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Our group explored this process’ advantages to both deoxygenated DX and produced long carbon-chain hydrocarbons. An initial screening of catalysts on a fixed bed reactor concluded that the catalysts based on Pd supported on zeolites, such as Pd/HBEA and Pd/HZSM-5, presented more selectivity to convert DX into hydrocarbons and less coke yield. From these initial results, Pd/HZSM-5 was selected and applied to DX HDO on a batch reactor. Furthermore, a mesoporous Pd/ZSM-5 was explored, which enhanced the hydrocarbons’ yield with seven or more carbons compared with normal HZSM-5. In recent works, two catalysts, Cu-Pd/HZSM-5 and Cu-Pd/HBEA [both containing 0.01% Cu(w/w) and 0.5% Pd (w/w)], were studied as a catalyst for HDO of 15% (w/w) DX in n-hexane for 24 h at 20°C under 40 bar of H2. Both catalysts converted DX into hydrocarbons containing up to ten carbon atoms, such as trimethyl-benzene, xylenes, branched, linear paraffin, and cycloalkanes. More importantly, Cu-Pd/HBEA catalyst yielded more cyclic hydrocarbons when compared with Cu-Pd-HZSM-5. DX has only 11 carbons; in contrast, large compounds (8–30 carbon-chain) are presented in BP. Thus, a further advantage of the mesoporous area is expected in converting large molecules.
When considering the association between MLR, NLR, PLR and number of metastatic sites in the MBC_main cohort, only MLR was significantly associated with number of sites at the onset of metastatic disease (p = 0.0096); on the other hand, NLR was associated with an increase in number of sites (p = 0.036). PLR was associated neither with tumor burden, nor with their increase. Notably, no direct associations were observed between LRs and specific metastatic sites.
We have moved analysis of quartile differences to a new Figure 6 and added data for the middle quartiles to Figure 6D. We note that in Figure 6E all quartiles are plotted for each strain against each quartile's lifespan. In both panels 6D and 6E, a continuous trend is observed in the change in decline rate from shortest-lived to longest-lived worms.
Najbardziej niepokojący dla nas jest rejestrowany w 2018 roku wybuch epidemii odry na Ukrainie. W ciągu pierwszych dwóch tygodni tego roku zanotowano 1285 przypadków choroby, w tym 856 zachorowań wśród dzieci. W 2017 roku stwierdzono na Ukrainie 4782 zachorowania na odrę, w tym 5 zgonów .
We evaluated the performance of different customized CNNs and a VGG16 model in detecting pneumonia and furthermore distinguishing between bacterial and viral types to facilitate timely and accurate disease diagnosis. We evaluated the performance of three different customized CNN architectures: (i) Sequential CNN; (ii) CNN with residual connections (Residual CNN); and, (iii) CNN with Inception modules (Inception CNN).
Trx1 levels in serum of MI and sham-operated rats at different times after surgery. Trx1 levels quantified by ELISA at day 2, day 8, and day 15 after MI or sham operation. Individual values of eight animals per group are shown. Mean ± SD shown for each group. Asterisk indicates significant difference (P = 0.0085)
The first indication that alpha-herpesvirus proteins promote de-envelopment involved a PRV mutant lacking that US3 gene that accumulated PEVs . These enveloped virions were not distributed throughout the perinuclear space but were found in herniations of the inner nuclear membrane , similar to the herniations observed later with gB−/gH− double mutants (see examples in Figure 2). US3 mutations in other, distantly related alpha-herpesviruses also produced similar defects in de-envelopment . Therefore, pUS3 is important for efficient de-envelopment of alpha-herpesvirus capsids at the ONM.
Ultimately, we foresee development of a closed-loop prosthesis system with multiple discriminable sensory percepts coupled to sensors that span a physical prosthetic hand for use in activities of daily living. We anticipate that discriminable sensory feedback via a prosthesis will enhance motor control, particularly in scenarios where visual feedback is limited or undesired. Also, we anticipate that discriminable, multi-sensor feedback with variable intensity and tunable quality will further enhance the level of embodiment of a prosthetic limb, helping amputees to feel as though their prosthesis is a replacement hand, in addition to being a useful tool. Sensory feedback during closed-loop control, and any associated limb embodiment, may also alleviate phantom pain and many of the psychological difficulties associated with losing a hand [3, 20, 23, 25].
In conclusion, we observed altered central autonomic connectivity in patients with TTS both at rest and in response to a cold challenge. These findings may underlie the increased sensitivity of these subjects to acute heart failure when exposed to stressful triggers.
The establishment model calculates how many infected Vaccinium plants result from entry through the six pathways considered in the entry stepper year. The establishment model consists of two steps: (a) distribution of the import of infected fruit and plants for planting (P4P) over EU countries in four main regions (NW, NE, SW and SE – Figure 4), and (b) establishment in those regions based on suitability of the climate for D. vaccinii in production areas and both climate suitability and Vaccinium plant density in natural areas. These established infected plants are the starting point for spread within the EU.
PIP5KI is also involved in HIV-1 entry, through actin cytoskeleton remodeling, and in assembly at the PM. HIV-I assembly occurs at the PM, but at different sites in T cells and macrophages. The factors governing this phenomenon include differences in the cellular distribution of PIP5KI isoforms and, thus, of PI(4,5)P2 between CD4+ T cells and macrophages. Further studies of the isoforms of PIP5KI and the metabolism of PI(4,5)P2 are required to test confirm this hypothesis. The viral envelope is derived from the PM of the infected cell and the composition of the PM differs between cell types. The lipid composition of the viral envelope therefore differs between cell types. These differences may explain the difference in the location of HIV-1 assembly between T cells and macrophages , and the differences in the distribution of PPIns. Some of the effects of PIP5KI on HIV-1 assembly have been deciphered in T cells, but the role of PIP5KI in HIV-1 assembly in macrophages requires further characterization. Taken together, these results suggest that these PI kinases are potentially useful therapeutic targets for blocking viral replication. However, they are also crucial elements for the correct functioning of the cell, so caution will be required in the development of therapeutic approaches targeting them.
El aumento descontrolado de la población canina causa un impacto negativo en la salud pública 60. Los perros callejeros representan un factor de riesgo de infección para las personas y para otros animales 32,60,61. Las enfermedades en perros con tutores responsables son relativamente más fáciles de controlar, en comparación con la población callejera, puesto que esta deambula por la calle y tiene más probabilidades de entrar en contacto con otros perros infectados y luego convertirse en potenciales diseminadores del agente patógeno 32. Por lo tanto, las estrategias de diagnóstico no deberían excluir las poblaciones de perros callejeros, con el fin de determinar la prevalencia e implementar las medidas pertinentes de control de B. canis en estos grupos.
Analysis of genomic ancestry was conducted using the ADMIXTURE program . ADMIXTURE is a software tool for maximum likelihood estimation of individual ancestries from multilocus SNP genotype datasets. Specifically, Admixture uses a block relaxation approach to alternately update allele frequency and ancestry fraction. ADMIXTURE estimates parameter standard errors using bootstrapping. As the contributions of differential ancestral genomes have previously been described by our group, as well as others, we used a supervised approach for ancestry determination. We used 200 bootstrap replicates (default) and k = 3 (number of populations assumed for the analysis). All ADMIXTURE analyses were repeated 4 times with different random seed numbers and in all cases, results were highly correlated.
В 2012 г. впервые выполнен транскриптомный анализ для совместимых и несовместимых взаимодействий P. infestans и S. tuberosum (Gyetvai et al., 2012). Исследовано пять изогенных линий, которые различались только наличием и отсутствием R-гена (R1), с целью исключить влияние генетического фона на транскриптом. Однако оказалось, что изменения транскриптома между растениями одной линии отличаются больше, чем средние транскриптомы линий между собой, что указывает на значительное влияние как индивидуальных физиологических параметров, так и факторов окружающей среды в процессе защитной реакции. Одним из них может быть движение во времени и пространстве защитных сигналов от места первоначального физического контакта между зооспорами P. infestans и клетками-хозяевами и соседними клетками. Анализ DeepSAGE позволил выявить некоторые интересные аспекты общей структуры транскриптома. Две трети (68 %) унитагов были экспрессированы на низких уровнях (<10 CPM) в ткани листьев, из которых одна треть (36 %) не соответствовала ни одному из известных транскриптов картофеля, что может быть объяснено немодельным объектом и немногочисленными данными о транскриптоме S. tuberosum. С другой стороны, только 3 % унитагов показали среднюю экспрессию выше 100 CPM, но составили 32 % от общего транскриптома. Из очень частых транскриптов только 14 % были не известны. Нужно отметить следующие транскрипты из этого исследования:
La incidencia de afectación de la médula ósea en pacientes con esta condición es de alrededor del 34 %. La mayoría de los pacientes con afectación de la médula ósea son niños pequeños con enfermedad difusa en el hígado, el bazo, los ganglios linfáticos y la piel 11. Dicha manifestación puede darse como neutropenia, anemia y trombocitopenia en el 15 al 30 % de los casos 4. En el presente caso, se determinó compromiso de la medula ósea por la presencia de neutropenia, lo que complicó el estado clínico del paciente al detectarse bacteriemia por S. aureus.
Firesetters differ from other groups of children on school and academic performance in the few studies conducted utilizing school information. Firesetters and delinquent control groups are shown to have “poor academic performance, history of grade failure, and truancy” [Ref. (12), p. 498]. Firesetting is a specific type of delinquent behavior; and, therefore it is not surprising that children who set fires have similar struggles in school as their delinquent peers.
The Galvanic series, Figure 25, is more appropriate than the EMF series for description of actual corrosion problems as alloys and not pure metals are often coupled, and the metals are undergoing corrosion rather than being in equilibrium with their ions at standard concentrations. Usually, the further apart two metals are in the series (i.e., the greater the potential difference between them), the greater is the driving force for galvanic corrosion and the greater the damage to the anode will often be . Often, the maximum allowable potential difference between the metals is 200 mV.
The cytoskeleton is a kind of protein fiber network structure and widely exists in eukaryotic cells, which maintains cell morphology and participates in cell movement, cell polarity, cell division, and signal transduction (11). PDLIM1 is a cytoskeletal protein, also known as CLP36, belonging to the PDZ and LIM protein family. Mediated via the PDZ domain, PDLIM1 binds to alpha-actinin and is localized to actin stress fibers in the cytoplasm (12). Many studies demonstrated that PDLIM1 plays a role in the regulation of actin cytoskeleton organization in non-muscle tissues (12–14). Studies indicated that the abnormal expression of PDLIM1 is associated with hepatocellular carcinoma, breast cancer, colorectal cancer, and pancreatic cancer (15–18). In Liu’s study, PDLIM1 was reported to promote breast cancer cell migration and invasion in vitro and metastasis in vivo through interaction with α-actinin (17). During metastasis of hepatocellular carcinoma (HCC), PDLIM1 was demonstrated to play an important inhibitory role through activating the Hippo signaling pathway (16). It seems that PDLIM1 plays different regulatory roles in different kinds of cancer cells (16, 17).
Wiederholt konnte im zeitlichen Abstand von drei Wochen bis 10 Monaten nach Erkrankungsbeginn das Auftreten eines Diabetes mellitus beobachtet werden . Ob es sich um eine transiente Stoffwechselstörung oder eine lebenslange Folgeerscheinung handelt, ist aktuell aufgrund fehlender Langzeituntersuchungen ungewiss. Das therapeutische Konzept sollte zur Vermeidung bzw. frühzeitigen Behandlung von möglichen Folgeerscheinungen regelmäßige Verlaufskontrollen einschließen. Kontrollen von Blutzucker- und Schilddrüsenwerten sowie Leber- und Nierenfunktionsparametern sind ebenso zu empfehlen wie Blutbild‑, Leber- und Nierenwertkontrollen [7, 21].
En las investigaciones mencionadas y en el presente estudio, el desempeño de la prueba fue satisfactorio y el vehículo de dilución y los demás elementos del medio de cultivo no afectaron la acción antimicrobiana del aceite del árbol de té, lo que resultó en un crecimiento satisfactorio de C. acnes en las placas de Petri (control de crecimiento en el medio de cultivo y control de crecimiento en el vehículo de dilución) y en ausencia de crecimiento en el control negativo, lo que garantiza la calidad, la funcionalidad y la confiabilidad del método para evaluar la actividad antimicrobiana.
Biochemical parameters are shown in Table 1. Serum calcium was within the normal range with no significant difference between summer (June to August) and winter (December to February), respectively, with a mean level of 2.36 ± 0.06 mmol/l vs 2.32 ± 0.1 mmol/l ( P = 0.16). Only one patient had high serum calcium (>2.6 mmol/L), (without hypercalciuria) related to a systemic form with documented bone sarcoidosis lesions. Nine out of 94 patients presented hypercalciuria (24-hour urinary calcium >0.1 mmol/kg).
This provides a list of the most common keywords in these two journals, and presumably in evolutionary biology. As some of the most common keywords are not very specific or informative (e.g. evolution, selection, adaptation or body size), these were excluded. This left a list of the following 10 keywords (in order of their relative frequency): 1. Sexual selection; 2. Speciation; 3. Natural selection; 4. Inbreeding depression; 5. Reproductive isolation; 6. Phenotypic plasticity; 7. Gene flow; 8. Phylogeny; 9. Local adaptation; 10. Mate choice. These ten keywords covered about two-thirds of all articles published in Evolution and Journal of Evolutionary Biology.
Moreover, we analyzed BolA family members’ protein expression in normal ovarian tissues and OC tissues using the HPA database. As shown in Fig. 3, we found that ovarian stroma cells had medium BOLA1 staining in 3 cases of normal ovarian tissues. Relatively, among 11 cases of OC tissues examined, 2 cases had medium BOLA2 staining, 5 cases had low BOLA2 staining, and 4 cases had no BOLA1 staining. For BOLA2, it was undetected in normal ovarian tissues, however, among the examined 12 OC tissues, 9 cases had medium BOLA2 staining, 1 case had low BOLA2 staining, and 2 cases had no BOLA2 staining. For BOLA3, the data showed no BOLA3 staining in normal ovarian tissues. In comparison, among 11 cases of OC tissues examined, there were 2 cases of mediumBOLA3 staining, 3 cases of low BOLA3 staining, and 6 cases of no BOLA3 staining. Fig. 3The protein expression profile of BOLA family members in regular ovarian compared with cancer tissues. Representative immunohistochemistry images (a) and expression status (b) of BOLA family members in normal ovarian tissues and ovarian cancer tissues based on HPA database (http://www.proteinatlas.org/)
Moreover, P. aeruginosa, E. cloacae, E. aerogenes, and M. haemolytica were detected (Table 3) more frequently from diseased calves compared to the healthy calves. Previous studies recovered Bacillus species, Klebsiella species and P. aeruginosa in calves with bronchopneumonia (de Oliveira et al. 2016). Regarding pathogens, some species that are not of obvious importance associated with pneumonia, but their opportunistic role should not be ignored (Griffin et al. 2010).
A) I–V plots of DCEBIO current recorded from mast cell of control animals. The current was fitted with the GHK equation (O) and PK for this cell was determined to be 5.1×10−6 cm3/s. B) DCEBIO current recorded from mast cell of L.rhamnosus JB-1 fed animal was substantially smaller than that from control animals. For this representative cell PK = 1.3×10−7 cm3/s. C) Summary data for DCEBIO current experiments given as mean ± SEM. Feeding L.rhamnosus JB-1 instead of culture medium substantially blocked the DCEBIO current (P = 0.0001). D) Two superimposed traces of I-V plots, the first was performed only Krebs buffer as the perfusate and the second was made when 1 µM DCEBIO was added in the presence of 5 µM TRAM-34. DCEBIO had no effect in the presence of the IKCa channel blocker TRAM-34, cells were obtained from at least 4 animals in each treatment group, *** = p<0.001.
The respiratory tract, as the first barrier against the invasion of pathogens, is also a place for pathogens to replicate and move.[ 7 ] SARS‐CoV‐2 can be detected in human upper respiratory tract samples, including nasopharyngeal and oropharyngeal specimens,[ 8 ] and is spread through respiratory droplets. The virus enters the host cell by binding its spike protein to angiotensin‐converting enzyme 2 (ACE2) on human cells.[ 9 ] TMPRSS2 helps to activate the spike protein on SARS‐CoV‐2.[ 10 ] After entering host cells, the virus can not only cause inflammation and immune disorders but also destroy the upper respiratory tract microbial balance.
Concerning the measles vaccination strategy of the other 11 participants, 3 main approaches emerged: (i) delayed administration of the MCV, but before kindergarten entry, (ii) a delay of the MCV until puberty (the time when the risk of complications increase) or (iii) complete rejection of the MCV. Supporters of the first approach aimed to protect children from getting measles, but preferred the delay of the vaccine due to a supposed enhancement of immune response and a decrease in side effects. Supporters of the second and third approach pursued the target of acquiring the infection due to perceived benefits of the disease. Dr Huber (♀, 46 years, P, HP) was ‘not convinced that the shots should be given at nine months’, as studies would indicate that ‘the earlier children get vaccinated, the less antibody levels rise’. In the same manner, several participants criticized that early vaccination would lead to weak immunity levels among the whole population and therefore to an impaired transferral of maternal antibodies to newborns. This would result in decreased nest protection of infants and to an increased susceptibility of adults, who lose their initially weak protection. Dr Bauer (♀, 42 years, P, anthroposophic medicine) who shared this view explained that for that reason she was ‘not sure if it was a right public health decision to introduce the measles vaccine’. Dr Wolf (♀, 43 years, P, HP) also applied this approach to her own children: ‘Well, I just took the chances and let as much time as possible pass by to give them the chance to develop a natural immunity by catching their diseases. And if they do not catch a disease till adolescence, I vaccinate them’.
Por último, la expresión de los genes se evaluó mediante qPCR utilizando una concentración de 1 ng/µl para todas las muestras de ADNc dentro del rango dinámico estandarizado, con una eficiencia de amplificación del SNCA de 2,18, y los iniciadores de diseño propio F: GCCAAGGAGGGAGTTGTGGCTGC y R: TGTTGCCACACCATGCACCACTCC, en una concentración de 0,125 µM, mediante la herramienta primer-blast disponible en http://www.ncbi.nlm.nih.gov/tools/primer-blast.
行TBNA时每个淋巴结穿刺几次能获得较高的阳性率，文献报道差异较大。有研究[16, 22]推荐3次，有研究建议当穿刺仅仅是为了明确诊断，且在一个以上淋巴结穿刺取材或有其他的取材手段时，3针较合适，而当穿刺目的是肺癌的分期时应至少穿刺4针-5针。最近一篇系统性回顾中推荐3针-5针。但上述研究均不是专门针对肺癌患者。主要针对肺癌患者的一项前瞻性研究中发现穿刺4针-7针可获得最好的诊断率，穿刺7次诊断率达到一个平台，再增加穿刺次数阳性率无明显增加。同时该研究中发现现场细胞学组的平均穿刺次数为（2.5±2.0）次，非现场细胞学组穿刺次数为（3.7±1.6）次，两者相比差异有统计学意义，因此得出现场细胞学能减少穿刺针数的结论。我们的研究中现场细胞学组平均穿刺次数为（2.4±0.2）次、非现场细胞学组平均穿刺次数为（4.6±0.4）次，所得结论与上述研究相一致。同时我们的研究中发现因穿刺针数减少，病理科医师阅片张数减少，因此细胞学诊断费用下降，同样因为穿刺针数减少，应用现场细胞学组出血发生率较未应用现场细胞学组减少，这与Trisolini等的研究结论一致。
1. La prevalencia de Prediabetes en Colombia es alta y a pesar estar reconocida y categorizada en las principales Guías Médicas de la especialidad nacionales e internacionales, y de estar incluida en la Clasificación Internacional de Enfermedades (CIE-10) vigente en Colombia, el conocimiento que de ella tiene el equipo de salud y la comunidad es limitada, por lo que se recomienda que las Facultades de Ciencias de la Salud y las Asociaciones Científicas relacionadas con la educación médica continuada implementen acciones destinadas a mejorar los conocimientos del equipo de salud y de la comunidad en general sobre el significado que tiene el identificar, diagnosticar y tratar al paciente con prediabetes. El grupo de expertos recomienda también que en los programas educativos que deben implementarse se insista en la necesidad de un enfoque global del riesgo incluyendo la importancia de conocer y evaluar otros factores de riesgo como antecedentes familiares de DM2, sobrepeso y obesidad, dislipidemia e hipertensión. El Ministerio de Salud debe hacer evidente el código de la prediabetes y divulgarlo para que la enfermedad pueda aparecer como un diagnóstico específico en las historias clínicas de los pacientes.
For the CVD positive (CVD+) and CVD negative (CVD−) group, age‐adjusted odds ratios (aORs) were calculated using multiple logistic regression analysis to determine which risk factors may have an impact on target achievement. Adjustment was made for age as age is a confounder for the achievement of HbA1c, LDL‐c, and blood pressure. 23 aORs were calculated for the following risk factors: sex, continuous subcutaneous insulin infusion (CSII), BMI, microvascular complications, smoking, eGFR, lipid‐lowering medication (LLM), and antihypertensive medication (AHM). Diabetes duration was excluded as diabetes duration and age were highly correlated.
Material: Raportăm un caz al unei femei de 68 ani aflată la menopauză postanexectomie cu istoric de numeroase căderi de la propria înălțime, care se internează pentru durere având caracter mecanic la nivelul genunchiului și șoldului drept, dizabilitate funcțională la mers. APP semnificative: cancer col uterin, DZ tip 2 insulinonecesitant, HTA, sindrom mielodisplazic cu anemie secundară și istoric de numeroase fracturi prin căderi de la propria înălțime: 2003-fractură deschisă tibie și peroneu drept diafiză distală, 2009-fractură radius bilateral și 2016-fractură subtrohanterian (trohantero-diafizar) femur drept, fractură trohanteriană de femur drept și fractură de bazin în 2013 prin accident rutier. Este diagnosticată ulterior accidentului rutier, în 2013, cu osteoporoză (DXA: T score L1-L4=-3,3 si sold stg=-2,2) și primește tratament cu Bonviva 150mg 1cp/lună, alpha D3 0,5 mcg 1cp/zi, Ca lactic 500mg timp de 3 ani când DXA a indicat osteopenia.
Schaltet ein Angreifer eine Gehirn-Computer-Schnittstelle aus oder zerstört ihre Funktionsfähigkeit,99 beeinträchtigt das ihren Nutzer in seinem Eigentumsrecht (Art. 14 Abs. 1 GG).100 Das Gleiche gilt, wenn Cyberangriffe die Gehirn-Computer-Schnittstelle mit Anfragen überlasten, bis sie ihre Funktionen nicht mehr erfüllen kann.101 Auch in diesen Fällen entzieht der Angreifer dem Eigentümer die Nutzungsmöglichkeit.
Niniejsza publikacja nawiązuje do wcześniejszych ogólnokrajowych analiz umieralności niemowląt i noworodków, zdrowia matek oraz opieki okołoporodowej prowadzonych przez Instytut Matki i Dziecka od ponad 50 lat a następnie artykułów pierwszej autorki na ten temat i prac badawczych wykonywanych przez nią w ramach projektu Unii Europejskiej Peristat [2, 3, 4]. Opracowanie otwiera cykl poświęcony zagadnieniom szeroko rozumianej epidemiologii perinatalnej. Przedstawiono tu, między innymi dynamikę cząstkowych współczynników umieralności według czasu trwania ciąży. Dotychczas, w naszych publikacjach współczynniki cząstkowe prezentowane były według grup masy urodzeniowej. Wydaje się jednak, że jakość danych dotyczących czasu trwania ciąży w zgłoszeniach urodzenia noworodka (tzw. najlepsze oszacowanie położnika) jest obecnie na tyle wysoka, że czas trwania ciąży może być uwzględniany w analizach umieralności niemowląt i noworodków. To bowiem czas trwania ciąży a nie masa urodzeniowa per se świadczy przede wszystkim o dojrzałości noworodka i determinuje jego szanse przeżycia poza organizmem matki.
Eleven studies [31, 35, 36, 40, 41, 48, 50, 52, 54, 55, 61] identified trunk biomechanics as a biomechanical determinant responsible for generating large KJLs namely KAMs, or targeted the trunk in an intervention with the view to reducing KJLs and are presented in Table 2. Being the largest segment of the body, containing approximately half of the body’s mass, trunk positioning plays a significant role regarding the direction of the GRF vector during the weight acceptance phase of the cut. A contralateral lean of the trunk in the opposite direction of travel results in a greater laterally directed GRF . This results in a greater perpendicular distance between the GRF and the knee, and increases KJLs due to a greater moment arm of the GRF in the frontal plane. Numerous other studies retrieved identified lateral trunk flexion as being a significant contributor to KJLs in pre-planned [36, 48, 55, 61] and unplanned [35, 52, 54] cuts of varying magnitudes (Table 2). Table 2Studies whereby trunk motion has observed to be contributory to KJLsStudyParticipantsCuts usedFindingsChaudhari et al., 2005 11 healthy subjects (6 F, 5 M)4 m approach 90° PP cut unconstrained, and three sport-specific conditions including holding a lacrosse stick and football in the cutting side arm, and a football in the non-cutting side arm.Constraining plant side arm with lacrosse stick and football result in ↑KAM of 60% and 29% respectively (p = 0.03)Dempsey et al., 2007 15 M healthy team sports athletesPP 45°cut modifying different technical parameters such as foot-plant distance, contralateral trunk lean and foot progression angle↑ Foot-plant distance and contralateral trunk lean resulted in ↑ KAMs (p < 0.0001 and 0.030 respectively)Dempsey et al., 2009 12 M healthy team sports athletesPP and UP 45° cuts6 weeks technique modification significantly ↓ in FP distance (p = 0.039 PP (ES = 0.55), UP (ES = 0.5)) and torso LF (p = .005 PP (ES = 1.09), UP (ES = 0.14)) leading to 36% ↓ in KAMs (p = 0.034; PP (ES = 0.58), UP (ES = 0.78)).Both postural changes were correlated with the change in KAM: FP distance ( r = -0.468, p = 0.025), LTF (r = − 0.377, p = 0.135)Jamison et al., 2012 29 (15 M, 14 F) healthy subjectsThree steps at a self-selected pace UP 45° cutTorso angle (outside tilt = (p = 0.02)) and torso GRF shoulder angle (p = 0.036) ↑ KAMs.Jamison et al., 2012 36 M high school American footballersThree steps at a self-selected pace UP 45° cut6-week TS programme elicited no reduction in KAMs (p = 0.116)Jamison et al., 2013 46 (23 M, 23 F) healthy subjectsThree steps at a self-selected pace UP 45° cutAll co-contraction indices and avg%diff of IO EO and L5 not significantly associated with KAMs or contralateral trunk lean p = 0.741, 0.782 and 0.233 for KAMs and p = 0.419, 0.947 and 0.439 for LTF.Jones et al., 2015 26 elite and sub-elite F footballers10 m approach 3 m exit of PP 90° cutLTF sig correlated to KAM (R = − .42, R2 = 18; p = 0.05)Kristianslund et al., 2014 123 F handball playersHandball-specific protocol—self-selected cut when receiving a ball and cutting around a static defender (mean cutting angle = 67°)1 SD (8.6°) ↑ in LTF results in 7% ↑ KAMLTF significant predictor of pKAM (ß = 0.0090, p < 0.001) and moment arm of GRF at time of pKAM (ß = 0.00032; p < 0.001)Mornieux et al., 201 13 M amateur footballers4 m approach PP and UP 45 cuts 850-, 600- and 500-ms delays in stimulus presentation for UP cutsLTF and KAM ↑ with reduced preparation time PP and 800 ms vs 500 ms (p = 0.05)LTF sig correlated with KAM (r = 0.41; p = 0.009)Weir et al., 2019 30 F hockey players (15 junior, 15 elite)UP 45° cutting taskLinear regression revealed that LTF and TF sig predictors of KAM (p = 0.05)Weltin et al., 2017 28 F elite and sub-elite team sports athletesUP 45° cutting taskPPT ↓CTR (p = 0.008, η2 = 0.277), step width (p = 0.029, η2 = 0.199) and ↑ pelvic axial rotation (p = 0.049, η2 = 0.165). No ↓ in KAMs (p = 0.605)Avg%diff average percentage difference, CTR contralateral trunk rotation, TF trunk flexion, TS trunk stabilisation, IO internal obliques, EO external obliques, KAM knee abduction moment, L5 L5 extensors, LTF lateral trunk flexion, FP foot plant, ms milliseconds, PPT perturbed plyometric training, η2 partial eta squared, PP pre-planned, UP unplanned, SD standard deviation, M male, F female, ROM range of motion, ß beta value, ↑ increased, ↓ decreased, ES effect size
Na avaliação da reprodutibilidade na análise eletrocardiográfica, o nível de concordância entre os dois observadores foi de 0,82 e 0,94 (índice de kappa), considerados excelentes. O primeiro valor corresponde à duração do complexo QRS e o último à concordância dos critérios eletrocardiográficos. Foram construídas curvas ROC (Receiver Operating Characteristic), para os três grupos estudados ( Figura 2 ).
Male BALB/cAnN.Cg nude mice (age: 4–6 weeks; wt: 18–20 g) were purchased from the National Laboratory Animal Breeding and Research Center, Taipei, Taiwan. The mouse xenograft experimental procedures conducted in this study were approved by The Institutional Animal Care and Use Committee of Kaohsiung Chang Gung Memorial Hospital (IACUC No. 2014121610). BALB/cAnN.Cg nude mice were housed in pathogen-free conditions and experimental procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals under the supervision of authorized investigators. FaDu cells (5 × 106) were inoculated subcutaneously into the back of nude mice under 2–4% isoflurane inhalational anesthesia. Tumors were measured in length and width using calipers every 2–3 days for 3 weeks. Mice were assigned randomly to receive NVP-BEZ235 (oral administration: 50 mg/kg/day for 3 weeks), Cisplatin (intraperitoneal injections: 3.5 mg/kg/day twice a week for 3 weeks), a combination of the two at the same dose and schedule, or placebo for negative control. Tumor material was harvested for immunohistological studies by formalin fixation.
Juntamente com a ecocardiografia, a ventriculografia radioisotópica é o método mais amplamente aceito para avaliar a FEVE de pacientes antes e durante tratamento do câncer na identificação do risco para IC crônica (90% de sensibilidade e 72% de especificidade). 49
These results constitute the first irrefutable evidence that coating of nanoparticles with specific polymers induces the rupture of the endo-/lysosomes and further escape to the cytosol. Both fluorescence and electron microscopy images demonstrate an evident enhancement/acceleration of the endosomal escape efficiency with HA-PEI coating compared to using PEI alone. Further research is necessary to assess the mechanism behind this effect, although we speculate that it might be associated either to a faster uptake rate of the HAPEI-coated particles, thanks to the HA targeting, or, more generally, to the presence of an additional polymer. At low pH, the inclusion of an extra polymeric layer can, indeed, increase both the buffering capacity and the polymeric swelling, contributing to the destabilization of the endo-/lysosomal membrane43.
Some consumer risk appraisal patterns were comparable with explanations provided by psychological theories. Consumer acknowledgement that natural frequencies are easier to understand is supported by FH. Differing consumer interpretation of risk highlights the role of numeracy in how consumers encode and appraise risk information via gist encoding, as explained by FTT.29 High numeracy41 and subjective numeracy50 have been shown to contribute to improved gist understanding. Interestingly, however, even though consumers acknowledged that the information represented a low numerical (verbatim or literal) side‐effect risk, some consumers still regarded it as high risk, which is in line with AH and the role that subjective perceptions have in forming consumer perspectives on risk. Consequently, a plethora of existing factors influenced risk perception.
It is well known that melanin in human hair exists in the form of melanin granules, which was reported to contain plenty of functional groups such as amino, carboxyl, and indolyl groups (Cho et al., 2017, Mostert et al., 2018, D’Ischia et al., 2014). In our previous study, natural melanin particles (NMPs) were extracted from human hair by using alkaline-degradation method, also confirming the presence of functional groups (Zheng et al., 2018). Here, NMPs, extracted from human hairs, were bound with metal ions to mimic the natural enzymes (Scheme 1). On the one hand, the abundant functional groups in NMPs could adsorb substrate and provide active sites for substrate binding as the protein part in natural enzymes. On the other hand, the metal ions in NMPs could also work as the metallic cofactor of enzyme (Scheme 1). It is hoped that the metal-bound NMPs (M-NMPs) derived from human hairs could serve as efficient, stable, and low-cost enzyme alternatives, providing a new view and useful tool for future biomedical applications.Scheme 1Schematic Illustration Showing the Fabrication and Multi-Enzyme Activity of Metal-Bound Natural Melanin Particles (NMPs) for Various Biomedical ApplicationsInspired by natural enzymes, NMPs are extracted from human hair by alkali-heat-treatment and further bound with metal ions to mimic natural enzyme activity.
Pre- and post-intervention values, as well as changes at follow-up based on the allocated groups and training effect in anthropometry and body composition, are shown in Table 3. Our findings showed that FM% at baseline was higher in the EG compared to the CG (p < 0.05, Student’s t test), while FFM% at baseline was lower in the EG compared to the CG (p < 0.05, Mann–Whitney test). No differences at baseline were noticed for the remaining analyzed variables. After the intervention, the results showed that FM% was reduced and FFM% increased only in the EG, and no changes at follow-up were found in the CG for any of the analyzed variables. However, compared with the CG, none of the analyzed variables at the 12-week follow-up changed in the EG.Table 3Effect of the intervention on anthropometric measurementsExperimental (n = 10)ChangeControl (n = 10)ChangeANOVA (interaction)VariablesPrePostpdPrePostpdFpη2 (%)BMI (kg·m−2)&48.7 ± 5.547.8 ± 5.90.071 − 0.8644.2 ± 5.743.9 ± 5.20.540 − 0.280.8370.3724.4Weight (kg)125.3 ± 13.9123.1 ± 14.80.078 − 0.84115.8 ± 15.1114.9 ± 13.90.497 − 0.310.6900.4173.7EBW (kg)60.9 ± 13.658.6 ± 14.40.078 − 0.8450.2 ± 14.449.4 ± 13.20.497 − 0.310.6900.4173.7EBW (%)48.1 ± 5.847.1 ± 6.20.060 − 0.9042.7 ± 6.842.4 ± 6.40.565 − 0.261.0100.3285.3Fat mass (kg)&66.3 ± 8.363.8 ± 8.50.006* − 1.3758.9 ± 8.858.6 ± 8.60.718 − 0.163.7290.06917.2Fat mass (%)52.9 ± 1.851.8 ± 2.20.015* − 1.2050.8 ± 2.450.8 ± 2.50.8390.094.1600.05618.8FFM (kg)58.9 ± 6.359.2 ± 7.20.7820.1356.9 ± 7.556.3 ± 6.30.491 − 0.310.4840.4962.6FFM (%)47.1 ± 1.8&48.1 ± 2.20.0151.2049.2 ± 2.449.1 ± 2.50.839 − 0.094.1600.05618.8Waist (cm)&142 ± 14140 ± 150.334 − 0.44134 ± 16134 ± 150.719 − 0.160.1970.6631.1Hip (cm)147 ± 11146 ± 140.410 − 0.38140 ± 14139 ± 130.633 − 0.220.0640.8030.4WHR (cm)a0.9 ± 0.10.9 ± 0.10.721 − 0.160.9 ± 0.10.9 ± 0.10.929 − 0.040.0370.8500.2Visceral fat (%)&17.8 ± 2.517.2 ± 2.50.013 − 1.2315.6 ± 2.915.3 ± 2.70.189 − 0.610.9440.3440.5BMI body mass index, EBW excess body weight, FFM fat-free mass, WHR waist-to-hip ratio. Data at pre- and post-intervention are delivered as mean ± SD&Data were log-transformed before performing the ANOVAaNon-normally distributed data
Given the morphological changes observed in the MDA-MB-231BR metastatic variant upon hypoglycemic conditions, we screened all cells for their expression of a panel of cell adhesion molecules, which were previously shown to modulate cellular attachment and cell morphology, and influence metastatic ability. These molecules included the epithelial molecules E-cadherin, α, β and γ catenin, and the mesenchymal marker N cadherin ( Figure 5 ). Consistent with previous studies which found that loss of E-cadherin associates with metastatic ability , MDA-MB-231P, MDA-MB-231BO and MDA-MB-231BR cells had completely lost E-cadherin expression ( Figure 5A ). N-cadherin was highly expressed in all the studied cells (only 10 µg of total protein lysate was loaded), and was down regulated in the MDA-MB-231P and MDA-MB-231BO cells under hypoglycemia. The MDA-MB-231BR cells expressed the lowest level of N-cadherin when compared to the other cells ( Figure 5B ; blot on the left and graph on the right of the panel). Expression of the known tumor suppressor γ-catenin was less in the metastatic variant cells when compared to the MDA-MB-231P cells and was also down regulated in hypoglycemia. β-catenin was detected in slightly higher levels in both MDA-MB-231P and MDA-MB-231BO when compared to MDA-MB-231BR, and was not significantly down regulated under hypoglycemia in MDA-MB-231P cells however its expression in both MDA-MB-231BO and MDA-MB-231BR cells was significantly reduced under hypoglycemia (p = 0.0037 and p = 0.0014) ( Figure 5B ; bottom left graph). α-catenin was the least abundant among the three catenins and was detected in slightly higher amounts in the MDA-MB-231P cells, with no significant change in response to glucose concentrations, however, it showed significant reduction under hypoglycemia in both variant cells ( Figure 5B ; bottom graph to the right).
Thin lines indicate data for each individual subject. The thick line indicates the estimated mean value over time from mixed effects linear regression. P-values refer to change from baseline at each referenced time-point. hsCRP = high sensitivity C-reactive protein.
The breeding pair of rTg4510 with strain backgrounds of FVB and 129S6 was licensed from the Mayo Clinic and bred at the National Laboratory Animal Center (NLAC, Taipei Taiwan), an AAALAC-accredited facility. The bitransgenic features of the F1 offspring carrying both CaMK2a-tTA and TetO-MAPT*P301L were determined by PCR genotyping from 10-day-old mouse toes. Both female and male mice were used throughout the study. Animal care was in compliance with the Guide for the Care and Use of Laboratory Animals. All animals were delivered from NLAC to The Division of Laboratory Animal Resources at Chang Gung Memorial Hospital (CGMH, Taoyuan, Taiwan) a few days prior to the surgical procedure for habituation. This study was approved by the Institutional Animal Care and Use Committee (IACUC No. 2017103001) at CGMH.
Genomic sequences for two profilin genes Pru p/du 4.01 and Pru p/du 4.02 were obtained based on their cDNA data [GenBank: EU424270-EU424273]. Both genes have three exons with conserved length, 123, 138 and 135 nt, in agreement with that found in most plant species. DNA polymorphisms in Pru p/du 4.01 occurred mainly in the first intron, but in Pru p/du 4.02 they were in the first and second exon. These two genes gave the same protein sequences for peach and almond (Figure 6), although a few synonymous polymorphisms existed at the gDNA level. Molecular weights were 14.06 and 14.19 kDa for Pru p 4.01 and 4.02, respectively, and both were acidic (calculated pI 4.5 and 4.6). Compared with the six known Mal d 4 isoallergens, Pru p 4.01 was similar to Mal d 4.03A and B (93.1% and 93.9% identity), while Pru p 4.02 was similar to Mal d 4.02A and B (95.4% and 96.9% identity) (Figure 2D). Pru p 4.01 was mapped on G1 and Pru p 4.02 on G7 (Figure 3A and 3D).
To observe the in vivo drug sensitivity, 5 × 105 4T1/WT cells and 4T1/EPB cells were then challenged subcutaneously (s.c.) into the opposite flanks of 6–8-week-old BALB/c female mice, respectively. The mice received an intravenous (i.v.) injection of EPB (200 µg/mouse) or normal saline (NS) in a total volume of 200 µl on day 5 and day 10 (n = 6 per group). All mice survived up to the designed study endpoint and were sacrificed on day 30 under deep anesthesia by an intraperitoneal (i.p.) injection of 150 mg/kg pentobarbital sodium. The tumor tissues were dissected, the tumor was photographed, and the weight of the tumors was measured.
Radiomics can extract texture features that can be recognized by a computer from a large amount of medical image data and combined with relevant data analysis to build a specific clinical model that provides support for clinical decision-making and diagnosis. Radiomics has certain clinical application value in the prediction of tumor gene status. Previously, Li et al. (37) used a CT-based radiomics signature to predict the HER2 status of gastric cancer, and the radiomics nomogram established by them showed good predictive ability. The AUC of the radiomics signature was 0.799 (95% CI: 0.704-0.894) in training cohort, and 0.771 (95% CI: 0.607-0.934) in validation cohort, respectively. Wang et al. (38) used random forest combined with radiomics to identify the HER2 status of gastric cancer and used arterial phase (AP) and portal phase (PP) CT images for tumor segmentation and feature extraction, respectively. The AUC of the arterial phase radiomics model training cohort was 0.756 (95% CI: 0.656-0.840), and that of the validation cohort was 0.830 (95% CI: 0.678-0.930). The AUC of the portal phase radiomics model training cohort and validation cohort was 0.715 (95% CI: 0.612-0.804) and 0.718 (95% CI: 0.554-0.849), respectively. The above two studies based on radiomics to predict the HER2 status of gastric cancer have shown good prediction and identification performance for the HER2 status of gastric cancer. However, the AUC values of the training and validation cohorts of the two models were lower than the results of this study. Although the two models analyzed the location of the tumor, they did not provide a more detailed description of the tumor that occurred at the special site of the gastroesophageal junction. In this study, the nomogram CT-based radiomics signature combined with CT-reported T stage can better predict the HER2 status of AEG before surgery. It can be used as a non-invasive prediction tool for HER2 status and is expected to guide clinical treatment decisions in clinical practice, and it can assist in the formulation of individualized treatment plans.
The effect of different proportions of toluene and DBP on the preparation of PMMA porous microspheres was emphatically discussed. From Figure 4A to D, the composition of porogen was toluene:DBP = 1:1 (v/v), toluene:DBP = 1:2 (v/v), toluene:DBP = 1:0 (v/v) and toluene:DBP = 0:1(v/v), respectively. It can be seen that the average size of PMMA microspheres after the two-step swelling was about 2.5–3.1 μm. Nevertheless, the surface morphology of the microspheres was different among these four systems. Figure 4A showed that the porous PMMA microspheres had an average pore size and uniform pore distribution, which was smaller than the ones formed by other porogen systems. When the proportion of DBP in the porogen was increased, because of the greater volatility and the water extraction of DBP, phase separation of the microspheres occured during swelling and polymerization, resulting the uneven distribution of DBP within the microspheres (Figure 4B). In Figure 4C, only toluene was used as a porogen. Toluene is poorly water-soluble and has large droplets during the swelling process. Therefore, the resulting porous microspheres have larger pores with the pore size reaching hundreds of nanometers. In Figure 4D, only DBP was used as the porogen. The inside of the microspheres was completely washed out due to the water extraction of DBP, resulting the internal hollow shell structure. From the above analysis, it can be concluded that the porogen system with toluene: DBP of 1:1 (v/v) was the ideal porogen system. Monodispersed porous PMMA microspheres with larger specific surface area can be obtained. Therefore, the microspheres prepared using this porogen system were further tested as a filler for a high-performance liquid chromatography column.
De eerste stap bij het verbeteren van kwaliteit betreft bewustwording en urgentiebesef . De scan KKV draagt hieraan bij doordat hij uitgaat van verschillende perspectieven en deze vervolgens spiegelt aan het kwaliteitskader en de beschikbare feitelijke (beleids)informatie van een locatie. We concluderen dat de elementen van de scan handvatten kunnen bieden voor het verbeteren van de kwaliteit in de verpleeghuissector (en mogelijk ook in andere sectoren). Het gaat hierbij om: 1) het breed ophalen van meningen, 2) het vormen van een integraal beeld van de kwaliteit (dus inhoud en randvoorwaarden), 3) het met elkaar vergelijken van perspectieven, en 4) het uitvoeren van een gedegen analyse door externen, om 5) vervolgens met (vertegenwoordigers van) de diverse betrokkenen gezamenlijk in gesprek te gaan.
Ocimum sanctum L. (O. tenuiflorum) is an important sacred medicinal plant of India known as “holy basil”, Thulasi, Vishnupriya, and Tulsi and worshipped for over more than 3000 years [2, 3]. This herb is popular in traditional medicine as “The Queen of Herbs,” “The Incomparable One,” and “The Mother Medicine of Nature” . Being legendary sacred basil (Tulsi), is recognized [5, 6] not only for its sanctity, but forms an indispensible part of the traditional herbal medicine of East as discussed in Ayurvedic text of Charaka Samhita as well as Unani medicinal system. It is native to India and parts of northern and eastern Africa, Hainan Island, and Taiwan, and grows wild throughout India up to an altitude of 5900 ft (1800 m) in the Himalayas [7–9]. The leaf of the plant owes a stronger, somewhat pungent taste than other basils due to a sesquiterpenoid beta-caryophyllene, and a phenylpropanoid eugenol . O. sanctum has been suggested to possess anti-fertility, anti-cancer, anti-diabetic, anti-fungal, anti-microbial, cardioprotective, analgesic, anti-spasmodic and adaptogenic actions . The chemical composition of Tulsi is highly complex, containing many biologically active phytochemicals with variable proportions among varieties or even plants within the same field. The volatile oil of leaf contains eugenol (1-hydroxy-2-methoxy-4-allylbenzene), euginal, urosolic acid , carvacrol, limatrol, caryophyllene, methyl carvicol while the seed volatile oil has fatty acids and sitosterol. In addition, the seed mucilage contains some levels of sugars and the anthocyans are present in green leaves . The leaf volatiles (terpenes and phenylpropenes) are synthesized and sequestered in glandular hairs present on the leaves, also known as peltate trichomes, which are the characteristic of lamiaceae members [13, 14]. Two types of O. sanctum L. are used for cultivation: (i) plants with green leaves known as Sri/ Rama Tulsi & (ii) plants with purple leaves known as Krishna/ Shyama Tulsi . Furthermore, the quantity of many of its constituents can be significantly altered by varying conditions used for growing; harvesting, processing and storage that are not yet well understood . All of the varieties of Ocimum have unique and individual chemical compositions; but their medicinal properties are not yet explored completely. Despite huge importance of Ocimum, very little transcriptomic and genomic data of Ocimum sp. is available limiting studies on important phytochemical pathways. But comparative transcriptome analysis of Ocimum species (O. sanctum and O. basilicum) was recently reported . This report correlated higher digital expression of phenylpropanoid/ terpenoid pathway genes of O. basilicum to higher essential oil content and chromosome number (O. sanctum, 2n = 16; and O. basilicum, 2n = 48). Also several cytochrome P450s (26) and transcription factor families (40) were identified which could be utilized to characterize genes related to secondary metabolism and its regulation.
3. Non sono soggette all’autorizzazione del costitutore della banca di dati messa per qualsiasi motivo a disposizione del pubblico le attività di estrazione o reimpiego di parti non sostanziali, valutate in termini qualitativi e quantitativi, del contenuto della banca di dati per qualsivoglia fine effettuate dall’utente legittimo. Se l’utente legittimo è autorizzato ad effettuare l’estrazione o il reimpiego solo di una parte della banca di dati, il presente comma si applica unicamente a tale parte.
HF costs are immense, meaning affordability is a problem across various healthcare systems and countries in Europe and around the world [17, 18]. The main driver being an increase in chronic disease and multimorbidity due to an ageing population . As a consequence, healthcare systems face a new challenge; treating multimorbid patients, whilst attempting to keep costs down [20, 21]. HF is not only the fastest growing cardiovascular condition worldwide but also has the highest hospitalisation rates; hence, costs for HF are among the highest for all chronic conditions . Estimates suggest that HF results in 57 billion Euro in direct care costs and 38 billion Euro in indirect costs globally —the equivalent to approximately 2% of total healthcare costs in Europe and North America, over 60% of which are for inpatient care . Of significance, there have been alarming projections of total HF costs increasing by 127% between 2012 and 2030 . The UK’s Centre for Economics and Business Research concluded that the cost of cardiovascular disease in Europe will rise from €100 billion to approximately €120 billion over the next 10 years . Indirect costs of HF also need to be considered, though less explored, as they contribute extensively to the overall burden caused by HF . For example, in Poland, a substantial public finance burden of HF was calculated at €871.9 million in 2012, which increased to €945.3 million in 2015 .
A wide range of model’s performances have been reported; the accuracy values observed by Kansagara et al. ranged from 0.55 to 0.83, with only six models showing a c-index >0.7. In addition, only nine performed external validation in a new set of patients. Artetxe et al. found 64 models reporting performance metrics, ranging from 0.60 to 0.92 (mean value of 0.71). Only 19% showed value of AUC above 0.75. In our reduced final model, the AUC-ROC in the test sample was 0.653, which can be considered at most a modest discrimination ability.
Nos législateurs n´ont jamais vu dans le commerce d´organes une source de greffons mais l´ont toujours condamné et considéré comme un crime, qui porte atteinte à la personne humaine et à sa dignité. Et à ce titre, envisager la pratique de la greffe rénale dans les cliniques chirurgicales privées, ne fait que basculer une partie des patients disposant d´un donneur du secteur public vers celui du prive´, sans pour autant apporter une quelconque solution pour les malheureux patients ne disposant d´aucun donneur, ces cliniques chirurgicales dans les pays qui pratiquent le tourisme de transplantation sont désignées comme étant l´élément clé, facilitateur du commerce d´organes [2, 3]. Pour toutes ces raisons nos législateurs ont conjugué aux dispositions punitives des dispositions préventives, parmi ces dernières il y´a celle n´autorisant cette activité que dans le secteur public .
Al fine di garantire un’adeguata assistenza, i Servizi di Diabetologia hanno attivato procedure di telemedicina e teleconsulto. Questa attività, formalmente riconosciuta e identificata mediante apposito codice di nomenclatore, ha permesso di ricontattare la stragrande maggioranza delle persone già in possesso di appuntamento ambulatoriale, così come ha consentito di creare un punto di contatto con lo specialista di diabetologia sia per la persona con diabete che per il Medico di Medicina Generale. Questo approccio rappresenta, ovviamente, una novità e un passo nel futuro della gestione delle patologie endocrinologiche e metaboliche. Riecheggiando le parole di Trimarchi , d’ora in avanti l’endocrinologo e il diabetologo dovranno imparare a gestire anche a distanza diagnosi e terapie senza perdere di vista aspettative e preoccupazioni delle persone.
Two experiments were conducted to address our research questions, one on-ground and the other in-flight. In both studies, participants performed a self-motion estimation task, presented through virtual reality (VR) goggles (DJI FPV Goggles, https://dji.com/). An IMU recorder was also affixed to the VR goggles to log exact acceleration forces. Within the VR environment, participants were tasked with making a perceptual decision when viewing a drone moving through and out of a cave from a first person perspective. Specifically, participants were asked to predict a collision (or no collision) with either the ceiling or floor of the cave after 1.5 s of motion and just prior to the drone’s egress from the cave. Following their collision response, participants had to provide a confidence estimate on their response on a scale ranging from 0 to 10. The angle of the drone’s trajectory was pseudo-randomly chosen at each trial. Drone trajectory angles ranged from 19.58° downward pitch to 19.58° upward pitch, with a pitch angle difference of 1.96° between trajectories. There were thus 21 trajectories that were rendered in Unity software (https://unity.com/, embedded in PsychoPy (https://www.psychopy.org/ and presented on a Lenovo W541 laptop. Responses were recorded via a Sony Playstation game controller. A total of 420 trials were performed, chunked in 4 blocks. In between blocks, participants were asked to complete a brief questionnaire (NASA TLX,61,62) to index their subjective assessment of task difficulty.Figure 10Schematic representation of a sample trial and stimuli. (a) Trial progression. Participants first view 1.5 s of a video from a first person perspective, where they are moving towards a cave exit. They are then asked to predict whether they would have crashed or not. In the next step, they are asked to provide an estimate of their confidence in their response. (b) Representation of uncertainty induction. Videos were angled at successive intervals from a 0° angle point-of-view (head-on vision). Critical values are angles that, in either up or down directions, lie halfway between a sure, crash-free exit (0°) and a sure crash (highest absolute angle deviating from 0).
In MDCK/FcRn cells, at low and high concentrations of IgG there was significant reduction of viral entry. The two mAbs exhibited significantly different behavior (two-way ANOVA). When compared to ZIKV alone, intermediate concentrations of IgG did not decrease (mAb14) and may have potentially increased (mAb17) viral entry. There were no significant differences in ZIKV entry among various mAb14 concentrations. The enhancement of viral entry in the presence of mAb17 compared to ZIKV alone was not statistically significant; the enhancement was significant compared to 0.3 and 3 ng/mL mAb17. No changes in viral entry were seen at similar (0.4 and 4 ng/mL) concentrations of unrelated IgG.
The electrophoretic mobility of the plasmid DNA pUC18 in the absence and in the presence of HL4 and its metal complexes was studied (Figure 8) to enlighten the features of the interaction between DNA and the compound under study. The differences observed in the migration pattern between the plasmid alone (lane 1) or treated with the vehicle, DMSO (lane 2), and the plasmid incubated with different concentrations of HL4 and L4-M confirmed DNA binding. It is clear that the amount of DMSO used in the experiments does not affect the migration pattern of the plasmid. On the other hand, the experiment with cisplatin (lane 3) which was included as a positive control for covalent binding, showed higher mobility of open circular (OC) and supercoiled (SC) forms than those observed for the free plasmid. In the experiments performed with plasmids treated with L4-Ru (lanes 4–6) and L4-Rh (lanes 7–9), the SC form migrates slower than in the assay with free plasmid and this retardation is dose-dependent. This behavior can be easily related to intercalation processes . A vanishing of the plasmid bands at high drug-DNA concentrations ratio was observed for the L4-Rh/pUC18 system. This may occur due to plasmid saturation with the rhodium complex that prevents ethidium bromide staining, or due to precipitation of the large amount of the rhodium complex bound to DNA. The amount of the OC form is significantly increased in the presence of L4-Ir (lines 10–12), in contrast with its analogues. Thus, the iridium complex efficiently cleaved supercoiled DNA and this cleavage will be further studied. As expected, HL4 shows a different trend in SC migration with respect to its complexes, which reinforces the hypothesis that the metal center plays a key role in the interaction with DNA .
10.7554/eLife.36998.003Figure 1—figure supplement 1.Differential gene expression between E8.5 and E10.5 neuroepithelium.(A) MA plot displaying all expressed genes (FPKM >1) in E8.5 and E10.5 neuroepithelium. Red dots denote differentially expressed genes identified by Cuffdiff. Genes below blue line (y = 0) are enriched in E8.5. (B) Quantitative RT-PCR (qRT-PCR) validation of 75 genes showed positive correlation (R2 = 0.58) with RNAseq data. (C) RNAseq showed upregulation of glial markers, Glast (left) and Blbp (right), in E10.5 neuroepithelium; y-axis shows FPKM values.
In der Gestalt ist diese neue Art ähnlich der Defrancia cordieri Payr. (Kobelt Prodr, p. 143) und deren verwandten Formen, für welche Monterosato die Gattung Cordieria aufstellt, und auch mit einer noch nicht publizierten Art, mit Cordiera hispida Mont. in coll., welche mir aus der Sammlung des Hofmuseums zum Vergleiche vorliegt, zeigt sie vielfach Ähnlichkeit, doch scheint sie schon durch ihre geringen Dimensionen genügend charakterisiert und unterschieden zu sein.
Die chirurgische Therapie erfolgt nach konservativem Therapieversuch und ist immer an die begleitende konservative Therapie gebunden. Die Art der Operation richtet sich hierbei immer nach dem Verlauf, dem Stadium und der Lokalisation des Lymphödems. Generell zeigen sekundäre Lymphödeme ein besseres Ansprechen auf chirurgische Verfahren, als primäre Erscheinungsformen.
Il s’agit d’une étude descriptive menée au centre Mohammed VI pour le traitement des cancers de Casablanca. Nous avons inclus tous les cas de cancer colorectal de manière consécutive nouvellement diagnostiqué et confirmé par un examen histologique durant une période de 12 mois allant du 1er janvier au 31 décembre 2015, et des témoins indemnes de toute maladie cancéreuse (patients parmi les admis au Centre des Consultations de Dermatologie et d’Ophtalmologie de CHU Ibn Rochd Casablanca). Après accord du comité d’éthique local et consentement éclairé signé par le patient et le témoin après avoir reçu l’information nécessaire à sa prise de décision. Les patients et les témoins ont été appariés selon l’âge et le sexe. Le recueil des données s’est fait de manière prospective à partir des dossiers de patients et au moyen d’un questionnaire standardisé englobant plusieurs informations : données sociodémographiques, antécédents personnels et familiaux de cancer, mode de vie, tabagisme, données cliniques et anatomopathologiques (localisation du cancer, histologie, classification TNM, stade et grade de différenciation…), hygiène de vie et habitudes alimentaires. Ce dernier volet qui regroupe la nature, la fréquence, la diversité et la quantité des aliments consommés est adapté au contexte marocain en se basant sur l’étude European Prospective Investigation into Cancer and Nutrition (Epic).
Deși tratamentul de recuperare a fost unul complex, pacientul a fost staționar din punct de vedere funcțional la această internare, evoluția fiind grevată de patologiile coexistente, dar și de o participare selectivă la programul de reabilitare. In ciuda acestui lucru, evoluția globală, de la ultimul accident vascular până în prezent, este favorabilă – pacientul prezintă un grad ridicat de independență. In schimb prognosticul vital rămâne rezervat, existând riscul recidivei accidentului vascular cerebral datorită numeroșilor factori de risc asociați: hipertensiune arterială, diabet zaharat, ateromatoză cervicocerebrală moderată, cardiopatie ischemică, hipetrigliceridemie, accident vascular cerebral vechi în teritoriul arterei cerebrale medii drepte, infarct miocardic anteroseptal sechelar.
Concluzii: Nu avem suficiente date imagistice și clinice legate de existența unor fracturi de stress, frecvent bilaterale apărute în urma unor traumatisme minore sau absența lor, pacienta neprezentând o accentuare a sindrom algic bilateral înainte de ultimul eveniment.
NAIC bestond uit een cisplatinegebaseerd regime (n = 73) of gemcitabine-carboplatin (n = 10). Patiënt- en tumorkarakteristieken – onder andere leeftijd, Charlson Comorbidity Index, ASA-score en nierfunctie – verschilden niet significant tussen de behandelgroepen. NAIC werd bij 11 patiënten (13%) vroegtijdig afgebroken vanwege progressie (n = 6) of toxiciteit (n = 5). ICI werd bij zes patiënten (25%) na twee cycli afgebroken vanwege toxiciteit (p = 0,205). Na NAIC ondergingen patiënten chirurgie (n = 50; 60%) of chemoradiatie (n = 26; 30%) en konden zeven patiënten (10%) geen consoliderende behandeling ondergaan vanwege progressie (n = 5) of toxiciteit (n = 2). Na ICI ondergingen alle patiënten chirurgie. Na chirurgie (n = 74) werd pCR door respectievelijk 11 (22%) NAIC- en 11 (48%) ICI-patiënten bereikt (n = 0,056). pCD werd door respectievelijk 17 (35%) NAIC- en 14 (58%) ICI-patiënten bereikt (p = 0,077). Patiënten die waren behandeld met NAIC kregen vaker progressie (43% vs. 8%; p = 0,001). Mediane (IQR) follow-up was 26 (20-32) maanden. In het gehele cohort (n = 107) was ICI geassocieerd met betere PFS (p = 0,003) en overleving (p = 0,003). Zie figuur 17.1.
Intercellular epithelial‐fibroblast cell communication in HGSOC. (A, B) Detailed view of ligand‐receptor connections between each epithelial cell subcluster and five fibroblast cell subclusters. Each color arrowline indicates the ligands to receptors from one cell population to another. The arrowline thickness is proportional to the number of ligand‐receptor pairs. (C, E) Overview of selected interactions of receptors expressed by epithelial cells and ligands expressed by fibroblast cells. (D, F) Overview of selected interactions of receptors expressed by fibroblast cells and ligands expressed by epithelial cells
In the present study, using a transcription array to identify transcription factors activated by LPA-mediated signaling, we demonstrate that LPA potently stimulates the activation of HIF1α via a pathway involving Gαi2 and Src. We further demonstrate that that the activation of LPA-Gαi2-Src-mediated signaling pathway induces EMT in ovarian cancer cells and subsequent invasive migration of ovarian cancer cells that can be inhibited by PX-478, a clinically tested inhibitor of HIF1α. Thus, our current study demonstrates that LPA stimulates a signaling nexus involving Gαi2, Src, and HIF1α to induce EMT and migration of ovarian cancer cells. Furthermore, we show that Gαi2 signaling is necessary and sufficient for hypoxia-mediated induction of HIF1α expression, which has not been shown, to our knowledge, by any previous studies to date.
A—Hydroxyurea (HU) produces a concentration-dependent inhibition of worm growth over a 7 day period in RPMI+50% CS; B—7 days HU (25 mM) exposure slows the rate of growth in juveniles maintained in RPMI+50% CS, following HU removal growth rate increases during a subsequent 3 day recovery period. Numbers beside points refer to figures below; C—Increase in worm size following removal of HU (1 vs 3) suggests recovery of growth; D—EdU accumulation does not recover significantly following HU removal. E—EdU labelled nuclei in juveniles maintained in RPMI+50% CS and 25 mM HU over 7 days as seen in ‘Treatment 1’ in B; F—EdU labelled nuclei in juveniles maintained in RPMI+50% CS over 7 days as seen in ‘Treatment 2’ in B; G—EdU labelled nuclei in juveniles maintained in RPMI+50% CS and 25 mM HU for 7 days before HU is removed as seen in ‘Treatment 3’ in B; H—EdU labelled nuclei in juveniles maintained in RPMI+50% CS over 10 days as seen in ‘Treatment 4’ in B; in all graphs mean +/-SEM is presented (shown in red on scatter graphs); in scatter graphs each data-point represents a measurement from an individual worm. Statistical analyses were performed using One Way ANOVA with Dunnett’s post hoc test against untreated “0” sample (A), t-test on days 3, 7 and 10 (B) or Kruskal-Wallis test with Dunn’s post hoc test to compare medians with all other medians (C, D). , p<0.01; , p<0.001; ***, p<0.0001.
Le diagnostic de dermatomyosite a été porté chez notre patiente devant l'association des 5 critères diagnostiques de Bohan et Peter . La panniculite a été diagnostiquée environ 4 ans après l'apparition des premières manifestations en rapport avec la DM. L'association d'une panniculite symptomatique et d'une DM est très rare. En effet, depuis sa première description en 1924 par weber et Gray , cette association n'a été rapportée à notre connaissance que dans 18 cas [6–21]. Dans la majorité des cas, la panniculite était de survenue concomitante avec les premières manifestations de la DM ou au cours des poussées de la maladie contrairement à notre observation puisque la panniculite était découverte en phase de rémission complète clinique et biologique de la DM. Il s'agissait de femmes âgées entre 50 et 65 ans dans la plupart des cas rapportés, à part quelques observations pédiatriques [9, 18, 20]. L’évolution était le plus souvent favorable après traitement par des corticoïdes associés dans certains cas à l'azathioprine ou la ciclosporine [16, 17]. Aucun de ces patients n’était traité par du MTX. Le MTX était incriminé dans la survenue ou l'aggravation de nodules rhumatoïdes dans 8 à 10% des cas de polyarthrite rhumatoïde . Par contre, l'apparition d'une panniculite au cours du traitement par du MTX est une situation très rare qui n'a été décrite à notre connaissance que dans 3 cas seulement [1–3]. Il s'agissait d'une panniculite au cours du traitement par du MTX d'un rhumatisme psoriasique , d'une dermatomyosite et d'un syndrome de Sharp . Les mécanismes physiopathologiques de la panniculite iatrogène au MTX restent indéterminés. Dans le premier cas de Berris la survenue des nodules mettait en cause le diagnostique de rhumatisme psoriasique en faveur d'une polyarthrite rhumatoïde où il était « habituel » d'observer une apparition ou une aggravation des nodules rhumatoïdes chez les patients traités par du MTX. Cependant, les lésions cutanées du psoriasis étaient évidentes, le facteur rhumatoïde était négatif et l'examen anatomopathologique d'un nodule cutané objectivait une panniculite septale. En 1999, Jang rapportait une panniculite chez une patiente de 50 ans atteinte d'une dermatomyosite et traitée par du MTX. Les nodules cutanés étaient apparus 14 mois après le début du traitement par le MTX, initialement situés au niveau du décolleté, puis rapidement une extension vers le cou, les creux axillaires et les membres supérieurs a été observée. L'examen anatomopathologique objectivait une panniculite septale. Les nodules ont totalement disparu après l'arrêt du MTX et le traitement par de l'hydroxychloroquine. La panniculite observée chez notre patiente peut être en rapport avec la DM ou secondaire au traitement par le MTX. Cette deuxième éventualité semble la plus probable. En effet, les lésions cutanées de la panniculite sont apparues après l'introduction du MTX et à un moment où la DM était quiescente. D'autre part, la persistance de la panniculite après l'augmentation des doses de corticoïde et sa disparition à l'arrêt du MTX sont des arguments chronologiques et évolutifs qui plaident en faveur de l'origine iatrogène médicamenteuse.
The migratory and invasive capabilities of GC cells were determined using transwell assay (pore size, 8 μm; Corning Incorporated, NY, USA). For the transwell invasion assay, the upper surface of the transwell chambers were precoated with Matrigel (BD Biosciences, Sparks, USA). GC cells were cultured in serum-free RPMI-1640 overnight and were added into the upper chambers (3 × 104 cells for the migration assay and 5 × 104 cells for the invasion assay). For both the migration and invasion assays, the medium in the upper chamber was filled with serum-free RPMI-1640, and the lower chamber was filled with RPMI-1640 medium containing 10% FBS. After incubation at 37 °C for 24 h, cells on the upper surface of the membranes were removed with a cotton swab, and cells on the lower surface of the membranes were fixed with 4% paraformaldehyde for 30 min and stained with crystal violet solution (Beyotime, Shanghai, China) for 10 min. The number of migrated or invaded cells was counted under a microscope from five random fields. All experiments were performed in triplicate.
Last, to test the mediating role of risk perception, bootstrapping was employed. Regression analysis was performed using PROCESS Model 4 developed by Hayes . Specifically, three regression equations were run to verify the following conditions: (a) independent variable (X) affects dependent variable (Y); (b) independent variable (X) affects mediator (M); and (c) mediator (M) affects dependent variable (Y) . Fully mediating associations were tested when the direct effect of X on Y was not statistically significant after controlling for the effect of M; partial mediation was tested when controlling for M, and the direct effect of X on Y was significant . A bootstrapping procedure with n = 5000 bootstrap re-samples was used to evaluate the indirect effects . An indirect effect was tested significant if the 95% bias corrected and accelerated bootstrapped confidence interval (Boot95%CI) excluded zero.
After passing through the bipolar charger, the aerosol flows to the DMA. As shown in Fig. 1, the DMA is a long cylindrical chamber with a central rod concentric to the walls of the chamber so that an annulus is formed between the rod and the chamber walls. The rod voltage can be adjusted from about −10 V to −10 000 V. The outer cylindrical chamber is kept at ground voltage, allowing for an electrical field to develop within the annulus. The aerosol flow enters the top of the chamber and is joined by a sheath flow of clean air. Both flows travel through the annulus to the bottom of the chamber. Along the way, positively charged particles move toward the center rod due to the rod voltage. A small slit in the rod allows for the passage of particles with electrical mobility with an approximate mean mobility, Z(x = 1), computed by Eq.(15). The flow leaving the DMA, now comprised of nearly monosize particles, passes through the rod opening, exits the classifier, and then flows into the condensation particle counter where the number of particles is counted. The rest of the flow leaves the classifier through an excess flow outlet and enters the recirculation system.
Les protéines de haute qualité biologique ne devraient pas manquer dans l’alimentation quotidienne des personnes infectées par le VIH [8, 9, 20, 21] et les protéines végétales devraient être préférées à celles animales. En plus de la viande, du poisson et des produits laitiers, il existe pour un approvisionnement adapté en protéines des combinaisons peu coûteuses d’aliments végétaux ayant une qualité biologique élevée (par exemple ragout de haricots rouges avec sauce d’arachides et graines de mais) ainsi que le soja, qui en plus de flavonoïdes est riche en acides gras insaturés. Il est recommandé de manger du poisson riche en acides gras Omega-3 et Omega-6 comme par exemple le hareng, le maquereau ou le thon, car ces acides gras jouent un rôle important dans le renforcement du système immunitaire .
The left task is to specify the expressions of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\dot{W}}_{p}$$\end{document}W˙p and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\dot{W}}_{y}$$\end{document}W˙y. In the Spporting Online Material of this paper, we have showed11\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\dot{W}}_{p}=\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}-{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}{E}\,\mathrm{ln}\,\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}}{{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}\equiv J\,\mathrm{ln}\,q$$\end{document}W˙p=λ12λ23λ34λ41−λ43λ32λ21λ14Elnλ12λ23λ34λ41λ43λ32λ21λ14≡Jlnqwhich is just the energy dissipation of a circulation per unit time (see refs38,52,53), where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$J=\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}-{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}{E}$$\end{document}J=λ12λ23λ34λ41−λ43λ32λ21λ14E and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$q=\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}}{{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}$$\end{document}q=λ12λ23λ34λ41λ43λ32λ21λ14. Regarding to the calculation formula for \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\dot{W}}_{y}$$\end{document}W˙y, we can show (seeing the Spporting Online Material for details)12\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{rcl}\dot{W} & = & (\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}-{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}{E})\,\mathrm{ln}\,\frac{{\lambda }_{12}{\lambda }_{23}{\lambda }_{34}{\lambda }_{41}}{{\lambda }_{43}{\lambda }_{32}{\lambda }_{21}{\lambda }_{14}}\\ & & +\frac{2}{{\rm{\Phi }}}[\frac{2A+2B}{E}+\frac{{\delta }^{2}(C{\sigma }_{2}^{2}+D{\sigma }_{1}^{2})}{E}]+\frac{2\delta (A+B-C-D)}{E}\\ & & +\frac{{\rm{\Phi }}}{2E}[(A+B){\delta }^{2}+\frac{C}{{\sigma }_{2}^{2}}+\frac{D}{{\sigma }_{1}^{2}}]\end{array}$$\end{document}W˙=(λ12λ23λ34λ41−λ43λ32λ21λ14E)lnλ12λ23λ34λ41λ43λ32λ21λ14+2Φ[2A+2BE+δ2(Cσ22+Dσ12)E]+2δ(A+B−C−D)E+Φ2E[(A+B)δ2+Cσ22+Dσ12]
Признак «тетраостость» пшеницы изучен в меньшей степени. Н.И. Вавилов и О.В. Якушкина (1925) исследо- вали характер наследования ряда признаков, характерных для «Персидской пшеницы» T. carthlicum, среди которых был признак «длина остевидных придатков колосковых чешуй». Они провели масштабный гибридологический анализ, скрещивая T. carthlicum с различными видами ди-, тетра- и гексаплодных пшениц, а также эгилопсами и рожью (всего 64 комбинации скрещиваний), и изучили закономерности наследования признаков. Была отмечена сложная генетическая природа признака, показано, что он контролируется несколькими генами. В других работах был обнаружен рецессивный характер наследования при- знака тетраостости у T. carthlicum (Мигушова, Жуковский, 1969). По данным П.А. Гандиляна (1973), наличие тетраостости (персикоидности) связано со специфическим геном Т. Р.В. Рожков с коллегами (2014) показали, что признак «тетраостость» T. carthlicum и T. petropavlovskyi при скрещивании с сортами твердой (T. durum) и мягкой (T. aestivum) пшениц соответственно, наследуется как ре- цессивный. При этом, если при скрещивании T. petropavlovskyi с мягкой пшеницей за формирование тетраостости отвечает один ген, то в комбинациях с T. persicum влияние оказывают несколько генов.
Previous studies analysed ride-hailing user characteristics from household travel surveys or designated surveys distributed among service users (e.g. [1–5]). These studies concur that better educated and wealthy travellers aged 20–39 make up the predominant user group of ride-hailing services. In contrast, their findings differ in regard to the impact of ride-hailing on PT trips. Specific trip attributes of the ride-hailing option as well as the mobility alternatives are expected to be more relevant for explaining the potential substitution of ride-hailing than socio-demographic characteristics of the individual . An analysis of Uber data from 24 US regions concluded that areas with diverse land-uses, dense population, employment and PT services are characterized by high Uber demand as well as high overall travel demand . In contrast, Uber demand is, all else being the same, lower in areas having better accessibility by private car and PT, as those are associated with a higher competitiveness of alternative travel modes. PT, especially urban rail, was positively associated with adopting ride-hailing services in large metropolitan areas in the US [7, 8]. Another analysis based on 50 US agency-level data from 2000–2017 concluded that ride-hailing are neither a complement nor a substitute of bus services . Interestingly, studies that controlled for individual attitudes related to where one chooses to live and perceptions of public transport found that the substitution relation between ride-hailing and public transport becomes insignificant . This illuminates the need to examine the impact of ride-hailing on accessibility in different urban contexts which are characterized by varying urban rail development levels.
Si les infections à Streptococcus sp et Staphylococcus sp sont les plus fréquentes, l′implication d’autres agents pathogènes (agents fongiques, Bacilles Gram Négatif, germes anaérobies) est rarement rapportée dans la littérature, et souvent en rapport avec une immunodépression.
For TEM imaging, the following procedure was used; in 1 ml 2.5% glutaraldehyde at pH 7, the exosomal pellet was dissolved and redissolved in 0.1 M sodium cacodylate solution for one hour. Following removal of the fixative, the pellet was washed three times in 1 mL 0.1 M sodium cacodylate buffer at 25 °C for 10 min, each, followed by incubation in 1 mL of 2% osmium tetroxide for 60 min at 4 °C. Once again, the fixative was removed and the pellet was washed in 0.1 M sodium cacodylate buffer three times, 10 min each. The samples were subsequently dried with graded acetone series. Acetone was added to low-viscosity embedding medium series (3:1, 1:1, 1:3). Afterward, samples were incubated with low-viscosity embedding medium (100%). The samples were embedded using an embedding mold at 4 °C and baked for one day. The embedded samples were then cut into 60 nm thick sections using an ultra-microtome. Thereafter, the sections were stained with 2% uranyl acetate and lead citrate for 20 and 10 min, respectively. Finally, the samples were observed under the grid using JEOL® (Peabody, MA, USA) TEM model JEM-1200EX at 80 kV.
Het overgrote deel van de type 2-diabetespatiënten in Nederland wordt behandeld in de eerste lijn, waarbij de diëtist een prominente rol speelt. 47% van de diëtisten geeft aan dat diabetespatiënten hun grootste patiëntengroep vormen, blijkt uit onderzoek van de Nederlandse Vereniging van Diëtisten (NVD).1 Minstens de helft van de diabetespatiënten krijgt uiteindelijk te maken met bijkomende complicaties of comorbiditeiten.2,3 Dit percentage neemt toe naarmate de diabetespatiënt ouder wordt.4 Meer dan driekwart van de diëtisten in het eerdergenoemde NVD-onderzoek geeft aan diabetespatiënten met één of meer comorbiditeiten in hun praktijk te zien. De meest voorkomende diabetesgerelateerde comorbiditeit is diabetische nefropathie of een andere vorm van nierschade.5,9 In 2020 zijn de voedingsrichtlijnen voor diabetespatiënten met nierschade herzien. Dit maakt dat de eerstelijns diëtist meer verantwoordelijkheid heeft gekregen voor deze patiënten. Niet alleen is uit onderzoek gebleken dat het voor deze groep erg moeilijk is om een gepersonaliseerd dieet samen te stellen, van een diëtist wordt nu verwacht dat ze zelfstandiger stappen kan ondernemen. De richtlijnen onderstrepen dat samenwerking tussen diëtist en arts belangrijker is geworden. Hierbij is het doel om de diabetespatiënt met nierschade een betere kwaliteit van leven te geven.
The samples were washed several times in PBS prior to fluorescent detection on the ModulusTM Microplate Multimode Reader (Turner Biosystems, Promega, Southampton, UK) using the blue filter (excitation 490 nm, emission 510-570 nm), which had spectra closest to that of the NPs.
Zespół łamliwego chromosomu X jest jedną z głównych genetycznych przyczyn wystąpienia niepełnosprawności intelektualnej (NI) i autyzmu, zaś badanie molekularne w kierunku identyfikacji mutacji dynamicznej w genie FMR1 jest zlecane jako jedno z pierwszych badań diagnostycznych w przypadkach stwierdzenia NI, opóźnienia rozwoju psychoruchowego czy zaburzeń zachowania. Potwierdzenie rozpoznania klinicznego FXS jest podstawą do wdrożenia odpowiedniego postępowania terapeutycznego, które w przyszłości wraz z rozwojem nowych sposobów leczenia może mieć charakter spersonalizowany. Identyfikacja mutacji dynamicznej u probanta jest podstawą do objęcia pacjenta i jego rodziny opieką przez poradnię genetyczną. Osoby z premutacją w genie FMR1 – nosiciele zespołu łamliwego chromosomu X, są potencjalnymi pacjentami poradni ginekologicznej i poradni neurologicznej, ze względu na ryzyko wystąpienia u nich zespołu przedwczesnego wygasania czynności jajników oraz zespołu drżenia i ataksji związanego z zespołem FXS (odpowiednio, FXPOI – Fragile X-associated primary ovarian insufficiency i FXTAS – Fragile X-associated tremor/ ataxia syndrome). Stąd też istotne jest wdrożenie jak najbardziej optymalnych procedur diagnostycznych, które w sposób wiarygodny potwierdzą bądź wykluczą rozpoznanie zespołu łamliwego chromosomu X i innych chorób FMR1-zależnych.
El actual método de elección para disminuir el tiempo y el costo del cribado in vitro es el análisis in silico (virtual screening), lo que permite filtrar los mejores compuestos para, posteriormente, ser probados in vitro. Los candidatos más prometedores se someten a modificaciones químicas para mejorar parámetros como la toxicidad y la estabilidad, ser probados en modelos animales y, por último, ser sometidos a estudios clínicos.
These three candidates have been selected to show the ability of our new technique to detect alternative prenylation modes. RasD2 (synonym: Rhes, BC013419) is suggested to be a farnesylation target but only due to indirect evidence . Whereas K-Ras homologues such as v-Ki-Ras2 (the Q61H oncogene mutant of K-Ras4B, BC013572) are thought to be modified both by FTase and GGTase1 , the RhoA protein (NM_001664.2) is primarily a GGTase1 target .
The homo-bifunctional membrane-impermeant cross-linker DTSSP has previously been used to show the trimeric assembly of P2X receptors; however, its site(s) of action were not determined. DTSSP reaction with the P2X1 receptor results in a thioacyl or thioacyl iodo modification of the receptor that can be detected by mass spectrometry. Of the 19 extracellular lysine residues, 10 were observed to be modified by DTSSP (K127, K136, K138, K140, K199, K215, K221, K283, K309 and K322) and residue K68 was only detected in the native state in 11/11 runs indicating that this residue does not bind DTSSP. Peptides corresponding to the remaining eight lysine residues were only observed in one to four of the 11 runs and only the native un-modified lysine was detected; thus, for these eight residues, it remains unknown whether they are modified (Fig. 3; Table). Surprisingly DTSSP modification was also observed at residues S130, S286 and Y274, similar labelling of serine and tyrosine residues by DTSSP has been reported previously (Swaim et al. 2004). Mapping the sites of DTSSP modification onto a human P2X1 receptor homology model (Allsopp et al. 2011) shows that these residues are all in accessible positions on the surface of the receptor (Fig 3.) and provides independent support for the validity of the P2X1 receptor homology model.
Combining all, we would like to say that the aqueous extract of TA protects liver and kidney tissues against oxidative damages and could be used as an effective protector against CCl4 induced hepatic and renal damages. Further works are needed to fully characterize the responsible active principle(s) present in the plant and elucidate its possible mode of action and that is in progress.
The comparison of the signal decomposition techniques has been mainly performed on a well-known experimental benchmark, which was proposed by the Los Alamos National Laboratory (LANL) and fully described in References . The main points are here recalled for completeness. The test structure is a three-storey shear-type frame, excited horizontally at the moving base. A scheme of the test frame, adapted by the authors from Ref. , is reported in Figure 5 (the shaker and the sensors’ positions are highlighted). Please note that only one output channel per floor was available. However, this is sufficient to fully characterise the model under the assumption of shear-type frame behaviour, which is well justified here. The model presents two typical forms of detectable damage-induced features: (1) a localised reduction of the stiffness, and (2) a pointwise source of nonlinearity. It also introduces added masses to simulate damage-unrelated operational changes .
Dokładny wywiad i badanie przedmiotowe wraz z uwzględnieniem objawów alarmowych oraz odpowiednio dobrane badania dodatkowe, stanowią kluczowe narzędzia w identyfikacji pacjentów z chorobą organiczną. W diagnostyce różnicowej należy również uwzględnić rzadkie przyczyny przewlekłych bólów brzucha, w tym możliwość wystąpienia choroby onkologicznej. Obraz kliniczny guzów neuroblastyczych jest niezwykle różnorodny, a lekarze powinni być świadomi nie tylko klasycznych objawów choroby, ale również tych nietypowych. Ustalenie wskazań do poszerzenia diagnostyki pozwala na szybkie postawienie rozpoznania i wdrożenie odpowiedniego postępowania terapeutycznego, co przekłada się na poprawę wskaźników przeżycia i zminimalizowanie nieodwracalnych uszkodzeń.
SET-NUP214融合基因由染色体平衡易位t（9;9）（q34;q34）或9号染色体长臂3区4带隐匿性缺失del（9）（q34.11q34.13）形成，首次报道于一例急性未分化白血病患者。SET-NUP214融合基因主要见于急性T淋巴细胞白血病（T-ALL），在T-ALL中发生率为3.0％～10.3％，在急性髓系白血病（AML）和急性B淋巴细胞白血病（B-ALL）中亦有报道–。目前认为具有SET-NUP214基因重排的血液恶性肿瘤患者预后较差，由于文献报道较少，对于此类疾病的特征尚缺乏较为一致的认识。本研究通过总结我院收治的24例SET-NUP214融合基因阳性血液恶性肿瘤患者的临床资料，以期初步探讨SET-NUP214基因重排在恶性血液病中的分布情况及其相关的临床生物学特性，提高对此类疾病的认识水平。
The experimental literature on pragmatic language use is enormously complex. As noted earlier, many studies offer conflicting findings in regard to how people pragmatically produce and interpret various aspects of communicative meaning. These profound variations in experimental outcomes relate to a broader concern within psychology and elsewhere, dubbed as the “replication crisis.” Failures to replicate are now being published more than ever with some scholars claiming that any variation from some empirical standard should be interpreted as casting doubt on the validity of some earlier obtained experimental result (both for exact and conceptual replications) (Shrout and Rodgers, 2019).
В поперечное ретроспективное исследование был включен 391 пациент, перенесший аллотрансплантацию трупной почки в ГБУЗ «МОНИКИ им. М.Ф. Владимирского» в период с 2007 по 2019 г. Из них в анализ были включены данные 236 пациентов (114 мужчин, 122 женщин) со сроком наблюдения после АТП более 3 мес, для которых были доступны результаты анализов крови на интактный паратиреоидный гормон (ПТГ), общий кальций, фосфор, щелочную фосфатазу (ЩФ) и креатинин, измеренные одномоментно в рамках одного визита. Проведение исследования одобрено Независимым комитетом по этике при ГБУЗ МО «МОНИКИ им. М.Ф. Владимирского» без подписания информированного согласия для участников ввиду его неинтервенционного характера (Протокол № 12 от 08.07.2021). Все процедуры были проведены в соответствии с Хельсинкской и Стамбульской декларациями.
HIV-1 protease and reverse transcriptase (RT) sequences were obtained using standard population sequencing adapted from previously described methods. HIVDR testing was performed at the National Health Services Laboratory, University of Witwatersrand, Johannesburg, South Africa, a WHO-designated HIVDR genotyping laboratory. RECall was used to generate consensus sequences; quality assurance was performed following WHO recommendations. The Stanford HIV drug resistance algorithm (HIVdb), version 8.4, was used to identify HIVDR resistance profiles, mutations, and HIV subtypes; sequences classified as having low-, intermediate-, or high-level HIVDR were considered as resistant. The HIV-1 integrase region was not sequenced because at the time of this study integrase strand transfer inhibitors (InSTI) were not prescribed for treatment of HIV in Namibia's public health sector.
Closer examination of the ∆f vs. ∆Γ diagrams gives an interesting insight into the behaviour of the coagulation process on the QCM-D. The gradients change throughout the measurement (compare red dotted lines (slopes) at stages ①, ② and ③ in Figure 2a,b. This explains to a high degree the deviation of the overall linear fit. On an uncoated quartz (Figure 2a) the curve begins with a very steep slope (Figure 2a(①)), representing massive adsorption which gets less steep over time (Figure 2a(②,③)) as the surface saturates. The initial massive adsorption on uncoated quartzes is undesired since it handicaps the distinction between blank and coagulation samples when it comes to quantitative measurements of the fibrinogen levels. On a coated quartz (Figure 2b) the slope kinetic is inverse to an uncoated quartz (Figure 2a) with virtually no mass effect in the beginning (Figure 2b(①)) and mostly changes in viscosity. Interestingly, over time the mass effect increases (Figure 2b(②,③)) due the coupling of the clot and the fact that the fibrin net renders the viscoelastic coating more rigid.
Nach Beginn der Corona-Pandemie Ende 2019 in China und danach im Rest der Welt, trafen mit nur wenigen Wochen Verzögerung die ersten Berichte über das Auftreten von Riechstörungen im Rahmen der Infektion mit SARS-CoV‑2 ein. Ein deutlicher Anteil (ca. 30–65 %) der symptomatischen Patienten berichteten über eine plötzlich einsetzende Anosmie, die neben den typischen Symptomen der COVID-19-Erkrankung, wie Fieber, Husten, Atemnot, Halsschmerzen, auch als alleiniges Symptom auftrat . Folglich wurde der Verlust des Riechvermögens im Frühjahr 2020 von den medizinischen Fachgesellschaften und Behörden als hinweisendes Warnsignal auf das Vorliegen einer COVID-19-Erkrankung definiert . Die Dauer der Riechstörung wurde anfangs als reversibel und kurz beschrieben, bald wurde jedoch klar, dass ein Teil der betroffenen Patienten auch noch nach längerer Zeit an dem Verlust des Geruchssinns leiden. So konnte auch unsere Forschungsgruppe bereits im Frühjahr 2020 über hundert Betroffene mit persistierendem Riechverlust in eine Langzeit-Beobachtungsstudie einschließen. Erst in den nächsten Jahren ist zu erwarten, die Langzeitprognose aufzuklären.
Vom 3. HWK mit dem Übergangssegment C3/C4 (C4) bis zu Th1 bzw. dem Segment C7/Th1 (C8) sprechen wir von der unteren HWS, die durch relativ vergleichbar aufgebaute Halswirbelkörper gebildet wird. Auch deren Bewegungsspektrum ist in allen drei Dimensionen ähnlich (Tab. 1). Es gilt für die HWS ab C2/3 eine gesetzmäßige Bewegungskoppelung (Fryett-Regel), die besagt, dass sich Rotation und Seitneigung der Halswirbelkörper gleichsinnig bewegen. Bei einer Seitneigung nach rechts rotieren die HWK ebenfalls nach rechts, besonders gut an der Abweichung der Dornfortsätze nach links palpabel. Diese Koppelung kann in der manuellen Medizin durch Rotation und kontralaterale Seitneigung (sogenannte 15°/15°-Regel) und der damit erreichten funktionellen Neutralstellung der HWS zum Schutz der A. vertebralis bei bestimmten manipulativen und auch mobilisierenden Techniken eingesetzt werden (; Abb. 4).
The validation of the linearization approach for the generic class AB PA of Section 2.3 was carried out over the aforementioned test bench (Figure 5). As in the previous section, the probe signal was set following a 5G-NR format characterized by a total bandwidth of 30 MHz and a PAPR of 10.5 dB, and the operating point to obtain the model structure was set to an output power level of 27.6 dBm. The attained structure was used for the validation in a signal generator power sweep from −34 to −17 dBm with 1 dB of step size. The output was acquired with a sampling rate of 92.16 MHz, which implies an oversampling factor of 3. This sweep corresponds to an output power range from 21 to 34 dBm and a gain compression varying from 0.6 to 6.0 dB.
Course of parasitemia during self-healing infections of P. chabaudi in vaccination-protected Balb/c mice and lethal infections in non-vaccinated mice. Values represent means determined from three mice whose livers were taken to analyze miRNA expression. Bars represent half SD and star indicates significant difference (p < 0.01).
Las limitaciones del estudio son propias de las fuentes de datos disponibles para un análisis en escala de municipio, como son los censos poblacionales y de viviendas que, en algunos países tienen más de 15 años, o por no disponibilidad de datos de calidad o de variables ambientales y socioeconómicas, como por ejemplo de renta y diferenciación entre áreas urbanas y rurales. Otra limitación es que la escala de municipio es diferente a la escala operacional de foco de LC y la identificación de determinantes de brotes epidémicos a dicha escala, que son puntuales en tiempo y espacio. Por otra parte, en relación con la vigilancia y registro de casos de LC, el sitio de notificación puede ser diferente al sitio de transmisión (por ejemplo, casos que ocurren en soldados, migrantes forzados o migrantes estacionales), lo que requiere un sistema de comunicación fluida entre los sitios de producción de casos y de recepción y diagnóstico. También hay limitaciones y posibles sesgos debidos al subregistro por falta de accesibilidad y sensibilidad diagnóstica del sistema de salud, lo que potencia la vulnerabilidad de las poblaciones ya vulnerables.
To validate the various functions of the meta-axicon in its different configurations, we measured the patterns that were transmitted along the light propagation direction. Figure 3 shows a schematic diagram of the experimental setup. In the experiments, a wavelength of 780 nm is used. The laser light is converted into CP light using the quarter-wave plate (QWP1) that is located in front of the sample. A component with a specific circular polarization is selected using a linear polarizer (LP) and a quarter-wave plate (QWP2), and the imaged patterns are then recorded using a charge-coupled device (CCD) camera.Figure 3Schematic diagram of the experimental setup. A: attenuator; QWP: achromatic quarter-wave plate; LP: linear polarizer; CCD: charge-coupled device.
The camera’s optical system introduces certain deformations called distortions that cause displacement of point (x,y) within the background plane into a different position (x′,y′). The new position, taking into account radial and decentring distortion, can be expressed as:(3)x′=x(1+K1r2+K2r4+K3r6+K4r8)+(P1(r2+2x2)+2P2xy), (4)y′=y(1+K1r2+K2r4+K3r6+K4r8)+(P2(r2+2y2)+2P1xy), where: K1, K2, K3, K4 are radial symmetric distortion coefficients, P1, P2 are decentring distortion coefficients (including both radial asymmetric and tangential distortions), while r is the radial radius, defined as: (5)r=x2+y2.

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