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5,200 |
In Brief
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5,201 |
Preemptive SARS patents
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5,202 |
In the news
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5,203 |
Living and dying with viruses
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5,204 |
Articles selected by Faculty of1000: identifying antigens involved in autoimmune response; statistics in genomics; mouse full-length cDNA encylopedia; screen for Drosophila neural development genes; proteome of SARS-coronavirus
|
A selection of evaluations from Faculty of 1000 covering the identification of antigens involved in an autoimmune reseponse, the difference between false positive and false discovery rate, a mouse full-length cDNA encylopedia, analysis of bristle number in Drosophila mutants to identify neural development genes and an analysis of the proteome of SARS-coronavirus.
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5,205 |
Quick custom flu vaccines
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5,206 |
In the news
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5,207 |
Direct buffer composition of blood pre-process for nucleic acid based diagnostics
|
Recently, a variety of methods, so called “direct buffer”, have been developed to utilize nucleic acid in the blood for the measurement of infectious bacteria and virus without any equipment in the field. In here, we first investigated the individual and combinatory effects of candidate chemicals which might be composed of the direct buffer on the PCR inhibition reduction of main compositions in whole blood. The long and short PEGs, Na(2)SO(4) and GuSCN were selected as representative kosmotropic and chaotropic salts, respectively. MgCl(2) were chosen as divalent cation source and NaOH was used to control blood pH. The effect of common non-ionic biological detergent was tested with Triton X-100 and SDS (Sodium Dodecyl sulfate) was chosen as anionic detergent. These results could provide a foundation for the development of sample preparation solution in nucleic acid based diagnostic field. As a result, the direct buffer developed in this study was able to detect viruses with a concentration of 10(2) pfu/100 μL of whole blood by a very simple method.
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5,208 |
A microbial decade
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In celebration of our tenth anniversary, we revisit selected topics from the first few issues of the journal and assess the progress that has been made over the past decade.
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5,209 |
Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody
|
Severe acute respiratory syndrome (SARS) is caused by the SARS coronavirus (CoV). The spike protein of SARS-CoV consists of S1 and S2 domains, which are responsible for virus binding and fusion, respectively. The receptor-binding domain (RBD) positioned in S1 can specifically bind to angiotensin-converting enzyme 2 (ACE2) on target cells, and ACE2 regulates the balance between vasoconstrictors and vasodilators within the heart and kidneys. Here, a recombinant fusion protein containing 193-amino acid RBD (residues 318–510) and glutathione S-transferase were prepared for binding to target cells. Additionally, monoclonal RBD antibodies were prepared to confirm RBD binding to target cells through ACE2. We first confirmed that ACE2 was expressed in various mouse cells such as heart, lungs, spleen, liver, intestine, and kidneys using a commercial ACE2 polyclonal antibody. We also confirmed that the mouse fibroblast (NIH3T3) and human embryonic kidney cell lines (HEK293) expressed ACE2. We finally demonstrated that recombinant RBD bound to ACE2 on these cells using a cellular enzyme-linked immunosorbent assay and immunoassay. These results can be applied for future research to treat ACE2-related diseases and SARS.
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5,210 |
Death by viroporin
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5,211 |
Structure Watch
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5,212 |
Targeting SARS
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5,213 |
WHO says coronavirus causes SARS
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5,214 |
Conference conundrum
|
Does the popularity of a recent online photonics conference signify a growing appetite for a change in scientific interaction?
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5,215 |
In the News
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5,216 |
Vingt-quatrième réunion du comité local de la Société de pathologie exotique, 18 mars 2014
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5,217 |
In this issue
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5,218 |
A common cure for SARS?
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5,219 |
News review of 2003
|
A month-by-month rundown and follow-up of this year's news highlights in drug discovery
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5,220 |
Reply to “Lack of support for an association between CLEC4M homozygosity and protection against SARS coronavirus infection”
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5,221 |
In Brief
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5,222 |
Unravelling SARS lethality
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5,223 |
In the news
| null |
5,224 |
Some recent patent applications in the area of protease inhibitors
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5,225 |
Awareness and outreach
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5,226 |
SARS resource
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5,227 |
Risikomanagement besonderer Infektionssituationen
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The WHO considers that there is considerable danger of an influenza pandemic. One result of globalisation is that other highly contagious infectious diseases, such as SARS or Ebola, which are potentially endemic, can also be brought into Germany. Bioterorrism must also be considered. As well as all other medical disciplines, hospital surgical departments must be armed against this now. National pandemic planning will be taken as an example to illustrate the planning needed in hospitals to prepare for emergencies arising from infections in such specific situations.
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5,228 |
C-X-C Motif Chemokine 10 Contributes to the Development of Neuropathic Pain by Increasing the Permeability of the Blood–Spinal Cord Barrier
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Neuropathic pain is among the most debilitating forms of chronic pain. Studies have suggested that chronic pain pathogenesis involves neuroimmune interactions and blood–spinal cord barrier (BSCB) disruption. However, the underlying mechanisms are poorly understood. We modeled neuropathic pain in rats by inducing chronic constriction injury (CCI) of the sciatic nerve and analyzed the effects on C-X-C motif chemokine 10 (CXCL10)/CXCR3 activation, BSCB permeability, and immune cell migration from the circulation into the spinal cord. We detected CXCR3 expression in spinal neurons and observed that CCI induced CXCL10/CXCR3 activation, BSCB disruption, and mechanical hyperalgesia. CCI-induced BSCB disruption enabled circulating T cells to migrate into the spinal parenchyma. Intrathecal administration of an anti-CXCL10 antibody not only attenuated CCI-induced hyperalgesia, but also reduced BSCB permeability, suggesting that CXCL10 acts as a key regulator of BSCB integrity. Moreover, T cell migration may play a critical role in the neuroimmune interactions involved in the pathogenesis of CCI-induced neuropathic pain. Our results highlight CXCL10 as a new potential drug target for the treatment of nerve injury–induced neuropathic pain.
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5,229 |
Managing the social amplification of risk: a simulation of interacting actors
|
A central problem in managing risk is dealing with social processes that either exaggerate or understate it. A longstanding approach to understanding such processes has been the social amplification of risk framework. But this implies that some true level of risk becomes distorted in social actors’ perceptions. Many risk events are characterised by such uncertainties, disagreements and changes in scientific knowledge that it becomes unreasonable to speak of a true level of risk. The most we can often say in such cases is that different groups believe each other to be either amplifying or attenuating a risk. This inherent subjectivity raises the question as to whether risk managers can expect any particular kinds of outcome to emerge. This question is the basis for a case study of zoonotic disease outbreaks using systems dynamics as a modelling medium. The model shows that processes suggested in the social amplification of risk framework produce polarised risk responses among different actors, but that the subjectivity magnifies this polarisation considerably. As this subjectivity takes more complex forms it leaves problematic residues at the end of a disease outbreak, such as an indefinite drop in economic activity and an indefinite increase in anxiety.
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5,230 |
Pathology Elsewhere
| null |
5,231 |
Book Review
| null |
5,232 |
The Great Influenza: The Epic Story of the Deadliest Plague in History
| null |
5,233 |
Multisensory processes
| null |
5,234 |
Protein Crystallography in Drug Discovery
| null |
5,235 |
Disinfection, Sterilization, and Control of Hospital Waste
| null |
5,236 |
Liver Disease Associated With Systemic Viral Infection
| null |
5,237 |
Inside Lab Invest
| null |
5,238 |
Pandemic Influenza — Prevention and Treatment: Past, Present and Future
| null |
5,239 |
Book Review
| null |
5,240 |
The social ecology of infectious diseases by Kenneth H. Mayer and H.F. Pizer
| null |
5,241 |
MURINE CYTOMEGALOVIRUS INFECTION OF PHAGOCYTIC LEUKOCYTES
|
Cytomegalovirus (CMV) infections have been associated with altered host defense and increased susceptibility to secondary infection. In previous experiments, we observed impaired migratory and chemotactic activity of neutrophils harvested from mice during sublethal murine CMV (MCMV) infection. To investigate further the effects of CMV infection upon phagocytic leukocytes, we incubated murine leukocyte suspensions (95% neutrophils) in vitro with MCMV derived either from tissue culture passage or salivary gland homogenate. Neutrophils incubated with salivary gland MCMV had depressed chemotactic activity at 2 and 4 h. For example, at 4 h the mean chemotactic index of neutrophils incubated with MCMV was 0.92 vs 2.56 in controls (p<0.01). Phagocytic activity at 4 h was also reduced (mean of 1.53 latex particles/cell in MCMV-incubated vs 2.39 in controls, p<0.01). In contrast, tissue culture-passed MCMV did not alter leukocyte function. Adherence of MCMV to phagocytic leukocytes was comparable to adherence of virus to mouse embryo fibroblast cells (2 to 3 log plaque forming units of MCMV/10(6) cells). Transmission electron microscopy at 4 h demonstrated occasional intracytoplasmic virions. These results indicate that impaired chemotactic and phagocytic activity of phagocytic leukocytes occurs after in vitro incubation with salivary gland-derived MCMV but not with tissue culture-passed virus. These differences may be attributable to altered virulence of MCMV or to the presence of soluble factors in salivary gland MCMV pools
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5,242 |
Management of SARS relies on supportive measures and preventing the spread of infection
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5,243 |
Single Dose Treatments in Tropical Infectious Diarrhoea: The Place of Secnidazole
|
Single dose treatments have the important advantages of simple administration, excellent compliance, short-lived side effects (if any) and short period of drug pressure. However, drugs used for single dose treatments must possess important characteristics, namely, long half-life, effective concentration in tissues over a long period, and low toxicity. In tropical medicine, such drugs are an important advance in the treatment of malaria (mefloquine and halofantrine), schistosomiasis (praziquantel and metrifonate), filariasis (ivermectin), and genitourinary tract and intestinal infections. Intestinal infections are one of the most frequent problems in tropical medicine. While a large number of cases of diarrhoea are of viral (rotavirus) or bacterial (Salmonella, Escherichia coli) origin, necessitating only oral rehydration, some are of parasitological origin (Giardia, Entamoeba histolytica, Strongyloides, Trichuris, Schistosoma) and can be cured by a single dose treatment (tinidazole, secnidazole, ivermectin, albendazole, praziquantel). Secnidazole is the nitroimidazole with the longest half-life and is, therefore, used in single dose treatments for Giardia, acute intestinal amoebiasis and E. histolytica cyst carriers. Single dose treatments of most intestinal parasites will be a major advance in the management of tropical infectious diarrhoea. In the next decade, opportunistic intestinal infections (Cryptosporidium, Isospora, Enterocytozoon) in AIDS patients that are refractory to treatment could be the major problem.
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5,244 |
Extracorporeal membrane oxygenation (ECMO) for pulmonary parenchymal disease in older children
|
Extracorporeal membrane oxygenation (ECMO) for the support of children outside the newborn period who have pulmonary failure is only recently becoming accepted. It is again being applied, after earlier failures, because well-trained teams and improved equipment and techniques are available following the success of neonatal ECMO. In addition, in Europe extracorporeal CO(2) removal (ECCO(2)R) in adults has been more successful. The use of ECMO for pulmonary failure in children does not have fixed indications and has had considerably less success than neonatal ECMO. Patients who require inspired oxygen fractions of over 0.5 and positive end-expiratory pressures of over 6 cm H(2)O for more than 12 h after being treated for more than 48 h should be considered candidates, given the high mortality of children with ARDS (70%). Survival averages 50% to 60%. Circuits and patient management techniques are very similar to those for newborn ECMO, but patients usually require longer times on ECMO. There are many more options for cannulation for both venoarterial and venovenous techniques than in neonatal and cardiac ECMO. The improving results indicate that ECMO will play a part in treating children with pulmonary failure. Further studies will be required to determine which patients can benefit from ECMO as well as the exact application in each case.
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5,245 |
49th Annual Meeting of the Austrian Society of Surgery, Innsbruck, May 21–23, 2008 (Guest Editors: Beate Neuhauser, Dietmar Öfner, Elfriede Ruttmann-Ulmer, and Anton Schwabegger)
| null |
5,246 |
THE PREVALENCE OF CYTOMEGALOVIRUS (CMV) VIRURIA AMONG HOSPITALIZED CHILDREN AND THE RISK OF CMV ACQUISITION BY NURSES
|
In 17 months 2198 children had admission urines cultured for CMV. 4.7% had CMV viruria (105 positive cultures), but significant age differences were observed. In the intensive care nursery the prevalence of CMV viruria (congenital and acquired infections) was 3.3% (18 of 551 newborns with viruria). On the nursing units for children ages 0 to 2 yrs, the prevalence of CMV viruria was 6.8% (32 of 544 positive, P<0.01, Chi square=7.3, when compared to newborns). The prevalence of CMV viruria on a nursing unit for children ages 2 to 5 yrs was 7.2% (27 of 382), similar to that for children 0 to 2 yrs (P>0.5 Chi square=0.004). On a nursing unit for children ages 5 to 12 yrs the prevalence of CMV viruria decreased to 4.2% (20 of 476, P=0.01, Chi square=6.6 when compared to children 2 to 5 yrs). For 12 to 18 year olds the prevalence of CMV viruria was only 1.2% (3 of 245, P<.001, Chi square=9.2, when compared to children 5 to 12 yrs). Among 72 seronegative nurses with daily patient contact, none acquired CMV but only 20 worked with the high prevalence age group (0 to 5 yrs). These results suggest that in spite of constant exposure CMV transmission occurs infrequently.
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5,247 |
39th ESCP European symposium on clinical pharmacy & 13th SFPC congress: clinical pharmacy at the front line of innovations. 21–23 October 2010, Lyon, France
| null |
5,248 |
Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media
|
Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adiposederived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble chondrogenic factors and were effective for directing chondrogenic differentiation of bone marrow stem cells. Nevertheless, it remains unclear whether the conditional media can induce ASCs towards chondrogenic differentiation, especially for three-dimensional (3D) cartilage formation in a preshaped scaffold. In this study, it demonstrated that the conditional media derived from chondrocyte-scaffold constructs could promote ASCs to differentiate into chondrocyte-like cells, with similar expression of type II collagen to those induced by chondrogenic growth factors. Moreover, the expression level of chondrocyte-specific genes, such as SOX9, type II collagen, and COMP, was even higher in conditional medium group (CM) than that in optimized chondrogenic growth factor group (GF), indicating that the conditional media can serve as an effective inducer for chondrogenic differentiation of ASCs. Most importantly, the conditional media could also induce ASC-scaffold constructs to form 3D cartilage-like tissue with typical lacunae structures and positive expression of cartilage specific matrices, even higher contents of GAG and type II collagen were achieved in CM group compared to GF group. The current study establishes a simple, but stable, efficient, and economical method for directing 3D cartilage formation of ASCs, a strategy that may be more closely applicable for repairing cartilage defects.
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5,249 |
Autopsy
| null |
5,250 |
Techniques
| null |
5,251 |
Special Issue: Abstracts of the 9(th) International Conference on Environmental Health Science, 2-3 November 2016, Incheon, Republic of Korea: Global Issues in Marine Environmental Conservation and Health Diagnosis November 2 (Wed) – 3 (Thu), 2016 Ghent University Songdo Global Campus Incheon, Republic of Korea
| null |
5,252 |
Pflegepolitik in 5 Minuten
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5,253 |
Es gibt auch noch etwas Anderes
| null |
5,254 |
Mitteilungen der DGIM
| null |
5,255 |
Role of Viral Infection in the Aetiology of Multiple Sclerosis: Status of Current Knowledge and Therapeutic Implications
|
Multiple sclerosis (MS) is a chronic disease of the CNS that typically begins in late adolescence or early adulthood. It is highly variable in its expression and severity. The cause of MS is unknown, but both genetic and environmental factors have been implicated in its pathogenesis. It is known that viruses can induce chronic neurological disease, but the pathogenetic process in unclear. A viral cause for MS has been postulated, but to date no single virus has been confirmed to be associated with the disease. Although most viral candidates are no longer considered as possible aetiological agents in MS, a few are still being investigated.
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5,256 |
Sustaining Hospital Readiness for Ebola
| null |
5,257 |
Literature in pediatric radiology
| null |
5,258 |
European Society of Pediatric Radiology 32nd Congress Utrecht, The Netherlands, May 18–20,1995 Abstracts of Oral Presentations and Poster Presentations
| null |
5,259 |
Posters
| null |
5,260 |
Book review
| null |
5,261 |
Differentially expressed genes in human gingival fibroblasts cultured on microgrooved titanium substrata: A pilot study
|
The purpose of this study was to determine the differentially expressed genes in human gingival fibroblasts (HGFs) cultured on titanium (Ti) substrata with topographies presenting microgrooves and acid-etched roughness. Microgrooves were fabricated with a truncated V-shape in cross-section at 15/3.5, 30/10, and 60/10 μm (width/depth) by photolithography. Subsequent acid etching was applied to the entire surface of the fabricated Ti substratum to generate etched microgrooves and ridges (designated as E15/3.5, E30/10, and E60/10). Both smooth and acidetched-only Ti were used as controls (designated as NE0 and E0). Large-scale gene expression analyses were performed using differential display PCR, and the results were confirmed using RT-PCR and quantitative real-time PCR. Of the 21 genes with altered expression determined by differential display PCR and sequencing, we verified through RT-PCR that MTDH and TIMP1 were up-regulated and TGF-β1, TPM1, and VIM were down-regulated in the HGFs cultured on E60/10 versus NE0. We also confirmed, by quantitative real-time PCR, that MTDH and TIMP1 expression in HGFs on E60/10 was significantly up-regulated compared to HGFs on the other Ti substrata. This study indicates that acid-etched ridges and microgrooves on Ti with a width and depth of 60 and 10 μm (E60/10) induce alterations in the expression of genes involved in cell adhesion, proliferation, and regulation of the cytoskeleton in HGFs.
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5,262 |
Canicules, La Santé Publique en Question
| null |
5,263 |
Book review
| null |
5,264 |
Book review
| null |
5,265 |
Mixed and blended emotional reactions to 2014 Ebola outbreak
| null |
5,266 |
CMV reactivation caused by methylprednisolone therapy for ARDS after esophagectomy
|
BACKGROUND: Cytomegalovirus (CMV) infection is endemic worldwide. Although CMV reactivation often becomes a serious problem in immunocompromised patients, the prevalence of CMV reactivation caused by methylprednisolone therapy for ARDS after esophagectomy has yet to be determined. METHOD: Among 175 consecutive patients with thoracic squamous cell esophageal cancer who underwent esophagectomy with extensive lymph node dissection at Akita University Hospital between 2007 and 2010, 11 patients (6.3 %) diagnosed with ARDS during the acute phase of esophagectomy were enrolled and treated with steroid pulse therapy, high-dose (15–20 mg/kg/day) administration and tapering in this retrospective study. RESULTS: Seven of the 11 patients (63.6 %) were diagnosed with CMV reactivation based on CMV antigenemia assayed 19.1 days after the start of methylprednisolone administration and were treated with ganciclovir for 39.6 days. Six of the 7 patients (85.7 %) diagnosed with CMV reactivation were administered a total methylprednisolone dose of more than 4,000 mg. Though there was no significant difference between patients with and without CMV reactivation, there was a tendency that patients with CMV reactivation showed a lower minimum number of lymphocytes during the acute phase of esophagectomy (p = 0.051, Student’s t test, average 223.3 and 298.0/μl, respectively). CONCLUSION: Though the number of study patients is small, the prevalence of CMV reactivation caused by high-dose methylprednisolone therapy for ARDS after esophagectomy is remarkably high. This result strikes a note of warning concerning the management of these patients and suggests the importance of screenings for CMV reactivation so as to make an accurate diagnosis and initiate treatment in a timely manner.
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5,267 |
Abstracts of the 35th annual meeting of the GTH
| null |
5,268 |
43. Jahrestagung der Österreichischen Gesellschaft für Pneumologie. 3. Jahrestagung der Österreichischen Gesellschaft für Thoraxchirurgie
| null |
5,269 |
Inside the USCAP Journals
| null |
5,270 |
25. Jahrestagung der Deutschen Gesellschaft für Hämatologie und Onkologie gemeinsam mit der Österreichischen Gesellschaft für Hämatologie und Onkologie: Kongreß 1980, Homburg/Saar, 5. bis 8. Oktober 1980 Referate 1–32, Freie Vorträge 33–140
| null |
5,271 |
XVI meeting of the European Association for Haematopathology 2012 Abstracts
| null |
5,272 |
Selected Abstracts from the National Congress of the Italian Society of Hypertension (SIIA 2009): 2009 October 1–4, Rome, Italy
| null |
5,273 |
Abstracts presented at the 13th International Congress on Amino acids, peptides and proteins
| null |
5,274 |
Stomata and plasmodesmata
|
In developing epidermal tissue ofPhaseolus vulgare L. complete plasmodesmatal connections occurred between guard cells and epidermal cells and between sister guard cells of a stoma but they were not seen in fully differentiated tissue. However, incomplete, aborted plasmodesmata were occasionally seen in the common guard/epidermal cell wall, usually connected to the epidermal cell protoplast, in mature tissue. Plasmodesmatal connections between neighbouring epidermal cells were commonly observed in tissue at all stages of development. In all locations, the plasmodesmata were usually unbranched occurring singly or in small pit fields; very rarely branched, incomplete plasmodesmata were also seen in the wall between mature guard and epidermal cells. The significance of these findings were related to stomatal functioning and to the development of plasmodesmata in general.
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5,275 |
PP4 - Displayed on Wednesday, September 12
| null |
5,276 |
Abstracts of papers and posters advanced activities in pharmaceutical care 24th European Symposium on Clinical Pharmacy
| null |
5,277 |
Hinweise auf Artikel in dieser Ausgabe
| null |
5,278 |
Abstract of the 68th Meeting (Spring Meeting) 6–9 March 1990, Heidelberg
| null |
5,279 |
BHS Abstracts
| null |
5,280 |
Abstracts for the IPNA Congress, 30 August - 3 September 2013, Shanghai, China
| null |
5,281 |
The pathogenesis of inflammatory disease: Surgical shock and multiple system organ failure
|
Chronic inflammatory disease, embracing rheumatoid arthritis (RA), inflammatory bowel disease (IBD), hepatitis, asthma, atherosclerosis, multiple system organ failure (MSOF), etc., is mediated by reactive oxygen species (ROS). These ROS originate from activated neutrophils in infections and in immune and autoimmune reactions, from tissue deposits of ferritin, and from futile cycling of cytochrome P450 (CYP) following exposure to persistent chemicals, and may be perpetuated by the actions of complement, cytokines and eicosanoids. Acute inflammation is normally arrested by removal of ROS by tissue glutathione (GSH) and the antioxidant vitamins, A, C and E, all of which are regenerated by NADH and NADPH. Failure of this antioxidant defence system can lead to oxidative stress and to chronic inflammatory disease, including surgical shock and MSOF. The roles of oxidative stress and microcirculatory arrest in promoting MSOF, and of GSH, the antioxidant defence system, and fibronectin in preventing this, are reviewed in the light of recent experimental studies of surgical shock, including fasting, anaesthesia, hepatic ischaemia and reperfusion.
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5,282 |
43. Jahrestagung der Österreichischen Gesellschaft für Innere Medizin
| null |
5,283 |
Kardiopulmonale Reanimation „oben ohne“. Mode oder Wissenschaft
| null |
5,284 |
AUTHOR INDEX
| null |
5,285 |
Abstracts of the 37th annual meeting of the GTH (Gesellschaft für Thrombose- und Hämostase-forschung)
| null |
5,286 |
Transgenesis and genome analysis, Nantes, France, June 6th 2011
| null |
5,287 |
Subject Index to Volume 35
| null |
5,288 |
Traumatologie II
| null |
5,289 |
Viruseinschlüsse in der Zellwand und in Protoplasten vonin vitro kultiviertenArmoracia-Geweben
|
Root explants ofArmoracia rusticana were cultivatedin vitro on theMurashige, andSkoog 1962 nutrient medium with addition of 2,4 D (2,4-Dichlorophenoxyacetic acid). Under such conditions callus tissue containing several apical meristems developed. Without 2,4 D the explants were growing to aseptic small plants; when further cultivated in soil they developed into big plants showing leaves with different deformations. These leaves, aseptically cultivated plants and callus tissue were examined by light and electron microscopy. There occured two types of inclusions consisting of isometric and elongated particles, respectively. Those of the isometric type were observed in the cytoplasm of meristematic cells of the callus; the isometric particles had a diameter of about 20–22 nm and were arranged hexagonally or in curvilinear arrays. In all other tissues that were examined such aggregates were to be seen only in vacuoles. Isometric particles were also found in plasmodesmata of all tissues examined. They lay in a tubulus which protrudes into the cytoplasm on one side of the cell wall. Desmotubuli appeared only in particle-free plasmodesmata. Protrusions of the cell wall into the cytoplasm containing tubuli and isometric particles within were observed only in callus tissue and in epidermic cells of leaves. In the cell wall of callus cells, conspicuous cavities connected with the cytoplasm by plasmodesmata contained many particles which were arranged in hexagonal structures. It seemed as if they had been put away into the cell walls or into the vacuoles. The occurence of fibrillar inclusions together with those consisting of isometric particles in the same cell was extremely rare. The fibrillar inclusions were observed in the cytoplasm and in the nucleus. The diameter of one fibril is about 12 nm. The thylacoids in well differentiated chloroplasts in callus and in cells of leaves are curved; single swelled thylacoids lie as vesicles in peripheral stroma. As tissues ofArmoracia rusticana plants from different localities do not contain such inclusions we assume that the root segment examined was infected with two different viruses. The isolation of the viruses has not yet been possible.
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5,290 |
Irish thoracic society: Proceedings of Meeting held November, 1691 in Belfast
| null |
5,291 |
Author and Subject Indexes—Volume 27, 2003
| null |
5,292 |
Abstracts, IAP'98 8th Meeting of the International Association of Pancreatology July 13–15, 1988 Tokyo, Japan
| null |
5,293 |
Inside the USCAP Journals
| null |
5,294 |
Jahrestagung 2011 der Österreichischen Gesellschaft für Pneumologie
| null |
5,295 |
Book review
| null |
5,296 |
35th Annual Meeting of the Arbeitsgemeinschaft für Pädiatrische Nephrologie, 18 - 20 March 2004, Mainz, Germany
| null |
5,297 |
Inaugural national scientific medical meeting
| null |
5,298 |
Scientific Programme
| null |
5,299 |
Abstracts of the contributed papers
| null |
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