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3342_biomrc
|
Title: XXXX at high surgical risk: the endovascular approach.
AIMS: AAS, Acute aortic syndromes ( AAS, Acute aortic syndromes ) still represent life-threatening conditions. The aim of this study was to describe our experience in the management of patients, patient (pts) with AAS, Acute aortic syndromes and to evaluate the safety and feasibility of EVT, endovascular treatment ( EVT, endovascular treatment ) in high surgical risk patients, patient . METHODS AND RESULTS: One hundred and four patients, patient underwent EVT, endovascular treatment . We selected 56 pts with AAS, Acute aortic syndromes : 17 complicated type B aortic dissections, five traumatic patients, patient755 at the isthmus, 11 thoracic aneurysms and 23 pts with large AAA with impending rupture . All these pts were at high surgical risk because of their comorbidities and/or their emergency situation. They were clinically followed during hospitalisation and they underwent a 2 mm-interval CT-scan two weeks, six and 12 months after discharge and every year after. Death , paraplegia , open surgical conversion did not occur. Two pts underwent a successful secondary EVT, endovascular treatment for patients, patient070 . One patients, patient with thoracic aortic aneurysm died of septic shock from pneumonia 78 days after discharge and two pts with AAA suffering from a severe three-vessel coronary disease experienced sudden death at one year follow-up. CONCLUSIONS: EVT, endovascular treatment seems to be a safe and effective therapeutic option with good short- and midterm results in patients, patient with AAS, Acute aortic syndromes at high surgical risk. Thus, it can be considered as a less-invasive alternative in patients, patient considered otherwise unsuitable for conventional surgery, even though a careful, continued follow-up is still necessary to confirm the long-term safety and effectiveness of EVT, endovascular treatment in AAS, Acute aortic syndromes .
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1297_biomrc
|
Title: Homology modelling and site-directed mutagenesis studies of the XXXX hydrolase from Phanerochaete chrysosporium.
Three-dimensional structural model of epoxide hydrolase (PchEHA) from Phanerochaete chrysosporium was constructed based on X-ray structure of Agrobacterium radiobacter AD1 sEH using SWISS-MODEL server. Conserved residues constituting the active site cavity were identified, of which the functional roles of 14 residues were determined by site-directed mutagenesis. In catalytic triad, Asp 105 and His308 play a leading role in alkylation and hydrolysis steps, respectively. Distance between Asp 105 and epoxide ring of substrate may determine the regiospecificity in the substrate docking model. Asp 277 located at the entrance of substrate tunnel is concerned with catalysis but not essential. D307E had the highest activity and lower enantioselectivity among 14 mutants, suggesting Asp 307 may be involved in choice of substrate configuration. Y159F and Y241F almost exhibited no activity, indicating that they are essential to bind substrate and facilitate opening of epoxide ring. Besides, His35- Gly 36- Asn 37-Pro38, Trp106 and Trp309 surrounding Asp 105, may coordinate the integration of active site cavity and influence substrate binding. Especially, W106I reversed the enantioselectivity, perhaps due to more deteriorative impact on the preferred (R)-styrene oxide . Gly 65 and Gly67 occurring at b-turns and Gly36 are vital in holding protein conformation. Conclusively, single conserved residue around the active sites has an important impact on catalytic properties.
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1302_biomrc
|
Title: Sensitivity of clinical and behavioural tests of XXXX after right hemisphere @entity64 .
OBJECTIVES: The lack of agreement regarding assessment methods is responsible for the variability in the reported rate of occurrence of Patients, patients057 after stroke . The aim of this study was to assess the sensitivity of different tests of neglect after right hemisphere stroke . METHODS: Two hundred and six subacute right hemisphere stroke Patients, patients were given a test battery including a preliminary assessment of Patients, patients054 and of visual extinction, a clinical assessment of gaze orientation and of personal neglect, and paper and pencil tests of Patients, patients057 in the peripersonal space. Patients, patients were compared with a previously reported control group. A subgroup of Patients, patients (n=69) received a behavioural assessment of neglect in daily life situations. RESULTS: The most sensitive paper and pencil measure was the starting point in the cancellation task. The whole battery was more sensitive than any single test alone. About 85% of Patients, patients presented some degree of neglect on at least one measure. An important finding was that behavioural assessment of neglect in daily life was more sensitive than any other single measure of neglect. Behavioural neglect was considered as moderate to severe in 36% of cases. A factorial analysis revealed that paper and pencil tests were related to two underlying factors. Dissociations were found between extrapersonal neglect, personal neglect, Patients, patients054 , and extinction. Anatomical analyses showed that neglect was more common and severe when the posterior association cortex was damaged. CONCLUSIONS: The automatic rightward orientation bias is the most sensitive clinical measure of neglect. Behavioural assessment is more sensitive than any single paper and pencil test. The results also support the assumption that neglect is a heterogeneous disorder .
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1303_biomrc
|
Title: Patch Testing for @entity16880 and @entity16881 - XXXX Contact Allergy.
Importance: Contact allergy, allergies to patients, patient, Participants6880 (MI) or to the combined formulation of patients, patient, Participants6881 and MI (MCI-MI) has increased significantly, with a frequency of as much as 11.1% in patients, patient, Participants with patients, patient, Participants909 ; however, few cohort studies in the US population have been reported. Objective: To investigate the prevalence of contact allergy, allergies to MI and MCI-MI and the outcomes of patients, patient, Participants with positive patch test reactions to MI and MCI-MI. Design, Setting, and patients, patient, Participants : Retrospective medical record review of all consecutive patients, patient, Participants (n = 703) presenting with possible allergic contact dermatitis and subsequently undergoing patch testing at a tertiary referral center from January 1, 2012, through November 30, 2014. Patch testing used the North American Contact Dermatitis Group standard series. The MCI-MI concentration was 100 ppm; the MI concentration in the screening series increased from 200 to 2000 ppm in January 2013. Demographic data, exposures, and outcomes were reported for patients, patient, Participants with positive patch test reactions to MI and/or MCI-MI. Data were analyzed from December 1, 2014, through January 31, 2015. Main Outcomes and Measures: Positive patch test reaction to MI and/or MCI-MI and identification of the relevance of contact allergy, allergies to these preservatives. Follow-up after allergen avoidance was determined when available. Results: Of the 703 patients, patient, Participants who underwent patch testing, contact allergy, allergies to MI and/or MCI-MI occurred in 57 patients, patient, Participants (8.1%), with 35 reactions to MI only, 5 reactions to MCI-MI only, and 17 reactions to both. Prevalence of contact allergy, allergies to MI increased from 6 of 236 patients, patient, Participants (2.5%) in 2012 to 16 of 235 patients, patient, Participants (6.8%) in 2014. The most commonly affected sites were the hands and face. Contact allergy, allergies to MI and/or MCI-MI was occupationally related in 4 cases. Cosmetics, soaps and cleansers (including wet wipes), and hair care products accounted for all identified sources. Twenty-three patients, patient, Participants had follow-up data, and 17 of these (74%) improved with allergen avoidance. Conclusions and Relevance: The increasing prevalence of contact allergy, allergies to MI alone supports its addition to the standard series to identify cases missed by testing only for MCI-MI. Recent regulations by the European Commission have banned MCI-MI in all leave-on body products as of July 16, 2015. Currently recommended US regulations are less stringent; however, US regulatory agencies must act to ensure patients, patient, Participants safety.
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3351_biomrc
|
Title: Concomitant chemoradiotherapy with low-dose weekly XXXX for nonmetastatic unresectable @entity788 .
BACKGROUND/AIMS: This study aimed to demonstrate the efficacy and tolerability of low-dose weekly Patients, patients, patient36 as a radiosensitizer in unresectable pancreatic cancer Patients, patients, patient treated with chemoradiotherapy. METHODS: Twenty-four histologically confirmed pancreatic carcinoma Patients, patients, patient (female/male: 10/14, median age: 60) were evaluated. Seven (29%) Patients, patients, patient received Patients, patients, patient36 either as a single agent or in combination prior to chemoradiotherapy. Concurrent 75 mg/m2 Patients, patients, patient36 was infused weekly. Radiotherapy was delivered to the primary tumor and positive lymphatics with 3D-conformal radiotherapy to a total dose of 4500 cGy. Local progression-free survival, distant metastasis-free survival and overall survival were evaluated by Kaplan-Meier method. RESULTS: Median follow-up was 36 weeks. Median local progression-free survival, distant metastasis-free survival and overall survival were 22 weeks (95% confidence interval [CI]: 5-59 weeks), 19 weeks (95%CI: 6.9-31 weeks) and 36 weeks (95%CI: 28-43 weeks), respectively. All Patients, patients, patient completed radiotherapy as scheduled. Concurrent Patients, patients, patient36 was given fully in 58.3% of Patients, patients, patient . Patients, patients, patient36 was terminated in four (16.6%) Patients, patients, patient due to grade 3 Patients, patients, patient398 (n=1), grade 3 nausea / vomiting (n=2) or Patients, patients, patient 's reluctance (n=1). Patients, patients, patient with local response and stable disease to chemoradiotherapy revealed a median survival of 39 weeks (95%CI: 30-47.9 weeks) compared to 36 weeks (95%CI: 9.7-62.2 weeks) in Patients, patients, patient with locally progressive disease (p=0.52). Patients, patients, patient58 was improved in 50% of Patients, patients, patient . CONCLUSIONS: Weekly low-dose radiosensitizing Patients, patients, patient36 is effective and safe in unresectable pancreatic cancer Patients, patients, patient .
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1304_biomrc
|
Title: Dysfunction of XXXX cerebral arteries during early aging.
UNASSIGNED: Aging leads to a gradual decline in the fidelity of cerebral blood flow (CBF) responses to neuronal activation, resulting in an increased risk for stroke and dementia . However, it is currently unknown when age-related cerebrovascular dysfunction starts or which vascular components and functions are first affected. The aim of this study was to examine the function of microcirculation throughout aging in mice, N mice . Microcirculation was challenged by inhalation of 5% and 10% CO2 or by forepaw stimulation in 6-week, 8-month, and 12-month-old FVB/ mice, N mice . The resulting dilation of pial vessels and increase in CBF was measured by intravital fluorescence microscopy and laser Doppler fluxmetry, respectively. Neurovascular coupling and astrocytic endfoot Ca(2+) were measured in acute brain slices from 18-month-old mice, N mice . We did not reveal any changes in CBF after CO2 reactivity up to an age of 12 months. However, direct visualization of pial vessels by in vivo microscopy showed a significant, age-dependent loss of CO2 reactivity starting at 8 months of age. At the same age neurovascular coupling was also significantly affected. These results suggest that aging does not affect cerebral vessel function simultaneously, but starts in pial microvessels months before global changes in CBF are detectable.Journal of Cerebral Blood Flow _ Metabolism advance online publication, 10 June 2015; doi:10.1038/jcbfm.2015.107.
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3354_biomrc
|
Title: [Posterior fossa subdural @entity1410 in the newborn caused by XXXX ].
Among various cases of intracranial hemorrhage in the newborn caused by birth injury , posterior fossa subdural patient, infant410 is of serious nature in many cases and often results in death after critical clinical course due to compression of the respiratory center of the medulla oblongata. We have recently experienced two cases of subdural patient, infant410 in the posterior fossa caused by birth injury , which we successfully treated non-surgically. Herein, we report these two cases and present a sequential CT scan of each. The first case is a full-term (39 weeks gestation) male patient, infant . Delivery was carried out spontaneously with double footling presentation. At 20 hours of age, cyanosis and convulsion occurred. CT scan was performed and revealed high density areas in the posterior fossa, quadrigeminal cistern and longitudinal cerebral fissure. Bloody CSF was discharged per lumbar puncture and glycerol was infused intravenously, but the ventricle became enlarged. At this point at 9 days of age, the patient, infant was admitted to our hospital. While he showed poor activity on admission, patient, infant410 was absorbed gradually and disappeared at 3 months of age by conservative treatment. Now, at 6 years and 6 months of age, the patient, infant has no neurological deficits . The second case is a full-term (40 weeks gestation) twin female patient, infant . Due to breech presentation, delivery was conducted per breech extraction. At three days of age, vomiting , fontanel bulging and hypotonia were observed. CT scan revealed patient, infant410 similar to that seen in the first case, and patient, infant34 and glycerol were infused intravenously. Conservative treatment was performed in this case also, and changes shown by CT scan were almost the same as those seen in the first case. The patient, infant has no neurological deficits at 5 years and 7 months of age. Since the introduction of CT scan, early diagnosis of a subdural patient, infant410 in the posterior fossa has been possible. But reports of successful surgical treatment are not so frequent.(ABSTRACT TRUNCATED AT 250 WORDS)
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1310_biomrc
|
Title: Hierarchical paracrine interaction of XXXX associated fibroblasts with @entity5 cells via hMAPK-microRNAs to drive ER-negative @entity0 phenotype.
UNASSIGNED: Multiple juxtacrine and paracrine interactions occur between tumor, cancer, Cancer cells and non- tumor, cancer, Cancer cells of the tumor, cancer, Cancer microenvironment (TME) that direct tumor, cancer, Cancer progression. tumor, cancer, Cancer Associated Fibroblasts (CAFs) are an integral component of the TME, and the majority of breast cancers, breast cancer, breast tumors, breast tumor stroma is comprised of CAFs. Heterotypic interactions between tumor, cancer, Cancer cells and non- tumor, cancer, Cancer cells of the TME occur via soluble agents, including cytokines, hormones, growth factors, and secreted microRNAs. We previously identified a microRNA signature indicative of hyperactive MAPK signaling (hMAPK-miRNA signature) that significantly associated with reduced recurrence-free and overall survival. Here we report that the hMAPK-miRNA signature associates with a high metric of stromal cell infiltrate, and we investigate the role of microRNAs, particularly hMAPK-microRNAs, secreted by CAFs on estrogen receptor (ER) expression in breast cancers, breast cancer, breast tumors, breast tumor cells. ER-positive MCF-7/ltE2- cells were treated with CM, conditioned media ( CM, conditioned media ) from CAFs derived from breast cancers, breast cancer, breast tumors, breast tumor of different PAM50 subtypes (CAFBAS, CAFHER2, and CAFLA). CAF CM, conditioned media isolated specifically from ER-negative primary breast cancers, breast cancer, breast tumors, breast tumor led to ER repression in vitro. Nanoparticle tracking analysis and transmission electron microscopy confirmed the presence of CAF-secreted exosomes in CM, conditioned media and the uptake of these exosomes by the ER+ MCF-7/ltE2- cells. Differentially expressed microRNAs in CAF CM, conditioned media as well as in MCF-7/ltE2- cells treated with this CM, conditioned media were identified. Knockdown of miR-221, miR221 /222 in CAFBAS resulted in knockdown of miR-221, miR221 /222 levels in the CM, conditioned media and the CM, conditioned media from CAFBAS; miR-221, miR221 /222 knockdown rescued ER repression in ER-positive cell lines treated with CAFBAS- CM, conditioned media . Collectively, our results demonstrate that CAF-secreted microRNAs are directly involved in ER-repression, and may contribute to the MAPK-induced ER repression in breast cancers, breast cancer, breast tumors, breast tumor cells.
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3359_biomrc
|
Title: Transformation of the estrogen-receptor complex from XXXX oviduct in 2 steps.
Salt treatment of the cytoplasmic estradiol -receptor complex from chick oviduct induces a strong affinity of the complex for DNA-cellulose and phenyl-sepharose . This process is called activation. Binding to heparin- and lysozyme-sepharose is also observed with the untreated complex. But, the salt treatment, additional binding of the complex to these adsorbents is seen. The increased ability of the complex to bind to polyanions and polycations is destroyed by mild trypsination. The binding to the hydrophobic adsorbent is not affected by this treatment. Neither a change of the sedimentation constant nor of the size of the receptor protein is observed after salt treatment in the cold. After binding of the salt-activated estradiol -receptor complex to DNA-cellulose in the cold, an increase of its sedimentation constant and its size, as measured by density-gradient centrifugation and agarose gel chromatography, resp., becomes apparent. A similar phenomenon is observed after binding to DEAE-cellulose and to some extent after binding to heparin-sepharose. The nuclear complex seems to have the same sedimentation constant as the cytoplasmic complex eluted from DNA-cellulose. The sedimentation constant of the nuclear complex is not changed after DNA-cellulose chromatography. The cytoplasmic progesterone -receptor complex from the same tissue, i.e. the oviduct, does not show any change of size. Thus the well-known process of transformation can now be separated into 2 steps. (1) Activation of the estradiol -receptor complex for its binding to various adsorbents in vitro and probably to its acceptor site(s) in vivo. (2) Increase of receptor size. This second step seems to be a special property of the estradiol -receptor complex. Its physiological significance is unclear.
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1311_biomrc
|
Title: [Dynamic assessment of corpus callosum and corticospinal tracts structure using diffusion-tensor XXXX in @entity21 ].
BACKGROUND: DAI, Diffuse axonal injury ( DAI, Diffuse axonal injury ) causes neurodegenerative processes in brain which can last weeks and months after patients31 . Aim of this study was to assess structural changes of corpus callosum and corticospinal tracts in dynamics using diffusion-tensor magnetic resonance imaging (DT- MRI ) in severe DAI, Diffuse axonal injury . MATERIALS AND METHODS: 14 patients with severe DAI, Diffuse axonal injury (GCS < or = 8 in acute period) were examined using DT- MRI . In 12 cases 1.5 Tesla device was used, in 2-3 Tesla tomography was applied. Initial studies were performed on 3rd-17th days after injury and control studies were done between 3 weeks and 33 months after injury. Outcomes were assessed using GOS 6 months after injury and later. RESULTS: MR-tractography demonstrated almost absolutely absent visualization of ascending fibers of corpus callosum 3-20 weeks after patients545 in 5 patients with poor outcomes ( severe disability and persistent vegetative state). Raized asymmetry of corticospinal tracts was associated with patients269 or patients688 in the same patients . In 6 patients with severe disability partial loss and thinning of corpus callosum fibers were observed. In 2 patients with good recovery and moderate disability repeated studies showed no severe changes in structure of corpus callosum. CONCLUSION: DT- MRI presents new data about structural changes of white matter tracts in patients31 .
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3365_biomrc
|
Title: Contrasting effects of subtotal enteric bypass, enterectomy, and colectomy on @entity2985 -induced intestinal XXXX .
Compensatory hyperplasia, adaptive hyperplasia after extensive loss of functioning small or large intestine might predispose to the development of Anastomotic tumors, neoplasia, tumors in the residual adapted bowel. To test this hypothesis, male Fischer rats were randomized to receive 85 to 90% jejunoileal resection or bypass, subtotal colectomy, or no operation (controls). One week later, the first of six weekly s.c. injections of azoxymethane (15 mg/kg/week) was given. At the 36th week postoperatively, mean body weight after enterectomy or colectomy it was 78 to 79% of control. Adaptation after all three operations was characterized by 22 to 84% increments in villous height and crypt depth in the residual functioning ileum (p = 0.05 to 0.001); the depth of colonic crypts was unchanged. Fewer rats developed intestinal tumors after enteric bypass (36%) than after any of the other treatments (80 to 91%) (p = 0.01 to 0.001); the depth of colonic crypts was unchanged. Fewer rats developed intestinal tumors after enteric bypass (36%) than after any of the other treatments (80 to 91%) (p = 0.01 to 0.001). Compared with controls, bypass reduced the number of colonic tumor, colonic tumors by 77% (p less than 0.001). Although resection did not affect colonic tumor, colonic tumors yield, it tripled the incidence of Anastomotic tumors, neoplasia, tumors in the duodenum and jejunum (p = 0.025). Colectomy promoted carcinogenesis, rectal carcinogenesis (p less than 0.05). Anastomotic tumors, neoplasia, tumors were commoner after intestinal resection. the lower frequency of Anastomotic tumors, neoplasia, tumors after jejunoileal bypass contrasts with enhanced carcinogenesis, rectal carcinogenesis after enterectomy or colectomy. Profound reduction in body weight may prevent the promotional effect of Compensatory hyperplasia, adaptive hyperplasia .
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1317_biomrc
|
Title: XXXX to the breast from non-mammary @entity5 : @entity5 , prevalence, clinical signs, and radiological features.
RATIONALE AND OBJECTIVES: Most secondary intramammary tumors, malignancies occur as metastatic involvement from the contralateral breast. Breast metastases (BM) from nonmammary tumors, malignancies are very rare. The aims of this study were to estimate retrospectively the prevalence of BM from nonmammary tumors, malignancies and to describe their radiologic appearance. MATERIALS AND METHODS: BM were identified in 51 women, patients, men , including 43 women, patients, men and eight women, patients, men with a median age of 61 years (range, 24-84 years). Computed tomography of the thoracic region identified 108 lesions in 38 women, patients, men . Mammography was available for 37 women, patients, men (54 lesions). Ultrasound evaluation was performed in 43 women, patients, men (71 lesions). In 24 women, patients, men (93 lesions), magnetic resonance imaging of the breast was done. Images were reviewed in consensus by two radiologists according to the Breast Imaging Reporting and Data System lexicon. RESULTS: The prevalence of BM in several tumors, malignancies ranged from 0.12% to 4.92%. On computed tomography, most metastases were round or oval in shape with marked or moderate enhancement. On mammography, solitary or multiple round or oval masses with circumscribed margins were the most common pattern of BM. Ten percent showed microcalcifications. On ultrasound, most BM were hypoechoic, oval or round in shape, with microlobulated or circumscribed margins, and posterior acoustic enhancement. Doppler imaging showed hypervascularity in 39% of BM. On magnetic resonance imaging, most lesions demonstrated marked homogenous contrast enhancement. Type 1 kinetic curve was seen in 18%, type 2 in 52%, and type 3 in 30%. CONCLUSIONS: The radiologic features reported in this study should be taken into consideration in the differential diagnosis of women, patients, men558 .
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3369_biomrc
|
Title: Diet and XXXX and rectum: a case-control study.
In 1979-81, 419 women, patients with incident cases of colon and rectal cancer, colon cancer and 732 controls were questioned regarding diet and women, patients67 . cancer, Cancer, rectal cancer cases were a population-based series reported to the South Australian Central cancer, Cancer, rectal cancer Registry, were 30-74 years of age, and were residing in Metropolitan Adelaide. Controls were selected from the electoral roll and individually age- and sex-matched to cancer, Cancer, rectal cancer cases. The most consistent risk factor for women, patients4 was dietary protein, which was associated with a twofold-to-threefold relative risk for colon and rectal cancer, colon cancer and for cancer, Cancer, rectal cancer in women, patients for all levels of consumption above the base line (i.e., the lowest consumption quintile). For male colon and rectal cancer, colon cancer the corresponding relative risk was similar; but for male cancer, Cancer, rectal cancer , risk was elevated only at old ages. Total energy intake and, less clearly, meal frequency were also positively associated with increased risk. Total women, patients67 intake (but not specifically beer) was associated with increased risk of both colon and rectal cancer, colon cancer in women, patients ; in both sexes, there was an increased risk of colon and rectal cancer, colon cancer associated with spirits consumption. A reduced risk of cancer, Cancer, rectal cancer was associated with vitamin C but not with vitamin A . The increased risk associated with high protein and total energy was confined to those consuming a low fiber diet, particularly among women, patients ; but some other aspects of the relationship between fiber consumption and risk of women, patients4 were more complex. Some modifications and extensions of the current fat-to- bile acid -to-fiber theory of bowel women, patients05 were suggested.
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1323_biomrc
|
Title: @entity9695 -loaded nanoparticles for the potential coadjuvant treatment of XXXX .
UNASSIGNED: Nimesulide (NS)-loaded nanoparticles (NPNS) were prepared from polylactide-co-glycolide ( PLGA ) and eventually coated with chitosan (NPNSCS). Nanoparticles (NP) were spherical with sizes 379 59nm for NPNS and 393 66nm for NPNSCS and zeta potentials of -15 3mV for NPNS to 10 4mV for NPNSCS, suggesting an efficient coating. Drug encapsulation rate was high (88 5% and 83 7% of added drug) for NPNS and NPNSCS, respectively. After NP washing and re-suspension, 98 2% and 99 1% of the drug initially entrapped remained associated to NP. NS was dispersed in amorphous state within the polymeric matrix. Two-fold dilution of NP with pH 7.4 PBS provoked no drug release. However, 30-40% NS was released after a 1/10 dilution. NPNSCS and NPNS diluted 1/100 reduced the encapsulated drug to around 30% and 70%, respectively. In contrast, 100% NS was released from NP under sink conditions in less than 2h. The permeability of free-NS (1-1.5*10(-5)cm/s) was compared with NPNS (NPNS=6.4-8.1*10(-6)cm/s and NPNSCS=5.5-7.0*10(-6)cm/s) using the PAMPA assay. The cytotoxicity of free-NS and NS in NP on model prostate cancer cells PC-3 and DU-145 showed the highest cytotoxic effect with NPNSCS on PC-3 cells (IC50=89 M).
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3372_biomrc
|
Title: Enhancement of quiet sleep in XXXX neonates by @entity7191 .
Muramyl peptides that induce excess slow-wave sleep have been isolated from urine and brain. A synthetic analogue to those substances, muramyl dipeptide, N-acetylmuramyl-L-alanyl-D-isoglutamine ( MDP , muramyl dipeptide, N-acetylmuramyl-L-alanyl-D-isoglutamine ), was found to induce prolonged increases in slow-wave sleep and to increase electroencephalographic (EEG) delta-wave activity. MDP is also pyrogenic, although this activity can be separated from its somnogenic activity. To further investigate the somnogenic actions of MDP , neonatal rabbits were used in the present study. Intraperitoneal injection of 100 micrograms/kg MDP induced differential somnogenic and pyrogenic effects; from postnatal days 7-9, MDP increased duration of quiet sleep (QS, the precursor of adult slow-wave sleep) and AS, decreased active sleep ( AS, decreased active sleep ) as judged by behavioral criteria. These animals were not febrile during the period of enhanced QS, nor did MDP alter EEG delta-wave activity at this age. From postnatal days 10-15, MDP induced prolonged (6 h) increases in duration of QS; both behavioral and EEG criteria were used at this age to determine duration of QS and AS, decreased active sleep . Maximum MDP -induced effects occurred during the 2nd h, with a parallel increase in amplitudes of EEG delta-wave activity. At this age, MDP also elicited monophasic fevers and inhibition of AS, decreased active sleep , with maximum effects observed during hours 3-4 postinjection. After postnatal day 16, MDP -induced somnogenic and febrile responses were similar to those observed in adult rabbits . We conclude that the mechanisms responsible for behavioral sleep states are responsive to a sleep-promoting substance early in ontogenesis.
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3375_biomrc
|
Title: Urmylation: a XXXX -like pathway that functions during @entity851 and budding in @entity375 .
ubiquitin, Ubiquitin is a small modifier protein that is conjugated to substrates to target them for degradation. Recently, a surprising number of ubiquitin, Ubiquitin -like proteins have been identified that also can be attached to proteins. Herein, we identify two molecular functions for the posttranslational protein modifier from Saccharomyces cerevisiae , Urm1p . Simultaneous loss of Urm1p and Cla4p , a p21-activated kinase that functions in budding, is lethal. This result suggests a role for the urmylation pathway in budding. Furthermore, loss of the urmylation pathway causes defects in invasive growth and confers sensitivity to rapamycin . Our results indicate that the sensitivity to rapamycin is due to a genetic interaction with the TOR pathway, which is important for regulation of cell growth in response to nutrients. We have found that Urm1p can be attached to a number of proteins. Loss of five genes that are also essential in a cla4Delta strain, NCS2 , NCS6 , ELP2 , ELP6 , and URE2 , affect the level of at least one Urm1p conjugate. Moreover, these five genes have a role in invasive growth and display genetic interactions with the TOR pathway. In summary, our results suggest the urmylation pathway is involved in nutrient sensing and budding.
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3377_biomrc
|
Title: @entity73 in adults with XXXX : The relationship between subjective and objective assessments of cognition.
AIM: This study aimed to assess the relationship between objective measures of cognition and subjective perception of cognitive functioning reported by Patients, patients, Patient with epilepsy and their caregivers. METHODS: One hundred Patients, patients, Patient with epilepsy attending hospital neurology outpatient clinics and their caregivers were enrolled in this study. The EpiTrack (version 1) brief cognitive screening tool was used to measure objective impairment, the ABNAS questionnaire (A-B Neuropsychological Assessment Schedule) to assess subjective cognitive performance, and a version of the ABNAS designed to be completed by caregivers (C-ABNAS) to document caregivers' views. Patients, patients, Patient Patients, patients, Patient48 and Depression, depression were measured using the Hospital Anxiety and Depression, depression Scale (HADS) and considered as covariates. Patients, patients, Patient with an uncertain diagnosis of epilepsy or likely severe comorbid mood or Patients, patients, Patient48 were excluded. RESULTS: Data from 82 Patients, patients, Patient were analyzed after exclusion of Patients, patients, Patient with uncertain diagnoses or likely severe comorbid mood or Patients, patients, Patient48 . Fifty-nine (72%) had a degree of objective cognitive impairment . Fifty (84.7%) of these 59 Patients, patients, Patient had 'high' ABNAS scores concordant with the objective assessment, and 43 (72.9%) had high C-ABNAS scores matching the abnormalities detected by objective screening. Of the 23 (28%) Patients, patients, Patient without objective cognitive impairment , seven (30.4%) had concordantly low ABNAS scores, and 10 (43.4%) had concordantly low C-ABNAS scores. Patients, patients, Patient memory dysfunction, memory impairment was more often reported by Patients, patients, Patient themselves than by caregivers (p=0.011). Carers were significantly more likely to rate Patients, patients, Patient as having impaired motor coordination than Patients, patients, Patient themselves. A small part of the variance of the EpiTrack score was predicted by the C-ABNAS. Objective cognitive performance did not predict ABNAS or C-ABNAS scores. CONCLUSIONS: Self-report or caregiver report questionnaires identify Patients, patients, Patient with epilepsy and objective cognitive impairment more accurately than Patients, patients, Patient with intact cognition. Those without objective evidence of cognitive impairment may, nevertheless, perceive themselves as having memory dysfunction, memory impairment ; it is these Patients, patients, Patient , therefore, who most require both subjective and objective assessments of cognition, including carers' assessments, in order to establish the nature of their symptoms. None of these assessment measures can be used as a reliable proxy for another, each contributes individually to a comprehensive assessment of cognition, and all must be used in conjunction with measures of mood and Patients, patients, Patient48 .
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1331_biomrc
|
Title: A staged approach to long gap esophageal atresia employing a XXXX and delayed reconstruction of the esophagus: an experimental study.
In beagle dogs , the cervical esophagus was divided 5 cm cranial to the thoracic inlet employing a stapler. The distal esophageal stump was attached to the external surface of the trachea . A spiral myotomy (2 1/2 revolutions) was made in a 3-cm long segment constituting the distal end of the proximal esophageal segment. This was twisted on a bias with the muscle edges approximated by interrupted stitches to cover the denuded submucosal layer. With moderate traction, this segment could be elongated to a length of 5 cm. A subcutaneous tunnel was created in the anterior chest to accommodate the reconstructed proximal esophageal segment (under slight traction), with its distal end forming a cutaneous esophagostomy. A gastrostomy was created using a Gauderer button (Bard Interventional Products, Billerica, MA) for feeding. After 3 weeks, the proximal esophageal segment was mobilized and removed from the subcutaneous tunnel. The distal esophageal segment was freed from the trachea and 5 to 8 cm of its proximal end was excised. The proximal (myotomized) esophagus was brought down to the stump of the remaining distal esophagus and an anastomosis formed in an end-to-end fashion. Oral feeding was reestablished within 1 week. Prolonged ingestion, observed soon after operation, gradually improved. During a period of 1 to 6 months after the operation, motility of the myotomized segment was tested by barium swallow and manometry. There was neither diverticulum formation nor stenosis . Transit of contrast material in the myotomized segment was smooth and rapid. Manometry demonstrated preservation of motility in the myotomized segment of the esophagus.(ABSTRACT TRUNCATED AT 250 WORDS)
| null |
1338_biomrc
|
Title: Hemodynamic effects of XXXX channel blockers at rest and during exercise in essential @entity101 .
The main hemodynamic disturbance occurring in patients, men with essential patients, men01 is an increase in the total peripheral resistance. In young patients, men with patients, men01 , this disturbance is clearly seen during muscular exercise, even though the calculated resistance might be normal during rest. This article reports results of studies on the long-term hemodynamic effects of two calcium channel blockers, patients, men855 and nifedipine , in patients, men with mild to moderate patients, men01 . Twenty-five patients, men , aged 20 to 64 years, with diastolic blood pressures between 100 and 120 mm Hg before treatment were studied at rest and during exercise on ergometer bicycles. Blood pressure was recorded intra-arterially, and cardiac output was measured. After this initial study, 10 patients, men were treated with patients, men855 (from 40 to 80 mg, three times daily) and 15 patients, men with nifedipine (long-acting form, from 40 to 80 mg daily). After one year, the hemodynamic study was repeated. Both drugs induced a reduction in blood pressure and in the total peripheral resistance without any reduction in the cardiac index. patients, men855 reduced heart rate, particularly during exercise, but this effect was compensated by an increase in the stroke volume. The hemodynamic profile of these two calcium channel blockers clearly differs from the hemodynamic effects of beta blockers.
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3391_biomrc
|
Title: Angina at @entity258 And Risk of imminent XXXX (the ALARM study): a prospective, observational proof-of-concept study.
BACKGROUND: MI, Myocardial infarction ( MI, Myocardial infarction ) is often preceded by patients, patient459 . Helping patients, patient identify the onset of patients, patient459 rather than MI, Myocardial infarction may result in earlier treatment and improve outcomes. patients, patient459 is beginning of angina, angina occurring at a lower-than-usual workload. Since heart rate (HR) is correlated with degree of exertion, we hypothesised that beginning of angina, angina occurring at low HR is a warning signal for patients, patient459 and MI, Myocardial infarction . METHODS: In this prospective study, 111 patients, patient with patients, patient187 ( patients, patient187 ) or prognostically significant coronary disease were recruited. Each patients, patient 's HR was measured using a portable electrocardiogram (ECG) recorder after regular class III exercise on the Canadian Cardiovascular Society Angina Grading Scale and the cumulative moving average and three-sigma (standard deviation) range were calculated for each new measurement. The HR was subsequently measured at the beginning of angina, angina ; a HR lower than the preceding three-sigma ranges for class III or anginal HR was regarded as a 'warning signal'. The proportion of warning signals associated with patients, patient187 occurring in the following 2 weeks was compared with that for non-warning signals. RESULTS: Nine cases of patients, patient187 occurred in eight patients, patient . Two cases were preceded by warning signals; a signal marked the onset of patients, patient187 in a third patients, patient , and four patients, patient failed to make anginal ECG recordings. There were 591 documented episodes of beginning of angina, angina during the study and ECGs were available for 383 (64.8 %) of these of which 55 were warning signals. Of these warning signals, 4 occurred in the 2 weeks preceding patients, patient187 , compared with 4 of 328 non-warning signals (odds ratio, 6.4; 95 % confidence interval, 1.5-26.2; p = 0.01; positive predictive value, 7.3 %; negative predictive value, 98.8 %). CONCLUSIONS: Low HR beginning of angina, angina may identify patients, patient459 and serve as an early warning for MI, Myocardial infarction . In addition, beginning of angina, angina that does not occur at a low heart rate indicates that patients, patient187 is very unlikely.
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1346_biomrc
|
Title: Reirradiation of locally recurrent XXXX .
PURPOSE: To study the efficacy of reirradiation as salvage treatment in patients, patient, PATIENTS with locally recurrent patients, patient, PATIENTS89 . patients, patient, PATIENTS AND METHODS: Between 1993 and 2000, 20 consecutive patients, patient, PATIENTS (twelve males and eight females) with tumor, nasopharyngeal cancer, tumors , previously irradiated in different Hungarian institutions, were reirradiated for biopsy-proven locally recurrent tumor, nasopharyngeal cancer, tumors . Histologically, 85% of the patients, patient, PATIENTS had WHO type III, 5% type II, and 10% type I disease. Stages I-IV (AJCC 1997 staging system) were assigned to five (25%), seven (35%), five (25%), and three (15%) patients, patient, PATIENTS , respectively; none of them had distant metastases, and only eight (40%) displayed regional dissemination. The median time period between termination of primary treatment and local recurrence was 30 (range, 10-204) months. Brachytherapy was the method most frequently used: in ten cases alone (especially for rT1 tumor, nasopharyngeal cancer, tumors ), and in eight cases in combination with external beam therapy. Two patients, patient, PATIENTS with locally advanced disease underwent external beam therapy only. The median dose in the event of brachytherapy alone was 20 Gy (4 x 5 Gy or 5 x 4 Gy, range, 16-36 Gy), and the dose range for exclusive external irradiation was 30-40 Gy. In cases of combined irradiation, a median 20-Gy brachytherapy (range, 16-40 Gy) was associated with 30-40 Gy of external irradiation. Radiotherapy was supplemented by neck dissection (six patients, patient, PATIENTS ), nasopharyngectomy (one patients, patient, PATIENTS ), or chemotherapy (eleven patients, patient, PATIENTS ). RESULTS: 16 patients, patient, PATIENTS were reirradiated once, three twice, and one patients, patient, PATIENTS three times, with a median equivalent dose for tumor, nasopharyngeal cancer, tumors effect of 36 Gy (mean, 44 Gy; range, 19-117 Gy; the estimated alpha/beta-ratio was 10 Gy). The median equivalent dose of reirradiation for late effect on normal tissue (with an estimated 70% delivery of the tumor, nasopharyngeal cancer, tumors dose) amounted to 30 Gy (mean, 37 Gy; range, 13-101 Gy, estimated alpha/beta-ratio 3 Gy). After a median follow-up of 37 (range, 12-72) months, the overall survival was 60% (12/20). Seven of the twelve surviving patients, patient, PATIENTS are currently tumor, nasopharyngeal cancer, tumors -free. After primary irradiation, xerostomy occurred in all patients, patient, PATIENTS as an unavoidable side effect of treatment. Following reirradiation, a severe (grade 3 or higher) late patients, patient, PATIENTS37 (CTC criteria, version 2) has been observed in two tumor, nasopharyngeal cancer, tumors -free patients, patient, PATIENTS (10%) so far ( necrosis of soft palate and paresis of glossopharyngeal nerve). CONCLUSION: Retreatment of patients, patient, PATIENTS89 with radiotherapy (preferably a combined modality), can result in longterm local control and survival in a substantial proportion of patients, patient, PATIENTS , at the price of an acceptable morbidity.
| null |
3398_biomrc
|
Title: Protective effects of a by-product of the pecan nut industry (Carya illinoensis) on the @entity137 induced by XXXX in @entity35 Carya illinoensis protects against @entity786 -induced @entity137 .
This study investigated the antioxidant effects of pecan nut (Carya illinoensis) shell aqueous extract (AE) on toxicity induced by cyclophosphamide (CP) in the heart, kidney, liver, bladder, plasma and erythrocytes of Rats, rats . Rats, rats were treated with water or pecan shell AE (5%) ad libitum, replacing drinking water for 37 days up to the end of the experiment. On day 30, half of each group received a single administration of vehicle or CP 200 mg/kg-ip. After 7 days, the organs were removed. Rats, rats treated with CP showed an increase in lipid peroxidation (LP) and decrease in reduced glutathione ( GSH ) levels in all structures. CAT, Catalase ( CAT, Catalase ) activity was increased in the heart and decreased in liver and kidney . Besides, CP treatment decreased plasmatic vitamin C (VIT C) levels and induced bladder macroscopical and microscopical damages. In contrast, co-treatment with pecan shell AE prevented the LP development and the GSH depletion in all structures, except in the heart and plasma, respectively. CAT, Catalase activity in the heart and liver as well as the plasmatic VIT C levels remained unchanged. Finally, AE prevented CP-induced bladder injury . These findings revealed the protective role of pecan shell AE in CP-induced multiple organ toxicity .
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3399_biomrc
|
Title: Incidence and Potential Mechanism(s) of Post-Procedural Rise of Cardiac Biomarker in @entity1 With Coronary Artery Narrowing After Implantation of an XXXX -Eluting Bioresorbable Vascular Scaffold or @entity2239 -Eluting Metallic Stent.
OBJECTIVES: This study sought to evaluate the mechanism of post-procedural cardiac biomarker (CB) rise following device implantation. BACKGROUND: A fully bioresorbable Absorb scaffold, compared with Everolimus, everolimus -eluting metallic stents (EES), might be associated with a higher incidence of periprocedural myocardial injury . METHODS: In 501 patients with stable or unstable angina randomized to either Absorb (335 patients ) or EES (n = 166) in the ABSORB II trial, 3 types of CB ( creatine kinase, creatine kinase-myocardial band, and troponin) were obtained before and after procedure. Per protocol, periprocedural myocardial infarction (PMI) was defined as creatine kinase rise >2* the upper limit of normal with creatine kinase-myocardial band rise. RESULTS: Incidence of side branch occlusion and any anatomic complications assessed by angiography was similar between the 2 treatment arms (side branch occlusion: Absorb: 5.3% vs. Xience: 7.6%, p = 0.07; any anatomic complication: Absorb: 16.4% vs. EES: 19.9%, p = 0.39). Fourteen patients who presented with recent myocardial infarction at entry with normalized creatine kinase-myocardial band according to the protocol were excluded for post-CB analysis. The overall compliance for CB was 97.8%. The CB rise subcategorized in 7 different ranges was comparable between the 2 treatment arms. PMI rate was numerically higher in the Absorb arm according to the per-protocol definitions, and treatment with overlapping devices was the only independent determinant of per-protocol PMI (odds ratio: 5.07, 95% confidence interval: 1.78 to 14.41, p = 0.002). CONCLUSIONS: There were no differences in the incidence of CB rise and PMI between Absorb and EES. Device overlap might be a precipitating factor of myocardial injury . (ABSORB II Randomized Clinical Trial: A Clinical Evaluation to Compare the Safety, Efficacy, and Performance of Absorb Everolimus, everolimus Eluting Bioresorbable Vascular Scaffold System Against Xience Everolimus, everolimus Eluting Coronary Stent System in the Treatment of Subjects With Ischemic Heart Disease Caused by De Novo Native Coronary Artery Lesions [ABSORB II]; NCT01425281).
| null |
3403_biomrc
|
Title: Probiotics for Prevention and Treatment of XXXX .
UNASSIGNED: Probiotics are increasingly used for prevention and treatment of diarrhea more in children, patient than in adults. Given the broad spectrum of diarrhea , this review focuses on the main etiologies: acute gastroenteritis , antibiotic-associated diarrhea (AAD), and NEC, NEC enterocolitis, necrotizing enterocolitis ( NEC, NEC enterocolitis, necrotizing enterocolitis ). For each, we reviewed randomized controlled trials, meta-analyses, and guidelines. For acute gastroenteritis we found 12 guidelines: 5 recommended probiotics and 7 did not. However, the guidelines containing positive recommendations provided proof of evidence from clinical trials and meta-analyses. children, patient905 (LGG) and Saccharomyces boulardii had the most compelling evidence of efficacy as they reduced the duration of the disease by 1 day. For AAD 4 meta-analyses were found, reporting variable efficacy of probiotics in preventing diarrhea , based on the setting, children, patient 's age, and antibiotics. The most effective strains were LGG and S. boulardii. For NEC, NEC enterocolitis, necrotizing enterocolitis , we found 3 randomized controlled trials, 5 meta-analyses, and 4 position papers. Probiotics reduced the risk of NEC, NEC enterocolitis, necrotizing enterocolitis and mortality in preterm babies. Guidelines did not support a routine use of probiotics and asked for further data for such sensitive implications. In conclusion, there is strong and solid proof of efficacy of probiotics as active treatment of children, patient902 in addition to rehydration. There is solid evidence that probiotics have some efficacy in prevention of AAD, but the number needed to treat is an issue. For both etiologies LGG and S. boulardii have the strongest evidence. In NEC, NEC enterocolitis, necrotizing enterocolitis the indications are more debated, yet on the basis of available data and their implications, probiotics should be carefully considered.
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3406_biomrc
|
Title: Behavior-dependent evoked potentials in the hippocampal @entity1249 region of the XXXX . I. Correlation with behavior and EEG.
As an alternative approach to the study of hippocampal function in relation to behavior, the averaged evoked potentials (AEPs) evoked by electrical stimulation of the Schaffer collaterals (SCH), the alveus and the contralateral hippocampus were recorded at various depths in the hippocampal CA1 region of freely moving rats with chronically implanted electrodes. Significant correlations between AEPs, behavior and EEG were found. At one end of the continuum of AEPs were those recorded during large irregular activity, LIA ( large irregular activity, LIA ), an EEG pattern associated with slow-wave sleep or awake-immobility. These AEPs had large early peak and low-amplitude late peaks. At the order end of the continuum, during high frequency theta EEG associated with behaviors such as walking, postural change or phasic paradoxical sleep. AEPs had a smaller early peak, increased later peak(s) and appeared oscillatory. The evoked population spike, a synchronous postsynaptic firing of CA1 neurons, was smaller during behaviors associated with theta than during those associated with large irregular activity, LIA . It is postulated that a recurrent inhibitory circuit within the hippocampus can account for the change of the AEPs with EEG and behavior and with stimulus intensity. During theta EEG, the negative feedback may increase such that the evoked population excitatory postsynaptic potential and the evoked population spike decrease and ascillatory response is more readily elecited. The excitability state of hippocampal CA1 may be described by the negative feedback gain in this model.
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3407_biomrc
|
Title: The attentional 'zoom-lens' in 8-month-old XXXX .
UNASSIGNED: The spatial attention mechanisms of orienting and zooming cooperate to properly select visual information from the environment and plan eye movements accordingly. Despite the fact that orienting ability has been extensively studied in infancy, the zooming mechanism - namely, the ability to distribute the attentional resources to a small or large portion of the visual field - has never been tested before. The aim of the present study was to evaluate the attentional zooming abilities of 8-month-old infants . An eye-tracker device was employed to measure the SLs, saccadic latencies ( SLs, saccadic latencies ) at the onset of a visual target displayed at two eccentricities. The size of the more eccentric target was adjusted in order to counteract the effect of cortical magnification. Before the target display, attentional resources were automatically focused (zoom-in) or spread out (zoom-out) by using a small or large cue, respectively. Two different cue-target intervals were also employed to measure the time course of this attentional mechanism. The results showed that infants ' SLs, saccadic latencies varied as a function of the cue size. Moreover, a clear time course emerged, demonstrating that infants can rapidly adjust the attentional focus size during a pre-saccadic temporal window. These findings could serve as an early marker for neurodevelopmental disorders associated with attentional zooming dysfunction such as autism and dyslexia .
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3409_biomrc
|
Title: Safety and Feasibility of Same-Day Discharge in Obese XXXX Undergoing Laparoscopic Hysterectomy for @entity1478 and Malignancy [335].
INTRODUCTION: Hysterectomy for endometrial cancer is increasingly performed by laparoscopic surgical technique. Same-day discharge is safe, cost-saving, and acceptable to patients undergoing laparoscopic hysterectomy, but concerns about increased perioperative risks in obese, obesity patients limit the utilization of same-day discharge in this population. We evaluated the risk of perioperative complications in obese, obesity patients undergoing laparoscopic hysterectomy for endometrial cancer or patients478 in our institution during a period of rapid increase in the utilization of same-day discharge. METHODS: Single-institution retrospective cohort including all obese, obesity patients (body mass index [calculated as weight (kg)/[height (m)]] 30) undergoing laparoscopic hysterectomy for endometrial cancer or patients478 between 2011 and 2013. RESULTS: A total of 388 patients were included. Those with class III obese, obesity were younger than those with class I or II (58.4 compared with 63.0 years, P<.001) and less likely to have invasive endometrial cancer (80.9% compared with 88.7%, P=.04). Those with class III obese, obesity were significantly more likely to undergo robot-assisted laparoscopy than those with class I or II obese, obesity (57.5% compared with 18.6%, P<.001). The rate of same-day discharge increased significantly over the 3-year study period: for those with class I and II obese, obesity from 18.6% to 65.5% and for those with class III obese, obesity from 12.7% to 61.4% (P<.001). During this period there were no significant differences in frequency of postoperative complications within 30 days of surgery, including readmission, unscheduled surgery, or obese, obesity1 (P=.1 for all). CONCLUSION: Same-day discharge is safe and feasible for the majority of obese, obesity patients undergoing laparoscopic surgery for endometrial cancer and patients478 .
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1362_biomrc
|
Title: Autophagy through @entity5291 and XXXX regulates oxidative stress-induced premature senescence in auditory cells.
UNASSIGNED: The aim of this study was to determine whether autophagy and AMPK contribute to premature senescence in auditory cells. Incubating HEI-OC1 auditory cells with 5 mM H2O2 for 1 h induced senescence, as demonstrated by senescence-associated b-galactosidase (SA-b-gal) staining. H2O2 treatment significantly delayed population-doubling time, leaving cell viability unchanged. Furthermore, the proportion of SA-b-gal-positive cells significantly increased. Autophagy-related protein expression increased, with Atg7 and LC3-II peaking 6 h and Lamp2 peaking 24 h after H2O2 treatment. The expression of these proteins decreased 48 h after treatment. Transmission electron microscopy revealed lipofuscin and aggregates within autolysosomes, which accumulated markedly in the cytoplasm of HEI-OC1 cells 48 h after treatment. Akt and P70S6 phosphorylation markedly decreased after H2O2 treatment, but 4EBP1 phosphorylation significantly increased 48 h after treatment. After RNAi-mediated knockdown (KD) of Atg7 and AMPK , H2O2 -treated cells displayed dense SA-b-gal staining. Also, premature senescence was significantly induced. These suggest that a negative feedback loop may exist between autophagy and AMPK signaling pathways in HEI-OC1 cells. In our model, oxidative stress-induced premature senescence occurred due to impaired autophagy function through 4EBP1 phosphorylation. Our results also indicate that AMPK may regulate premature senescence in auditory cells in an autophagy-dependent and independent manner.
| null |
3412_biomrc
|
Title: Age-related expression of @entity876 , @entity7465 , @entity470 and XXXX in @entity463 multiforme.
OBJECTIVE: GBM, Glioblastoma multiforme ( GBM, Glioblastoma multiforme ) is a major cause of morbidity and mortality in neurosurgical patients, patient . Despite the overall poor prognosis a range in survival times exists. Many approaches have been undertaken to define patients, patient subgroups based on molecular changes. The aim of this study was to assess a possible correlation between the immunohistochemical P53, p53 , Mdm2 , EGFR and Msh2 expression and age. METHOD: 143 patients, patient (77 male, 66 female) were included in this retrospective study who underwent craniotomy for newly-diagnosed GBM, Glioblastoma multiforme between May 1994 and February 2000. For statistical analysis, patients, patient were separated into three age groups: 1. < 40 years, 2. 40-60 years, 3. > 60 years. Immunohistochemical staining (IHC) was performed using anti- P53, p53 (clone DO-1), anti- Mdm2 (clone IF-2 ), anti- EGFR (clone H11) and anti- Msh2 antibodies (clone AB-1). The results were compared with the Ki67/ patients, patient758 proliferation index (Ki67 PI) and patients, patient survival. FINDINGS: P53, p53 protein expression was significantly decreasing with advanced age (p < 0.05) whereas EGFR and Mdm2 expression was increasing (p < 0.05; p=0.01). Msh2 expression was unrelated to age. Multivariate analysis revealed Msh2 protein expression as a significant predictor of prolonged survival (p=0.004) whereas P53, p53 , Mdm2 and EGFR were not associated with patients, patient survival. P53, p53 , Mdm2 , EGFR and Msh2 expression was not associated with the Ki67 PI. INTERPRETATION: Our results support the hypothesis that in GBM, Glioblastoma multiforme patients, patient a complex relationship exists between the P53, p53 , Mdm2 and EGFR expression and age. Msh2 expression is not related to age. Notably, nuclear Msh2 expression turned out to be an independent prognostic indicator.
| null |
1365_biomrc
|
Title: A microarray based expression profiling of @entity1311 and XXXX resistant MCF-7 cells.
Resistance to the broad spectrum of chemotherapeutic agents in cancer, tumors cell lines and cancer, tumors has been called multiple drug resistance (MDR). In this study, the molecular mechanisms of resistance to two anticancer agents ( paclitaxel and mammary carcinoma1 ) in mammary carcinoma cell line MCF-7 were investigated. Drug resistant sublines to paclitaxel (MCF-7/Pac) and mammary carcinoma1 (MCF-7/ Vinc ) that were developed from sensitive MCF-7 cells (MCF-7/S) were used. cDNA microarray analysis was performed for the RNA samples of sensitive and resistant cells in duplicate experiments. GeneSpring GX 7.3.1 Software was used in data analysis. The results indicated that the upregulation of MDR1 gene is the dominating mechanism of the paclitaxel and mammary carcinoma1 drug resistance. Additionally the upregulation of the genes encoding the detoxifying enzymes (i.e. GSTP1 ) was observed. Significant downregulation of apoptotic genes (i.e. PDCD2/4/6/8 ) and upregulation of some cell cycle regulatory genes ( CDKN2A , CCNA2 etc.) was seen which may be in close relation to MDR in breast cancer . Drug resistant cancer, tumors cells exhibit different gene expression patterns depending on drug treatment, and each drug resistance phenotype is probably genetically different. Further functional studies are needed to demonstrate the complete set of genes contributing to the drug resistance phenotype in breast cancer cells.
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1371_biomrc
|
Title: Biochemical genetics of the cryptic gene system for @entity8692 utilization in XXXX .
The cellobiose catabolic system of E. coli K12, Escherichia coli K12 is being used to study the role of cryptic genes in microbial evolution. Wild-type E. coli K12, Escherichia coli K12 do not utilize the beta-glucoside sugars , arbutin , salicin and cellobiose . A Cel+ ( cellobiose utilizing) mutant which grows on cellobiose , arbutin , and salicin was isolated previously from wild-type E. coli K12, Escherichia coli K12 . Biochemical assays indicate that a cel structural gene (celT) specifies a single transport protein that is a beta-glucoside specific enzyme of the phosphoenolpyruvate -dependent phosphotransferase system. The transport protein phosphorylates beta-glucosides at the expense of phosphoenolpyruvate. A single phosphoglucosidase, specified by celH, hydrolyzes phosphorylated cellobiose , arbutin , and salicin . The genes of the cel system are expressed constitutively in the Cel+ mutant, whereas they are not expressed at a detectable level in the wild-type strain. The transport and hydrolase genes are simultaneously silenced or simultaneously expressed and thus constitute an operon. Cel+ strains which fail to utilize one or more beta-glucosides express the transport system at a lower level than do Cel+ strains which grow on all three beta-glucosides. Other strains inducibly express a gene which specifies transport of arbutin but not the other beta-glucosides . The arbutin transport gene, arbT, maps outside of the cel locus.
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3423_biomrc
|
Title: Variation in Inflammatory Response during XXXX Is Influenced by Host-Pathogen Interactions but Associated with Animal Survival.
Inflammation, inflammation is a crucial part of innate immune responses but, if imbalanced, can lead to serious clinical conditions or even death. Cytokines regulate Inflammation, inflammation , and studies report their impact on clinical outcome. However, host and pathogen genetic backgrounds influence cytokine production, making it difficult to evaluate which inflammatory profiles (if any) relate to improved prognosis.Streptococcus pneumoniaeis a common human pathogen associated with asymptomatic nasopharyngeal carriage. Infrequently, it can lead to a wide range of diseases with high morbidity and mortality rates. Studies show that both pneumococcal serotype and host genetic background affect the development of disease and contribute to variation in inflammatory responses. In this study, we investigated the impact of the host and pneumococcal genetic backgrounds on pulmonary cytokine responses and their relationship to animal survival. Two inbred human9 strains, BALB/c and CBA/ Ca , were infected with 10 pneumococcal strains, and the concentrations of six pulmonary cytokines were measured at 6 h and 24 h postinfection. Collected data were analyzed by principal-component analysis to identify whether there is any pattern in the observed cytokine variation. Our results show that host-pneumococcus combination was at the core of observed variation in cytokine responses, yet the resulting cytokine profile discriminated only between survivors and fatalities but not human9 or pneumococcal strains used during infection. Therefore, our results indicate that although alternative inflammatory profiles are generated during pneumococcal infection, pneumococcal infections , a common pattern emerged, which determined the clinical outcome of pneumococcal infection, pneumococcal infections .
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1376_biomrc
|
Title: [Efficacy and safety of inhalation premixed XXXX and @entity26 for the management of procedural diagnostic @entity158 in neuropediatrics].
AIM: We studied the use of premixed nitrous oxide and oxygen in 80 children, patients, PATIENTS with children, patients, PATIENTS6 . children, patients, PATIENTS AND METHODS: Mean ages ranged 10 +/- 5 yrs. Twenty-three children, patients, PATIENTS (29%) were children, patients, PATIENTS032 among which 17 of them presented with severe epilepsy . children, patients, PATIENTS58 procedures consisted of: lumbar punctures (80%), intravenous access (7), gastric endoscopy (6), skin biopsy (4), gastrostomy tube management (3). High-risk children, patients, PATIENTS were continuously monitored using ECG, non invasive blood pressure and transcutaneous oxygen saturation. We studied acceptation of the inhalation, vital signs, satisfaction of children, patients, PATIENTS , parents, medical and nursing staffs; side effects were compared with a group of healthy children, patients, PATIENTS undergoing venous access before induction of anesthesia. RESULTS AND DISCUSSION: Acceptation increased with age. No significant changes in vital signs variables were observed. Satisfaction rate regarding the method was 88% for all children, patients, PATIENTS , parents, physicians and nurses. No serious undesirable event (as children, patients, PATIENTS576 , seizure , inhalation of gastric content) occurred in these children, patients, PATIENTS . The more frequent side-effects were: drowsiness during and after inhalation (35 and 9% respectively in the handicapped children, patients, PATIENTS ); nausea and vomiting (8%), children, patients, PATIENTS0 (3%), were more frequent than reported in literature but there were 25% of meningitis among our children, patients, PATIENTS . CONCLUSION: Premixed nitrous oxide and oxygen was effective for reducing procedural children, patients, PATIENTS58 and children, patients, PATIENTS48 in children, patients, PATIENTS with children, patients, PATIENTS6 , even in severely handicapped children, patients, PATIENTS , with minor side-effects.
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1377_biomrc
|
Title: Valved or valveless ventriculoperitoneal shunting in the treatment of post- XXXX @entity1276 : a population-based consecutive cohort study.
BACKGROUND: Implant infection, shunt infection and obstruction are major complications for ventriculoperitoneal shunts in Patients, patients, patient with post- haemorrhagic Patients, patients, patient276 . In an effort to (1) reduce the incidence of these complications, (2) reduce the rate of shunt failure and (3) shorten the duration of neurosurgical hospitalisation, we have implemented valveless ventriculoperitoneal shunts at our department for adult Patients, patients, patient with post- haemorrhagic Patients, patients, patient276 and haemorrhagic cerebrospinal fluid at the time of shunt insertion. METHODS: All adult Patients, patients, patient (>18 years old) treated for post- haemorrhagic Patients, patients, patient276 with ventriculoperitoneal shunting at our institution from 1 January 2008 to 31 December 2014 were included in this retrospective population-based consecutive cohort study. Data were collected by retrospectively reviewing medical records. We compared two different shunt modalities (valveless vs valve-regulated), analysing frequencies of complications, shunt survival and duration of neurosurgical hospitalisation. RESULTS: A total of 214 Patients, patients, patient aged 22-86 (mean age, 60.5 11.5 years) were included, comprising 137 valveless and 77 valve-regulated shunts. We found no difference in the rate of surgical shunt revision (p = 0.65) or differences in time interval from insertion to first surgical revision (p = 0.31) between the two shunt modalities. The duration of neurosurgical hospitalisation was shorter for Patients, patients, patient receiving a valveless shunt (p = 0.004). Patients, patients, patient with valveless shunts had a lower rate of Implant infection, shunt infection (5.1 % vs 14.3 %, p = 0.02), but a higher rate of overdrainage (10.3 % vs 2.6 %, p = 0.04). CONCLUSION: The use of a valveless shunting for Patients, patients, patient with post- haemorrhagic Patients, patients, patient276 results in shorter duration of neurosurgical hospitalisation and lower rate of Implant infection, shunt infection , although these advantages should be held up against the risk of overdrainage. We propose valveless shunting to be used as first-line shunting strategy in this Patients, patients, patient category, with careful follow-up ensuring that these are substituted by a valve-bearing system if necessary.
| null |
3439_biomrc
|
Title: Fundus autofluorescence of elongated photoreceptor outer segments in XXXX .
PURPOSE: To determine the origin of fundus autofluorescence (AF) patterns in central serous chorioretinopathy, CSC ( central serous chorioretinopathy, CSC ). DESIGN: Retrospective, observational case series. METHODS: We retrospectively studied 30 consecutive eyes of 30 patients with primary central serous chorioretinopathy, CSC using AF and spectral-domain optical coherence tomography (SD-OCT). We measured the AF using the Heidelberg Retina Angiograph with a 488-nm excitation light and a 500-nm cutoff barrier filter and compared the AF patterns with ophthalmoscopy and SD-OCT. RESULTS: We observed a patchy increased AF in the macular area in 22 eyes (73%), in which the length of the photoreceptor outer segment at the central fovea tended to be longer than the other eyes (P=.06). The punctate increased AF corresponded to the ophthalmoscopic precipitates in 17 eyes with precipitates. AF significantly (P=.017) decreased in eyes with a patients713 ( patients713 ). Eight eyes (27%) had increased AF in the inferior patients713 . CONCLUSIONS: The patchy increased AF appears to originate from elongated photoreceptor outer segments in the patients1471 . The autofluorescent fluorophores from the photoreceptor outer segments may be concentrated in precipitates or have settled into the inferior patients713 .
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1392_biomrc
|
Title: Predictors of @entity64 and XXXX in thoracic aortic endovascular intervention.
BACKGROUND: Endoluminal repair of thoracic aortic pathology has become established in clinical practice, but is associated with significant women, patients, Patient, men6 . The aim of this study was to identify factors that were predictive of stroke, Stroke and Paraplegia, paraplegia . METHODS: Prospective data was collected for a cohort of 293 consecutive women, patients, Patient, men having thoracic aortic endovascular repair between August 1997 and September 2009. women, patients, Patient, men and procedural characteristics were related to the incidence of stroke, Stroke and Paraplegia, paraplegia using multivariate logistic regression analysis. RESULTS: The median age was 68 years (18-87), there were 191 women, patients, Patient, men and 102 women, patients, Patient, men . Mortality was 5.1% for 195 elective and 13.4% for 98 urgent women, patients, Patient, men . stroke, Stroke affected 16 (5.5%) women, patients, Patient, men : 11 affected the anterior and 5 the posterior circulation. Coverage of the left subclavian artery with no revascularisation was the only significant factor predictive of stroke, Stroke (OR 5.34 (1.42-20.40) P = 0.01). Paraplegia, paraplegia affected 16 women, patients, Patient, men (5.5%) but no independent risk factor was identified: 12 were identified perioperatively and 4 were delayed by up to 6 months. CONCLUSION: Covering the left subclavian artery without revascularisation increases the risk of stroke, Stroke following endoluminal repair of thoracic pathology. Paraplegia, paraplegia appears to be more complex and no independent precipitating factor was identified.
| null |
3441_biomrc
|
Title: Increased blood-brain barrier permeability in XXXX demonstrated by @entity2287 magnetic resonance imaging.
OBJECTIVES: Patients, patients with type II diabetes are at increased risk of cognitive impairment . The retinal and renal complications of diabetics, diabetic, diabetes follow microvascular damage permitting small arterioles to leak, hence the cerebral damage might also follow loss of blood-brain barrier (BBB) integrity. Magnetic resonance (MR) brain imaging with intravenous gadolinium (Gd) diethylenetriamine pentaacetic acid ( Gd-DTPA ) was used to identify increased BBB permeability. METHODS: Ten well controlled type II diabetics, diabetic, diabetes Patients, patients aged 65-70 years and 10 controls underwent MR brain imaging with fluid attenuated inversion recovery (FLAIR); T1 weighted (T1W) volumetric imaging before; and T1W volumetric imaging at 5, 15, 30, 45, 60, and 90 minutes after intravenous Gd-DTPA . The T1W image before Gd-DTPA was subtracted from the images at each time point after Gd-DTPA . Net signal intensity was plotted against time for different brain regions. diabetics, diabetic, diabetes2 were scored from the FLAIR image. RESULTS: The signal intensity/time curves showed that brain signal intensity increased more in the diabetics, diabetic, diabetes group than controls during the first 15 minutes after Gd-DTPA , particularly in the basal ganglia (p=0.018). Signal intensity in controls peaked at five minutes and diabetics, diabetic, diabetes at 15 minutes. Subjects with more diabetics, diabetic, diabetes2 had greater signal increase after Gd-DTPA , whether diabetics, diabetic, diabetes or not (p=0.001). CONCLUSIONS: Increased BBB permeability with MR imaging was detected in Patients, patients with diabetics, diabetic, diabetes2 . Increased permeability of the BBB might account for some of the cerebral effects of type II diabetes , and so possibly also for the effect of other conditions that affect the microvasculature (like Patients, patients01 ), on the brain.
| null |
3448_biomrc
|
Title: Prevalence of precipitating antibodies to different extracts of XXXX in a North American @entity748 population.
This study was carried out to find the prevalence of precipitin reactions in the sera of 200 North American asthmatic subjects. Precipitins were detected by the double diffusion technique using different extracts of Aspergillus fumigatus , including a reference 'home produced' extract and five commercial extracts from three different suppliers. In addition, antigenicity of these extracts was assessed by crossed immunoelectrophoresis (XIE). Of the sera, 13.5% reacted to the reference extract and from 2.5 to 12% to the different commercial extracts; 22.5% of the sera reacted to at least one extract. No one serum reacted to all the extracts. Two of fifty-one (4%) nonatopic patients with a negative immediate prick test to A. fumigatus, six of eighty-seven (6.9%) patients097 patients with a negative immediate reaction to A. fumigatus, and thirty-seven of sixty two (59%) patients097 patients with a positive immediate reaction to A. fumigatus had precipitins to at least one of the extracts used, the skin tests being performed using the A. fumigatus reference extract. The prevalence of precipitin reactions bore a strong correlation with the antigenicity of the extracts by XIE. The same reference extract was also used for specific IgE measurements (Brompton extract, Malo _ Paquin, 1979). It was found that patients with precipitins had significantly (P less than 0.001) higher specific and total IgE values than patients without precipitins. In the group of patients with positive skin test, those with precipitins had significantly (P less than 0.05) higher specific IgE values than those without. The authors conclude that different extracts of A. fumigatus should be used to assess the presence of precipitins. The antigenicity of these extracts should also be assayed.
| null |
1401_biomrc
|
Title: Pulsed dye laser treatment of XXXX : a randomized clinical pilot study comparison of 607- and 595-nm wavelength lasers.
BACKGROUND: The 595-nm pulsed dye laser has been used for the treatment of benign epidermal pigmented lesions (EPLs), but there is a risk of inducing undesirable purpura with treatment. OBJECTIVE: To compare a 607-nm laser with a commercially-available 595-nm laser for the treatment of EPLs. MATERIALS AND METHODS: Monte-Carlo simulations were performed to characterize laser interaction with skin. Ten Patients, patients, patient with EPLs were treated with a 607-nm study prototype laser and the 595-nm pulsed dye laser twice at 2- to 4-week intervals on the left or right side on a randomized basis. Study endpoints included clearance rate of lesions, side effects immediately after treatment and at final follow-up, and Patients, patients, patient discomfort/ Patients, patients, patient58 . RESULTS: Monte-Carlo simulations show that the 607-nm is absorbed more specifically by melanin than the 595-nm wavelength. Both lasers were effective in treatment of EPLs. The average degree of improvement overall was 41.2% with the 607-nm laser and 40% with the 595-nm laser. Patients, patients, patient reported less discomfort/ Patients, patients, patient58 during treatment with the 607-nm laser. CONCLUSIONS: Our findings suggest that the 607-nm laser is safe and at least as effective as the 595-nm laser in treatment of EPLs. There was less Patients, patients, patient discomfort/ Patients, patients, patient58 during treatment using the 607-nm laser.
| null |
3456_biomrc
|
Title: Targeting XXXX telomerase by antisense oligonucleotides and ribozymes.
human, Human telomerase is a ribonucleoprotein enzyme complex that enables cells to maintain telomere length, allowing indefinite replicative capacity. The notion that telomerase is reactivated in 80-90% of human, Human tumor, vivo tumor, cancers, cancer has led to the proposal of telomerase as a promising therapeutic target for novel anticancer interventions. Due to its inherent accessibility to nucleic acids, telomerase appears an ideal target for strategies based on the use of antisense oligonucleotides and ribozymes that target its RNA template. In this review a summary of the different antisense- and ribozyme-based approaches used thus far to inhibit telomerase activity in human, Human tumor, vivo tumor, cancers, cancer cells is provided. All these strategies significantly inhibited the enzyme's catalytic activity in in vitro and in tumor, vivo tumor, cancers, cancer models. However, while in some studies tumor, vivo tumor, cancers, cancer cell growth arrest was observed as a consequence of telomere shortening after prolonged telomerase inhibition, other studies have shown that antisense- and ribozyme-based treatments targeting telomerase induced rapid loss (i.e. within a few days) of tumor, vivo tumor, cancers, cancer cell viability with concomitant apoptosis. In the latter case it is unlikely that cell death was related to telomere erosion since the cells would not have undergone enough divisions to significantly shorten their telomeres. A possible explanation is that telomerase inhibitors may induce apoptosis in tumor, vivo tumor, cancers, cancer cells directly by interfering with the capping function of the enzyme. Overall, the available results indicate antisense oligonucleotides and ribozymes as good tools to inhibit telomerase and suggest that abrogation of telomerase activity may affect tumor, vivo tumor, cancers, cancer cell proliferation also through pathways that are not dependent on telomere erosion .
| null |
3464_biomrc
|
Title: Relationship between carotid @entity729 detected in dental panoramic images and @entity101 and XXXX .
BACKGROUND: Carotid artery calcification may be related to cerebrovascular accident, CVA , which may result in death or physical and mental disabilities in survivors. OBJECTIVES: Our purpose is to study the association of carotid artery calcification (CAC) on dental panoramic radiographs and two risk factors of cerebrovascular accident, CVA ( cerebrovascular accident, CVA ) including women, patients, PATIENTS, people01 and myocardial infarction, MI ( myocardial infarction, MI ). women, patients, PATIENTS, people AND METHODS: Panoramic images of 200 women, patients, PATIENTS, people that were all women, patients, PATIENTS, people above 50 years of age (a population suffering from vascular diseases ) were investigated. All panoramic images were provided under similar conditions in terms of the type of panoramic radiograph equipment, type of applied films and the automatic film processor. Then, the women, patients, PATIENTS, people answered questions about myocardial infarction, MI history and taking antihypertensive drugs. We also measured the blood pressure of women, patients, PATIENTS, people in two separate surveys. Data analysis was performed by SPSS statistical program. We used Exact Fisher test and Chi-Square test at a significant level of less than 0.05 to study the effect of these variables on the occurrence of carotid artery calcification . RESULTS: Among 200 studied samples, 22 of the women, patients, PATIENTS, people (11%) had carotid artery calcification on the dental panoramic radiograph. In total, 52 women, patients, PATIENTS, people (26%) had women, patients, PATIENTS, people01 and four women, patients, PATIENTS, people (2%) had a history of myocardial infarction, MI . Eleven individuals among women, patients, PATIENTS, people suffering from women, patients, PATIENTS, people01 (21.2%) and three individuals among women, patients, PATIENTS, people with a history of myocardial infarction, MI (75%) demonstrated CAC on dental panoramic images . CONCLUSIONS: The relationship between CAC found on dental panoramic radiographs and two cerebrovascular accident, CVA risk factors-- women, patients, PATIENTS, people01 and myocardial infarction, MI -- was significant. Therefore, it seems that detection of CAC on panoramic images of dental women, patients, PATIENTS, people must be considered by dentists.
| null |
3469_biomrc
|
Title: Metabolism of orally and intravenously administered purines in XXXX .
One experiment was conducted in which radioactively labeled purine bases ( adenine , guanine , hypoxanthine and xanthine ) were individually given intravenously to young adult rat, rats and the recovery of radioactivity in urine and gut, gut content and liver was measured at the end of the next 24 hours. The total recovery of radioactivity from orally and intravenously administered adenine was measured in experiment 2. A third experiment measured the recoveries of radioactivity from oral and intravenous adenine in a wider variety of tissues and organs than in experiment 1. The chemical identities of the urinary end products of the metabolism or orally and intravenously administered adenine were compared in a fourth experiment. When purines were given intravenously, significantly more of the administered radioactivity was recovered in urine from rat, rats given guanine , hypoxanthine or xanthine compared with those given adenine . The greater recoveries of radioactivity in urine were associated with smaller recoveries in tissues. A larger proportion of intravenously compared to orally administered radioactivity from adenine was incorporated into all body tissues, and this was most pronounced in glandular and lymphoid tissues. The primary urinary end product of both orally and intravenously administered adenine was allantoin. The absorption of individual purines from isolated rat, rats gut sacs was evaluated in a fifth experiment. A significant proportion of unaltered adenine crossed the mucosal to serosal barrier of intestinal sacs whereas unaltered guanine , hypoxanthine or xanthine did not cross into the serosal fluid. These results show that the intestinal metabolism of dietary adenine is uniquely different from that of guanine , hypoxanthine or xanthine .
| null |
1423_biomrc
|
Title: Higher weight at birth is related to decreased maternal XXXX oxidation during pregnancy.
BACKGROUND: Small size at birth is associated with cardiovascular disease in adult life. Decreased fetal growth may result from a limitation in the nutrient supply to the fetus. Net tissue deposition in the mother and fetus increases the demand for women, woman, infants, infant568 , but because maternal consumption of protein does not increase, there must be a change in the partitioning of women, woman, infants, infant522 , away from oxidation and toward deposition. OBJECTIVE: Our objective was to characterize amino acid, Amino acid oxidation in pregnancy and to investigate whether the relative partitioning of women, woman, infants, infant522 was related to fetal growth. DESIGN: We determined amino acid, Amino acid oxidation as women, woman, infants, infant133 production in 25 women, woman, infants, infant during mid (17-19 wk) and late (26-29 wk) gestation. women, woman, infants, infant133 production was measured from urinary [ women, woman, infants, infant3140 -(15)N]urea excretion over 48 h after a single oral dose of [ women, woman, infants, infant3140 -(15)N]urea. We measured the women, woman, infants, infant 's size at birth. RESULTS: For the group as a whole, women, woman, infants, infant133 excretion decreased and amino acid, Amino acid oxidation remained similar between mid and late pregnancy, but there was wide variation between the women, woman, infants, infant . Heavier women, woman, infants, infant were born to the mothers in whom amino acid, Amino acid oxidation decreased the most during pregnancy (slope of regression line: -80 g x g N(-1) x d(-1); 95% CI: -129, -31; P = 0.003). After adjustment for length of gestation and the women, woman, infants, infant 's sex, the change in maternal amino acid, Amino acid oxidation explained 34% of the variation in birth weight. CONCLUSIONS: amino acid, Amino acid oxidation varied widely between the women, woman, infants, infant during pregnancy. Understanding the ability of a pregnant women, woman, infants, infant to adapt metabolically may have implications for establishing dietary recommendations in pregnancy.
| null |
3474_biomrc
|
Title: Low-Dose Parenteral @entity1460 Oil for the Prevention of Parenteral Nutrition-Associated @entity409 in Neonates With XXXX : A Multicenter Randomized Controlled Pilot Study.
BACKGROUND: Neonates with gastrointestinal disorders, GDs ( gastrointestinal disorders, GDs ) are at high risk for PNALD, parenteral nutrition-associated liver disease ( PNALD, parenteral nutrition-associated liver disease ). patients460 -based intravenous lipid emulsions (S-ILE) have been associated with PNALD, parenteral nutrition-associated liver disease . This study's objective was to determine if a lower dose compared with a higher dose of S-ILE prevents patients448 without compromising growth. MATERIALS AND METHODS: This multicenter randomized controlled pilot study enrolled patients with gastrointestinal disorders, GDs who were <=5 days of age to a low dose (~1 g/kg/d) (LOW) or control dose of S-ILE (~3 g/kg/d) (CON). The primary outcome was patients448 (direct bilirubin [DB] >2 mg/dL) after the first 7 days of age. Secondary outcomes included growth, PN duration, and late-onset sepsis . RESULTS: Baseline characteristics were similar between the LOW (n = 20) and CON groups (n = 16). When the LOW group was compared with the CON group, there was no difference in patients448 (30% vs 38%, P = .7) or secondary outcomes. However, mean SE DB rate of change over the first 8 weeks (0.07 0.04 vs 0.3 0.09 mg/dL/wk, P = .01) and entire study (0.008 0.03 vs 0.2 0.07 mg/dL/wk, P = .02) was lower in the LOW group compared with the CON group. CONCLUSION: In neonates with gastrointestinal disorders, GDs who received a lower dose of S-ILE, DB increased at a slower rate in comparison to neonates who received a higher dose of S-ILE. Growth was comparable between the groups. This study demonstrates a need for a larger, randomized controlled trial comparing 2 different S-ILE doses for patients448 prevention in neonates at risk for PNALD, parenteral nutrition-associated liver disease .
| null |
3475_biomrc
|
Title: Resident and infiltrating cells in the @entity35 iris during the early stages of experimental melanin protein-induced XXXX (EMIU).
Experimental melanin protein-induced uveitis (EMIU) is reported to be a model of anterior uveitis and choroiditis in which the retina is spared. In this study, we chose to compare EMIU with experimental autoimmune uveoretinitis (EAU), a well-recognised model of endogenous posterior uveitis , with regard to the nature and dynamics of the cellular infiltrate in the iris. Female Lewis albino rats were immunised with mixtures of crude retinal extract/complete Freund's adjuvant ( CFA ) ( EAU ), phosphate -buffered saline/ CFA (controls), or iris melanin/ CFA (EMIU) using established protocols. Animals were sacrificed on days 10 and 13 (around disease onset). Following whole body perfusion fixation, irides were dissected from the remainder of the globe. Iris wholemount preparations were then subjected to immunohistochemical analysis in order to investigate both the dynamics of infiltrating leukocytes and the effects of the inflammatory changes on resident immune cells in the iris. The nature of the cellular infiltrate in both EMIU and EAU models was essentially similar, namely there was a rich infiltrate of EDI+ mononuclear cells, Ox42+ neutrophils and T cells. Resident tissue macrophages (ED2+) were slightly below normal densities in the iris of EAU animals and marginally elevated in EMIU animals, MHC class II (Ia) staining, associated in the normal eye with dendritic cells (DC), was considerably elevated in EMIU. It is likely that this was due to both increased DC numbers and an influx of Ia+ exudate macrophages. No striking difference was found in the nature and phenotype of the cellular infiltrate in the iris at the onset of the disease in these two models of uveitis (EAU and EMIU). This suggests that the anterior segment inflammation in both models represents non-specific changes secondary to cytokine release associated with interaction of activated antigen-specific T cells and target antigens, namely retinal photoreceptors in EAU and uveal tract melanin-containing cells in EMIU. Alternatively, it may suggest that antigen-presenting cells resident in the iris and ciliary body in normal eyes have access to ocular antigens on both sides of the blood-ocular barrier and are capable of activating circulating antigen-specific T cells in these models.
| null |
1427_biomrc
|
Title: The mare as a model for luteinized XXXX : intrafollicular endocrine milieu.
UNASSIGNED: Luteinized unruptured follicle (LUF) syndrome is a recurrent anovulatory dysfunction that affects up to 23% of women, human with normal menstrual cycles and up to 73% with endometriosis . Mechanisms underlying the development of LUF syndrome in mares were studied to provide a potential model for women, human anovulation . The effect of extended increase in circulating LH achieved by administration of recombinant women, human641 LH (reLH) or a short surge of LH and decrease in women, human420 induced by prostaglandin F2a, PGF2a ( prostaglandin F2a, PGF2a ) on LUF formation (Experiment 1), identification of an optimal dose of women, human2567 inhibitor ( flunixin-meglumine , FM; to block the effect of prostaglandins ) for inducing LUFs (Experiment 2), and evaluation of intrafollicular endocrine milieu in LUFs (Experiment 3) were investigated. In Experiment 1, mares were treated with reLH from Days 7-15 (Day 0 = ovulation), prostaglandin F2a, PGF2a on Day 7, or in combination. In Experiment 2, FM at doses of 2.0 or 3.0 mg/kg every 12 h and hCG (1500 IU) were administered after a follicle >= 32 mm was detected. In Experiment 3, FM at a dose of 2.0 mg/kg every 12 h plus hCG was used to induce LUFs and investigate the intrafollicular endocrine milieu. No LUFs were induced by reLH or prostaglandin F2a, PGF2a treatment; however, LUFs were induced in 100% of mares using FM. Intrafollicular prostaglandin F2a, PGF2a metabolite (PGFM), prostaglandin F2a, PGF2a , and women, human421 were lower and the ratio of women, human421 : prostaglandin F2a, PGF2a was higher in the Induced LUF group. Higher levels of intrafollicular E2 and total primary sex steroids were observed in the Induced LUF group along with a tendency for higher levels of GH, VEGF-A01 , and T; however, LH , PRL, VEGF-A , and NO did not differ between groups. In conclusion, this study reveals part of the intrafollicular endocrine milieu and the association of prostaglandins in LUF formation, and indicates that the mare might be an appropriate model for studying the poorly understood LUF syndrome .
| null |
3477_biomrc
|
Title: Intracellular free XXXX in the neutrophils of maintenance haemodialysis @entity1 .
human, patients77 has on occasion been referred to as a state of calcium human, patients37 . The aim of this study was to investigate the status of intracellular free human, patients41 in the neutrophils of human, patients77 human, patients on maintenance haemodialysis treatment. Factors previously suggested to influence intracellular free human, patients41 were investigated including PTH levels, oxidative stress and recombinant human, patients human, patients307 administration. The study involved 14 human, patients77 human, patients on the haemodialysis programme of the Pretoria Academic hospital. Intracellular free human, patients41 and transmembrane human, patients41 fluxes were investigated by fluorescence spectrophotometry. Increases above control values were found in intracellular free human, patients41 (P-value 0.0242) and in the transmembrane human, patients41 flux upon fMLP stimulation (P-value 0.0002). The results showed significant differences in intracellular free human, patients41 between human, patients on rHuEPO and human, patients not on rHuEPO. The apparently rHuEPO-induced increase in intracellular free human, patients41 persisted in the presence of calcium channel blockers. No overt indications of oxidative stress could be detected by the antioxidant vitamin levels. It is concluded that factors other than those associated with uraemia, such as rHuEPO administration, might contribute to the often reported increase in intracellular free human, patients41 in these human, patients . Further studies to investigate the relationship between intracellular free human, patients41 , rHuEPO and calcium channel blockers are suggested.
| null |
1429_biomrc
|
Title: Assessment of novel combinations of biomarkers for the detection of XXXX .
BACKGROUND: Patients, patients with Patients, patients4 often present with Patients, patients442 and concomitant poor prognosis. The best known serum biomarker, CEA, carcinoembryonic antigen ( CEA, carcinoembryonic antigen ) is not recommended for screening because of its limited specificity and sensitivity. A number of other circulating proteins have been suggested to be diagnostically useful but individually none of these has proved to be of sufficient sensitivity or specificity to establish a role in routine clinical practice. Here, we test the hypothesis that combining several of these biomarkers will improve diagnostic efficacy. METHODS: To select the markers for our model we screened CEA, carcinoembryonic antigen and 26 other candidate biomarkers. Four candidates were selected and their concentrations determined in the serum of 239 Patients, patients (106 Patients, patients4 Patients, patients and 133 non- cancers, cancer subjects). RESULTS: Class prediction models based on CEA, carcinoembryonic antigen , DR-70 and sCD26 produced a modest increase in detection accuracy over CEA, carcinoembryonic antigen alone, particularly for early stage cancers, cancer . The sensitivity and specificity required for a clinically useful test was not reached. CONCLUSION: It is unlikely that a biomarker panel comprised of the currently available serum markers will generate a clinically useful diagnostic test for Patients, patients4 . Our findings reiterate the urgent need to discover novel biomarkers for the detection of Patients, patients4 .
| null |
1426_biomrc
|
Title: Destabilization of XXXX liposomes at the hexagonal phase transition temperature.
We have examined whether there is a relationship between the lamellar-hexagonal phase transition temperature, TH, and the initial kinetics of H+- and Ca2+ -induced destabilization of phosphatidylethanolamine (PE) liposomes. The liposomes were composed of dioleoylphosphatidylethanolamine , egg phosphatidylethanolamine (EPE), or phosphatidylethanolamine prepared from egg phosphatidylcholine by transesterification (TPE). These lipids have well-spaced lamellar-hexagonal phase transition temperatures (approximately 12, approximately 45, and approximately 57 degrees C) in a temperature range that allows us to measure the initial kinetics of bilayer destabilization, both below and above TH. The liposomes were prepared at pH 9.5. The TH of EPE and TPE was measured by using differential scanning calorimetry, and it was found that the TH was essentially the same at low pH or at high pH in the presence of 20 mM Ca2+ . At temperatures well below TH, either at pH 4.5 or at pH 9.5 in the presence of Ca2+ , the liposomes aggregate, leak, and undergo lipid mixing and mixing of contents. We show that liposome/liposome contact is involved in the destabilization of the PE liposomes. The temperature dependence of leakage, lipid mixing, and mixing of contents shows that there is a massive enhancement in the rate of leakage when the temperature approaches the TH of the particular PE and that lipid mixing appears to be enhanced. However, the fusion (mixing of aqueous contents) is diminished or even abolished at temperatures above TH. At and above the TH, a new mechanism of liposome destabilization arises, evidently dependent upon the ability of the PE molecules to adapt new morphological structures at these temperatures. We propose that this destabilization demarks the first step in the pathway to the eventual formation of the HII phase. Thus, the polymorphism accessible to PE is a powerful agent for membrane destabilization, but additional factors are required for fusion.
| null |
3482_biomrc
|
Title: @entity148 and XXXX are associated with increased counts and degranulation of duodenal mast cells in @entity2210 .
BACKGROUND: Patients, patients, peoples with functional dyspepsia, FD ( functional dyspepsia, FD ) have increased risks for psychological dysfunction than healthy Patients, patients, peoples . This study aimed to explore the roles of psychosocial factors and duodenal mast cells in the pathogenesis of functional dyspepsia, FD . MATERIAL AND METHODS: We prospectively included 48 functional dyspepsia, FD Patients, patients, peoples and 21 age- and sex-match healthy volunteers. There were 23 Patients, patients, peoples with PDS, postprandial distress syndrome ( PDS, postprandial distress syndrome ) and 25 Patients, patients, peoples with Patients, patients, peoples58 ( Patients, patients, peoples58 ). The Hospital Anxiety Depression, depression Scale (HADS) was administered to evaluate their psychosocial status. Upper endoscopy was performed with biopsy of the mucosa from the bulb of duodenum. Mast cells counts and degranulation rates were identified by Patients, patients, peoples1979 staining. The relationship among the scores of HADS-A ( Patients, patients, peoples48 ) and HADS-D ( Hospital Anxiety Depression, depression ) and the mast cell counts and degranulation rates were analyzed. RESULTS: The scores of HADS-A and HADS-D were significantly higher in PDS, postprandial distress syndrome and Patients, patients, peoples58 Patients, patients, peoples than the normal controls (P<0.05). The mast cell counts and degranulation rates in the duodenum were significantly increased in PDS, postprandial distress syndrome and Patients, patients, peoples58 Patients, patients, peoples than the controls (P<0.05). In either PDS, postprandial distress syndrome or Patients, patients, peoples58 Patients, patients, peoples , the HADS-A and HADS-D scores were positively correlated with the mast cell counts and degranulation rate. CONCLUSION: functional dyspepsia, FD Patients, patients, peoples had significantly higher risks for Patients, patients, peoples48 and Hospital Anxiety Depression, depression , which may lead to functional dyspepsia, FD through the increased mast cell counts and degranulation.
| null |
1435_biomrc
|
Title: XXXX : an essential part of lower gastrointestinal endoscopic examination.
PURPOSE: Retroflexion of the endoscope during rectal examination may increase diagnostic yield but is not routinely performed because of concerns about safety and a lack of appreciation of its importance. The purpose of this study was to examine the yield, safety, and tolerance of endoscopic rectal retroflexion . METHODS: Prospective cohorts of subjects undergoing unsedated screening flexible sigmoidoscopy were examined with and without routine retroflexion. Pain, pain scores were recorded. RESULTS: A total of 526 subjects (mean age 60 (range, 55-66) years) underwent flexible sigmoidoscopy in the first period when the endoscope was not routinely retroflexed. Of these, 480 (mean age 60 (range, 55-66) years) were subsequently examined with routine retroflexion. Retroflexion was impossible in 17 subjects (3.5 percent) because of discomfort. In the second group, 12 subjects (2.5 percent) had polyps in the lower rectum seen only on retroflexion. Of these, eight had metaplastic and four had adenomatous polyps (3 tubular <5 mm, 1 tubulovillous 15 mm). There was no difference in mean Pain, pain scores between the groups (no retroflexion = 2.13, retroflexion = 2.18). CONCLUSION: With an adenoma pick-up rate of 8 to 12 percent for screening flexible sigmoidoscopy, retroflexion increases adenoma detection by approximately 1 percent without adverse effects and should be an integral part of flexible sigmoidoscopy.
| null |
1434_biomrc
|
Title: Differential reactivity of rheumatoid synovial cells and serum rheumatoid factors to XXXX immunoglobulin G subclasses 1 and 3 and their CH3 domains in @entity309 .
19S IgM rheumatoid factors (RF) are polyclonal autoantibodies that may play an important pathogenic role in sustaining inflammatory synovitis in rheumatoid arthritis, RA ( rheumatoid arthritis, RA ). RF in rheumatoid arthritis, RA have reactivity for as-yet-uncharacterized antigenic determinants in IgG Fc. We hypothesized that qualitative differences might exist between some of these RF molecules, and that differences such as reactivity and affinity might characterize more pathogenic RF molecules. Previous observations in our laboratory indicate that RF produced by rheumatoid synovial cells (RSC) have greater reactivity with human IgG and IgG3 subclass, in contrast to serum RF, which has greater reactivity with rabbit IgG and human IgG1. These observations were made using a complement-dependent RF plaque-forming cell assay. The purpose of this study was to validate and extend those observations. Therefore, we examined the reactivity of RSC and serum RF with human and rabbit IgG and the reactivity and avidity of RSC-RF for IgG1 and IgG3 molecules and human334 , F(ab')2, and pFc' fragments thereof in a solid-phase enzyme immunoassay. In particular, we found: RSC-RF had at least twice as much reactivity with human IgG as with rabbit IgG; serum RF had approximately equal reactivity with human and rabbit IgG; RSC-RF had greater reactivity and avidity for IgG3 and IgG3 pFc' than for IgG1; and RSC-RF was nonreactive with human334 or F(ab')2 from either IgG1 or IgG3 . These results suggest that the major antigenic determinant for RSC-RF resides in the CH3 domain of the IgG3 molecule. Precise characterization of this epitope may provide further insight into the etiology and pathogenesis of rheumatoid arthritis, RA .
| null |
1440_biomrc
|
Title: Identification of XXXX bacteria isolated from South African brandy base wines.
In brandy base wines, no sulphur dioxide is used and it therefore is ideal for the proliferation of lactic acid bacteria. As part of an extensive taxonomic survey within the ecological framework of South African vineyards and wineries, and the influence of naturally occurring lactic acid bacteria on the quality of wine and brandy, a total of 54 strains were isolated from grape juice and at different stages of brandy base wine production. The strains were identified using numerical analysis of total soluble cell protein patterns, 16S rRNA sequence analyses and polymerase chain reaction (PCR) using species-specific primers. The predominant species was Oenococcus oeni, O. oeni (22 strains), but Lactobacillus brevis (8 strains), Lactobacillus paracasei (8 strains) and Lactobacillus, Lactobacillus plantarum (6 strains) were also isolated frequently. Many of the Oenococcus oeni, O. oeni strains were isolated from brandy base wines after completion of spontaneous malolactic fermentation (MLF). The Lactobacillus, Lactobacillus plantarum spp. were isolated from all the different stages of brandy base wine production. Lb. plantarum was the dominant species in the juice, but disappeared during the later stages of production. However, Lactobacillus, Lactobacillus plantarum hilgardii, Lb. brevis and Lb. paracasei were also isolated from base wine after spontaneous MLF. Strains identified as Lactobacillus, Lactobacillus plantarum vermiforme were isolated during the alcoholic fermentation and after MLF have been completed. Total soluble cell protein patterns grouped Oenococcus oeni, O. oeni strains into two phenotypic groups. Two phenotypic clusters have also been identified for the Lb. brevis isolates. The Lb. paracasei isolates all grouped in one cluster. This is the first report of the presence of Lb. paracasei and Lb. vermiforme in brandy base wines. The presence of the Lactobacillus, Lactobacillus plantarum spp. could be correlated to the decrease in quality of the base wine and distillate, while Oenococcus oeni, O. oeni strains were found to have a more favourable influence on the quality of base wine and distillates. These results shed some light on the ecology and oenological influence of lactic acid bacteria (LAB) on the quality of South African brandy.
| null |
3490_biomrc
|
Title: Effects of exogenous @entity5445 on blood pressure, heart rate, gastric emptying, mesenteric blood flow and glycaemic responses to oral @entity413 in older individuals with normal XXXX tolerance or @entity164 2 @entity6 .
AIMS/HYPOTHESIS: A postprandial fall in BP occurs frequently in older individuals and in women, patients, men with women, patients, men64 2 diabetes . The magnitude of this decrease in BP is related to the rate of gastric emptying (GE). Intravenous administration of glucagon-like peptide-1, GLP-1 ( glucagon-like peptide-1, GLP-1 ) attenuates the hypotensive, hypotension response to intraduodenal glucose in healthy older individuals. We sought to determine the effects of exogenous glucagon-like peptide-1, GLP-1 on BP, GE, diabetes029 ( diabetes029 ) flow and glycaemic response to oral ingestion of glucose in healthy older individuals and women, patients, men with women, patients, men64 2 diabetes . METHODS: Fourteen older volunteers (six women, patients, men , eight women, patients, men ; age 72.1 1.1 years) and ten women, patients, men with women, patients, men64 2 diabetes (six women, patients, men , four women, patients, men ; age 68.7 3.4 years; HbA1c 6.6 0.2% [48.5 2.0 mmol/mol]; nine with blood glucose managed with metformin , two with a sulfonylurea and one with a dipeptidyl-peptidase 4 inhibitor) received an i.v. infusion of glucagon-like peptide-1, GLP-1 (0.9 pmol kg(-1) min(-1)) or saline (154 mmol/l NaCl ) for 150 min (t = -30 min to t = 120 min) in randomised order. At t = 0 min, volunteers consumed a radiolabelled 75 g glucose drink. BP was assessed with an automated device, GE by scintigraphy and diabetes029 flow by ultrasonography. Blood glucose and serum insulin were measured. RESULTS: glucagon-like peptide-1, GLP-1 attenuated the fall in diastolic BP after the glucose drink in older individuals (p < 0.05) and attenuated the fall in systolic and diastolic BP in women, patients, men with women, patients, men64 2 diabetes (p < 0.05). GE was faster in women, patients, men with women, patients, men64 2 diabetes than in healthy individuals (p < 0.05). In both groups, individuals had slower GE (p < 0.001), decreased diabetes029 flow (p < 0.05) and a lower degree of glycaemia (p < 0.001) when receiving glucagon-like peptide-1, GLP-1 . CONCLUSIONS/INTERPRETATION: Intravenous glucagon-like peptide-1, GLP-1 attenuates the hypotensive, hypotension response to orally administered glucose and decreases diabetes029 flow, probably by slowing GE. glucagon-like peptide-1, GLP-1 and 'short-acting' glucagon-like peptide-1, GLP-1 agonists may be useful in the management of postprandial hypotensive, hypotension .
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3492_biomrc
|
Title: Soft tissue XXXX after direct laryngoscopy.
STUDY OBJECTIVE: The study objective is to determine the incidence of oral soft tissue patients, patient, PATIENTS30 during classic direct laryngoscopy for tracheal intubation and the risk factors associated with it. DESIGN: This is a prospective observational study. SETTING: The setting is at a ward. patients, patient, PATIENTS : The patients, patient, PATIENTS are adults submitted to elective interventions in general surgery requiring tracheal intubation by classic direct laryngoscopy. INTERVENTIONS: During 6 months, all patients, patient, PATIENTS were interviewed 12-24 hours before anesthesia and after surgery and underwent a detailed oral examination performed by an anesthesiology blind to anesthetic management details and preoperative patients, patient, PATIENTS care. MEASUREMENTS: Evaluation of oral soft tissue patients, patient, PATIENTS30 includes oral mucosa including the gums; the patients, patient, PATIENTS33 in the edentulous patients, patient, PATIENTS , oral mucosa injury, palate, and the buccal mucosa; lips (mucosa and skin); and the tongue . Injury severity was assessed using the severity scale presented routinely in Portuguese legal medicine research: grade 0, no patients, patient, PATIENTS30 had; grade 1, mild severity patients, patient, PATIENTS30 ; grade 2, medium severity patients, patient, PATIENTS30 ; and grade 3, major severity patients, patient, PATIENTS30 . MAIN RESULTS: Soft tissue patients, patient, PATIENTS30 was observed in 278 (52.1%) patients, patient, PATIENTS . Soft tissue injury occurred once in 204 (38.2%) patients, patient, PATIENTS , 2 in 64 (38.2%) patients, patient, PATIENTS , and 3 times in 10 (1.9%) patients, patient, PATIENTS . Tongue injury was the most common type of soft tissue patients, patient, PATIENTS30 (36.3%) followed by upper lip injury, lower lip injury (22.3%), upper lip injury, lower lip injury (7.1%), and oral mucosa injury, palate, and the buccal mucosa; lips (mucosa and skin); and the tongue (2.1%). All the lesions were grade 1 or 2. Only oral mucosa injury, palate, and the buccal mucosa; lips (mucosa and skin); and the tongue was found to be associated with age group (P = .021). CONCLUSIONS: Our study reveals a high incidence of lesions grade 1 or 2 in soft tissue.
| null |
3495_biomrc
|
Title: Clinically acceptable colchicine concentrations have potential for the palliative treatment of @entity1 XXXX .
Microtubules are an ideal target for anticancer drugs because of their essential role in mitosis. Colchicine is a microtubule destabilizer. Whether the clinically acceptable colchicine concentrations had anticancer effects on human cholangiocarcinoma cells was investigated. Two human cholangiocarcinoma cell lines (C14/KMUH, C51/KMUH) were investigated using clinically acceptable plasma colchicine concentrations (2 ng/mL and 6 ng/mL for the in vitro experiment, 0.07 mg colchicine/kg/d * 14 days for the nude human9 experiment). Our results showed that colchicine caused significantly dose-dependent antiproliferative effects on both cell lines (all p < 0.0001). Nude human9 (BALB/c-nu) experiments showed that the increased tumor volume ratios in colchicine-treated human9 were significantly lower than control human9 started from the 11th day of treatment (p = 0.0167). The tumor growth rates in colchicine-treated human9 after 14 days of treatment were significantly lower than in control human9 (0.147 0.004/d vs. 0.274 0.003/d, p = 0.0015). In addition to the well-known direct colchicine-tubulin interaction as a common anticancer mechanism of colchicine, microarray and quantitative reverse transcriptase-polymerase chain reaction showed that the antiproliferative effects of both 2 ng/mL and 6 ng/mL colchicine on C14/KMUH cells could be partially explained by downregulations of both tumor6811 and MT-COI. There was no effect of colchicine on MT-COI expression in C51/KMUH cells, however, 6 ng/mL colchicine also downregulated tumor6811 in this cell line. In conclusion, clinically acceptable colchicine concentrations can inhibit the proliferation of human cholangiocarcinoma cells. This drug has good potential for the palliative treatment of cholangiocarcinoma due to its low cost and our long-standing prescription experience.
| null |
1451_biomrc
|
Title: XXXX and hepatosteatosis in @entity19 with enhanced oxidative DNA damage processing in mitochondria.
Mitochondria play critical roles in oxidative phosphorylation and energy metabolism. Increasing evidence supports that mitochondrial DNA (mtDNA) damage and dysfunction play vital roles in the development of many mitochondria-related diseases, such as Obesity, obesity , diabetes mellitus , infertility , humans, human, Human79 , and malignant tumors, malignancy in humans, human, Human . humans, human, Human 8-oxoguanine -DNA glycosylase 1 ( hOGG1 ) transgenic (TG) humans, human, Human9 were produced by nuclear microinjection. Transgene integration was analyzed by PCR. Transgene expression was measured by RT-PCR and Western blot analysis. Mitochondrial DNA damage was analyzed by mutational analyses and measurement of mtDNA copy number. Total fat content was measured by a whole-body scan using dual-energy X-ray absorptiometry. The hOGG1 overexpression in mitochondria increased the abundance of intracellular free radicals and major deletions in mtDNA. Obesity, obesity in hOGG1 TG humans, human, Human9 resulted from increased fat content in tissues, produced by hyperphagia . The molecular mechanisms of Obesity, obesity involved overexpression of genes in the central orexigenic (appetite-stimulating) pathway, peripheral lipogenesis, down-regulation of genes in the central anorexigenic (appetite-suppressing) pathway, peripheral adaptive thermogenesis, and fatty acid oxidation. Diffuse hepatosteatosis, female infertility , and increased frequency of malignant lymphoma were also seen in these hOGG1 TG humans, human, Human9 . High levels of hOGG1 expression in mitochondria, resulting in enhanced oxidative DNA damage processing, may be an important factor in humans, human, Human metabolic syndrome , infertility , and malignant tumors, malignancy .
| null |
3503_biomrc
|
Title: The current status of suicide and self- XXXX : a narrative review.
The aim of this paper is to review recent literature on suicide and self- patients277 ( patients277 ) including patients278 ( patients278 ), patients279 ( patients279 ), and BED, binge eating disorder ( BED, binge eating disorder ). Among psychiatric diagnoses, EDs are associated with increased mortality rates, even when specialized treatment is available. Of the mortalities that are reported in individuals with EDs , suicide is among the most commonly reported causes of death . Additionally, suicidal and non-suicidal self-injurious behaviors occur frequently in this clinical population. A literature search was undertaken using the databases of Medline/PubMed and PsycInfo to identify papers describing suicidality in individuals with patients277 diagnoses. The authors identified studies and review articles published between 2005-2013 (inclusive) that describe the relationship between EDs and suicide, and associated behaviors including self-injurious behaviors, or patients30 ( patients30 ). The initial search resulted in 1095 papers that met the a priori search criteria. After careful review, 66 papers were included. The majority of papers described clinical cohorts that were studied longitudinally. The diagnosis described most frequently in selected studies was patients278 . There are limited current data about the prevalence of suicide and patients30 among individuals with EDs . Among the published studies that focus specifically on the relationship between EDs and suicidality, most describe patients278 in more detail than other EDs . Nonetheless, rates of mortality, and specifically rates of suicide, are undeniably high in patients277 populations, as are the rates of self-harm. Therefore, it is critical for clinicians and caretakers to carefully evaluate these patients for suicide risk and to refer promptly for appropriate treatment.
| null |
3509_biomrc
|
Title: Detection of XXXX -associated @entity50070 translocation early during chemotherapy with DNA topoisomerase II inhibitors.
child778 with child1626 gene translocations are a complication of primary cancer treatment with DNA topoisomerase II inhibitors. How early translocations appear during primary cancer treatment has not been investigated. We tracked the leukemic clone with an child1626 gene translocation during cancer21 therapy in a child who developed acute myeloid leukemia . The karyotype of the leukemic clone showed del(11)(q23). We used panhandle PCR-based methods to isolate the breakpoint junction involving child1626 and an unknown partner gene. Marrow DNA from cancer21 diagnosis and DNA and RNA from serial preleukemic marrows were examined for the translocation. The karyotypic del(11)(q23) was a cryptic t(11;17). cancer0070 , a growth arrest-specific gene at chromosome band 17p13, was the partner gene of child1626 . Two different cancer0070 fusion transcripts were expressed. The translocation was already detectable by 1.5 months after the start of cancer21 treatment. The translocation was not detectable in the marrow at cancer21 diagnosis or in peripheral blood lymphocyte DNAs of six normal subjects. cancer0070 is a new partner gene of child1626 in treatment-related acute myeloid leukemia . child1626 gene translocations can be present early during anticancer treatment at low cumulative doses of DNA topoisomerase II inhibitors. Although child1626 has many partner genes and most have not been characterized, panhandle PCR strategies afford new means for detecting child1626 gene translocations early during therapy when the partner gene is unknown.
| null |
1462_biomrc
|
Title: Integration of XXXX -DNA may not be a prerequisite for the maintenance of the state of malignant transformation. An analysis of 110 liver biopsies.
Hundred and ten liver biopsy specimens from various parts of the world were examined for episomal and integrated HBV -DNA sequences. In 54 patients with HBsAg chronic liver disease episomal HBV -DNA was found in 83% of HBeAg-positive patients , compared to only 22% of patients with anti-HBe. Furthermore episomal HBV -DNA in the latter predominated among the Asians. Integrated HBV -DNA was found only in 5.5% of HBeAg-positive patients but in 16.5% of patients with anti-HBe. In 28 HBsAg-positive patients with patients57 , episomal HBV -DNA was found in 50% of HBeAg-positive patients but in only 11% of anti-HBe patients . Conversely integrated sequences were less common (25%) in HBe-Ag-positive patients than in anti-HBe patients (50%), giving an overall incidence of integration in this group of 45%. No episomal, and only one case of integrated sequences of HBV -DNA, could be detected among 10 patients with patients57 . In addition neither episomal nor integrated HBV -DNA could be detected in 18 patients with non- HBV -related liver disease . Our data suggests that stable integration of HBV -DNA into the host's genome is not necessarily a prerequisite for the maintenance of the state of malignant transformation but may be necessary for its initiation. Alternatively, the detection of integrated HBV -DNA may represent a 'snap shot' of a random integration event amplified by clonal expansion promoted by other factors.
| null |
1463_biomrc
|
Title: A dynamic smoke generation and nose-only inhalation exposure system for XXXX : preliminary results from studies of selected transportation materials.
Abstract Context: Smoke human30 is the main cause of fatalities for fire victims. Understanding in the pathophysiology of the injury has not been fully explored in recent years. To further explore the pathophysiological mechanism, a dynamic and controllable animal model is necessary. OBJECTIVE: To develop a rat, rats model of smoke inhalation injury to simulate human victims in air-restricted vehicle cabin fires. MATERIALS AND METHODS: Smoke concentration, including CO, O2, VOCs and smoke temperature under different combustion conditions, were detected. Levels of COHb, respiratory function, lung wet-to-dry weight ratio and protein concentration in BALF and blood were measured. Pathological evaluations of lung in tissues were conducted at 1, 6, 24 and 48 h post-exposure. RESULTS: Smoke concentration rose with the increase of combustion temperature and decrease of oxygen flow. Further, 215 kinds of VOCs in the smoke were detected, and the concentrations of benzene , methylbenzene , human8008 , dimethylbenzene , phenylethylene and trimethylbenzene was 32.93, 402.06, 764.03, 113.73, 1006.61 and 89.28 mg/m(3), respectively. Significant hypoxemia and lung edema968 occurred in rat, rats . The FCOHb after exposure for 14 min immediately rose to (44.2 12.3) % and then gradually decrease to a normal level at 300 min post-exposure. At 24 h post-exposure, Penh increased significantly (p < 0.05), and high pulmonary vascular permeability and significant lung edema (p < 0.05) were observed in the smoke inhalation group. DISCUSSION AND CONCLUSION: In summary, the novel rat, rats model of smoke inhalation injury system used in the study is dynamic and controllable, and appropriate for use in smoke inhalation injury studies of air-restricted cabins in vehicles.
| null |
3512_biomrc
|
Title: Immunological and XXXX of risk status and illness development in high-risk youth: understanding the neurobiological underpinnings of @entity162 .
bipolar disorder, Bipolar disorder, bipolar parents is a highly heritable illness that onsets in adolescence and young adulthood. We examined gene expression (mRNA) and protein levels of candidate immune and neurotrophic markers in well-characterized offspring of bipolar disorder, Bipolar disorder, bipolar parents in order to identify reliable indicators of illness risk status and the early clinical stages of illness development. We measured mRNA expression and protein levels in candidate immune ( TNF-a , IL-1b , IL-10, IFN- ) and neurotrophic ( brain-derived neurotrophic factor, BDNF ( brain-derived neurotrophic factor, BDNF )) markers from plasma. High-risk offspring were identified from families in which one parent had confirmed bipolar disorder, Bipolar disorder, bipolar parents . Control offspring were identified from families in which neither parent met lifetime criteria for a major Mental Disorders, psychiatric disorder . All parental Diagnostic and Statistical Manual of Mental Disorders, psychiatric disorder , Fourth Edition (DSM-IV) diagnoses were based on Schedule for Affective Disorders - Lifetime Version (SADS-L) interviews and blind consensus review. As part of an ongoing study, all offspring were prospectively assessed using KSADS-PL format interviews and diagnoses confirmed on blind consensus review. High-risk offspring had significantly increased IL-6 (p = 0.050) and brain-derived neurotrophic factor, BDNF (p = 0.006) protein levels compared to controls. Those high-risk offspring in earlier compared to later clinical stages of illness development had higher IL-6 (p = 0.050) and brain-derived neurotrophic factor, BDNF (p = 0.045) protein levels. After adjustments, only differences in brain-derived neurotrophic factor, BDNF protein levels remained significant. There was a moderating effect of the brain-derived neurotrophic factor, BDNF genotype on both gene expression and protein levels in high-risk compared to control offspring. The brain-derived neurotrophic factor, BDNF genotype also moderated the association between clinical stage and gene expression levels in high-risk offspring. These findings provide support for detectable differences in candidate immune and neurotrophic markers in individuals at high risk of developing bipolar disorder, Bipolar disorder, bipolar parents and for detectable changes over the clinical stages of illness development. These associations appear to be moderated by genetic variants.
| null |
3515_biomrc
|
Title: Average daily dose, medication adherence, and healthcare costs among commercially-insured @entity1 with @entity239 treated with XXXX .
OBJECTIVE: What is the relationship between ADD, average daily dose ( ADD, average daily dose ) of duloxetine, Duloxetine , adherence to therapy, and healthcare costs among Patients, patients with Fibromyalgia, fibromyalgia ? METHODS: Employing a retrospective cohort design, this study assessed administrative claims data for commercially-insured Fibromyalgia, fibromyalgia Patients, patients who initiated duloxetine, Duloxetine in 2006. Initiation was defined as a 90-day clean period without access to duloxetine, Duloxetine . Five study cohorts were constructed based on the ADD, average daily dose of duloxetine, Duloxetine , calculated from all prescriptions dispensed over the 12-month follow-up period: <30 mg, 30 mg, 31-59 mg, 60 mg, and >60 mg ( duloxetine, Duloxetine starting dose: 30 mg/day; target dose: 60 mg/day). Medication possession ration (MPR) was used to assess duloxetine, Duloxetine adherence, with MPR >= 0.8 as 'high' adherence. Multivariate regressions were applied to examine the association between ADD, average daily dose , duloxetine, Duloxetine adherence, and healthcare costs (total, inpatient, outpatient, and pharmacy), adjusting for differences in demographic and clinical characteristics between cohorts. RESULTS: Of the 4869 Patients, patients with Fibromyalgia, fibromyalgia , 3% had an ADD, average daily dose of <30 mg, 12% had 30 mg, 21% had 31-59 mg, 46% had 60 mg, and 18% had >60 mg. High adherence to duloxetine, Duloxetine ranged from 16% to 41% across cohorts. Controlling for demographic and clinical characteristics, Patients, patients with an ADD, average daily dose of 31-59 mg or >60 mg were more likely to adhere to duloxetine, Duloxetine therapy than those in the 60 mg cohort, while Patients, patients in the <30 mg cohort were less likely to adhere to duloxetine, Duloxetine (all p < 0.05). Total costs were significantly higher for individuals in the 31-59 mg or >60 mg ADD, average daily dose cohorts ( 1834 and 5766) than those maintaining an ADD, average daily dose of 60 mg, as were outpatient ( 1167 and 2275) and pharmacy costs ( 502 and 2983) (all p < 0.05). Patients, patients with an ADD, average daily dose of 30 mg had significantly lower total (- 1770), inpatient (- 1524), and pharmacy (- 827) costs (all p < 0.05) than those with an ADD, average daily dose of 60 mg. LIMITATIONS: This study is subject to selection bias because some confounders might be unobserved in the administrative claims database. Indirect costs or over-the-counter medication costs were also not available. CONCLUSIONS: Fibromyalgia, fibromyalgia Patients, patients with an ADD, average daily dose of 60 mg of duloxetine, Duloxetine had better adherence than Patients, patients with an ADD, average daily dose <=30 mg. duloxetine, Duloxetine Patients, patients with an ADD, average daily dose of 30 mg (31-59 or >60 mg) had significantly lower (higher) healthcare costs than those maintaining an ADD, average daily dose of 60 mg.
| null |
3516_biomrc
|
Title: Combined @entity231 surgery and XXXX ring implantation in @entity16392 .
PURPOSE: To present results of cataract surgery combined with aniridia ring implantation in patients with patients6392 ( patients6392 ) in terms of vision, refraction, eye movements, and subjective photophobia and glare. SETTING: Anterior segment services of Noor Eye Hospital, Tehran, Iran. DESIGN: Consecutive clinical case series study. METHODS: Enrolled patients underwent phacoemulsification and posterior chamber intraocular lens (PC IOL) implantation and had 2 Morcher aniridia rings (types 50 F or 50 E) inserted intracapsularly. Preoperative and postoperative examinations included tests for visual acuity, manifest refraction, measurement of ocular alignment, and electronystagmography. After surgery, patients also completed a questionnaire to subjectively assess glare reduction. RESULTS: Twelve eyes of 6 patients6392 patients were included. Minimum follow-up was 6 months. None of the patients had any intraoperative or postoperative complications . In addition to improved uncorrected and corrected distance visual acuity and significant reduction of refractive error , all patients had a marked reduction of glare and photophobia after surgery. CONCLUSION: Iris ring implantation during cataract surgery was an effective procedure for increasing vision and reducing glare disability in patients with patients6392 . In light of the results and patients ' expressed satisfaction, a study should be performed to assess the outcomes of refractive lens exchange along with implantation of a PC IOL and prosthetic iris device in patients6392 patients seeking refractive correction.
| null |
1469_biomrc
|
Title: Low-dose UVB radiation perturbs the functional expression of @entity12750 co-stimulatory molecules on XXXX Langerhans cells.
In previous studies, we have shown that ultraviolet (UV) B radiation perturbs the human412 function of Langerhans cells (LC) by interfering with as-yet unidentified co-stimulatory signals. Recently, human2750 on human412 were shown to deliver important co-stimulatory signals through interaction with their counter receptors human361 and CTLA-4 on T cells. To determine whether UVB affects the functional expression of B7.1 or B7.2 on LC, human2750 expression was studied on human LC by multiparameter flow cytometry. Little, if any, B7.1 or B7.2 was detected on LC freshly isolated from skin. However, following 48 h of tissue culture, expression of both human2750 were markedly up-regulated. To test whether these molecules were functional, primary mixed epidermal cell leukocyte reactions (MECLR) were performed. Blocking monoclonal antibody (mAb) to B7.1 or B7.2 both inhibited the MECLR, with anti- B7.2 being much more effective than anti- B7.1 . UVB radiation dose-dependently (100-200 J/m2) suppressed the culture-induced up-regulation of B7.1 and B7.2 on LC. Since LC exposed to the same UVB flux (UVB-LC) failed to stimulate alloreactive T cells in a MECLR, we questioned whether this was related to their inability to provide B7 co-stimulation. Indeed, when effective B7- human361 signaling was ascertained by adding submitogenic doses of exogenous anti- human361 mAb to UVB-LC, the proliferative response of alloreactive T cells was restored. We conclude that the suppressive effects of low-dose UVB radiation on the human412 function of LC are, at least in part, due to an inhibition of functional human2750 expression.
| null |
3517_biomrc
|
Title: Polymorphisms in XXXX gene ( @entity7742 and G(-6)A) in @entity66 .
The aim of the study is to compare results of three association (case-control) studies in three multifactorial disorders ( women, patients, men01 , atopic diseases and women, patients, men569 ) with two polymorphisms of angiotensinogen gene ( M235T and A(-6)G). The diseases were chosen for their multigenic base and different immunological characteristic ( women, patients, men198 , Th2 and Thps) and angiotensinogen gene for its pleiotropic functional effects in general adaptive reactions. In all (control as well as case) groups, tight linkage disequilibrium between the polymorphisms was found. The strength of linkage (%) differed among the group. The direction of the linkage is identical in all groups (T is combined with A, M is combined with G). In women, patients, men01 -normotensive study, only Hardy-Weinberg disequilibria were found, especially in women, patients, men . No case-control differences were found for either single alleles or for allelic concurrence of both polymorphisms. In atopy-control study, marginal case-control differences in single allele distribution of both polymorphisms were found, but only in women, patients, men . In women, patients, men569 -control study, the only significant case-control difference was found,when genotypes MTAA and MTGG were present in 2/136 psoriatic women, patients, men vs. 20/142 control subjects (OR 0.1, 95% confidence interval 0.02-0.42, P=0.00015). The frequent polymorphisms in pleiotropic genes can form different formulae of genotype distribution in different multigenic diseases according to their contribution to the onset and/or progression of the disease in some evolutionary consequences.
| null |
3519_biomrc
|
Title: The associated regulators and signal pathway in @entity66314 / XXXX mediated growth regulation in Jurkat cells.
IL-16 is a ligand and chemotactic factor for CD4 + T cells. IL-16 inhibits the CD3 mediated lymphocyte activation and proliferation. The effects of IL-16 on the target cells are dependent on the cell type, the presence of co-activators etc. To understand the regulation function and mechanism of IL-16 on target cells, we used a 130 a.a. recombinant IL-16 to study its effects on the growth of Jurkat T leukemia cells in vitro. We found that the rIL-16 stimulated the proliferation of Jurkat cells at low dose (10(-9)M), but inhibited the growth of the cells at higher concentration (10(-5)M). Results showed that 10(-5) M of rIL-16 treatment induced an enhanced apoptosis in Jurkat cells. The treatment blocked the expression of FasL , but up-regulated the c-myc, c-Myc and Bid expression in the cells. Pre-treatment of PKC inhibitor or FasL363 inhibitor markedly increased or decreased the rIL-16 induced growth-inhibiting effects on Jurkat cells, respectively. The results suggested that the rIL-16 might be a regulator for the growth or apoptosis of Jurkat cells at a dose-dependent manner. The growth-inhibiting effects of rIL-16 might be Fas/ FasL independent, but, associated with the activation of PKC , up-regulated expression of c-myc, c-Myc and Bid , and the participation of the ERK signal pathway in Jurkat cells.
| null |
3521_biomrc
|
Title: Determination of a Degradation Constant for XXXX by Direct Suppression of mRNA in a Novel @entity1 Hepatocyte Model, HepatoPac .
UNASSIGNED: Accurate determination of rates of de novo synthesis and degradation of cytochromes human1670 ( human1670 ) has been challenging. There is a high degree of variability in the multiple published values of turnover for specific P450s which is likely exacerbated by differences in methodologies. For CYP3A, CYP3A4 , half-life values range from 10 to 140h (Yang et al., 2008). An accurate value for kdeg has been identified as a major limitation for prediction of drug interactions involving mechanism based inhibition and/or induction. Estimation of human1670 half-life from in vitro test systems, such as human hepatocytes, is complicated by differential decreased enzyme function over culture time, attenuation of the impact of enzyme loss through inclusion of glucocorticoids in media, and viability limitations over long-term culture times. HepatoPac overcomes some of these challenges providing an extended stability of enzymes (2.5 weeks in our hands). As such it is a unique tool for studying rates of enzyme degradation achieved through modulation of enzyme levels. CYP3A, CYP3A4 mRNA levels were rapidly depleted by >90% using either siRNA or addition of IL6 which allowed an estimation of the degradation rate constant for CYP3A, CYP3A4 protein over an incubation time of 96 hours. The degradation rate constant of 0.0240 0.005 h(-1) was reproducible in hepatocytes from five different human donors. These donors also reflected the overall population with respect to CYP3A5 genotype. This methodology can be applied to additional enzymes and may provide a more accurate in vitro derived kdeg value for predicting clinical DDI outcomes.
| null |
3530_biomrc
|
Title: Diagnosis of pelvic lymph node @entity3 in XXXX using single optical fiber probe.
UNASSIGNED: Elastic light single-scattering spectroscopy system (ELSSS) is a biomedical tool which is used for detection of cancerous tissues ex-vivo. ELSSS spectra depend primarily on the size of scatterers in the tissue and are not directly related to changes in the absorption which are caused by variations of the biological macromolecules. In the present study, we aimed to detect metastasis in the pelvic lymph node by using combination of Principal Components Analysis (PCA) and patients650 ( patients650 ). Single-scattering spectra in the 450-750nm wavelength regions were obtained from the total of 83 reactive lymph node and 12 metastatic lymph node samples from 10 prostatic cancer, prostate cancer patients . The ELSSS spectral data were compared against the "gold standard" histopathology results. Data analyses were done via using PCA, followed by patients650 . Receiver Operating Characteristic (ROC) curve analysis was employed for differentiating performance. The classification based on discriminant score provided sensitivity of 100% and specificity of 96.4%, in differentiating non-metastatic (reactive) from metastatic pelvic lymph nodes, with a Positive Predictive Value ( PPV ) of 0.8, a Negative Predictive Value (NPV) of 0.99 and the area under the ROC curve (AUC) of 0.99, respectively. In this study, it was shown that ELSSS system can accurately distinguish reactive and metastatic pelvic lymph nodes of prostatic cancer, prostate cancer with high PPV and NPV. It can be concluded that diagnostic accuracy of ELSSS system allows detecting metastatic tissues during operation.
| null |
3539_biomrc
|
Title: The efficacy of intratumoural XXXX for the treatment of @entity1641 sarcoids.
OBJECTIVE: To document the efficacy of intratumoural injections of 5-fluorouracil for the treatment of equine, horses sarcoids. DESIGN: A prospective study that included 13 equine, horses and one donkey . PROCEDURE: Sarcoids were confirmed by histological examination and treated with intratumoural 5-fluorouracil every 2 weeks. If the sarcoids did not resolve after seven treatments, treatment was considered a failure. All cases were re-examined 6 months after treatment commenced and owners were telephoned 3 years after commencement of treatment to report on mutiple tumours, tumours, tumour recurrence. Outcome comparisons were performed to determine the effect of previous treatment, mutiple tumours, tumours, tumour size and mutiple tumours, tumours, tumour location on sarcoid resolution. The efficacy of intratumoural 5-fluorouracil was compared with other previously documented treatments of equine, horses sarcoids. RESULTS: Sarcoids smaller than 13.5 cm3 were significantly (P = 0.032) more likely to resolve with treatment than larger sarcoids. Sarcoids that were not responsive to previous therapies were significantly (P = 0.007) more likely to recur after 3 years than sarcoids that had not been treated prior to this study. In this study, there were similar rates of resolution in cases with mutiple tumours, tumours, tumour (66.6%) when compared to cases with single mutiple tumours, tumours, tumour (60%). The numbers in this study were too small to properly evaluate the effect of mutiple tumours, tumours, tumour location on the success of treatment. Intratumoural 5-fluorouracil appeared to have resolved sarcoids in 9 of 13 cases (61.5%) as determined by follow up conversation with the owners 3 years after the initial treatment. CONCLUSION: The use of intratumoural 5-fluorouracil compares favourably with other treatment modalities for sarcoids, with a long term successful resolution rate of 61.5%. Owners should be warned that resistant sarcoids and sarcoids larger than 13.5 cm3 have a poorer prognosis for resolution and more aggressive therapeutic options should be considered.
| null |
1499_biomrc
|
Title: Optimizing surgical management of symptomatic solitary XXXX .
Four women, patients, patient, men and six women, patients, patient, men with symptomatic solitary hepatic lobectomy, hepatic cysts were encountered over an 11-year period. Average age was 49 years (range, 10 months to 67 years). Most common clinical features included women, patients, patient, men58 , a palpable RUQ mass and early postprandial satiety. Liver function studies were uniformly normal. Ultrasound and computerized tomography CT were virtually diagnostic alone or in combination. Eight women, patients, patient, men had unilocular, simple cysts and two women, patients, patient, men had multilocular complex cysts also designated as biliary cystadenomas, biliary cystadenoma . Cysts recurred in four women, patients, patient, men treated surgically by needle aspiration, incision, and internal drainage or external catheter drainage. All four women, patients, patient, men required reoperation, which included hepatic lobectomy, hepatic cysts in three women, patients, patient, men and extensive unroofing in one women, patients, patient, men . No cysts have recurred in six women, patients, patient, men treated by extensive unroofing or excision of the cyst and none has required reoperation. One women, patients, patient, men who underwent hepatic lobectomy, hepatic cysts for cyst recurrence after an inadequate primary procedure was found to have an unsuspected women, patients, patient, men89 in the cyst wall. The conclusion is that extensive unroofing of unilocular solitary hepatic lobectomy, hepatic cysts minimizes the likelihood of cyst recurrence and obviates the need for hepatic resection. Total cyst excision is indicated for all multiloculated cysts to avoid overlooking a biliary cystadenomas, biliary cystadenoma or for unilocular cysts if the biopsy specimen of the cyst wall demonstrates an unsuspected neoplasm .
| null |
3548_biomrc
|
Title: A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in XXXX .
Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase), ParB protein (a DNA-binding element) and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity of oriC and six, diverged by more than one nucleotide from a perfect palindromic sequence, dispersed along the chromosome. Here, we constructed and analyzed P. aeruginosa mutants deprived of each single parS sequence and their different combinations. The analysis included evaluation of a set of phenotypic features, chromosome segregation, and ParB localization in the cells. It was found that ParB binds specifically to all ten parS sites, although with different affinities. The P. aeruginosa parS mutant with all ten parS sites modified (parSnull) is viable however it demonstrates the phenotype characteristic for parAnull or parBnull mutants: slightly slower growth rate, high frequency of anucleate cells, and defects in motility. The genomic position and sequence of parS determine its role in P. aeruginosa biology. It transpired that any one of the four parS sites proximal to oriC (parS1 to parS4), which are bound by ParB with the highest affinity, is necessary and sufficient for the parABS role in chromosome partitioning. When all these four sites are mutated simultaneously, the strain shows the parSnull phenotype, which indicates that none of the remaining six parS sites can substitute for these four oriC-proximal sites in this function. A single ectopic parS2 (inserted opposite oriC in the parSnull mutant) facilitates ParB organization into regularly spaced condensed foci and reverses some of the mutant phenotypes but is not sufficient for accurate chromosome segregation.
| null |
1506_biomrc
|
Title: Sequential screening to predict XXXX after gynecologic surgery in Nara, Japan.
OBJECTIVE: To evaluate a sequential screening method's efficacy in predicting Patients, patients151 (PTE) after gynecologic surgery. METHODS: A prospective study employing a two-stage screening process was conducted among consecutive asymptomatic adults who underwent outpatient evaluation for gynecologic surgery at Nara Medical University Hospital, Japan, between April 1, 2004, and December 31, 2013. Patients, patients with a preoperative plasma D-dimer level greater than or equal to 1.0 g/mL underwent compression ultrasonography of the lower extremities. The primary outcome measure was postoperative detection of symptomatic PTE. RESULTS: Overall, 1729 Patients, patients were included. The mean D-dimer level was 1.7 3.3 g/mL. Compression ultrasonography was conducted among 470 (27.1%) Patients, patients with positive D-dimer test results; symptomatic Patients, patients150 ( Patients, patients150 ) was preoperatively detected among 94 (20.0%) of them. Patients, patients with Patients, patients150 (n=94) had higher D-dimer levels than Patients, patients (n=1635) without (7.8 12.8 g/mL vs 1.1 1.8 g/mL; P<0.001). Despite anticoagulant therapy, symptomatic PTE was detected postoperatively among two of these 94 Patients, patients . Symptomatic PTE was also detected among four of 376 Patients, patients with positive D-dimer test results but no evidence of Patients, patients150 by ultrasonography. No clinical onset of postoperative PTE was observed among 1259 Patients, patients with D-dimer levels below the cut-off value. CONCLUSION: The PROVEN screening strategy (Preoperative surveillance using a sequential strategy) was ineffective at predicting postoperative symptomatic PTE.
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3555_biomrc
|
Title: A phase I dose escalation study of biweekly @entity136 and XXXX in completely resected stage IB-IIIA @entity1149 .
OBJECTIVE: We conducted a phase I dose escalation study to determine the maximum tolerated dose, recommended dose, and safety profile of a biweekly Patients, patients, patient, PATIENTS36 and Carboplatin, carboplatin combination regimen in the treatment of Patients, patients, patient, PATIENTS with completely resected Patients, patients, patient, PATIENTS149 ( Patients, patients, patient, PATIENTS149 ). Patients, patients, patient, PATIENTS AND METHODS: Patients, patients, patient, PATIENTS with completely resected pathologically documented stage IB, II, or IIIA Patients, patients, patient, PATIENTS149 , performance status (ECOG) 0-1, with adequate bone marrow, Patients, patients, patient, PATIENTS77 , were treated with Patients, patients, patient, PATIENTS36 and Carboplatin, carboplatin . The starting dose was Patients, patients, patient, PATIENTS36 800 mg/m2 on days 1 and 15 and Carboplatin, carboplatin area under the time-concentration curve (AUC) 4 mg/mL/min on day 1. Patients, patients, patient, PATIENTS36 was increased to 1000 mg/m2 (level 3). Carboplatin, carboplatin was increased to AUC 5 (level 2, 3). The regimen was performed every 4 weeks. The dose-limiting Patients, patients, patient, PATIENTS37 of the regimen was assessed during the first chemotherapy cycle. RESULTS: Nine Patients, patients, patient, PATIENTS were enrolled in this study. All Patients, patients, patient, PATIENTS were assessed for safety. Grade 3 Patients, patients, patient, PATIENTS431 occurred in 1 Patients, patients, patient, PATIENTS (11%) and grade 3/4 Patients, patients, patient, PATIENTS398 occurred in 3 Patients, patients, patient, PATIENTS (33%). No other grade 3/4 Patients, patients, patient, PATIENTS37 was observed. No dose-limiting Patients, patients, patient, PATIENTS37 was experienced at dose levels 1, 2, and 3 of this schedule. CONCLUSION: Maximum tolerated dose was not reached in this study. Considering treatment continuation, the recommended dose for a phase II study is Patients, patients, patient, PATIENTS36 1000 mg/m2 on days 1 and 15 and Carboplatin, carboplatin AUC 5 on day 1, every 4 weeks. Biweekly administration of Patients, patients, patient, PATIENTS36 and Carboplatin, carboplatin is a feasible and well-tolerated regimen for the treatment of Patients, patients, patient, PATIENTS with completely resected Patients, patients, patient, PATIENTS149 as adjuvant chemotherapy.
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1510_biomrc
|
Title: Fine tuning of nanopipettes using XXXX for single molecule sensing.
UNASSIGNED: Nanopipettes are an attractive single-molecule tool for identification and characterisation of nucleic acids and proteins in solutions. They enable label-free analysis and reveal individual molecular properties, which are generally masked by ensemble averaging. Having control over the pore dimensions is vital to ensure that the dimensions of the molecules being probed match those of the pore for optimization of the signal to noise. Although nanopipettes are simple and easy to fabricate, challenges exist, especially when compared to more conventional solid-state analogues. For example, a sub-20 nm pore diameter can be difficult to fabricate and the batch-to-batch reproducibility is often poor. To improve on this limitation, atomic layer deposition, ALD ( atomic layer deposition, ALD ) is used to deposit ultrathin layers of alumina ( Al2O3 ) on the surface of the quartz nanopipettes enabling sub-nm tuning of the pore dimensions. Here, Al2O3 with a thickness of 8, 14 and 17 nm was deposited onto pipettes with a starting pore diameter of 75 5 nm whilst a second batch had 5 and 8 nm Al2O3 deposited with a starting pore diameter of 25 3 nm respectively. This highly conformal process coats both the inner and outer surfaces of pipettes and resulted in the fabrication of pore diameters as low as 7.5 nm. We show that Al2O3 modified pores do not interfere with the sensing ability of the nanopipettes and can be used for high signal-to-noise DNA detection. atomic layer deposition, ALD provides a quick and efficient (batch processing) for fine-tuning nanopipettes for a broad range of applications including the detection of small biomolecules like RNA, aptamers and DNA-protein interactions at the single molecule level.
| null |
3563_biomrc
|
Title: Efficacy of motor cortex stimulation for intractable central XXXX : comparison of stimulation parameters between post- @entity64 @entity158 and other @entity158 .
Motor cortex stimulation, MCS ( Motor cortex stimulation, MCS ) has now become the preferred option for neurosurgical management of intractable central neuropathic pain such as post- stroke patients, patient58 and trigeminal neuropathic pain . However, the efficacy of Motor cortex stimulation, MCS for other central neuropathic pain such as patients, patient58 resulting from spinal cord or brainstem lesions, brainstem lesion is unclear. We retrospectively reviewed 11 consecutive patients, patient with intractable central neuropathic pain who underwent Motor cortex stimulation, MCS in our institution. Eight patients, patient had patients, patient58 caused by thalamic hemorrhage (n = 5) or patients, patient188 (n = 3) (thalamic group). Two patients, patient had postoperative neuropathic pain caused by spinal cord lesions , and one patients, patient had patients, patient58 caused by a brainstem lesions, brainstem lesion associated with patients, patient5 (brainstem-spinal group). Visual analog scale and stimulation parameters were evaluated at 1 and 6 months postoperatively. Motor cortex stimulation, MCS was effective for six of eight patients, patient in the thalamic group, and all three patients, patient in the brainstem-spinal group. These efficacies continued for 6 months after surgery without significant change in the stimulation parameters compared with the parameters at 1 month in both groups. The mean amplitude at 1 month and frequency at 6 months after surgery were significantly higher in the brainstem-spinal group than the thalamic group, although the patients, patient number was small. Motor cortex stimulation, MCS is effective for other central neuropathic pain , but higher intensity stimulation parameters may be necessary to gain adequate patients, patient58 reduction.
| null |
1522_biomrc
|
Title: Increases in @entity645 incidence among @entity1 who have sex with @entity1 undergoing repeat diagnostic XXXX testing in Ontario, Canada.
OBJECTIVE: To estimate HIV incidence density for different exposure categories among Persons, person, people, men undergoing repeat testing in Ontario, Canada. METHODS: Persons, person, people, men using voluntary, diagnostic HIV testing at least twice were identified by computerized and manual record linkage. In the 1992-2000 period, 980 seroconverters and 340 994 repeat negative testers contributed 936 145 Persons, person, people, men years (PY) of observation. ID, Incidence density ( ID, Incidence density ) was calculated according to Kitayaporn et al. Poisson regression was used to evaluate differences in incidence. RESULTS: Among Persons, person, people, men who have sex with Persons, person, people, men (MSM), ID, Incidence density declined between 1992-1996, from 1.23 per 100 PY in 1992 to 0.79 per 100 PY in 1996 [relative risk (RR), 0.86 per year; 95% confidence interval (CI), 0.77-0.96]. Subsequently, ID, Incidence density increased to 1.39 per 100 PY in 1999 (RR, 1.18 per year; 95% CI, 1.05-1.34). In 2000, ID, Incidence density was 1.16 per 100 PY but this decrease was not statistically significantly different from 1999. MSM in their twenties had the highest ID, Incidence density in 1992-1996, but in 1996-2000 MSM in their thirties had the highest risk of infection . Among injecting drug users (IDU), ID, Incidence density decreased from 0.64 per 100 PY in 1992 to 0.14 per 100 PY in 2000 (RR, 0.87 per year; 95% CI, 0.80-0.94). Among heterosexuals, annual incidence remained constant at about 0.03 per 100 PY in 1992-2000. CONCLUSIONS: Increases in ID, Incidence density were identified among MSM from 1996 to 1999. These findings are consistent with other research. Continued vigilance and improved surveillance are needed to better understand and control the epidemic.
| null |
1525_biomrc
|
Title: Chemical quality of maternal drinking water and XXXX .
We undertook a case-control study to investigate the association between chemicals in maternal drinking water consumed during pregnancy and congenital heart disease in the offspring. Two hundred and seventy affected children and 665 healthy children were enrolled in the study. Information on contaminant levels in maternal drinking water was available from records of routine water analysis of samples taken from public taps in the communities where the mothers resided during pregnancy. Mothers provided information during a telephone interview on their health, pregnancy management, and demographic characteristics. Nine inorganic metals were analysed for detection of an association with congenital heart disease . The chemical exposures of particular interest were mercury4 , lead, mercury and selenium . None of the chemicals was associated materially with an increase in the frequency of congenital heart disease overall. mercury4 exposure at any detectable level was associated with a threefold increase in occurrence of children498 (prevalence odds ratio = 3.4, 95% confidence interval = 1.3-8.9). Detectable traces of selenium in drinking water were associated with a lower frequency of any congenital heart disease than was observed among children exposed to drinking water not containing detectable levels of selenium (prevalence odds ratio = 0.62, 95% confidence limits = 0.40-0.97). A dose-response effect was observed over four levels of selenium exposure. Non-differential errors in the measurement and classification of exposure to contaminants routinely monitored in drinking water could account for lack of positive findings. In addition, most of the contaminant levels were below the maximum levels set by the Environmental Protection Agency, so that lack of evidence of effect may have been due to the low exposure levels in this population.
| null |
1526_biomrc
|
Title: Retrospective comparison between a regular and a split-dose protocol of @entity1487 , @entity900 , and @entity2664 for the treatment of far XXXX .
BACKGROUND: In Child, Patients, patients with Child, Patients, patients57 ( Child, Patients, patients57 ), combination chemotherapy using Child, Patients, patients487 , cisplatin , and mitoxantrone (FMP) could achieve a response rate > 20%, but the beneficial effect was compromised by formidable adverse events. Chemotherapy given in a split-dose manner was associated with reduced Child, Patients, patients37 . In this retrospective study, we compared the efficacies and side effects between a regular and a split-dose FMP protocol approved in our medical center. METHODS: From 2005 to 2008, the clinical data of 84 Child, Patients, patients with far advanced Child, Patients, patients57 , who had either main portal vein thrombosis and/or extrahepatic metastasis , were reviewed. Of them, 65 were treated by either regular (n = 27) or split-dose (n = 38) FMP and had completed at least one therapeutic course. The remaining 19 Child, Patients, patients were untreated. Clinical parameters, therapeutic responses, survivals and adverse events were compared. RESULTS: The median overall survival was 6.0, 5.2, and 1.5 months, respectively, in Child, Patients, patients receiving regular FMP, split-dose FMP, and no treatment (regular versus split-dose group, P = 0.447; regular or split-dose versus untreated group; P < 0.0001). Child, Patients, patients receiving split-dose treatment had a significantly lower risk of grade 3/4 Child, Patients, patients398 (51.9 versus 10.5%, P = 0.0005). When the two treated groups were combined, the median overall survival was 10.6 and 3.8 months respectively for Child, Patients, patients achieving disease control and progressive disease (P < 0.001). Cox proportion hazard model identified Child, Patients, patients -Pugh stage B (hazard ratio [HR], 2.216; P = 0.006), presence of extrahepatic metastasis (HR, 0.574; P = 0.048), and achievement of disease control (HR, 0.228; P < 0.001) as independent factors associated with overall survival. Logistic regression analysis revealed that Child, Patients, patients0745 virus antibody (odds ratio [OR], 9.219; P = 0.002) tumor, cancer size (OR, 0.816; P = 0.036), and previous anti- tumor, cancer therapy (OR, 0.195; P = 0.017) were significantly associated with successful disease control. CONCLUSIONS: Comparable overall survival was observed between Child, Patients, patients receiving regular and split-dose FMP therapies. Child, Patients, patients receiving split-dose therapy had a significantly lower risk of grade 3/4 Child, Patients, patients398 . Child, Patients, patients0745 virus antibody, smaller tumor, cancer size, and absence of previous anti- tumor, cancer therapy were independent predictors for successful disease control.
| null |
3578_biomrc
|
Title: The effect of intraoperative @entity6948 on postoperative catheter-related bladder discomfort in XXXX undergoing transurethral @entity150 resection: A prospective, double-blind, randomised study.
BACKGROUND: Patients, patients, PATIENTS494 ( Patients, patients, PATIENTS494 ) due to an indwelling urinary catheter causes postoperative distress. dexmedetomidine, Dexmedetomidine is used as an anaesthetic adjuvant during general anaesthesia and has an antimuscarinic effect, which may be beneficial for the prevention and treatment of Patients, patients, PATIENTS494 . OBJECTIVE: To determine the effect of intraoperative dexmedetomidine, Dexmedetomidine administration on the incidence of Patients, patients, PATIENTS494 . DESIGN: A prospective double-blind, placebo-controlled, randomised study. SETTING: A tertiary care teaching hospital. Patients, patients, PATIENTS : One-hundred and nine Patients, patients, PATIENTS undergoing transurethral Patients, patients, PATIENTS50 excision (TURB). INTERVENTIONS: Patients, patients, PATIENTS were randomly allocated to two groups: control group (n=55) received placebo and dexmedetomidine, Dexmedetomidine group (n=54) received intraoperative dexmedetomidine, Dexmedetomidine (1 gkg loading dose followed by 0.5 gkghcontinuous infusion). MAIN OUTCOME MEASURES: The incidence and severity (mild, moderate, severe) of Patients, patients, PATIENTS494 assessed at 0, 1, 6 and 24h postoperatively. RESULTS: The incidence of Patients, patients, PATIENTS494 was significantly higher in the control group at 0 (78 vs. 50%; P=0.004), 1 (86 vs. 57%; P=0.002) and 6h (82 vs. 63%; P=0.047) postoperatively. The incidence of moderate to severe Patients, patients, PATIENTS494 was higher in the control group at 0 (38 vs. 11%; P=0.002) and 1h (29 vs. 7%; P=0.006) postoperatively. The number of Patients, patients, PATIENTS having Patients, patients, PATIENTS494 treated with Patients, patients, PATIENTS340 was higher in the control group (24 vs. 12; P=0.006). The mean end-tidal desflurane concentration during the surgery was higher in the control group (4.5 vs. 3.9%; P=0.04). The postoperative pain score (numerical rating scale: 0 to 10) was higher in the control group at 0 (4.6 vs. 2.7; P=0.002), and 1h (3.8 vs. 2.7; P=0.041). The number of Patients, patients, PATIENTS treated with opioids was higher in the control group (21 vs. 8; P=0.011). CONCLUSION: Intraoperative dexmedetomidine, Dexmedetomidine administration decreased the incidence and severity of early postoperative Patients, patients, PATIENTS494 as well as intraoperative Patients, patients, PATIENTS0233 and postoperative opioid requirements in Patients, patients, PATIENTS undergoing TURB. TRIAL REGISTRATION IDENTIFIER: NCT01991223 (www.clinicaltrials.gov).
| null |
1530_biomrc
|
Title: Corneal characteristics in myopic XXXX .
OBJECTIVES: To evaluate the characteristics of the cornea in myopic patients . MATERIAL AND METHOD: A retrospective study of 420 eyes in 210 patients who visited the Excimer Laser Surgery Clinic at Siriraj Hospital, Mahidol University from January 1999 to June 2002 was conducted. All cases that were eligible for myopic refractive surgery were included in the present study. A preoperative ocular examination was done in a fashionable method, including manifest refraction and Orbscan Topography to evaluate corneal thickness, corneal curvature and corneal diameter Statistical analysis was performed to identify the characteristics of the cornea in the patients . RESULTS: The mean age of the patients was 31.66 +/- 7.77 years. (ranging from 16-51). The mean manifest refraction (spherical equivalent) was -4.9 +/- 2.29 diopters (ranging from -0.50 to -13.75). The mean corneal diameter (white to white) was 11.60 mm +/- 0.37 mm (ranging from 10.8-12.9). The thinnest point of the cornea varied from 409 to 597 microns (mean = 522.55) and located mostly in the infero-temporal quadrant of the eye (23.69% in the right and 32.05% in the left). The mean curvature of the corneal was 44.6 +/- 13.8 diopters (ranging from 39.05-47.65). The mean corneal astigmatism was 1.34 +/- 0.71 diopters and 93.96% were with the rule astigmatism . Positive angle kappa was found in 408 eyes (97%) but all cases were within a 0.5 mm horizontal and 0.3 mm vertical radius from the pupillary axis . CONCLUSION: Characteristics of the cornea in Thai myopic patients were demonstrated in the present study. Most of the cornea showed with the rule astigmatism with positive angle kappa and the thinnest point, located at the infero-temporal quadrant.
| null |
1533_biomrc
|
Title: Alterations in cell cycle genes in early stage XXXX identified by expression profiling.
In normal lung epithelial cells, cellular division is an ordered, tightly regulated process involving multiple checkpoints that assess extracellular growth signals, cell size, and DNA integrity. In contrast, neoplastic lung cells develop the ability to bypass several of these checkpoints, particularly at the G1/S and G2/M boundaries. We used genomic profiling to compare gene expression levels in early stage lung cancer220 and non-neoplastic pulmonary tissue in order to comprehensively identify alterations in the process of cell cycling. RNA extracted from node negative, poorly differentiated lung cancer220 (15 patients ) and non-neoplastic pulmonary tissue (5 patients ) was hybridized to oligonu-cleotide microarray filters containing 44,363 genes. Ontological classification was used to extract genes involved with cell cycle progression. Further analysis discovered a subset of differentially expressed genes for further study. Of the 624 cell cycle genes on the microarray filters, 40 genes were predicted to be differentially expressed in lung adeno- patients89 . Alterations in several genes (i.e., cyclin B1 , cyclin D1 , p21 , MDM2 ) are consistent with published data in the literature. We also identified 19 novel genes that have neither been described in patients149 (i.e., patients0610 , cullin 4A , ZAC , p57 , DP-1 , GADD45, PISSLRE , cdc20 ) nor in any other tumors (i.e., cyclin F , lung cancer3901 , patients3225 ). These results identified several potential cell cycle genes altered in lung cancer .
| null |
3583_biomrc
|
Title: Phenotypic variations and dynamic topography of transformed cells in an experimental model of XXXX -induced @entity703 in male @entity329 .
This work explores the phenotypic changes affecting transformed cells in an experimental model of diethylstilbestrol ( DES )-induced renal tumours, kidney tumours in male Syrian hamster . This estrogen -induced neoplasms, neoplasm, tumour presents an important cytological pleomorphism and its origin remains largely controversial. In order to characterize phenotypic variations during neoplasms, neoplasm, tumour progression, the occurrence of seven lineage markers was analysed by a morphometric approach in kidney sections of DES -exposed hamsters (6-11 months). S100 protein, neuron-specific enolase (NSE) and vimentin are expressed by a large percentage of malignant cells during neoplasms, neoplasm, tumour development. Glial fibrillary acidic protein (GFAP), protein gene product 9.5 (PGP 9.5) and desmin are mostly evidenced in advanced neoplasms, neoplasm, tumour whereas Leu 7 always presents a focal expression. As evidenced by double-label immunofluorescence, the coexpression of three important neuroectodermal lineage markers (S100, NSE and PGP 9.5) in earliest neoplasms, neoplasm, tumour buds points to a peripheral nerve sheath origin for this neoplasms, neoplasm, tumour thus confirming previously published data. For each marker, the fluctuations of expression levels during neoplasms, neoplasm, tumour progression as well as the spatial heterogeneity of distribution suggest variable phenotypic differentiation of transformed cell populations. This observation is largely corroborated by double-label immunofluorescence showing coexpression modification of several markers during neoplasms, neoplasm, tumour progression. This points to a complex dynamic and spatial self-organization of different phenotypes within neoplasms, neoplasm, tumour . Altogether, these results support the concept that DES -induced renal tumours, kidney tumours are not made of unstructured cell populations but represent adaptive complex dynamic biosystems.
| null |
1535_biomrc
|
Title: s-IgA response in females following a single bout of moderate intensity exercise in XXXX and thermoneutral environments.
Previous research has linked exercise under unfavourable environmental conditions to decreased concentration and/or secretion rate of secretory immunoglobulin A (s-IgA). The purpose of this study was to investigate the influence of moderate exercise in a thermoneutral (TN) and a cold ( COLD ) environment on the concentration of s-IgA and the secretion rate of s-IgA in moderately active females. Sixteen females (23.4 +/- 6.8 yrs, 61.1 +/- 5.9 kg, 1.64 +/- 0.07 m) served as subjects for this study. On separate occasions, one week apart, all subjects ran or walked for 30 minutes at 71 % of individual heart rate reserve on an outdoor (1 degrees C) or an indoor track (24 degrees C). Unstimulated saliva samples were collected for 4-min immediately pre (PR), post (POST), and 30-min post (30POST) exercise. Profile of Mood State (POMS) questionnaires were completed pre and post exercise intervention and Global Mood State (GMS) was calculated. The absolute concentration of s-IgA, and total non-specific protein were measured and the secretion rate of s-IgA and s-IgA:Protein were calculated. Analysis of Variance (ANOVA) revealed that flow rate increased in COLD and decreased in TN such that it was significantly lower (POST) and (30POST) in the TN environment when compared to COLD . There was a significant increase in the secretion rate of s-IgA at 30POST for the COLD trial and a significant decrease at 30POST for the TN trial. No significant differences were found for the concentration of s-IgA, the s-IgA:Protein:ratio, or GMS. Stepwise regression revealed that with all predictors in the model, only the concentration of s-IgA accounted for a significant proportion of the variance in cold temperature, while in the TN environment, both the concentration of s-IgA and the flow rate accounted for significant proportions of the variance. These findings suggest that moderate exercise in cold temperatures does not decrease secretion rate of s-IgA, while exercise in TN temperatures does.
| null |
3586_biomrc
|
Title: XXXX Plays an Important Role in @entity63 -Induced Angiogenesis of Limbs.
LOX-1 and 3, LOX-1 , lectin-like oxidized low-density lipoprotein (LDL) receptor-1, is a single transmembrane receptor mainly expressed on endothelial cells. LOX-1 and 3, LOX-1 mediates the uptake of oxidized LDL, an early step in atherosclerosis ; however, little is known about whether LOX-1 and 3, LOX-1 is involved in angiogenesis during tissue ischemia, ischemic . Therefore, we examined the role of LOX-1 and 3, LOX-1 in ischemia, ischemic -induced angiogenesis in the hindlimbs of LOX-1 and 3, LOX-1 knockout (KO) mice . Angiogenesis was evaluated in a surgically induced hindlimb ischemia, ischemic model using laser Doppler blood flowmetry (LDBF) and histological CD, capillary density ( CD, capillary density ) and AD, arteriole density ( AD, arteriole density ). After right hindlimb ischemia, ischemic , the ischemia, ischemic /nonischemic hindlimb blood flow ratio was persistently lower in LOX -1 KO mice than in WT, wild-type ( WT, wild-type ) mice . CD, capillary density and AD, arteriole density were significantly smaller in LOX -1 KO mice than in WT, wild-type mice on postoperative day 14. Immunohistochemical analysis revealed that the number of macrophages infiltrating ischemia, ischemic tissues was significantly smaller in LOX -1 KO mice than in WT, wild-type mice . The number of infiltrated macrophages expressing VEGF was also significantly smaller in LOX -1 KO mice than in WT, wild-type mice . Western blot analysis and ROS production assay revealed that LOX - KO mice show significant decrease in Nox2 expression, ROS production and mice65 expression, the phosphorylation of mice95 and NF-kB p65 subunit as well as expression of redox-sensitive VCAM-1, vascular cell adhesion molecule-1 ( VCAM-1, vascular cell adhesion molecule-1 ) and LOX-1 and 3, LOX-1 itself in ischemia, ischemic muscles, which is supposed to be required for macrophage infiltration expressing angiogenic factor VEGF . Reduction of VEGF expression successively suppressed the phosphorylation of Akt and eNOS, which accelerated angiogenesis, in the ischemia, ischemic leg of LOX -1 KO mice . Our findings indicate that LOX-1 and 3, LOX-1 plays an important role in ischemia, ischemic -induced angiogenesis by 1) Nox2 -ROS-NF-kB activation, 2) upregulated expression of adhesion molecules: VCAM-1, vascular cell adhesion molecule-1 and LOX-1 and 3, LOX-1 ) promoting macrophage infiltration, which expresses angiogenic factor VEGF .
| null |
1539_biomrc
|
Title: Huge caudate lobe of the liver due to XXXX .
hepatic venous outflow obstruction, Budd-Chiari syndrome is characterized by hepatic venous outflow obstruction, Budd-Chiari syndrome . We describe a woman, patient with a huge caudate lobe of the liver due to hepatic venous outflow obstruction, Budd-Chiari syndrome . A 49-year-old woman, patient was referred to Nippon Medical School Hospital to receive treatment for enlarged gastric varices, solitary gastric varices . She had been followed up for idiopathic portal hypertension with deformity of the liver for 7 years and had undergone surgery for woman, patient89 5 years earlier. Upper gastrointestinal endoscopy revealed gastric varices, solitary gastric varices without esophageal varices. Abdominal computed tomography revealed obstructions and scars of the right, middle, and left hepatic veins. The caudate lobe was enlarged, and the portal vein was displaced ventrally, without thrombus . The drainage vein of the caudate lobe, the short hepatic vein on the left side of the inferior vena cava, was dilated. The portal vein and drainage vein of segment 6 were visualized and showed no woman, patient94 . Venography revealed no obstruction of the inferior vena cava. hepatic venous outflow obstruction, Budd-Chiari syndrome with gastric varices, solitary gastric varices was diagnosed on the basis of these findings. We performed balloon-occluded retrograde transvenous obliteration and partial splenic embolization to treat the gastric varices, solitary gastric varices . The posttreatment course was uneventful, and the woman, patient was discharged 8 days after embolization. The gastric varices, solitary gastric varices shrank.
| null |
3589_biomrc
|
Title: Diffusion weighted magnetic resonance imaging for XXXX : practical and popular.
AIM: To evaluate the feasibility and impact of diffusion weighted magnetic resonance imaging (DW MRI) as the first line neuroimaging of stroke, acute stroke at a district general hospital. METHODS: Prospective audit of all in- patients admitted with clinically suspected stroke, acute stroke and referred for imaging over a consecutive 17 week period. The data collected included scan type, time from cerebral event to imaging request, and time from formal radiological request to neuroimaging. Clinicians' (general physicians, neurologists, and radiologists) perceptions were assessed by a questionnaire. RESULTS: 148 patients had neuroimaging for clinically suspected stroke, acute stroke during this period. Eighty one per cent of patients (120 of 148) had DW MRI as first line. Ninety two per cent of these patients had DW MRI within 24 hours of the formal radiological request. Twenty eight patients did not undergo DW MRI because lack of MRI safety, clinical state, unavailability because of maintenance service or lack of trained staff. Clinicians found the introduction of the DW MRI based service a significant improvement on computed tomography, especially for equivocal cases. CONCLUSION: DW based MRI service is both feasible and sustainable in the setting of a district general hospital and most clinicians feel that this is a significant improvement to stroke, acute stroke services.
| null |
3591_biomrc
|
Title: Surgical management of a XXXX : a case report.
BACKGROUND: A primary chondromyxoid fibroma, CMF ( chondromyxoid fibroma, CMF ) arising from sternum is quite uncommon tumor, Removal of giant sternal tumors in thoracic surgery. tumor, Removal of giant sternal tumors requires extensive resection of the anterior chest wall, and results in deformity and paradoxical movement . CASE PRESENTATION: A 40-year-old female presented a progressively enlarging mass of her anterior chest wall. Computed tomography revealed an osteolytic lesion with discrete calcification in the bone marrow of the sternum. The tumor, Removal of giant sternal tumors extended across the destroyed cortex to the parietal and visceral soft aspects, involving some of the costal cartilage and most of the sternal body. Partial sternal resection was performed successfully and an individual-specific stainless steel plate was used to reconstruct the anterior chest wall. The early result was good, however, nine months after the first surgery, fractures of plate were found at bilateral plate-clavicular junction. The plate had to be removed, and a titanium mesh was used to reconstruction of the chest wall. The patient has been of disease free for more than 18 month after the second surgery. CONCLUSIONS: Our experience indicated that the individual-specific plate may not be suitable for reconstructing both the anterior chest wall as well as the sternoclavicular joint after subtotal sternum resection.
| null |
3596_biomrc
|
Title: Pathology of difficult XXXX .
Severe asthma is poorly understood clinically, physiologically and pathologically. Although milder forms of asthma are generally easily treated, more severe forms remain refractory to the best current medical care. Both genetic and environmental elements are likely to play an important role in the development of severe disease . Physiologically, these patients, patient often have air-trapping, airway collapsibility and loss of elastic recoil. The pathology demonstrates a heterogeneity of findings and is based mainly on adult studies. Some patients, patient demonstrate continued eosinophilic inflammation despite the use of high doses of corticosteroids . At least one-third appear to have pathological changes inconsistent with classically described asthmatic pathology. Persistent inflammation can lead to structural changes involving the airways and perhaps the parenchyma as well. Finally, inflammation and structural changes appear to exist well into the lung periphery, out of reach of many inhaled medications. The importance of each of these factors in the development of severe disease probably varies from patients, patient to patients, patient . Understanding the commonalities of the pathology of severe asthma should, however, lead to the development of improved therapies.
| null |
1555_biomrc
|
Title: Strategy of treatment of XXXX during pregnancy. The Drugs and Pregnancy Study Group.
OBJECTIVE: To present a procedure for establishing treatment guidelines during pregnancy and, as an application of the method, the recommendations for treatment of participants, women, PARTICIPANTS11 . DESIGN: A preliminary survey was carried out among participants, women, PARTICIPANTS of the study group to determine drug choices for treatment of participants, women, PARTICIPANTS11 during pregnancy. Literature data on the selected drugs were analyzed. Recommendations for treatment were proposed, taking into account for most efficient agents and the least toxic for the fetus. This draft was submitted to all participants, women, PARTICIPANTS of the study group; criticism and suggestions were gathered. A new draft was evolved and repeatedly submitted to participants, women, PARTICIPANTS until consensus was reached. SETTING: Study group set up in southwestern France, with the help of national experts. participants, women, PARTICIPANTS : Forty-three general practitioners, six gynecologists and/or obstetricians, five pharmacologists, two dermatologists, and an embryologist. RESULTS: The main recommendations for treatment of participants, women, PARTICIPANTS11 during pregnancy are to begin with topical treatment; emollient bath additives, moisturizing cream, talc. If insufficient, a systemic treatment should be added. Antihistamines are prescribed first: hydroxyzine or dexchlorpheniramine is used during the first 2 months. From the third month, the same agents can be used, as can mequitazine . The duration of treatment can be up to 10 days. The group's second choice was participants, women, PARTICIPANTS245 ( oxazepam ) as second-line treatment. CONCLUSIONS: Strategies for treatment of diseases during pregnancy are not always well defined. For many common diseases, very few data are available concerning drug use in pregnant participants, women, PARTICIPANTS . The study group, based on consensus among participants, women, PARTICIPANTS , proposed treatment guidelines.
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3608_biomrc
|
Title: XXXX , @entity458 : a pilot study.
INTRODUCTION: In polycystic ovarian syndrome, PCOS ( polycystic ovarian syndrome, PCOS ) women, woman, patients , the changes in body appearance (mainly obesity and hirsutism ) may influence the feminine identity of the women, woman, patients with consequent Depression, depression and sexual disturbances, sexual self-worth and sexual satisfaction . AIM: To evaluate if lean polycystic ovarian syndrome, PCOS women, woman, patients present an increased incidence of Depression, depression and sexual dysfunction in comparison with controls and if clitoral volume and vascularization are influenced by circulating androgens levels. METHODS: 25 lean polycystic ovarian syndrome, PCOS women, woman, patients (Group I) and 18 healthy nonhirsute volunteers (Group II) were submitted, on day 3-5 of the cycle, to ultrasonographic and Doppler analyses, and to hormonal and biochemical evaluations. MAIN OUTCOME MEASURES: Utero-ovarian and clitoral ultrasonographic analysis, and color Doppler evaluation of the uterine, stromal ovarian, and dorsal clitoral arteries. Hormonal and nitrites / nitrates plasma concentrations were analyzed. Each women, woman, patients filled in the 2-factor Italian McCoy female questionnaire (MFSQ) and the Beck's Depression, depression Inventory questionnaire (BDI). RESULTS: Androgens resulted, as expected, more elevated in polycystic ovarian syndrome, PCOS women, woman, patients than in controls. However, the ultrasonographic (US) assessment of the clitoral body volume evidenced no significant differences between polycystic ovarian syndrome, PCOS (0.72+/-0.41 mL) and control (0.62+/-0.20 mL) women, woman, patients . The resistances registered at the level of the dorsal clitoral artery did not show any difference between Group I (PI=1.55+/-0.40) and Group II (PI=1.79+/-0.38). The 2-factor Italian MFSQ and the BDI did not show any difference between polycystic ovarian syndrome, PCOS women, woman, patients and controls. CONCLUSIONS: In polycystic ovarian syndrome, PCOS women, woman, patients , probably, the moderate hirsutism and hyperandrogenism do not induce the sense of loss of feminine identity and have no impact on sexual disturbances, sexual self-worth and sexual satisfaction .
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1561_biomrc
|
Title: Neurohumoral interactions in conscious dehydrated XXXX .
The purpose of this study was to examine the role of angiotensin II (ANG II), arginine vasopressin ( AVP ), and neurogenic mechanisms in maintaining arterial pressure (MAP) in conscious water-deprived rabbits . Water deprivation produced marked increases in plasma renin activity and the concentration of AVP; however, plasma catecholamine concentrations were unchanged. Arterial baroreflex control of renal sympathetic nerve activity (RSNA) and heart rate (HR) was similar in water-replete and dehydrated animals. Administration of an ANG II receptor antagonist (ANG IIX) to water-deprived animals produced a small decrease in MAP but no significant changes in HR or mesenteric and hindquarters vascular resistances. Similarly, there was no significant effect on MAP, HR, or regional hemodynamics when dehydrated animals received an AVP-V1 antagonist (AVPX). RSNA increased by maximums of 61 and 43% in response to ANG IIX and AVPX, respectively. Combined administration of ANG IIX and AVPX produced significant decreases in MAP (-9 mmHg) and hindquarters resistance (-24%) and 117% and 23 beat/min increases in RSNA and HR, respectively. The effect on mesenteric resistance was variable; however, the response was generally a decrease. We conclude that ANG II and AVP pressor mechanisms are activated to sustain MAP in the dehydrated state. In contrast, the basal level and baroreflex control of sympathetic nervous system activity are unchanged from the water-replete state. However, activation of sympathetic nerve activity may become important in maintaining peripheral resistance when ANG II and AVP receptors are blocked in water-deprived animals.
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3618_biomrc
|
Title: [Severe XXXX from heparin: value of the use of low molecular weight heparin. Apropos of 6 cases].
Severe heparin-induced thrombocytopaenia associated with patients031 is a well known complication, although the exact pathogenic mechanism remains unclear. It sets the problem of whether to continue heparin therapy because standard heparin must be withdrawn. Heparin is a mucopolysaccharide composed of fractions of different molecular weights. The fractions with high molecular weights have been held responsible for these severe thrombocytopenias and so, the use of low molecular weight heparin has been suggested. The authors used subcutaneous low molecular weight heparin ( patients1270 Choay Institute) at empirical doses of 350 to 1 500 units/kg/24 hour in six cases of severe heparin-induced thrombocytopaenia. Platelet counts rapidly returned to normal (4 days on average) in 5 cases. Thrombocytopaenia persisted with low molecular weight heparin in 1 case. The study of patients423 was positive with low molecular weight heparin in this case and the platelet count returned to normal when the treatment was withdrawn. The authors conclude that, although low molecular weight heparin is useful in severe heparin-induced thrombocytopaenia, its efficacy remains modest. Not only may patients423 persist with low molecular weight heparin which rekindles the debate as to its pathogenic mechanism, but also low molecular weight heparin may have a slight antithrombin effect which limits its use in patients at high risk of patients031 , imposing treatment with fast acting vitamin K antagonists.
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3619_biomrc
|
Title: XXXX Abnormalities in Current Clinical Practice: Frequency, Causes, Severity and Management.
OBJECTIVE: We aimed to investigate the prevalence and etiology of potassium abnormalities ( patients371 and hyperkalemia ) and management approaches for hospitalized patients . SUBJECTS AND METHODS: Over a 4-month period, all hospitalized patients at Hacettepe University Medical Faculty Hospitals who underwent at least one measurement of serum potassium during hospitalization were included. Data on serum levels of electrolytes, demographic characteristics, cause(s) of hospitalization, medications, etiology of potassium abnormality and treatment approaches were obtained from the hospital records. RESULTS: Of the 9,045 hospitalized patients , 1,265 (14.0%) had a serum potassium abnormality; 604 (6.7%) patients had patients371 and 661 (7.30%) had hyperkalemia . In the hypokalemic patients , the most important reasons were gastrointestinal losses in 555 (91.8%) patients and renal losses in 252 (41.7%) patients . The most frequent treatment strategies were correcting the underlying cause and replacing the potassium deficit. Of the 604 hypokalemic patients , 319 (52.8%) were normokalemic at hospital discharge. The most common reason for hyperkalemia was treatment with renin -angiotensin- patients369 system blockers in 228 (34.4%) patients , followed by patients77 in 191 (28.8%). Two hundred and ninety-eight (45.0%) patients were followed without any specific treatment. Of the 661 hyperkalemic patients , 324 (49.0%) were normokalemic at hospital discharge. CONCLUSION: This study showed a high prevalence of potassium imbalance among hospitalized patients . Although most of the potassium abnormalities were mild/moderate, approximately half of the patients treated for patients371 or hyperkalemia were discharged from the hospital with ongoing dyskalemia. 2015 S. Karger AG, Basel.
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3623_biomrc
|
Title: Mutation analysis of the apoptotic "death-receptors" and the adaptors @entity45317 and XXXX / @entity23903 in @entity421 samples and @entity421 cell lines.
Apoptosis is a key mechanism of the organism that regulates embryogenesis and development, maintains homeostasis of the immune system and removes potentially hazardous cells. A dysregulation of apoptosis signaling may thus disturb the balance of cell survival and cell death, leading to the development of several diseases including tumor, cancer . In order to determine whether osteosarcomas, osteosarcoma, osteosarcoma tumor display an increased frequency of genetic alterations that affect apoptosis signaling, we analyzed the death domains of the death receptor genes Apo1, CD95 /Fas/ Apo1, CD95 , TNFR1 , TRAMP, Apo3, DR3, WSL-1 / TRAMP, Apo3, DR3, WSL-1 / TRAMP, Apo3, DR3, WSL-1 /LARD/ TRAMP, Apo3, DR3, WSL-1 , TRAIL-R2, DR5, TRICK2 / TRAIL-R2, DR5, TRICK2 / TRAIL-R2, DR5, TRICK2 /KILLER, DR6 and the complete coding sequences of the death receptor gene TRAIL-R1, DR4 / TRAIL-R1, DR4 and the genes of the adaptors TRADD and FADD, MORT-1 / FADD, MORT-1 . The investigation included 15 osteosarcomas, osteosarcoma, osteosarcoma tumor samples, 3 osteosarcomas, osteosarcoma, osteosarcoma tumor cell lines (SAOS-2, tumor, cancer187 and MG63) and peripheral blood from 20 donors as controls. We were able to identify 4 different sequence variations within the TRAIL-R1, DR4 gene located on exons 3, 4, 5 and 10 (death-domain). No alterations have been detected in the other genes or exons investigated. Except the sequence variant affecting exon 4, the alterations were homozygous in 15% of the tumor, cancer samples and cell lines, whereas the same alterations found in the control group were heterozygous or even not detectable. Three out of 4 alterations are located in the receptor's extracellular cysteine rich domain, which contains the ligand binding area and 1 on exon 10 coding for the death-domain. They may thus exert influence on ligand-receptor interactions and subsequent apoptosis induction. Our findings suggest that homozygous genetic alterations within the TRAIL-R1, DR4 gene may be implicated in the formation of osteosarcomas, osteosarcoma, osteosarcoma tumor .
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1577_biomrc
|
Title: XXXX Incidence and PSA Testing Patterns in Relation to USPSTF Screening Recommendations.
Importance: prostate cancer, Prostate cancer incidence in men 75 years and older substantially decreased following the 2008 US Preventive Services Task Force (USPSTF) recommendation against men486 (PSA)-based screening for this age group. It is unknown whether incidence has changed since the USPSTF recommendation against screening for all men in May 2012. Objective: To examine recent changes in stage-specific prostate cancer, Prostate cancer incidence and PSA screening rates following the 2008 and 2012 USPSTF recommendations. Design and Settings: Ecologic study of age-standardized prostate cancer, Prostate cancer incidence (newly diagnosed cases/100 000 men aged >= 50 years) by stage from 2005 through 2012 using data from 18 population-based Surveillance, Epidemiology, and End Results (SEER) registries and PSA screening rate in the past year among men 50 years and older without a history of prostate cancer, Prostate cancer who responded to the 2005 (n = 4580), 2008 (n = 3476), 2010 (n = 4157), and 2013 (n = 6172) National Health Interview Survey (NHIS). Exposures: The USPSTF recommendations to omit PSA-based screening for average-risk men . Main Outcomes and Measures: prostate cancer, Prostate cancer incidence and incidence ratios (IRs) comparing consecutive years from 2005 through 2012 by age (>= 50, 50-74, and >= 75 years) and SEER summary stage categorized as local/regional or distant and PSA screening rate and rate ratios (SRRs) comparing successive survey years by age. Results: prostate cancer, Prostate cancer incidence per 100 000 in men 50 years and older (N = 446 009 in SEER areas) was 534.9 in 2005, 540.8 in 2008, 505.0 in 2010, and 416.2 in 2012; rates began decreasing in 2008 and the largest decrease occurred between 2011 and 2012, from 498.3 (99% CI, 492.8-503.9) to 416.2 (99% CI, 411.2-421.2). The number of men 50 years and older diagnosed with prostate cancer, Prostate cancer nationwide declined by 33 519, from 213 562 men in 2011 to 180 043 men in 2012. Declines in incidence since 2008 were confined to local/regional-stage disease and were similar across age and race/ethnicity groups. The percentage of men 50 years and older reporting PSA screening in the past 12 months was 36.9% in 2005, 40.6% in 2008, 37.8% in 2010, and 30.8% in 2013. In relative terms, screening rates increased by 10% (SRR, 1.10; 99% CI, 1.01-1.21) between 2005 and 2008 and then decreased by 18% (SRR, 0.82; 99% CI, 0.75-0.89) between 2010 and 2013. Similar screening patterns were found in age subgroups 50 to 74 years and 75 years and older. Conclusions and Relevance: Both the incidence of early-stage prostate cancer and rates of PSA screening have declined and coincide with 2012 USPSTF recommendation to omit PSA screening from routine primary care for men . Longer follow-up is needed to see whether these decreases are associated with trends in mortality.
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1579_biomrc
|
Title: What is a clinically relevant difference in MDADI scores between groups of XXXX @entity1 ?
OBJECTIVE/HYPOTHESIS: To describe clinically relevant between-group differences in MD Anderson patients282 Inventory (MDADI) scores among patients35 ( patients35 ) patients . STUDY DESIGN: Retrospective cross-sectional study was conducted in 1,136 patients35 patients seen for patients282 ( patients282 ) studies. METHODS: The MDADI was administered by written questionnaire at the patients282 appointment. MD Anderson patients282 Inventory global, composite, and subscale scores were calculated. Anchor-based methods were employed to determine clinically meaningful between-group differences by feeding tube status, aspiration status (per patients282 study), and diet level. RESULTS: Mean MDADI scores for the 1,136 patients were: emotional 65.8 17.3, functional 68.1 19.6, physical 60.1 18.6, global 59.3 28.3, and composite 64.0 17.1. Three hundred seventy-eight patients (33%) were feeding tube-dependent; 395 (34.8%) were aspirators; 122 (11%) were nothing per oral (Performance Status Scale-Head and Neck [PSS-HN] diet = 0); and 249 (22%) ate unrestricted, regular diets (PSS-HN diet = 100). Statistically significant (P < 0.0001) between-group differences (feeding tube vs. no feeding tube, aspirator vs. nonaspirator, oral vs. nonoral diet, PSS-HN diet levels) were observed for all mean MDADI scores (global, composite, and subscales). A mean difference of 10 points in composite MDADI scores differentiated feeding tube-dependent from nontube-dependent patients , aspirators from nonaspirators, and distinct PSS-HN diet levels. CONCLUSIONS: We identify that a 10-point between-group difference in composite MDADI scores was associated with clinically meaningful between-group differences in swallowing function. LEVEL OF EVIDENCE: 4. Laryngoscope, 126:1108-1113, 2016.
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1582_biomrc
|
Title: The prevalence of @entity344 in XXXX across the United States with @entity72 from families with multiple affected members.
OBJECTIVE: : To perform a large registry-based study to determine the relative prevalence of gastrointestinal (GI) problems in children with an ASD, autism spectrum disorder ( ASD, autism spectrum disorder ) from families with multiple affected members compared with their unaffected sibling(s). METHODS: : In-home structured retrospective medical history interviews by parent recall were conducted by a pediatric neurologist. Our analysis sample included information about GI health of 589 subjects with idiopathic, familial ASD, autism spectrum disorder and 163 of their unaffected sibling controls registered with autism, Autism Genetic Resource Exchange. Individuals with ASD, autism spectrum disorder were subgrouped into 3 autism, Autism severity groups (Full autism, Autism , Almost autism, Autism , and Spectrum) based on their autism, Autism Diagnostic Interview-Revised and autism, Autism Diagnostic Observation Scale scores. RESULTS: : Parents reported significantly more GI problems in children with ASD, autism spectrum disorder (249/589; 42%) compared with their unaffected siblings (20/163; 12%) (p < .001). The 2 most common Gl problems in children with ASD, autism spectrum disorder were children142 (116/589; 20%) and chronic diarrhea (111/589; 19%). Conditional logistic regression analysis showed that having Full autism, Autism (adjusted odds ratio [AOR] = 14.28, 95% confidence interval [CI]: 6.22-32.77) or Almost autism, Autism (AOR = 5.16, 95% CI 2.02-13.21) was most highly associated with experiencing GI problems . Increased autism, Autism symptom severity was associated with higher odds of GI problems (AOR for trend = 2.63, 95% CI: 1.56-4.45). CONCLUSIONS: : Parents report significantly more GI problems in children with familial ASD, autism spectrum disorder , especially those with Full autism, Autism , than in their unaffected children . Increased autism, Autism symptom severity is associated with increased odds of having GI problems .
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3634_biomrc
|
Title: EFFECTS OF COMPRESSION ON LYMPHEDEMA DURING RESISTANCE EXERCISE IN XXXX WITH BREAST CANCER-RELATED LYMPHEDEMA: A RANDOMIZED, CROSS-OVER TRIAL.
The use of compression garments during exercise is recommended for women with BCRL, breast cancer-related lymphedema , but the evidence behind this clinical recommendation is unclear. The aim of this randomized, cross-over trial was to compare the acute effects of wearing versus not wearing compression during a single bout of moderate-load resistance exercise on lymphedema status and its associated symptoms in women with BCRL, breast cancer-related lymphedema ( BCRL, breast cancer-related lymphedema ). Twenty-five women with clinically diagnosed, stable unilateral BCRL, breast cancer-related lymphedema completed two resistance exercise sessions, one with compression and one without, in a randomized order separated by a minimum 6 day wash-out period. The resistance exercise session consisted of six upper-body exercises, with each exercise performed for three sets at a moderate-load (10-12 repetition maximum). Primary outcome was lymphedema , assessed using bioimpedance spectroscopy (L-Dex score). Secondary outcomes were lymphedema as assessed by arm circumferences (percent inter-limb difference and sum-of-circumferences), and symptom severity for women58 , women1728 and tightness , measured using visual analogue scales. Measurements were taken pre-, immediately post- and 24 hours post-exercise. There was no difference in lymphedema status (i.e., L-Dex scores) pre- and post-exercise sessions or between the compression and non-compression condition [Mean (SD) for compression pre-, immediately post- and 24 hours post-exercise: 17.7 (21.5), 12.7 (16.2) and 14.1 (16.7), respectively; no compression: 15.3 (18.3), 15.3 (17.8), and 13.4 (16.1), respectively]. Circumference values and symptom severity were stable across time and treatment condition. An acute bout of moderate-load, upper-body resistance exercise performed in the absence of compression does not exacerbate lymphedema in women with BCRL, breast cancer-related lymphedema .
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3636_biomrc
|
Title: Great Ormond Street Hospital for XXXX Registry for @entity8205 : prospective study 1988-2007. Part 2--Evaluation of treatments.
BACKGROUND: The treatment of congenital melanocytic naevi (CMNs) has become controversial as better data on complications have been published. OBJECTIVES: To determine the longer-term risks and benefits of surgery in treatment of CMNs. METHODS: In this 19-year prospective study, 301 families completed yearly questionnaires about treatments and CMN changes. Forty per cent of CMNs were > 20 cm projected adult size (PAS) or multiple CMNs. RESULTS: patients, Girls were more likely to have had surgical treatments. There were no significant effects of treatment on the incidence of adverse clinical outcomes, although the numbers for melanoma were small. The majority of untreated CMNs lightened spontaneously during the follow-up period. Surgical treatment and satellites at birth were independently significantly associated with reported darkening of the CMN over the follow-up period. However there was no absolute measurement of final colour. Surgical treatment was associated with decreasing hairiness of the CMN over the follow-up period. PAS was associated with increasing hairiness . Excision with tissue expanders and PAS were significantly associated with an increased incidence of new satellite lesions. A proportion of patients, Girls reported new pigmentation in previously unaffected skin at the edge of a treated area, the majority after complete excision. There was a high level of satisfaction with surgery in the < 20 cm group and in those with facial CMNs. This was significantly reduced with increasing PAS. CONCLUSIONS: There is no evidence here that surgery reduces the incidence of adverse clinical outcomes in childhood. The natural history of the majority of untreated CMNs is to lighten spontaneously, whereas some treatments may cause adverse effects.
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