopia at the right hand side of (F). (G, H) Cortical interneurons detected by calbindin immunohistochemistry at E17.5. Comparable rostro-caudal levels are shown. Note in the perlecan-null brain (H) a notorious descent of the packing density of calbindin-immunoreactive interneurons that have invaded the subplate and cortical plate, as well as the hippocampus. Scale bars: 100 μm (A-D), 40 μm (E, F), 200 μm (G, H). Abbreviations: CP, cortical plate; DTh, dorsal thalamus; Hp, hippocampus; IZ, intermediate zone; POC, primary olfactory cortex anlage; SP, subplate.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-5-1471-213X-7-29-6.jpg"
}
|
001384
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Distribution of Sonic Hedgehog protein in the telencephalon of perlecan-null embryos. (A-H) SHH immunostaining in the forebrain of wild-type (A, E) and perlecan-null (B, F) embryos at E10.5 (A-D) and E12.5 (E-H). C, D, G and H are higher magnifications of the boxed areas in A, B, E and F. In the absence of perlecan, the diffusion of SHH into the brain is still present, but there is a significant decrease in the signal intensity in the ventral telencephalon, especially in the medial ganglionic eminences. Note that the floor plate basal lamina shows a strong SHH immunostaining in the wild-type brain (arrows in C and G) whereas no signal is detectable in the perlecan-null embryos (D and H). (I-K) Laminin immunostaining shows basal lamina continuity in the E12.5 wild-type (I) and perlecan-null (J) floor plate. The deposition of perlecan immunostaining in wild-type embryo coincides with that of laminin (K). The region shown in I-K is the same shown between arrows in (G). (L-O) Immunostaining for Patched 1 (Ptch1), the receptor of SHH, in wild-type (L, N) and perlecan-null (M, O) brains at E12.5. Ptch1 distributes in the mantle of the ganglionic eminences and is absent in the midline, the site of strongest SHH signal. In the mutant there is normal distribution of Ptch1. Scale bars: 100 μm (A, B, E, F, L-O), 50 μm (C, D, G, H), 40 μm (I-K).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852307-6-1471-213X-7-29-7.jpg"
}
|
001385
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Pleomorphic Adenoma of the left parotid gland: MRI axial T1-weighted image, MRI contrast enhanced axial T1-weighted image and MRI axial T2-weighted image",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852309-0-1746-160X-3-19-2.jpg"
}
|
001386
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
|
{
"caption": "Pleomorphic Adenoma of the left parotid gland: Ultrasound image axial and Ultrasound image transversal",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852309-1-1746-160X-3-19-1.jpg"
}
|
001387
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "cye-1 represses the syncytial fate in seam cells.(A and B) Lineages of the T (A) and V6.p (B) cells in wild-type and cye-1(os66) mutants. Sy: syncytial cell. Se: seam cells. Dotted lines indicate the time after hatching the phenotype was scored (8 hrs for T and 20 hrs for V6.p). (C–F) Confocal images of AJM-1::GFP expression. T.ap and V6.pap were outlined by the fluorescence in wild-type animals (C and E) but not in cye-1(os66) mutants (D and F). The lack of AJM-1::GFP signal indicates that these cells fused with the hypodermal syncytium. In cye-1 mutants, daughters of the T.p cell (arrows in D) often did not divide further. Scale bar, 10 µm.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852333-0-ponep0000407pg005.jpg"
}
|
001388
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Transformation of quiescent cells to DTCs after their divisions in cye-1 mutants.(A–K) Anterior is to the left; ventral is to the bottom. Merged GFP and Nomarski images. The gonad is outlined with dotted lines. The original DTC (Z1.aa) and its sister cell (Z1.ap) are marked by an arrowhead and arrow, respectively. The extra lag-2::GFP-positive cells produced from Z1.ap (J) or Z1.p (K) are indicated by asterisks. Scale bar, 10 µm. (A–H) Real-time analyses of lag-2::GFP expression in wild type (A–C), cye-1(os66) mutants (D–F), and hs::cki-1 animals after heat shock (G and H) from the late L1 to early L2 stage. Each vertical set of panels represents the same animal over time. The expression about 3 hours (A and D), 5 hours (B, E and G), and 8 hours (C, F and H) after division of the Z1.a cell is shown. (I–K) lag-2::GFP expression in cdk-2(RNAi) (I) and cki-1(RNAi) animals (J and K).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852333-1-ponep0000407pg002.jpg"
}
|
001389
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Expression of cye-1, cki-1, lit-1 and rnr::GFP in the Z1.a daughters at the late L1 stage.(A–L) Anterior is to the left; ventral is to the bottom. Merged GFP and Nomarski images. The gonad is outlined with dotted lines. Scale bar, 10 µm. The nucleus (A, B, G, H and I–L) or cell membrane (C–F) of Z1.aa (arrowhead) and Z1.ap (arrow) is outlined by white and purple lines, respectively. The expression of CYE-1::GFP in wild type (A) and wrm-1(ne1982) mutants (B). CYE-1::GFP containing the full-length CYE-1 sequence was localized mainly to the nucleus. Expression of cki-1::GFP in wild type (C) and wrm-1(ne1982) mutants (D). cki-1::GFP does not include the cki-1 coding sequence [16] and was expressed in the cytoplasm and nucleus. Expression of cye-1 promoter::GFP (cye-1p::gfp) in wild type (E) and cye-1(os66) mutants (F). Expression of GFP::LIT-1 in wild type (G) and cye-1(os66) mutants (H). Expression of rnr::GFP in wild type (I and K) and cki-1(RNAi) animals (J and L). GFP was detected just after the division of Z1.a (I and J) and disappeared after 2hr in wild type (K), but not in cki-1(RNAi) animals (L).",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852333-2-ponep0000407pg003.jpg"
}
|
001390
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Localisation of D4Z4 and LINE sequences on gorilla (GGO) (A) and chimpanzee (PTR) (B) 4q stretched chromosomes. The reciprocal localisation was derived by dual colour FISH using a gorilla D4Z4 unit (yellow signals) and a LINE probe (red signals). Figures A and B show the hybridisation patterns obtained using a single probe (D4Z4 and LINE inserts), and the merged hybridisation (merged). C) Schematic representation of gorilla (GGO), chimpanzee (PTR) and human (HSA) 4qter genomic organisations. The gorilla and chimpanzee organisations were derived from the molecular and FISH data herein, whereas the human organisation is taken from previous studies (for a review, see van der Maarel et al., 2006). The question mark (?) indicates that the length of the D4Z4 array in chimpanzee was not determined. Double asterisks (**) indicate the overlapping region between the LINE block and subtelomeric block 3 in the human genome [20].",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852401-2-1471-2148-7-39-2.jpg"
}
|
001391
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Maximum intensity z-projections of representative gorilla and chimpanzee fibroblast nuclei after 3D-FISH with BAC 39M12 (green) and a chromosome 4 painting probe (red). The chromosomal counterstain is shown in blue (scale bar = 5 μm). A) and B) gorilla nuclei (A with both alleles located at the nuclear edge, and B with one allele showing a \"track\" of hybridisation signals – arrow); C) chimpanzee nucleus with one 4q35.2 allele abutted against the nuclear envelope.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852401-3-1471-2148-7-39-3.jpg"
}
|
001392
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Brain gene expression levels of Penk (encoding preproenkephalin) and Foxp1 (encoding forkhead box P1). The signal intensities of two genes, Penk and Foxp1, were imported into the NeuroZoom software tool to visualize the three-dimensional gene expression patterns of these genes in the context of brain anatomy. A ratio of the signal intensities of (a) Penk and (b) Foxp1 between 129S6/SvEvTac (129) and A/J (A) strains is shown in hippocampus (Hi), hypothalamus (Hyp), periaqueductal gray (PAG), and bed nucleus of the stria terminalis (BNST). The expression fold change values are shown in the upper right corner of each panel for each brain region separately, together with color coding that matches the color of each brain region in the three-dimensional mouse brain atlas, shown from four different angles. Note that the gene expression level of Penk in Hi and Hyp is higher in the 129 strain than in the A strain, but in Pag and Bnst it is higher in the A strain than in the 129 strain. Similarly, the expression level of Foxp1 in Hi is higher in the 129 strain than in the A strain, whereas in Hyp, Bnst, and Pag the expression level is higher in the A strain than in the 129 strain.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852412-0-gb-2007-8-2-r25-2.jpg"
}
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001393
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Transthoracic echocardiogram on day 5 after admission. Parasternal long-axis view (A) and apical 4-chamber view (B) showing recovery of wall-motion abnormalities with disappearance of apical ballooning.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852545-2-1476-7120-5-18-7.jpg"
}
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001394
|
hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Left ventriculograms. Diastolic (A) and systolic (B) morphology of the left ventricle with the typical appearance of apical ballooning in systole.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852545-4-1476-7120-5-18-4.jpg"
}
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001395
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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|
{
"caption": "Transthoracic echocardiogram on admission. A. Apical 4-chamber view showing apical ballooning of the left ventricle. B. Zoom detail of the same view as in A.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852545-6-1476-7120-5-18-2.jpg"
}
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001396
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Mesodermal differentiation potency of culture-expanded hMSC. Characterization of culture-expanded hMSC upon exposure to specific (A) chondrogenic, (B) osteogenic and (C) adipogenic agents, respectively. Chondrogenic, adipogenic and osteogenic differentiation potential were confirmed by means of (A) immunohistochemical staining of collagen type II fibers, (B) Von Kossa and (C) Oil Red O staining, respectively. 64 × 10 original magnification. Stainings shown are representative for at least 5 separate experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852547-1-1471-213X-7-24-2.jpg"
}
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001397
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Characterization of hepatic differentiation of sequentially TSA- and cocktail TSA-exposed hMSC. Immunofluorescence was performed for HNF3β-cy3, AFP-FITC, ALB-FITC, CK18-FITC, HNF1α-cy3, MRP2-cy3 and C/EBPα-cy3; 32 × 10 original magnification. Stainings shown are representative for at least 4 separate experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852547-2-1471-213X-7-24-5.jpg"
}
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001398
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
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{
"caption": "Differentiation potential of hMSC upon sequential exposure to hepatogenic agents. Upregulated (A) glycogen storage and (B) CK18 expression was shown by means of PAS-staining and immunofluorescence, respectively. A: 10 × 10 original magnification; B: 32 × 10 original magnification. Stainings shown are representative for at least 5 separate experiments.",
"subfigure_path": "/datasets/PMC-15M/filtered_biomedica/filtered_v4/subfigures_final/subfig_0_filelist_commercial_batch_0_111-PMC1852547-4-1471-213X-7-24-3.jpg"
}
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001399
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hf://datasets/vector-institute/open-pmc-18m@b5bf5b815f7ed24176e14a861ca062afe8d8775d/data_00000.tar
|
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