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3500ea95-abeb-4a62-8936-5fe94c01ea13
| 3 |
%.
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5960bf24-21c1-4fee-a695-e2298988075a
| 0 |
[The metabolism of delta4-3-oxosteroids in rat liver]. The enzymatic reactions are described by which delta4-3-oxosteroids, specially testosterone, are inactivated in rat liver. The delta4-3-oxosteroid-5 alpha-reductase in liver microsomes was studied intensively and it was found that it is an enzyme system. The 5 alpha-reduction of testosterone with NADPH or with NADH depends upon different enzymes or enzyme systems.
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e9076ba6-4dab-4000-af07-dc910fc2ef49
| 0 |
[An improved trihydroxyindole method for the determination of urinary catecholamines]. Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescence of adrenaline or noradrenaline was enhanced, in comparison with other methods, by the combined application of boric acid, copper-ions, mercaptoethanol and final reacidification.
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e9076ba6-4dab-4000-af07-dc910fc2ef49
| 1 |
The fluorophores are stable: The loss of fluorescence of adrenolutin amounted to 22% during 180 minutes, and there was no loss of noradrenolutin. For the differentiation of amines, adrenaline was oxidized at pH 2.85 and noradrenaline at pH 7. Precision, accuracy, sensitivity and specifity fulfilled the criteria of analysis.
|
e9076ba6-4dab-4000-af07-dc910fc2ef49
| 2 |
The normal values, determined in a collective of 17 healthy persons, were adrenaline (x +/- s) 44.8 +/- 16.9 nmol/24 h and noradrenaline (x +/- s) 224.0 +/- 68.0 nmol/24h. The simultaneous application of alpha-methyldopa (2 g/day) in 13 patients with primary hypertension did not disturb the fluorimetric estimation of catecholamines.
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467cf9de-71e7-42d1-bc03-659ac100223b
| 0 |
[Biochemical investigations of cancer cachexia. II. Depletion of glycogenolysis and stimulation of gluconeogenesis in Walker carcinoma 256 bearing rats (author's transl)]. 150-200 g heavy, Walker-carcinoma bearing, male Sprague-Dawley-rats showed rapid, tumour weight dependent, loss of liver glycogen until complete depletion in tumour groups heavier than 40 g/animal.
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467cf9de-71e7-42d1-bc03-659ac100223b
| 1 |
Simultaneously the glycogen mobilization after massive glucagon stimulation, was successivly diminished and finally abolished in different groups with increasing tumor weight. Concomitantly the spontaneous and stimulated activity of liver phosphorylase a was found markedly reduced in advanced tumour cachexia, the extent of stimulation of liver phosphorylase a activity by intracardial injections of epinephrine not being altered.
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467cf9de-71e7-42d1-bc03-659ac100223b
| 2 |
Tumour induced inhibition of glycogen mobilization thus appears to have been excluded. To account for the relative late pronounced hypoglycemia in peripherial rat blood in face of the early loss of liver glycogen, accelerated gluconeogenesis has been postulated. In accord with this spontaneous rise in liver tyrosine amino transferase was found in tumour bearing rats along with a doubled maximal stimulation value after medrol injection as compared to control groups.
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467cf9de-71e7-42d1-bc03-659ac100223b
| 3 |
This behavior could not be shown for liver alanine aminotransferase and liver fructose 1,6-di-phosphatase. The former showed no differences between control and tumour groups neither of spontaneous nor of stimulated activity. The latter showed only a very reluctant rise after massive stimulation by triamcinolone for 3 days in the control groups, the tumour bearing groups showing no deviation from spontaneous control values.
|
3144cd71-2bfc-4d19-9742-aadcf220ff86
| 0 |
Oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases under in vivo and in vitro conditions. Oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. All peroxidases obtained from soybean (Glycine max) cell suspension cultures by gel electrophoresis are equally capable to decarboxylate p-hydroxygenzoic acids as indicated by their rather low differences in specific activity for various benzoic acids.
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faba7872-89c0-4cba-8e4a-f16fe221665f
| 0 |
The regulation of glucose-6-phosphate dehydrogenase in chloroplasts. Glucose-6-phosphate dehydrogenase from intact pea chloroplasts is partially membrane bound and inactivated upon illumination. The inhibitory effect of light can be abolished by addition of methylviologen. Kinetic experiments with glucose-6-phosphate dehydrogenase reveal that, in the dark, the enzyme activity is strongly inhibited by the accumulation of NADPH.
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faba7872-89c0-4cba-8e4a-f16fe221665f
| 1 |
The inhibition of NADPH can be reversed by the addition of excess NADP+. The non-Michaelis-Menten-type kinetics suggest that the enzyme is stringently regulated by the ratio of NADPH to NADP+ plus NADPH, i.e., the "reduction charge". These observations seem to indicate that in the light the inhibition of glucose-6-phosphate dehydrogenase is due to a high reduction charge, whereas in the dark the enzyme is controlled by the metabolic demand for reducing equivalents.
|
f1eb5d47-d858-438c-9023-e0bd766f041f
| 0 |
Characterization of different deoxyribonucleases in human lymphocytes. Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribonuclease found in the cytoplasmic fraction prefers native or UV-irradiated DNA over denatured DNA as substrate and is a 5'-monoester former.
|
f1eb5d47-d858-438c-9023-e0bd766f041f
| 1 |
Two groups of acid deoxyribonuclease activities are detectable in the nuclear fraction. Both are 3'-monoester formers. One is as well active with denatured DNA as with native DNA, the other one shows the same activity with native and UV-irradiated DNA but lower activity with denatured DNA.
|
f1eb5d47-d858-438c-9023-e0bd766f041f
| 2 |
An alkaline deoxyribonuclease activity, also localized in the nucleus, is a 5'-monoester former, and prefers denatured or UV-irradiated DNA as substrate.
|
1c92cbc7-ad79-43f2-94b1-56e0f91d8dc2
| 0 |
Studies on fragments of rod outer segments from bovine retinas. Monolayers of fragments of our rod segments were formed at an air-water interface. The area of these particles was measured as a function of the pH of the aqueous phase. A maximum area was measured at pH 6.5
|
1c92cbc7-ad79-43f2-94b1-56e0f91d8dc2
| 1 |
. The film was characterised by measuring the decrease in surface pressure, after compression, and the half time required to reach constant pressure. At a pH between 6 and 7 the decrease in surface pressure was a minimum. Irradiation of films of fragments resulted in a decrease in surface potential of between 30 and 40 mV.
|
1c92cbc7-ad79-43f2-94b1-56e0f91d8dc2
| 2 |
The largest decrease occured at a pH between 6 and 6.5. A small decrease in surface pressure was also observed upon irradiation. Mixed films of fragments and phosphatidyl ethanolamine were examined. Irradiation of the mixed film resulted in a 70 mV decrease in surface potential.
|
01ffd781-604f-468a-8744-c4478e659bc0
| 0 |
The ionization and optical properties of oxoformycin. Details are given of the ultraviolet, optical rotatory dispersion and fluorescence spectra of oxoformycin. The pK of formation of the monoanion is 8.6, at 25 degrees C. As formycin B has the same pK value, the ionization of both compounds is ascribed to dissociation of a proton from N-1. Both the neutral and monoanionic forms of oxoformycin are strongly fluorescent.
|
eba906b4-a652-470a-b299-6583ba6c8e26
| 0 |
Antagonistic relationships between electron transport and P700 in chloroplasts and intact algae. The effects of divalent salts and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) upon nicotinamide adenine dinucleotide phosphate (NADP) reduction and P700 in isolated chloroplasts are described and compared with the influence of DCMU on oxygen evolution and P700 in intact cells.
|
eba906b4-a652-470a-b299-6583ba6c8e26
| 1 |
Most experiments were carried out with a steady-state relaxation spectrometer. A kinetic mechanism for the estimation of P700 fluxes is proposed and experimentally tested. Good agreement between theory and experiment was found. Concurrent measurements of P700 and NADP reduction revealed two antagonisms: addition of divalent cations caused an increase in the yield of NADP reduction and a decrease in the yield of P700.
|
eba906b4-a652-470a-b299-6583ba6c8e26
| 2 |
Conversely, in the presence of Mg2+ low concentrations of DCMU decreased the yield of NADP reduction and increased the yield of P700. Aging of the chloroplasts at 30 degrees C exerted a similar effect. With far-red actinic light, Mg2+ stimulated the yield of NADP reduction without affecting the flux.
|
eba906b4-a652-470a-b299-6583ba6c8e26
| 3 |
Also, in the absence of Mg2+, DCMU inhibited both reactions although P700 required a higher herbicide concentration for fractional inhibition than NADP reduction. In the presence of Mg2+, chloroplasts resembled intact algae in which a high rate of oxygen evolution was accompanied by little P700 turn-over.
|
eba906b4-a652-470a-b299-6583ba6c8e26
| 4 |
Titration with DCMU decreased the rate of photosynthesis and increased P700 flux. On the whole, the data suggest that P700 relaxing in 20 msec is not directly involved in linear electron transport.
|
4dbdaae0-c493-4cf9-9978-c36076edcef3
| 0 |
Energy-linked pyridine nucleotide transhydrogenase activity in photosynthetically grown Rhodopseudomonas palustris. Rhodopseudomonas palustris (ATCC 17001) develops energy-dependent NADP+ transhydrogenase activity while growing photosynthetically on thiosulfate, formate, or acetate as the electron donors. The enzymatic activity is present in the supernatant fraction S-144 000. -- As reported, this fraction contains small membrane fragments but no closed vesicles and was shown to drive energy-dependent reversed electron flow as well as an aerobic respiratory electron transport.
|
4dbdaae0-c493-4cf9-9978-c36076edcef3
| 1 |
The energy-dependent transhydrogenase reaction in this fraction can be driven either by ATP, ADP, or inorganic pyrophosphate, but also by acetyl phosphate or acetyl-coenzyme A in the presence of orthophosphate. -- Arsenate acts as an inhibitor and decreases preferentially the acetyl-coenzyme A-dependent and the acetyl phosphate-driven reaction;
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4dbdaae0-c493-4cf9-9978-c36076edcef3
| 2 |
whereas, oligomycin inhibits preferentially the ATP- and the acetyl phosphate-dependent reactions. -- Acetate kinase and a phosphotransacetylase are operative in S-144 000.
|
b8e57558-c7a6-4cbc-af20-3faf81679593
| 0 |
[Findings of subpartal monitoring, status of the newborn infant and evaluation of cardiotocographic findings]. The parameters of the intrapartal fetal CTG and the actual pH are related to each other and to the Apgar score of the newborn. The interpretation was carried out by means of groups of 300 to 600 cases.
|
b8e57558-c7a6-4cbc-af20-3faf81679593
| 1 |
The actual fetal pH has the most compact relation to the Apgar score of the newborn (C equals 0,31). But also this contingence is unsatisfactory. At fetal acidosis most of the Apgar scores are pathological, in that manner the pH is the most important test for indication of operative delivery.
|
b8e57558-c7a6-4cbc-af20-3faf81679593
| 2 |
The rare CTG pattern as dip II and variable decelerations combined with tachycardia of bradycardia, the bradycardia with 80 bpm or less, are likewise indications of urgent delivery.
|
c154a922-f447-4882-b99d-99bcbcf4c179
| 0 |
[Influence of low molecular dextran on the acid-base balance in mother and fetus]. 9 normal pregnant women in the active phase of labor were treated with 500 ml low molecular dextrane with a pH of 4,5 under standardised external conditions. Before and after this infusion simultaneous estimations of the maternal and fetal acid-base status were made. There was neither a maternal nor a fetal acidosis.
|
81c704c3-6a45-41a0-930c-ca030fae5918
| 0 |
[Acetyl-CoA-synthetase activity of pigmented staphylococci]. In 19 strains of staphylococci a study was made of the activity of acetyl-CoA-synthetase reaction. All the strains possessed an active enzymatic system transforming the acetate into an active form. The activity of acetyl-CoA-synthetase proved to be much greater in the pigmented staphyloccus strains than in the nonpigmented ones. It is supposed that there existed an association between the acetyl-CoA-synthetase and the biogenesis of carotinoid pigments in Staph. aureus.
|
8d60bca3-ae22-4e68-a743-b5b194f3eb75
| 0 |
[Acetylcholinesterase from the electric organ of the ray Torpedo marmorata]. Studies have been made on substrate specificity of acetylcholinesterase (AChE;EC 3-1-1-7) from the electric organ of the ray T. marmorata with respect of choline and thiocholine esters, as well as on the effect of pH, salts and organophosphorus inhibitors (OPI) on the activity of the enzyme.
|
8d60bca3-ae22-4e68-a743-b5b194f3eb75
| 1 |
Acetylcholine (ACh), propionycholine (PrCh) acetyl-beta-methylcholine (MeCh), acetylthiocholine ((ATCh) and propionylthiocholine (PrTCh) were hydrolyzed by the enzyme studied at the following relative rates-100: 28.8: 18.3: 87.2: 18.9 correspondingly. In all the cases, inhibition of the enzyme by high concentrations of the substrate was observed.
|
8d60bca3-ae22-4e68-a743-b5b194f3eb75
| 2 |
As compared to other AChE, the enzyme from T. marmorata exhibits the highest affinity to ACh. For all the substrates studied, pH dependence of AChE activity followed the curve with maximum 7.5 for ACh and PrCh, 8.0-8.5 for ATCh and MeCh and 7.5-8.5 for PrTCh.
|
8d60bca3-ae22-4e68-a743-b5b194f3eb75
| 3 |
Various salts (MgCl2), KCl, NaCl, NaBr, KI) increased AChE activity, the increase being the highest with MgCl2 (3.3 times) and NaCl (2.5X). Biomolecular rate constants ((k) II) for the interaction of AChE investigated with OPI containing cationic group-methylsulfomethylates, O-ethyl-S-(beta-ethylmercapto) ethylmethylthiophosphonate and O,O-diethyl-S-(beta-ethylmercapto) ethylthiophosphate, as well as methyl iodide O,O-disopropyl-S-(beta-phenylmethylamino) ethylphosphate-were significantly higher as compared with k(II) values for corresponding compounds without the cation.
|
8d60bca3-ae22-4e68-a743-b5b194f3eb75
| 4 |
The value of k(II) sharply decreased with the increase in the size of the acyl radicals at phosphorus atom in the molecule of OPI.
|
0b6b0d2e-fb92-46c4-9b08-402aa01e1a74
| 0 |
Isolation and properties of tRNA nucleotidyltransferase from wheat embryos. From wheat embryos, tRNA nucleotidyltransferase (EC 2.7.7.25) was isolated. By chromatography on Sepharose 6B, DEAE-cellulose and affinity chromatography on tRNA-hydrazyl-Sepharose 4B, 7000-fold purification of the enzyme was achieved. The enzyme required for its activity Mg2+ or Mn2+ ion.
|
0b6b0d2e-fb92-46c4-9b08-402aa01e1a74
| 1 |
ATP inhibited incorporation of CMP from CTP into lupin tRNA, and CTP acted as a competitive inhibitor of AMP incorporation from ATP. The regulatory role of ATP in incorporation of terminal CMP into tRNA is discussed. The incorporation of terminal CMP into tRNA deprived of terminal CCA or CA, was also studied.
|
6e75fe72-dd90-41e2-bfea-54e7b32615f6
| 0 |
[Electronmicroscopic study of the gravity receptors in ctenophores]. Secondarily sensitive receptor balancer cells comprise the gravity receptor in the aboral organ-polivalent organ of Ctenophora. Ctenophora are phylogenetically the first animals which created receptor cell supplied with a single flagellar kinocilium connected with the otolithic apparatus. The receptor balancer cells are innervated by the nerve fiber branched off the nerve cells of the aboral ganglion.
|
6e75fe72-dd90-41e2-bfea-54e7b32615f6
| 1 |
The localization of AChE, BuChE products of cytochemical reaction on sodium suggests that the cholinergic mechanism may take part in the process of synaptic transmission. Our morphological data cannot exclude an adrenergic mechanizm also.
|
2531c8d1-8699-4196-a2a7-4bd14556daf2
| 0 |
Non-specific acetyl-CoA carboxylase and methylmalonyl-CoA carboxyltransferase in Streptomyces noursei var. polifungini. 1. Acetyl-CoA carboxylase (EC 6.4.1.2) and methylmalonyl-CoA carboxyltransferase (EC 2.1.3.1) have been isolated from mycelia of Streptomyces noursei var. polifungini, and purified about 50-fold. 2. Both enzymes carboxylate acetyl-CoA and propionyl-CoA;
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2531c8d1-8699-4196-a2a7-4bd14556daf2
| 1 |
the respective Km values are 1.1 and 1.6 mM with acetyl-CoA carboxylase and 2.5 and 1.25 mM with carboxyltransferase. 3. The activities of both enzymes are inhibited by free fatty acids. Almost total inhibition of methylmalonyl-CoA carboxyltransferase was observed by 0.1 mM-butyrate or 0.1 mM-C14-C18 acids.
|
2531c8d1-8699-4196-a2a7-4bd14556daf2
| 2 |
Acetyl-CoA carobxylase was affected to the same extent by these compounds at concentration of about 1 mM. 4. The role of both carboxylating enzymes is biosynthesis of the antibiotic is discussed.
|
71aa5473-3e32-4341-bcf6-aac43c27c39d
| 0 |
Preoperative treatment of thyrotoxicosis with a beta-adrenergic blocking agent. The clinical usefulness of propranolol as the sole drug in the preoperative treatment of thyrotoxicosis has been investigated. The effect of the drug on the serum levels of thyroxine and triiodothyronine has also been studied. Eight consecutive patients were treated.
|
71aa5473-3e32-4341-bcf6-aac43c27c39d
| 1 |
Four of these showed good clinical response to propranolol and both pre- and postoperative periods were uneventful. Two patients also showed a good clinical response but developed accentuated thyrotoxic symptoms in the immediate postoperative period. One patient showed no clinical response to propranolol therapy and in one further patient the treatment was discontinued because of discomfort.
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71aa5473-3e32-4341-bcf6-aac43c27c39d
| 2 |
All glands were resected without technical difficulty. They were more vascular and friable after this therapy than after pretreatment with antithyroid drugs and thyroxine. The serum levels of thyroxine and triiodothyronine decreased during propranolol treatment. It is concluded that treatment with propranolol as a sole preoperative drug might be used as an alternative only in cases where conventional therapy is not suitable.
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b70c597d-d500-4607-bef6-d0967eed03d4
| 0 |
Reduction of 3-oxosteroids in human liver microsomes. The 3alpha- and 3beta-reduction of the following steroids was studied in human liver microsomes: 5alpha-androstane-3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5alpha-pregnane-3,20-dione, 5beta-pregnane-3,20-dione, 3-oxo-5beta-cholanoic acid and 7alpha-hydroxy-5alpha-cholestan-3-one.
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b70c597d-d500-4607-bef6-d0967eed03d4
| 1 |
With NADH as cofactor there was a preferential 3alpha-reduction of the C19- and C21-3-oxo-steroids and a preferential 3beta-reduction of the C24- and C27-3-oxo-steroids. Substitution of NADH with NADPH influenced reduction of the substrates in different ways, indicating the presence of several 3alpha- and 3beta-hydroxysteroid dehydrogenases with different substrate specificity and specificity towards NADH and NADPH.
|
b70c597d-d500-4607-bef6-d0967eed03d4
| 2 |
Only small or insignificant sex differences could be observed in the reductions studied.
|
1482c2a7-8336-4d2b-a63f-74d78ccb7376
| 0 |
The fractional post-coital test performed in a square capillary tube. A new method of examination of post-coital cervical mucus was developed, with special attention to the moving spermatozoa. The test is performed in a square capillary tube (edge 0,8 mm). The migration rate, -- velocity and -- direction are measured by means of an ocular micrometer, which is seen projected over the capillary tube.
|
6ece9804-3f88-4d98-bd7b-e9bf5c600de2
| 0 |
I-cell disease (mucolipidosis II):a report on its pathology. The single most characteristic morphological feature in I-cell disease (ICD) is the accumulation of membrane-bound vacuoles in mesenchymal cells (mainly fibroblasts). No true storage can be documented in those vacuoles. That their contents could have been dissolved during fixation or embedding remains however a possibility.
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6ece9804-3f88-4d98-bd7b-e9bf5c600de2
| 1 |
Remnants consisting of a few lamellar arrays and of small amounts of fibrillo-granular material are too scarce for histochemical characterization. In hepatocytes large cells in the white pulp of the spleen and in myocardial fibers, vacuoles with fixative insoluble contents have been discovered; they are nowhere very abundant and their specificity is questionable.
|
6ece9804-3f88-4d98-bd7b-e9bf5c600de2
| 2 |
Because the affected fibroblastic elements represent a small fraction in any organ, most secondary biochemical abnormalities are expected to be detectable only in purely fibroblastic tissues. Our pathological study contributes to the understanding of some of the clinical features characteristic of ICD and stresses major morphological differences between ICD and the many diseases classified as mucopolysaccharidoses and mucolipidoses.
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c0707fbc-95f8-4e1b-9c83-f03f6f1abc01
| 0 |
Histochemical and morphological changes in human muscle spindle in upper and lower motor neuron lesions. The human intrafusal fibers were found to consist of two morphological and three histochemical (ATPase reaction) types. Two types of nuclear bag fibers were seen. Type A showed alkali stable and acid labile and Type B showed acid and alkali stable ATPase reaction.
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c0707fbc-95f8-4e1b-9c83-f03f6f1abc01
| 1 |
The nuclear chain fibers showed only alkali stable ATPase reaction. In the lower motor neuron atrophy, muscle spindle showed thickening of the capsule, atrophy of the nuclear chain fibers, and splitting of the nuclear bag fibers. ATPase reaction showed targetoid Type B nuclear bag fibers and 2 types of nuclear chain fibers.
|
c0707fbc-95f8-4e1b-9c83-f03f6f1abc01
| 2 |
Oxidative enzymes also showed targetoid nuclear bag and chain fibers. In upper motor neuron atrophy the spindle showed no changes.
|
c13329ad-7f0d-4917-bdd3-842323f2ec09
| 0 |
[Effect of an anxiolytic agent in hay fever]. In a group of 55 allergic patients with hay fever, and including patients treated by complete placebo, the action of Lorazepam has been studied from both the psycho-somatic and the allergic point of view. This most obviously allergic of all allergic diseases has been especially chosen because it was thought that the psycho-somatic hypothesis would appear at the outset in this condition to be the less apparent.
|
c13329ad-7f0d-4917-bdd3-842323f2ec09
| 1 |
Despite this fact, the anxiolitic effect of Lorazepam and its anti-allergic consequences were conclusive. A parallel study using only placebo treatment, orally and by vaccine, was performed in a few cases. 39 patients out of 45 treated by Temesta and placebo vaccine, had few or very few allergic symptoms.
|
c13329ad-7f0d-4917-bdd3-842323f2ec09
| 2 |
The 10 patients treated by complete placebo (orally and by vaccine) presented this year an acute pollinosis. We find the same parallelism between the allergy and the psycho-somatic aspect.
|
5d7ae9e5-5ec1-4175-aa99-4b7020413f39
| 0 |
A case of methylmalonic and propionic acidemia due to methulmalonyl-CoA carbonylmutase apoenzyme deficiency. A patient presenting with a deep metabolic acidosis after birth is described. Gas chromatographic analysis of short chain fatty acid and non volatile organic acids revealed the presence of both propionic and methylmalonic acid. In plasma obtained immediately after death the propionic- and methylmalonic acid concentrations were measured after separation of both acids by thin layer chromatography.
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5d7ae9e5-5ec1-4175-aa99-4b7020413f39
| 1 |
The propionic acid concentration was about 5 mM while the methylmalonic acid concentration was 2.6 mM. The methylmalonic acid concentration in urine was 6.8 mM. Propionyl-CoA carboxylase activity measured in leucocytes and liver-mitochondria revealed normal values (53 pmoles/min/mg protein and 6.5 nmoles/min/mg protein respectively).
|
5d7ae9e5-5ec1-4175-aa99-4b7020413f39
| 2 |
2-14C-Methyl-malonate oxydation in intact fibroblasts was totally blocked in the patient's cells. The methylmalonyl-CoA carbonyl mutase activity was found to be absent in the patient's fibroblasts. Addition of vit. B12 coenzyme to the incubation mixture stimulated 14C-succinate formation in the control cells but not in the patient's cells.
|
d7e9c5d6-1fc2-4d05-94c1-d5030ebf04c9
| 0 |
Enzyme histochemical studies of rabbit bile ducts with and without bile flow. The histochemical enzyme pattern in normal, extra-hepatic bile ducts from rabbits was studied. A difference between the duct epithelium and the crypt epithelium was noted mainly in the activity of gamma-glutamyl-transpeptidase and alkaline phosphatase which only stained positively in the crypts. No difference from the normal enzyme pattern was noted after diversion of the bile flow for up to 30 days.
|
44e64f55-fe8e-4a91-8bf3-9146deb9362c
| 0 |
Survival of anaerobic bacteria during transportation. 1. Experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen. The effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. Evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4
|
44e64f55-fe8e-4a91-8bf3-9146deb9362c
| 1 |
per cent. Three B. fragilis strains and one strain of Fusobacterium mortiferum and of Peptostreptococcus anaerobius were investigated. Bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and pure nitrogen, was compared to bacterial recovery from samples transported with free access to atmospheric air.
|
44e64f55-fe8e-4a91-8bf3-9146deb9362c
| 2 |
Evacuation by pure carbon dioxide significantly improved the recovery of one B. fragilis strain after 24 hours of transportation and significantly impaired the recovery of Peptostreptococcus anaerobius after one hour of transportation, while evacuation by pure nitrogen significantly improved the recovery of Peptostreptococcus anaerobius after 24 hours of transportation.
|
44e64f55-fe8e-4a91-8bf3-9146deb9362c
| 3 |
In all other cases, however, no statistically significant effect on bacterial recovery was found.
|
40b354b5-4602-4b94-8bee-b7a9ea2f293f
| 0 |
The spread of the action potential through the T-system in hagfish twitch muscle fibres. The input impedance of twitch muscle fibres of the Atlantic hagfish has been measured with sinusoidal transmembrane currents. The apparent specific membrane resistance and capacitance decreased markedly with frequency, and were relatively independent of fibre diameter.
|
40b354b5-4602-4b94-8bee-b7a9ea2f293f
| 1 |
A model of the T-system based on anatomical observations, was used to predict the input impedance in the normal solution (artificial sea water). The changes in input impedance produced by glycerol treatment, low chloride solution, reduced pH and isotonic solutions with low ionic strength were easily interpreted in terms of the same model.
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40b354b5-4602-4b94-8bee-b7a9ea2f293f
| 2 |
The model predicts severe attenuation of the action potential if conducted electrotonically by the transverse tubules towards the center of the fibre.
|
a44afa83-5e7e-49ee-b34c-86018c1fe47e
| 0 |
Withdrawal psychosis: a study of 30 consecutive cases. A study was carried out of 30 consecutive patients with withdrawal psychosis who in the period 1972 to 1975 were admitted to a psychiatric department or were attended by the department while hospitalized in a somatic department. There was a clear majority of women among cases of psychosis following drug withdrawal (15 as against four) and a clear majority of men among cases of psychosis following alcohol withdrawal (nine as against two).
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a44afa83-5e7e-49ee-b34c-86018c1fe47e
| 1 |
Competing pathogenetic factors could be considered present in most cases in the last mentioned group. In most cases the abrupt cessation took place in conjunction with admission to hospital, most frequently surgical cases or cases of acute drug toxication. In other cases abrupt cessation was decided upon by the patient himself.
|
a44afa83-5e7e-49ee-b34c-86018c1fe47e
| 2 |
Frequently predelirium treatment was either omitted or was given in the form of neuroleptics. Approximately a quarter of the patients initially denied their abuse. The study indicates that withdrawal psychosis can make its debut or become manifest at so late a stage as about the 14th day of the withdrawal phase following use of benzodiazepines and d-propoxiphene.
|
a44afa83-5e7e-49ee-b34c-86018c1fe47e
| 3 |
It is further indicated that abrupt cessation of benzodiazepines taken in "therapeutic" doses for several years in some instances can give rise to a withdrawal psychosis.
|
4d5cf888-74d9-48ba-b681-90b61734a7db
| 0 |
Nephroangiography in Wegener's granulumatosis. A comparison with panarteritis nodosa. Three cases of Wegener's granulomatosis with a classical course are described, 2 of which with fatal outcome in spite of immuno-suppressive therapy. Nephroangiography was performed during the oliguric or anuric phase. The appearances were similar to those encountered in glomerulonephritis and were compared with those in three cases of panarteritis nodosa.
|
4d5cf888-74d9-48ba-b681-90b61734a7db
| 1 |
Two of these represented the classical form with intrarenal arterial aneurysms; the third was a case of the microscopic type presenting blurred intrarenal arteries with lumen variations and occlusions. These observations support the opinion that Wegener's granulomatosis nodosa are different diseases. Nephroangiography seems to be of value in their differentiation.
|
3de3c705-71c6-4062-9e77-1ab8945348d4
| 0 |
The effect of metoprolol --a new selective adrenergic beta1-receptor blocking agent-- in mild hypertension. Metoprolol, a new selective adrenergic beta1-receptor blocking drug, has been compared to placebo in a series of 24 women who previously had taken alprenolol and propranolol during a cross-over study.
|
3de3c705-71c6-4062-9e77-1ab8945348d4
| 1 |
Blood pressure (BP) and heart rate (HR) were significantly reduced during metoprolol treatment compared to placebo. The occurrence of side-effects was similar during treatment with metoprolol and placebo. After the cross-over study the patients continued on metoprolol for more than two years, except for short periods on placebo or propranolol.
|
3de3c705-71c6-4062-9e77-1ab8945348d4
| 2 |
During this follow-up period BP remained at a similar level when the metoprolol dose was unchanged. Dose reduction (from 50 or 100 mg t.i.d. to 50 or 100 mg b.i.d.) caused no, or only a very slight, increase in BP. No side-effects were reported during long-term treatment.
|
f03edfa4-3965-4af3-8a99-8e3e75ddeccd
| 0 |
Comparison between metoprolol and propranolol as antihypertensive agents. A double-blind cross-over study. A new selective beta-adrenergic blocking agent--metoprolol--is compared to a non-selective beta-adrenergic blocking agent--propranolol--according to a double-blind cross-over technique in 23 hypertensive women, who had previously taken alprenolol and propranolol during different periods. No significant differences were found for blood pressure, heart rate, body weight or serum uric acid. No side-effects which could be related to the therapy were seen with either drug.
|
d048c9f3-303a-4b78-b464-b936cf8ec9d8
| 0 |
Effects of metabolic and pharmacologic interventions on myocardial infarct size following coronary occlusion. A number of hemodynamic, pharmacologic and metabolic interventions were found to change the extent of acute ischemic injury of the myocardium and subsequent necrosis following experimental coronary artery occlusion. Reduction in myocardial damage occurred by decreasing myocardial oxygen demands (beta-adrenergic blocking agents, intra-aortic balloon counterpulsation, external counterpulsation, nitroglycerin, decreasing afterload in hypertensive patients, inhibition of lipolysis, and digitalis in the failing heart);
|
d048c9f3-303a-4b78-b464-b936cf8ec9d8
| 1 |
by increasing myocardial oxygen supply either directly (coronary artery reperfusion or elevating arterial pO2), or through collateral vessels (elevation of coronary perfusion pressure by alpha-adrenergic agonists, intra-aortic balloon counterpulsation); or by increasing plasma osmolality (mannitol, hypertonic glucose); presumably by augmenting anaerobic metabolism (glucose-insulin-potassium, hypertonic glucose);
|
d048c9f3-303a-4b78-b464-b936cf8ec9d8
| 2 |
by enhancing transport to the ischemic zone of substrates utilized in energy production (hyaluronidase); by protecting against autolytic and heterolytic damage (hydrocortisone, cobra venom factor, aprotinin). Augmentation of myocardial ischemic damage occurred as a consequence of increasing myocardial oxygen requirements (isoproterenol, glucagon, ouabain, bretylium tosylate, tachycardia);
|
d048c9f3-303a-4b78-b464-b936cf8ec9d8
| 3 |
by decreasing myocardial oxygen supply either directly (hypoxia, anemia) or through reduction of collateral flow (hemorrhagic hypotension, minoxidil) or by decreasing substrate availability glycemia). Pilot studies have been carried out in patients with hyaluronidase, nitroglycerin, intra-aortic balloon counterpulsation, beta-blocking agents and Arfonad and have shown that these interventions may also reduce myocardial damage, suggesting that the concept of reduction in infarct size following coronary occlusion is applicable clinically.
|
4bc6ad7f-99a8-4bc2-9b98-2bf6cc6c65a4
| 0 |
Factors of importance for the degree of ischemic injury in the isolated rat heart. Isolated working rat hearts were made ischemic by introducing a one-way aortic ball valve. After the ischemic period the hearts were perfused in a retrograde non-working way for 30 min. Flow rates, glycogen, ATP, and creatine-phosphate went down during the time of ischemia, whereas tissue lactate accumulated.
|
4bc6ad7f-99a8-4bc2-9b98-2bf6cc6c65a4
| 1 |
For shorter periods of ischemia these values were normalized but after 30 min of ischemia the hearts seemed to be irreversibly damaged. There was a leakage of GOT, GPT, LDH, and CPK from all hearts when ischemic from 5 to 30 min. Different factors that might be of importance for the degree of ischemic injury were tested.
|
4bc6ad7f-99a8-4bc2-9b98-2bf6cc6c65a4
| 2 |
The injury tended to be more severe at higher heart rates. Addition of adrenaline 10(-6)M resulted in excessive myocardial damage. A variation of pH from 7.1 to 7.7 did not alter the effects of the ischemic injury. One group of rats were injected with adrenaline for 8 weeks to simulate chronic stress.
|
4bc6ad7f-99a8-4bc2-9b98-2bf6cc6c65a4
| 3 |
When hearts from these rats were made ischemic they were more prone to fail compared to controls. The failing hearts, on the other hand, had a lower leakage of enzymes, possibly due to a less severe myocardial damage. A high mechanical performance and a normal noradrenaline content of the hearts are key factors for the development of myocardial infarction, as indicated by this study.
|
d96b4f82-a774-43eb-8632-dfc756c38dc9
| 0 |
Double-blind study of the effect of cardioselective beta-blockade on chest pain in acute myocardial infarction. A double-blind study including three different cardioselective beta-blockers, practolol, H 87/07 and metoprolol, was performed in 54 patients with acute myocardial infarction and chest pain shortly after onset of symptoms.
|
d96b4f82-a774-43eb-8632-dfc756c38dc9
| 1 |
Transmural infarctions were found in 42 patients while 12 patients had nontransmural infarctions. Chest pain and the product of heart rate and systolic blood pressure were significantly reduced in the beta-blocker groups whereas no changes were seen after saline. All patients with nontransmural infarctions and 14 out of 29 with transmural infarctions got pain relief lasting for at least 30 min.
|
d96b4f82-a774-43eb-8632-dfc756c38dc9
| 2 |
None of the patients developed signs of left ventricular backward failure, shock, or bradycardia. A decrease in ST segment elevation was observed in all the transmural infarctions after beta-blockade. No changes in ST segment elevation were found after analgesics when given after saline, but in some cases an increase was seen in this parameter when analgesics were given due to insufficient pain relief after beta-blockers or due to return of chest pain.
|
d96b4f82-a774-43eb-8632-dfc756c38dc9
| 3 |
It is suggested that pain relief by beta-blockers indicates decrease of myocardial ischemia.
|
5d017a0d-3f21-49e7-95ed-1e4e17f6b3f8
| 0 |
Multiple endocrine adenomatosis syndromes. MEA I and II are two genetically distinct tumor endocrinopathies, both showing autosomal dominant inheritance. Little overlap exists between these conditions, and that which is present can be explained on the basis of two mutually exclusive factors: (1) the secondary consequences of hormone excess on another endocrine gland or (2) the fact that both tumor syndromes appear to result from genetically faulty differentiation of neuroectoderm.
|
5d017a0d-3f21-49e7-95ed-1e4e17f6b3f8
| 1 |
A seemingly disproportionate amount of effort has been expended on study of the MEA syndromes. However, there would seen to be ample justification for this interest: 1) The MEA syndromes, unlike most neoplastic conditions, are hereditary and can be readily detected and more expeditiously treated; 2) hormone radioimmunoassay has greatly facilitated diagnosis in asymptomatic individuals;
|
5d017a0d-3f21-49e7-95ed-1e4e17f6b3f8
| 2 |
and (3) probably most importantly, study of these syndromes has provided considerable insight into the embryologic origin of the endocrine system. It is conceivable that knowledge gained from these conditions may stimulate further inquiry into the processes whereby neoplasia occurs in endocrine tissue and thus lead the way to the development of effective therapy for a host of hormone-producing tumors.
|
1ef6a392-bb35-49f2-b62f-87c80d650121
| 0 |
Folic acid absorption in regional enteritis. The authors have investigated intestinal absorption of folic acid by jejunal perfusion with a triple lumen tube in five subjects with regional enteritis. The subjects' intestinal disabilities ranged from terminal ileitis to short bowel syndrome. Two had steatorrhea and two had low serum folate levels. Absorption of pteroylglutamic acid was normal in all five. This suggests that folic acid deficiency, common in this disorder, is largely caused by malnutrition, not malabsorption.
|
a84ee103-affd-4fe2-9cc6-d5e5b6e416b8
| 0 |
Acid, neutral, and alkaline hydrolases in arthritic synovium. The levels of six lysosomal enzymes (acid phosphatase, beta-acetylglucosaminidase, cathepsin D, beta-galactosidase, arylsulfatase A, and beta-glucuronidase) and four neutral and alkaline hydrolases (esterase, inorganic phyrophosphatase, alkaline phosphatase, and 5'-nucleotidase) were measured in osteoarthritic, rheumatoid and control synovia.
|
a84ee103-affd-4fe2-9cc6-d5e5b6e416b8
| 1 |
All enzyme levels in diseased synovium except esterase values in osteoarthritis were significantly elevated compared with controls. The mean values of the group of acid hydrolases and the group of neutral and alkaline hydrolases in osteoarthritic synovia were 1.9- and 2.0-fold greater than those of control specimens. In rheumatoid synovia, the values were 4.2
|
a84ee103-affd-4fe2-9cc6-d5e5b6e416b8
| 2 |
- and 4.5 fold greater than control for the same enzymes. Levels in rheumatoid synovia were significantly higher than those in osteoarthritic synovia with the exception of 5'-nucleotidase. Only a limited correlation between the extents of inflammation present in the synovia and the levels of a lysosomal marker enzyme (cathepsin D) was observed.
|
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