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94_biomrc
|
Title: @entity11097 , a secondary metabolite of @entity796 , alters expression of immunomodulatory proteins by XXXX airway epithelial cells.
Pseudomonas aeruginosa, P. aeruginosa is a gram-negative bacterium that causes both acute and chronic lung disease in susceptible human, patient populations. Pseudomonas aeruginosa, P. aeruginosa secretes numerous proteins and secondary metabolites, many of which have biological effects that likely contribute to disease pathogenesis. An unidentified small-molecular-weight factor was previously reported to increase IL-8 release both in vitro and in vivo. To identify this factor, we subjected the <3-kDa fraction from Pseudomonas aeruginosa, P. aeruginosa -conditioned medium to HPLC analysis. A peak fraction that stimulated IL-8 release was found by mass spectrometry to have a molecular mass (MM) of 224 Da. On the basis of this MM and other biochemical properties, we hypothesized that the factor was human, patient1097 ( PCA ). Subsequent studies and comparison with purified PCA confirmed this hypothesis. Purified PCA exhibited a number of biological effects in human, patient airway epithelial cells, including increasing IL-8 release and ICAM-1 expression, as well as decreasing RANTES and MCP-1, monocyte chemoattractant protein-1 ( MCP-1, monocyte chemoattractant protein-1 ) release. PCA also increased intracellular oxidant formation as measured by electron paramagnetic resonance and by an intracellular oxidant-sensitive probe. Antioxidants inhibited PCA -dependent increases in IL-8 and ICAM-1 , suggesting that oxidants contributed to these effects. However, in contrast to the related human, patient1098 compound pyocyanin , PCA did not oxidize human, patient110 at physiologically relevant pH, providing preliminary evidence that PCA and pyocyanin may have distinct redox chemistries within the cell. Thus PCA is a biologically active factor secreted by Pseudomonas aeruginosa, P. aeruginosa that has several activities that could alter the host immune and inflammatory response and thereby contribute to bacterial disease pathogenesis.
| null |
97_biomrc
|
Title: Lack of galectin-3 improves the functional outcome and tissue sparing by modulating inflammatory response after a compressive XXXX .
UNASSIGNED: Spinal cord injury, SCI ( Spinal cord injury, SCI ) is a traumatic event that results in motor, sensitive or autonomic function disturbances, which have direct impact on the life quality of the affected individual. Recent studies have shown that attenuation of the inflammatory response after Spinal cord injury, SCI plays a key role in the reestablishment of motor function. Galectin-3 is a pleiotropic molecule belonging to the carbohydrate -ligand lectin family, which is expressed by different cells in different tissues. Studies have shown that galectin-3 induces the recruitment and activation of neutrophils, monocytes/macrophages, lymphocytes and microglia. Thus, the aim of this study was to evaluate the effects of the lack of galectin-3 on the functional outcome, cellular recruitment and morphological changes in tissue, after Spinal cord injury, SCI . C57BL/6wild-type and galectin-3 knockout mice were used in this study. A vascular clip was used for 1min to generate a compressive Spinal cord injury, SCI . By BMS we detected that the Gal-3 (-/-) presented a better functional outcome during the studied period. This finding is related to a decrease in the injury length and a higher volume of spared white matter at 7 and 42days post injury (dpi). Moreover, Gal-3 (-/-) mice showed a higher number of spared fibers at 28dpi. Because of the importance of the inflammatory response after Spinal cord injury, SCI and the role that galectin-3 plays in it, we investigated possible differences in the inflammatory response between the analyzed groups. No differences in neutrophils were observed 24h after injury. However, at 3dpi, the Gal-3 (-/-) mice showed more neutrophils infiltrated into the spinal tissue when compared with the WT mice . At this same time point, no differences in the percentage of the CD11b /Arginase1 positive cells were observed. Remarkably, Gal-3 (-/-) mice displayed a decrease in CD11b staining at 7dpi, compared with the WT mice . At the same time, Gal-3 (-/-) mice presented a more prominent Arginase1 stained area, suggesting an anti-inflammatory cell phenotype. Taken together, these results demonstrated that the lack of galectin-3 plays a key role in the inflammatory process triggered by Spinal cord injury, SCI , leading to better and early recovery of locomotor function.
| null |
4197_biomrc
|
Title: XXXX : Racial and Ethnic Disparities in Survival-A Review of 4714 @entity1 .
OBJECTIVE: To determine whether or not patients, patient race and ethnicity affect sinonasal tumor, cancer, Cancer survival. STUDY DESIGN: Retrospective database analysis. SETTING: National tumor, cancer, Cancer Institute's Surveillance, Epidemiology, and End Results Database, 1988-2010. SUBJECTS AND METHODS: patients, patient89 cases were extracted according to site codes and histology recode-broad groupings. The cohort was used to calculate disease-specific survival in regard to race and ethnicity. Extracted data were further analyzed through direct comparisons and multivariable Cox regression models controlling for patients, patient , tumor, cancer, Cancer , and treatment characteristics. RESULTS: Unadjusted survival curves for all patients, patient89 showed poorer disease-specific survival for black versus white patients, patient (P = .02), which was eliminated after controlling for tumor, cancer, Cancer characteristics (hazard ratio: 1.02, P = .86). Specifically for sinonasal squamous cell carcinoma , significantly poorer disease-specific survival was found for both black (P = .01) and Hispanic (P = .01) patients, patient as compared with white patients, patient . Similarly, when controlling for tumor, cancer, Cancer characteristics, the disease-specific survival disparity was eliminated for black (hazard ratio: 0.93, P = .59) and Hispanic patients, patient (hazard ratio: 1.01, P = .94). CONCLUSION: Black race is a risk factor for poorer disease-specific survival when all sinonasal histologic subtypes are examined together. Specifically for sinonasal squamous cell carcinoma , both black race and Hispanic ethnicity are risk factors for poorer disease-specific survival. When tumor, cancer, Cancer characteristics are controlled for in this cohort, the survival disparity is eliminated, demonstrating that the disparity can be accounted for exclusively by more advanced disease at presentation, opposed to the more complex effect seen in other subsites of the head and neck.
| null |
2152_biomrc
|
Title: Uterine smooth XXXX of uncertain malignant potential (STUMP): pathology, follow-up and recurrence.
The term smooth uterine muscle of uncertain malignant potential (STUMPs) indicates a group of uterine smooth women, patients, patient1090 (SMTs) that cannot be diagnosed unequivocally as benign or malignant. Diagnosis, surgical management, and follow-up of this neoplasm remain controversial, especially in pre-menopausal women, patients, patient with fertility desire, due to the women, patients, patient46 and prolonged survival rate when compared to women, patients, patient822 . However, recurrence is estimated between 8.7% and 11% and may include delayed-recurrences. We reported five cases of uterine masses treated by surgical procedure diagnosed as STUMP on final pathology. Four women, patients, patient underwent a total abdominal hysterectomy with or without salpingo-oophorectomy. One women, patients, patient underwent excision of uterine mass and subsequent total abdominal hysterectomy plus bilateral salpingo-oophorectomy after the diagnosis of STUMP. All women, patients, patient in our study remained recurrence-free to date (with a follow up period ranging from 6 to 81 months). Based on our experience and in consideration of the lack of consensus regarding the malignant potential, diagnostic criteria, gold-standard treatment and follow-up, we believe that close multidisciplinary management is mandatory in the event of STUMP. We suggest that gynaecologist, dedicated pathologist (with high level of expertize in gynaecological pathology) and oncologist should work as a team in the counselling and management of this neoplasm from detection till completion of follow up. Furthermore, we recommend immunohistochemistry to investigate the overexpression of p16 and p53 in order to identify the cohort of women, patients, patient at increased risk of recurrence who may benefit from more aggressive surgical-oncological strategies.
| null |
4203_biomrc
|
Title: Clinical and histopathological spectrum of @entity1333 in XXXX .
BACKGROUND: Cutaneous manifestations are the most frequent, and often the initial feature of extra-articular involvement in patient, patients with patient, patients2299 . OBJECTIVES: The purpose of the study was to evaluate the clinical and histological spectrum of patient, patients333 and the associated systemic involvement in patient, patients with patient, patients2299 . METHODS: Among 525 patient, patients with rheumatoid arthritis, rheumatoid , 20 tissue specimens with histologically proven patient, patients333 from 11 patient, patients were investigated by studying the types and levels of affected vessels and related clinical features. RESULTS: Small-vessel patient, patients333 identified as dermal necrotizing venulitis was found in 10 patient, patients , clinically characterized by palpable purpura , maculopapular erythema , erythema elevatum diutinum and haemorrhagic blisters . Arteritis, arteritis histologically resembling patient, patients1657 , clinically characterized by subcutaneous nodules, patient, patients2288 , patient, patients94 and deep patient, patients066 was identified in four patient, patients all with systemic complications. Coexistence of venulitis and Arteritis, arteritis was identified in three patient, patients . Different cutaneous vasculitic manifestations often coexisted and recurred in the same patient, patients . Three patient, patients with systemic complications of mononeuritis multiplex (two of three), interstitial pulmonary fibrosis (two of three) and patient, patients661 (one of three) died within 1 year of onset of the patient, patients333 . Immunofluorescence demonstrated vessel wall deposition of IgM and/or complement in six of the seven patient, patients examined. CONCLUSIONS: Features of cutaneous patient, patients2299 overlapping both the characteristics of cutaneous necrotizing venulitis and patient, patients1657 together with coexistence of these different type of patient, patients333 in the same or different lesional skin account for the associated diverse cutaneous vasculitic manifestations. Although dermal venulitis ( patient, patients333 ) was the most common presentation, the presence of patient, patients333 in rheumatoid arthritis, rheumatoid patient, patients did not necessarily indicate a favourable prognosis. Associations with mononeuritis multiplex and bowel involvement had a fatal prognosis, while patient, patients with superficial dermal venulitis without other extra-articular involvement may follow a favourable prognosis.
| null |
112_biomrc
|
Title: XXXX with lung metastasis diagnosed by lung biopsy.
Leydig cell tumor, Leydig cell tumors are very rare and account for only 3% of testicular cancers, testicular tumors and are generally benign. Only less than 0.2% of all testicular cancers, testicular tumors were evidenced by metastatic spread. We report a 34-year-old man visited hospital because of coughing sputum mixed with blood. His chest CT showed bilateral patch clouding opacity. He was suspected with testicular cancers, testicular tumors6 and treated with man667 . However, his symptoms and general condition deteriorated, and he visited our hospital. He had no abnormal findings on physical examination. A chest radiograph showed pneumonia in whole lung and CT showed multiple nodules and diffused ground glass opacities in both lung fields. Lung biopsy confirmed a diagnosis of Leydig cell tumor, Leydig cell tumors with lung metastasis. The diagnosis is based on the histopathology and immunohistochemistry.
| null |
4209_biomrc
|
Title: The predictive value of skin XXXX for late radiation effects in different normal tissues.
Alterations in the microcirculation and parenchymal cell loss are common phenomena after irradiation of different organs. Whether parenchymal cell loss is a process well dissociated from vasculoconnective damage, or a consequence of this, is much debated. However, comprehensive radiopathological studies have shown that vasculoconnective tissue is an important common target for late effects in various organs. Scoring of skin telangiectasia was used by us as a clinical assay of late tissue effects after different dose schedules. All studies were done prospectively with standardized skin area, field size and radiation quality. The patients were scored regularly up to 10 years. The number of patients at risk for a prescribed score versus time was calculated with the life-table method. The late effects after 5 X 2.0 Gy/wk, in the dose range 40 to 70 Gy and after 2 X 4.0 Gy/wk, in the dose range 40 to 56 Gy have been established. The skin dose is 90% of the referred dose. Dose-response curves, relating the proportion of patients with a certain score at a fixed time and radiation dose and dose-latency curves, relating the latent period for a fixed proportion of patients with a certain score and radiation dose, were constructed. The analysis shows that: ED10/5 yr and ED50/5 yr for 5 X 2.0 Gy/wk is 50 Gy and 65 Gy, respectively, for distinct telangiectasia ; The latent period, concerning both a certain frequency and degree of reaction, varies exponentially with dose level; The latent period for 50% of the patients , to obtain a certain score, LP50, is correlated to that for 10%, LP10, with LP50/LP10 = 2.2 +/- 0.2 (S.D.). This correlation is independent of score, total dose, and fractionation; Isoeffective doses for 5 X 2.0 Gy/wk and 2 X 4.0 Gy/wk, determined from the dose-response curves, resulted in the repair exp N between 0.31 and 0.32 and alpha/beta ratio between 2.9 and 3.1 Gy and determined from the dose-latency curves in exp N between 0.30 and 0.32 and alpha/beta ratio between 3.4 and 2.9 Gy. In conclusion, frequent and careful follow-up with registration of normal tissue reactions, until at least 10% of the patients have obtained the prescribed effect, is predictive for the further progression of the late effects. The fractionation characteristics for telangiectasia agree well with those for animal experimental morphological and functional endpoints for late effects in different organs and support the relevance of telangiectasia as a model for predicting late effects.
| null |
2162_biomrc
|
Title: Replacement of antipsychotic and antiepileptic medication by @entity38171 in a @entity1 with XXXX .
We report the case of a 23-year-old woman with VCFS, velocardiofacial syndrome ( VCFS, velocardiofacial syndrome ) and a history of psychotic, psychosis and seizures . She had been treated with conventional antipsychotic and antiepileptic drugs for 10 and 3 years, respectively. However, she continued to experience occasional woman833 and paroxysmal jerking of the extremities. L-alpha-methyldopa 500 mg b.i.d. (later reduced to 250 mg t.i.d.) was added to her regimen. woman833 and seizures stopped shortly. Over the course of approximately 1 year, the previous medications were discontinued without recurrence of psychotic, psychosis and epileptic symptoms. Eventually, improved mental functions and behaviour enabled her transition from living in a licensed residential facility to sharing a private residence with a partner. VCFS, velocardiofacial syndrome is associated with haploinsufficiency of catecholamine -methyltransferase, leading to excessive extraneuronal catecholamine concentrations. Alpha-Methyldopa inhibits catecholamine neurotransmission in a variety of ways. It is possible that the drug compensated for genetically disturbed catecholamine transmission thus achieving beneficial effects in this case.
| null |
115_biomrc
|
Title: Non-myeloablative stem cell transplantation for the treatment of XXXX and life-threatening non-malignant disorders; past accomplishments and future goals.
Allogeneic bone marrow or blood stem cell transplantation (BMT) represents an important therapeutic tool for treatment of otherwise incurable malignant and non-malignant diseases. Until recently, autologous and allogeneic bone marrow or mobilized blood stem cells transplantation were used primarily to replace malignant, genetically abnormal or deficient immunohematopoietic compartment and therefore, highly toxic myeloablative regimen were considered mandatory for more effective eradication of all undesirable host-derived hematopoietic elements. Our preclinical and ongoing clinical studies indicated that much more effective eradication of host immunohematopoietic system cells could be achieved by adoptive allogeneic cell therapy with donor lymphocyte infusion following BMT. Thus, eradication of blood cancer, tumor cells, especially in children, human, patients with children, human, patients860 and less frequently in children, human, patients with other children, human, patients583 , could be frequently accomplished despite complete resistance of such blood cancer, tumor cells to maximally tolerated doses of chemoradiotherapy. Our cumulative experience suggested that graft versus children, human, patients778 (GVL) effects might be a useful tool for eradication of otherwise resistant blood cancer, tumor cells of host origin. Based on the cumulative clinical experience and experimental data in animal models of children, human, patients diseases it appears that induction of host versus graft tolerance as step one, may allow durable engraftment of donor immunocompetent lymphocytes, which may be used for induction of effective biologic warfare against host-type immunohematopoietic cells that need to be replaced, malignant, genetically abnormal or self-reactive alike. Based on the aforementioned rationale, we speculated that the therapeutic benefit of BMT may be improved by using a safer conditioning as part of the transplant procedure, with the goal in mind to induce host versus graft tolerance to enable subsequent induction of GVL, possibly graft versus blood cancer, tumor or even graft versus autoimmunity effects, rather than attempt to eliminate host cells with hazardous myeloablative chemoradiotherapy. The latter hypothesis suggested that effective BMT procedure may be accomplished without lethal conditioning of the host, using new well tolerated non-myeloablative regimen, thus possibly minimizing immediate and late side effects related to myeloablative procedures considered until recently mandatory for conditioning of BMT recipients. Recent clinical data that will be presented suggests that effective BMT procedures may be accomplished with well-tolerated non-myeloablative stem cell transplantation (NST) regimen, with no major children, human, patients37 . Thus, new NST approaches may offer the feasibility of safer BMT procedure for a large spectrum of clinical indications in children, human, patients and elderly individuals without lower or upper age limit, while minimizing procedure-related children, human, patients37 and mortality. Taken together, our cumulative data suggest that high dose chemotherapy and radiation therapy may be successively replaced by a more effective biologic tool, alloreactive donor lymphocytes, thus setting the stage for innovative therapeutic procedures for safer and more effective treatment of children, human, patients in need of BMT.
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2166_biomrc
|
Title: Role of smoking and HbA1c level in periodontitis among insulin-dependent XXXX @entity1 .
OBJECTIVES: The aim was to analyse the role of smoking and HbA1c level in attachment loss, AL ( attachment loss, AL ) and probing depths (PDs) among insulin-dependent diabetic women, patients, patient, men . MATERIAL AND-METHODS: The study subjects were selected from a group of 149 insulin-dependent diabetic women, patients, patient, men and included 64 women, patients, patient, men (39 women, patients, patient, men and 25 women, patients, patient, men ) aged 30 years or older. Data were obtained from women, patients, patient, men records and by clinical examination. The outcome variables were the number of sites with attachment loss, AL and PDs of 5-9 mm. Relative risks (RRs) with 95% confidence intervals (CIs) were estimated using Poisson regression models. RR was adjusted for the number of teeth, dental women, patients, patient, men608 and age. RESULTS: RR for attachment loss, AL among the smokers was 4.15 (95% CI: 2.30-7.63) and that for women, patients, patient, men726 among the smokers was 7.96 (95% CI: 4.91-13.19). HbA1c was not related to attachment loss, AL or women, patients, patient, men726 . Among smokers with HbA1c > 8.5, RR for attachment loss, AL was 12.34 (95% CI: 4.14-39.35), but RR was not elevated for women, patients, patient, men726 . CONCLUSIONS: It can be concluded that the poor metabolic control together with smoking is extremely detrimental for attachment loss, AL .
| null |
2167_biomrc
|
Title: Viral and bacterial patterns induce TLR-mediated sustained XXXX and calcification in aortic valve interstitial cells.
BACKGROUND: Aortic stenosis, degenerative aortic stenosis shares some ethiopathological features with atherosclerosis and increasing evidence links Toll-like receptors (TLRs) to atherogenesis. METHODS: TLR-mediated inflammation and osteogenesis were investigated in human interstitial cells isolated from stenotic and non-stenotic aortic valves. TLR expression and signalling were evaluated by quantitative RT-PCR, flow cytometry, Western blot analysis, ELISA, and cytokine arrays. Osteogenesis was evaluated by measuring alkaline phosphatase activity. RESULTS: Interstitial cells from control valves express most TLRs, being TLR4 the most abundant, whereas cells from stenotic valves express higher TLR4 and TLR2 and lower TLR5 and TLR9 transcript levels. When pro-inflammatory pathways were analyzed, we observed that TLR4 , TLR2 and TLR3 ligands induced an early activation of NF-kB and human435 MAPK activation in cells from control and stenotic valves. Strikingly, when TLRs sensing viral patterns were studied, a sustained TLR3 -mediated activation of NF-kB, a kB-independent induction of catalytically active cyclooxigenase (COX)-2 and ICAM-1 expression, and induction of expression of several chemokines were observed. TLR4 , but not TLR2 , engagement produced a similar but NF-kB-dependent effect. Moreover, TLR3 and TLR4 agonists induced alkaline phosphatase expression and activity. CONCLUSIONS: Exposure of aortic valve interstitial cells to viral and Gram-negative bacteria molecular patterns induces distinct and long-term TLR-mediated pro-inflammatory and pro-osteogenic responses that might be relevant to the pathogenesis of Aortic stenosis, degenerative aortic stenosis .
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2168_biomrc
|
Title: Differences in glomerular leukocyte infiltration between @entity8 and XXXX .
BACKGROUND: An important aspect in glomerular nephritic processes is the enhanced influx of leukocytes into the glomerulus. METHODS: To investigate the mechanisms of intraglomerular leukocyte infiltration in IgA nephropathy, IgA-N ( IgA nephropathy, IgA-N ) and MPGN-I, membranoproliferative glomerulonephritis type I ( MPGN-I, membranoproliferative glomerulonephritis type I ), we immunohistochemically examined the intraglomerular expression of human453 ( human453 , human453 / human454 ), macrophage-1 (Mac-1, Mac-1, CD11b , macrophage-1 (Mac-1, Mac-1, CD11b / human454 ) and ICAM-1, intercellular adhesion molecule-1, CD54 ( ICAM-1, intercellular adhesion molecule-1, CD54 , ICAM-1, intercellular adhesion molecule-1, CD54 ) together with glomerular deposition of C3c and fibrinogen. RESULTS: In IgA nephropathy, IgA-N (n=42), human453 + cells were distributed mainly in glomeruli with intense expression of ICAM-1, intercellular adhesion molecule-1, CD54 , and there was a positive correlation (P<0.001) between the number of human453 + cells and the degree of ICAM-1, intercellular adhesion molecule-1, CD54 expression. macrophage-1 (Mac-1, Mac-1, CD11b + cells had no correlation with glomerular C3c deposition, but had a significant correlation with fibrinogen deposition (P<0.05). The number of human453 + cells was significantly greater than of macrophage-1 (Mac-1, Mac-1, CD11b + cells (P<0.05). The number of human453 + cells was strongly correlated with that of CD68 + cells (P<0.00001). In MPGN-I, membranoproliferative glomerulonephritis type I (n= 43), on the contrary, macrophage-1 (Mac-1, Mac-1, CD11b + cells correlated only with C3c deposition (P<0.001), and they were observed mainly in peripheral loops of glomerular capillaries where C3c was deposited with a similar distribution. However, there was no relationship between human453 + cells and ICAM-1, intercellular adhesion molecule-1, CD54 expression. The number of macrophage-1 (Mac-1, Mac-1, CD11b + cells was greater than that of human453 + cells (P<0.0001), and most macrophage-1 (Mac-1, Mac-1, CD11b + cells were identical to CD15 + cells. CONCLUSION: These results indicate the possibility that different mechanisms may cause glomerular leukocyte infiltration in various forms of human glomerulonephritis . The human453 / ICAM-1, intercellular adhesion molecule-1, CD54 pathway may play an important role in glomerular leukocyte infiltration in IgA nephropathy, IgA-N , while the macrophage-1 (Mac-1, Mac-1, CD11b /complement pathway may be important in MPGN-I, membranoproliferative glomerulonephritis type I . The former may promote mainly the infiltration of CD68 + cells, and the latter may promote that of CD15 + cells. In addition, macrophage-1 (Mac-1, Mac-1, CD11b + cells may act as fibrinogen and complement receptors in IgA nephropathy, IgA-N and MPGN-I, membranoproliferative glomerulonephritis type I , respectively.
| null |
4216_biomrc
|
Title: Laparoscopic and robot-assisted surgery in the management of XXXX .
OBJECTIVES: To review the current role of laparoscopy and robot-assisted laparoscopy for managing patients791 . RESULTS: The contemporary indications for laparoscopic stone management are: anatomical variations in location or shape of the kidney ( pelvic kidney, malrotated kidney, horseshoe kidney , pelvic kidney, malrotated kidney, horseshoe kidney and pelvic kidney, malrotated kidney, horseshoe kidney ); coexisting pathologies, e.g. pelvi-ureteric junction obstruction ; and stones in a renal unit with lower patients695 . The laparoscopic approach allows the simultaneous management of both the pathologies. Symptomatic stones in diverticula not amenable to endourological intervention can be treated with laparoscopy. Large impacted pelvic and ureteric calculi with a functioning renal unit are an indication for laparoscopic ureterolithotomy or pyelolithotomy. This review of current reports suggests that in a selected group of patients with complex stone disease the laparoscopic approach offers good success rates with minimal complications. There are few reports of robotic procedures in stone disease but existing data suggest that it is feasible. CONCLUSION: Laparoscopic surgery is effective for patients290 and offers excellent stone clearance rates with minimal morbidity. Laparoscopic surgery is complementary in managing these stones. Robot-assisted laparoscopic technique of urinary tract stone management is in its early stage of implementation and randomised trials that compare robot assisted outcomes with other minimally invasive techniques are needed.
| null |
2169_biomrc
|
Title: Calprotectin levels in gingival crevicular fluid predict disease activity in XXXX treated for generalized aggressive @entity2521 .
BACKGROUND AND OBJECTIVE: Clinical parameters such as probing depth and bleeding on probing are commonly used for monitoring after periodontal treatment. However, these parameters have poor prognostic utility. The biomarker calprotectin is used to monitor conditions such as inflammatory bowel disease because of its ability to predict disease activity. Levels of calprotectin in gingival crevicular fluid correlate with periodontal disease severity and treatment outcome. The validity of calprotectin as predictor for future periodontal disease activity has not yet been investigated. MATERIAL AND METHODS: Thirty-six subjects with generalized aggressive periodontitis were treated with scaling and root planing (SRP), and with adjunctive antimicrobial medications. Probing depth, clinical attachment level and bleeding on probing were assessed at baseline, and 3 and 6mo after SRP. A gingival crevicular fluid sample was collected from the initially deepest site in each Patients, patients, patient 3mo after SRP and analysed for calprotectin levels. Activity was defined as a probing depth increase of >0.5mm between 3 and 6mo at the sample site. The ability of individual parameters to predict activity was analysed by construction of receiver operating characteristic curves. RESULTS: Nine active sites were identified. Clinical attachment level, probing depth, bleeding on probing and gingival crevicular fluid volume showed no predictive utility [area under the curve (AUC) <0.6, p>0.05]. However, calprotectin concentration (AUC=0.793, p=0.01) and the total amount/sample of calprotectin (AUC=0.776, p=0.02) significantly predicted activity. Patients, patients, patient with calprotectin levels above calculated cut-off values had significantly more active sites than Patients, patients, patient with negative results. CONCLUSION: Calprotectin levels were predictors of disease activity at both site and subject levels. The calculated cut-off values provide a dichotomous basis for prospective evaluation of calprotectin as a diagnostic marker for monitoring periodontal treatment.
| null |
2173_biomrc
|
Title: @entity1760 homeostasis in @entity694 LoVo cells sensitive or resistant to XXXX .
UNASSIGNED: Neoplastic cells accumulate magnesium , an event which provides selective advantages and is frequently associated with TRPM7 overexpression. Little is known about magnesium homeostasis in drug-resistant cancer cells. Therefore, we used the colon cancer LoVo cell model and compared doxorubicin -resistant to sensitive cells. In resistant cells the concentration of total magnesium is higher while its influx capacity is lower than in sensitive cells. Accordingly, resistant cells express lower amounts of the TRPM6 and 7, both involved in magnesium transport. While decreased TRPM6 levels are due to transcriptional regulation, post-transcriptional events are involved in reducing the amounts of TRPM7 . Indeed, the calpain inhibitor calpeptin markedly increases the levels of TRPM7 in resistant cells. In doxorubicin -sensitive cells, silencing TRPM7 shifts the phenotype to one more similar to resistant cells, since in these cells silencing TRPM7 significantly decreases the influx of magnesium , increases its intracellular concentration and increases resistance to doxorubicin . On the other hand, calpain inhibition upregulates TRPM7 , decreases intracellular magnesium and enhances the sensitivity to doxorubicin of resistant LoVo cells. We conclude that in LoVo cells drug resistance is associated with alteration of magnesium homeostasis through modulation of TRPM7 . Our data suggest that TRPM7 expression may be an additional undisclosed player in chemoresistance.
| null |
4222_biomrc
|
Title: [Expression and role of complement regulatory proteins on XXXX gametes and pre-implantation embryos].
human, Human gametes and pre-implantation embryos express selectively several complement regulatory proteins. MCP, CD46, Membrane cofactor protein ( MCP, CD46, Membrane cofactor protein , MCP, CD46, Membrane cofactor protein ) and CD55, decay accelerating factor, DAF ( CD55, decay accelerating factor, DAF , CD55, decay accelerating factor, DAF ) are regulators for C3 convertases and protectin ( human, Human009 ) is an inhibitor of the membrane attack complex. These three proteins were identified on human, Human sperm and found to be functional. CD55, decay accelerating factor, DAF and human, Human009 were both expressed by the plasmic membrane of unfertilized oocytes and pre-implantation embryos. MCP, CD46, Membrane cofactor protein was not present on unfertilized oocytes but appeared at the 6/8 cell-stage embryo when human, Human gene expression first occurs. Complement receptor 1, CD35, CR1 ( Complement receptor 1, CD35, CR1 , Complement receptor 1, CD35, CR1 ) and MHC class I antigens were not found on oocytes neither on embryos. Such a selective expression of complement regulatory proteins associated with the lack of MHC class I antigens may represent an immune protective mechanism by which human, Human gametes and pre-implantation embryos escape from complement-mediated damage during their travel through the female genital tract. Indeed uterine, tubal and follicular fluids contain all the components of the complement cascade, including classical and alternative pathways. Nevertheless participation of MCP, CD46, Membrane cofactor protein and human, Human009 in cell to cell interaction during fertilization and/or implantation cannot be excluded. human, Human009 is an adhesive molecule involved in the rosette phenomena and MCP, CD46, Membrane cofactor protein has been described as the human, Human receptor for measles virus , which binds through a fusion protein. Monoclonal antibodies raised against these two proteins ( MCP, CD46, Membrane cofactor protein and human, Human009 ) are able to inhibit heterospecific fertilization between zona-free hamster oocytes and human, Human spermatozoa suggesting the role of these proteins during fertilization.
| null |
2175_biomrc
|
Title: In vivo modulation of myelopoiesis by @entity1421 . IV. XXXX induction of myelopoietic inhibitory activity.
CM, Conditioned medium ( CM, Conditioned medium ) prepared from bone marrow (BM) or spleen (SPL) cells from mice injected with PGE2 in doses ranging from 0.0001 to 10 micrograms was found to contain an activity inhibitory to the proliferation of granulocyte-monocyte progenitor cells (CFU-GM). This activity was found in medium conditioned for 24 to 48 h, but was not present in medium conditioned for longer time intervals. BM cells from PGE2 -treated mice incubated over a concentration range of 0.1 to 1.0 x 10(6) cells/ml and SPL cells over a range of 1.0 to 10 x 10(6) cells/ml produced CM, Conditioned medium with equivalent degrees of inhibition for CFU-GM proliferation. Titration of this activity revealed a significant inhibitory effect still present at a 1/256 dilution. Inhibitory activity was similar whether or not CM, Conditioned medium was prepared in the presence or absence of FCS. Inhibition of CFU-GM development was approximately equal in the presence of either PWM SPL CM, Conditioned medium or L cell CM, Conditioned medium as sources of CSF activity. Morphologic analysis of CFU-GM revealed an equivalent inhibition of monocyte, monocyte-neutrophil, and neutrophil CFU-GM by the PGE2 -stimulated inhibitory activity. Equivalent picogram amounts of PGE were measured in CM, Conditioned medium derived from BM or SPL cells from either control or PGE2 -treated mice , indicating a low probability that injected PGE2 was carried over in the CM, Conditioned medium and contributed to CFU-GM inhibition. Protease digestion of BM or SPL cell CM, Conditioned medium from PGE2 -treated mice revealed a loss of inhibitory activity after trypsin, chymotrypsin, pronase, and neuraminidase treatment. Inhibitory activity was also ablated by heat treatment at 56 degrees C for 30 min and 100 degrees C for 5 min. Acrylamide -agarose gel filtration of BM CM, Conditioned medium revealed an active inhibitory fraction in the Mr range of 5.5 to 8.0 kDa. The results of the present study suggest that one of the mechanisms by which PGE2 exerts its in vivo myelopoietic inhibitory action may be by stimulating the production of an inhibitory factor or factors from BM and SPL cells.
| null |
128_biomrc
|
Title: Histopathologic features and risk factors for @entity5 , @entity1373 , and XXXX in @entity1478 .
OBJECTIVE: The purpose of this study was to determine the rate of benign, cancerous, malignancy072 , and malignant patients478 and whether clinical data can predict histopathologic outcome. STUDY DESIGN: Five hundred nine patients with patients478 who consecutively underwent hysteroscopic removal of patients478 48 months were identified from our gynecologic oncology surgical database. Medical reports provided clinical data. Statistical analysis was performed. RESULTS: Histologically, 358 patients127 (70.3%) were benign; 131 patients127 (25.7%) had simple or patients869 , 16 patients127 (3.1%) had patients373 with atypia, and 4 patients127 (0.8%) were cancerous, malignancy . patients127 were divided into group A and group B, according to the risk of cancerous, malignancy (group A, benign; group B, atypical cancerous, malignancy072 and cancerous, malignancy ). Age, menopause status, and patients01 were associated significantly with group B. CONCLUSION: patients478 rarely become malignant, but cancerous, malignancy072 changes are more common. Age, menopause status, and patients01 may increase the risk of premalignant and malignant patients127 . To patients127 and a reliable histologic analysis, operative hysteroscopy should be offered to symptomatic patients or to patients with risk factors.
| null |
129_biomrc
|
Title: Prevalence of @entity2299 in normoglycemic subjects with newly diagnosed XXXX in Abuja, Nigeria.
BACKGROUND: High blood pressure, high blood pressure and dyslipidemia additively increases the risk of cardiovascular disease . There is a high prevalence of High blood pressure, high blood pressure in Nigeria, but there are little data regarding the prevalence of dyslipidemia in subjects with High blood pressure, high blood pressure . OBJECTIVE: In this observational prospective study, we examined the prevalence of dyslipidemia in newly diagnosed normoglycemic subjects with High blood pressure, high blood pressure . METHODS: A total of 171 subjects presenting with High blood pressure, high blood pressure for the first time in the cardiology and nephrology clinics at the University of Abuja Teaching Hospital were studied. Height, weight, and blood pressure were measured. Total Cholesterol, cholesterol , low-density lipoprotein Cholesterol, cholesterol (LDL-C), and high-density lipoprotein Cholesterol, cholesterol (HDL-C) were determined in fasting plasma. The total Cholesterol, cholesterol /HDL-C and non-HDL-C values were calculated. These measures were then classified according to the 2001 report of the National Cholesterol, cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol, cholesterol in Adults. RESULTS: Of the 171 subjects studied, 84 (49%) were male and 87 (51%) were female. Low HDL-C was present in 71 (45.8%), elevated LDL-C in 29 (17%), elevated total Cholesterol, cholesterol in 19 (11.1%), and elevated triglyceride in 13 (7.6%), whereas eight (4.7%) of the study population had combined elevated total Cholesterol, cholesterol and triglyceride . Female subjects had higher total Cholesterol, cholesterol and lower HDL-C than male subjects, but these differences were not statistically significant. Obese subjects, compared to the nonobese, had significantly higher LDL-C and total Cholesterol, cholesterol /HDL-C ratios in males and significantly higher triglyceride levels in females. CONCLUSIONS: Given the prevalence of dyslipidemia seen in this study, we suggest that fasting lipid measurements should be performed in all Nigerians with High blood pressure, high blood pressure . These data suggest the need for health education and lifestyle modifications in hypertensive Nigerians to reduce both types of risk factors.
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4226_biomrc
|
Title: Use of intracardiac recordings to determine the site of XXXX in @entity4710 .
Paroxysmal supraventricular tachycardia most often results from atrioventricular (AV) reentry using an accessory AV pathway ( Wolff-Parkinson-White syndrome ) or reentry within the region of the AV node. In AV reentry, using an accessory pathway, suppression of the tachycardia may be achieved by depressing either anterograde AV nodal conduction or retrograde accessory pathway conduction. Intracardiac recordings and programmed electrical stimulation have established that beta-adrenergic antagonists and calcium channel blockers principally affect AV nodal conduction (anterograde limb of the reentrant circuit), whereas class IA and IC agents principally affect the accessory AV pathway (retrograde limb). Pharmacologic therapy has been more effective when directed at the limb in which conduction is most marginal at the tachycardia rate (weak limb). In individual patients , intracardiac recordings and programmed electrical stimulation can be used to identify the weak limb, indicating the class of agents most likely to be effective. Specialized techniques allowing direct recording of accessory pathway activation suggest that limitations in accessory pathway conduction may be explained by anatomic impediments. Conduction is most limited at the atrial interface of the accessory pathway in some patients , whereas in others the ventricular interface may be the limiting factor. Class IA and IC agents appear to have the greatest effect at sites where conduction is most tenuous, i.e., at the anatomic impediments. Similar considerations apply to AV nodal reentry. Anterograde slow AV nodal pathway conduction is most often depressed by digitalis preparations, beta-adrenergic antagonists, and calcium channel blockers, whereas retrograde fast AV nodal pathway conduction is more often depressed by class IA and IC agents. Intracardiac recordings and programmed electrical stimulation can also be used in these patients to identify the weak limb and direct pharmacologic therapy. Direct catheter recordings of AV nodal conduction remain elusive, limiting knowledge of the different conduction properties of the anterograde and retrograde limbs and the site(s) of drug action . Studies in progress, comparing the retrograde AV nodal conduction time during tachycardia with that during ventricular pacing at the same rate, suggest that the His bundle may be incorporated in the reentrant circuit in some patients . It appears that patients855 more readily depresses retrograde fast pathway conduction in these patients than in those in whom the His bundle does not form part of the reentrant circuit, but the reasons for this are unknown.
| null |
4227_biomrc
|
Title: XXXX Anesthesia Is Associated With Shortened Length of Hospital Stay Following Orthognathic Surgery.
PURPOSE: To evaluate the impact of induced hypotensive, Hypotensive anesthesia on length of hospital stay (LOS) for patients, patient undergoing maxillary Le patients, patient96 in isolation or in combination with mandibular orthognathic surgery. MATERIALS AND METHODS: A retrospective cohort study design was implemented and patients, patient undergoing a Le patients, patient96 as a component of orthognathic surgery at the Mayo Clinic from 2010 through 2014 were identified. The primary predictor variable was the presence of induced hypotensive, Hypotensive anesthesia during orthognathic surgery. hypotensive, Hypotensive anesthesia was defined as at least 10 consecutive minutes of a mean arterial pressure no higher than 60 mmHg documented within the anesthetic record. The primary outcome variable was LOS in hours after completion of orthognathic surgery. The secondary outcome variable was the duration of surgery in hours. Multiple covariates also abstracted included patients, patient age, patients, patient gender, American Society of Anesthesiologists score, complexity of surgical procedure, and volume of intraoperative fluids administered during surgery. Univariable and multivariable models were developed to evaluate associations between the primary predictor variable and covariates relative to the primary and secondary outcome variables. RESULTS: A total of 117 patients, patient were identified undergoing Le Fort I orthognathic surgery in isolation or in combination with mandibular surgery. Induced hypotensive, Hypotensive anesthesia was significantly associated with shortened LOS (odds ratio [OR] = 0.33; 95% confidence interval [CI], 0.12-0.88; P = .026) relative to patients, patient with normotensive regimens. This association between hypotensive, Hypotensive anesthesia and LOS remained statistically significant in a subgroup analysis of 47 patients, patient in whom isolated Le Fort I surgery was performed (OR = 0.13; 95% CI, 0.03-0.62; P = .010). Induced hypotensive, Hypotensive anesthesia was not statistically associated with shorter duration of surgery. CONCLUSION: Induced hypotensive, Hypotensive anesthesia represents a potential factor that minimizes postoperative LOS for patients, patient undergoing routine maxillary orthognathic surgery alone or in combination with mandibular procedures. hypotensive, Hypotensive anesthesia does not appear to be effective in minimizing the duration of surgery within this same patients, patient population.
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4225_biomrc
|
Title: Evolution of ocular manifestations in XXXX : a long-term study of a population treated with @entity8488 .
BACKGROUND: cystinosis, nephropathic cystinosis, Nephropathic cystinosis is characterized by an accumulation of patients, patient434 crystals within most body tissues. Renal transplantation and oral cysteamine have improved the general prognosis of the disease, and ocular manifestations are now the most common complication. This long-term follow-up study describes the sequence of ocular manifestations in patients, patient with cystinosis, nephropathic cystinosis, Nephropathic cystinosis treated with oral and topical cysteamine . METHODS: Data were recorded for all patients, patient with cystinosis, nephropathic cystinosis, Nephropathic cystinosis examined between 1980 and 2000. For each patients, patient , photophobia and visual acuity, visual impairment were evaluated and slit-lamp and fundus examinations were performed. For some patients, patient , an electroretinogram was also performed. RESULTS: Twenty-nine patients, patient were observed during this period. They received oral and topical cysteamine . Photophobia and loss of visual acuity, visual impairment generally began by 10 years of age but were severe only after 15 years of age. Peripheral corneal epithelial infiltration appeared in the first few years of life. Infiltration evolved toward the depth and center of the cornea during the second decade of life. Retinopathy was present in 51.7% of the patients, patient , with 3 cases of patients, patient020 and 3 cases of flattening on electroretinogram. CONCLUSIONS: Photophobia and corneal infiltration , although generally severe after 15 years of age, could be treated with topical cysteamine and corneal transplantation. Retinal infiltration, previously described as frequent and potentially blinding, is currently observed in only half of these patients, patient , with mild visual acuity, visual impairment . This could be related to the treatment with oral cysteamine reaching the retinal vascularization.
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4231_biomrc
|
Title: Analysis of a Functional @entity650 Gene Polymorphism in @entity1530 Associated and Intermediate @entity1596 Gives New Insight in Disease Pathogenesis and Commonality with Other XXXX .
Purpose. IL-6, Interleukin 6 ( IL-6, Interleukin 6 ) plays a crucial role in both adaptive and innate immunity. The patients5147 gene polymorphism of IL-6, Interleukin 6 is associated with various autoimmune diseases , like patients5 . Methods. 134 patients with patients530 positive iridocyclitis , 84 patients with intermediate patients596 , 132 controls, and 65 patients530 positive controls were recruited for the present case-control study. Main outcome measures were genotype distribution and allelic frequencies determined by polymerase chain reaction. Results. The frequency of carriers of the minor allele for patients5147 was significantly higher in patients with intermediate patients596 compared to controls (p = 0.04; OR: 1.46; CI: 1.02-2.11). Frequencies of the minor allele for patients5147 did not differ significantly in patients with patients530 associated patients596 when compared to controls (p > 0.05). Conclusion. These findings further deepen our understanding of the commonality between patients5 and intermediate patients596 . Given the functionality of the investigated polymorphism, new pathophysiological insights are gained that help to evaluate possible therapeutic targets.
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2184_biomrc
|
Title: Simple enucleation is equivalent to traditional partial nephrectomy for XXXX : results of a nonrandomized, retrospective, comparative study.
PURPOSE: The excision of the renal tumor with a substantial margin of healthy parenchyma is considered the gold standard technique for partial nephrectomy. However, simple enucleation showed excellent results in some retrospective series. We compared the oncologic outcomes after standard partial nephrectomy and simple enucleation. MATERIALS AND METHODS: We retrospectively analyzed 982 patients who underwent standard partial nephrectomy and 537 who had simple enucleation for localized patients358 at 16 academic centers between 1997 and 2007. Local recurrence, cancer specific survival and progression-free survival were the main outcomes of this study. The Kaplan-Meier method was used to calculate survival functions and differences were assessed with the log rank statistic. Univariable and multivariable Cox regression models addressed progression-free survival and cancer specific survival. RESULTS: Median followup of the patients undergoing traditional partial nephrectomy and simple enucleation was 51 37.8 and 54.4 36 months, respectively (p = 0.08). The 5 and 10-year progression-free survival estimates were 88.9 and 82% after standard partial nephrectomy, and 91.4% and 90.8% after simple enucleation (p = 0.09). The 5 and 10-year cancer specific survival estimates were 93.9% and 91.6% after standard partial nephrectomy, and 94.3% and 93.2% after simple enucleation (p = 0.94). On multivariable analysis the adopted nephron sparing surgery technique was not an independent predictor of progression-free survival (HR 0.8, p = 0.55) and cancer specific survival (HR 0.7, p = 0.53) when adjusted for the effect of the other covariates. CONCLUSIONS: To our knowledge this is the first multicenter, comparative study showing oncologic equivalence of standard partial nephrectomy and simple enucleation.
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137_biomrc
|
Title: Systematic review of preoperative, intraoperative and postoperative risk factors for XXXX .
BACKGROUND: renal disease34 ( renal disease34 ) represents a dreaded complication following colorectal surgery, with a prevalence of 1-19 per cent. There remains a lack of consensus regarding factors that may predispose to renal disease34 and the relative risks associated with them. The objective was to perform a systematic review of the literature, focusing on the role of preoperative, intraoperative and postoperative factors in the development of colorectal ALs . METHODS: A systematic review was performed to identify adjustable and non-adjustable preoperative, intraoperative and postoperative factors in the pathogenesis of renal disease34 . Additionally, a severity grading system was proposed to guide treatment. RESULTS: Of 1707 papers screened, 451 fulfilled the criteria for inclusion in the review. Significant preoperative risk factors were: male sex, American Society of Anesthesiologists fitness grade above II, renal disease , co-morbidity and history of radiotherapy. Tumour-related factors were: distal site, size larger than 3 cm, advanced stage, emergency surgery and metastatic disease. Adjustable risk factors were: smoking, obesity , poor nutrition, obesity5 excess, immunosuppressants and bevacizumab. Intraoperative risk factors were: blood loss /transfusion and duration of surgery more than 4 h. Stomas lessen the consequences but not the prevalence of renal disease34 . In the postoperative period, CT is the most commonly used imaging tool, with or without rectal contrast, and a C-reactive protein level exceeding 150 mg/l on day 3-5 is the most sensitive biochemical marker. A five-level classification system for renal disease34 severity and appropriate management is presented. CONCLUSION: Specific risk factors and their potential correction or indications for stoma were identified. An renal disease34 severity score is proposed to aid clinical decision-making.
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2185_biomrc
|
Title: Anti- @entity7 in treatment of central XXXX .
Macular edema along with macular ischemia is responsible for decreased visual acuity in central retinal vein occlusion . Bevacizumab (Avastin, Genentech) blocks vascular endothelial growth factor, VEGF ( vascular endothelial growth factor, VEGF ) induced hyperpermeability of blood vessels. In this prospective case series we investigated the efficacy of anti- vascular endothelial growth factor, VEGF treatment in reduction of central retinal thickness (CRT) and improvement in visual acuity (VA). 25 patients were followed up for 12 months and treated monthly with intravitreal bevacizumab. VA and CRT were measured at each visit. Treatment was discontinued as the peak improvement of either parameter was reached and reinstituted in case of deterioration/recurrence of edema . Study endpoints included: VA using ETDRS charts, CRT and number of injections at 12 months. Mean VA from all 25 patients increased by 3.1 logMAR lines (p < 0.05 compared to baseline). The improvement of VA after bevacizumab injection was in correlation with a decrease in CRT In subgroup analyses, patients receiving bevacizumab injection within the first 3 months after CRVO showed an average VA gain of 4.2 logMAR lines. Mean of 4.5 injections was needed to control the disease during the follow-up period. Bevacizumab treatment was effective in VA and reducing CRT. It appears from subgroup analysis that initiation of treatment early in the course of disease produced better functional outcome. Several injections were needed to control the disease. Regular OCT examinations and retreatment are advised in order to maintain initially reached VA.
| null |
2187_biomrc
|
Title: Correlation between radiographic severity of XXXX and future disease progression. Results from a 3-year prospective, placebo-controlled study evaluating the effect of @entity4823 .
OBJECTIVE: To investigate the relationship between baseline radiographic severity of knee osteoarthritis ( OA ) and the importance of long-term joint space narrowing. DESIGN: Sub-analysis from a three-year randomized, placebo-controlled, prospective study, of 212 Patients, patients with knee OA , recruited in an osteoarthritic outpatient clinic and having been part of a study evaluating the effect of glucosamine sulfate on symptom and structure modification in knee OA . MATERIAL AND METHODS: Measurements of mean joint space width (JSW), assessed by a computer-assisted method, were performed at baseline and after 3 years, on weightbearing anteroposterior knee radiographs. RESULTS: In the placebo group, baseline JSW was significantly and negatively correlated with the joint space narrowing observed after 3 years (r=-0.34, P=0.003). In the lowest quartile of baseline mean JSW (<4.5mm), the JSW increased after 3 years by (mean (S.D.)) 3.8% (23.8) in the placebo group and 6.2% (17.5) in the glucosamine sulfate group. The difference between the two groups in these Patients, patients with the most severe OA at baseline was not statistically significant (P=0.70). In the highest quartile of baseline mean JSW (>6.2mm), a joint space narrowing of 14.9% (17.9) occurred in the placebo group after 3 years while Patients, patients from the glucosamine sulfate group only experienced a narrowing of 6.0% (15.1). Patients, patients with the most severe OA at baseline had a RR of 0.42 (0.17-1.01) to experience a 0.5mm joint space narrowing over 3 years, compared to those with the less affected joint. In Patients, patients with mild OA , i.e. in the highest quartile of baseline mean JSW, glucosamine sulfate use was associated with a trend (P=0.10) towards a significant reduction in joint space narrowing. CONCLUSION: These results suggest that Patients, patients with the less severe radiographic knee OA will experience, over 3 years, the most dramatic disease progression in terms of joint space narrowing. Such Patients, patients may be particularly responsive to structure-modifying drugs.
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4238_biomrc
|
Title: Endoscopic XXXX for removal of @entity2877 : indications, advantages, complications, and long-term follow-up results.
Endoscopic papillary balloon dilation (EPBD) is an alternative method of endoscopic sphincterotomy (EST). Although concerns regarding post-procedure pancreatitis have been expressed, EPBD has come to be recognized as an effective and safe method for stone removal in specific cases. To analyze the proper indications, ideal methods, complications, and long-term follow-up results for EPBD, we reviewed articles about EPBD located through a search of the PubMed data base. We analyzed the ballooning methods, indications, results and complications of EPBD among the articles found and compared the results with those of EST. We considered the authors' own clinical experience and knowledge in developing recommendations for EPBD. EPBD showed similar efficacy and safety for the removal of choledocholithiasis to that of EST. Although large or multiple stones were difficult to remove by EPBD, it was safer and easier to apply in patients with patients003 or abnormal anatomy. To prevent severe pancreatitis , excessive ballooning and impractical cannulation should be avoided, and precut sphincterotomy or adjuvant prophylaxis should be considered. Due to its preservation of the sphincter of Oddi, EPBD is expected to have fewer long-term complications, such as stone recurrence, cholangitis and cholecystitis . In conclusion, EPBD appears to be safe and effective for the treatment of choledocholithiasis with proper selection of ballooning methods and patients .
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2191_biomrc
|
Title: Elevated Serum MicroRNA XXXX in Moyamoya Disease.
BACKGROUND: Few studies have examined the relationship between mircroRNAs and moyamoya disease, MMD ( moyamoya disease, MMD ). We performed a study of the significance of patients, people0075 expression in the serum of moyamoya disease, MMD patients, people . METHODS: The experimental group includes 49 moyamoya disease, MMD patients, people , and the control group consists of 30 normal patients, people , 20 patients, people779 patients, people , 20 massive cerebral infarction patients, people , 20 nonmassive cerebral infarction patients, people , and 20 neurological autoimmune disease patients, people . Let-7 family levels were determined by polymerase chain reaction. A dual luciferase assay was used to test whether patients, people0075 recognized the 3'UTR of RNF213 . RESULTS: The expression level of patients, people0075 in moyamoya disease, MMD patients, people is higher than that observed in the control groups (P < .001). The luciferase assay results indicated that patients, people0075 could diminish luciferase activity from a reporter vector containing the 3'-UTR of RNF213 (P < .05). The suppression of luciferase activity is not found in mutRNF213 (P > .05). CONCLUSIONS: Increased expression of patients, people0075 in moyamoya disease, MMD patients, people may contribute to moyamoya disease, MMD pathogenesis by targeting RNF213 . Thus, patients, people0075 may be a potential biomarker for the diagnosis of moyamoya disease, MMD .
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2193_biomrc
|
Title: The influence of age on operative mortality and long-term relative survival following emergency XXXX .
OBJECTIVE: To study operative mortality and long-term survival following emergency operations for women, patients028 . DESIGN: Retrospective survey in a university hospital. MATERIALS: Two hundred and twenty-seven women, patients with median age 72 years, (17% women, patients ). METHODS: Founded on data from the Norwegian Registrar's Office, operative mortality and long-term survival was estimated using the life-table method. Expected survival for demographically matched subgroups was calculated from death rate tables issued by the Norwegian Central Bureau of Statistics. RESULTS: Operative mortality was 41% for the 175 women, patients with women, patients756 and 17% for the 52 with imminent rupture . The 6-year survival rate was 61% for all the successfully operated women, patients , and not different from that of a demographically matched population. For the women, patients of 72 years or older the 6-year survival rate was 53%. This was equal to that of an age and sex matched population. The younger women, patients had an observed 6-year survival rate of 64%, which was significantly lower than the expected of 84%. The standard mortality rate for this group was 2.25. No statistically significant difference in long-term survival was detected between the two age groups. CONCLUSIONS: Age at the time of the operation for a symptomatic women, patients028 does not seem to influence long-term survival. Consequently, younger women, patients experience a higher relative mortality compared to the older.
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2199_biomrc
|
Title: Association of serum soluble receptor for advanced glycation end-products with subclinical XXXX : the Northern Manhattan Study (NOMAS).
OBJECTIVE: Serum levels of the soluble receptor for advanced glycation end-products (sRAGE) have been associated with risk of cardiovascular disease . We hypothesized that sRAGE levels are associated with subclinical cerebrovascular disease in an ethnically diverse population. METHODS: Clinically stroke -free participants in the multi-ethnic Northern Manhattan Study (NOMAS) underwent brain MRI to quantify subclinical brain infarcts (SBI) and white matter hyperintensity volume (WMHV) (n = 1102). Serum levels of sRAGE were measured by ELISA. Logistic and multiple linear regression were employed to estimate associations of sRAGE with SBI and WMHV, after adjusting for demographics and vascular risk factors. RESULTS: Median sRAGE levels were significantly lower in Hispanics (891.9 pg/ml; n = 708) and non-Hispanic blacks (757.4 pg/ml; n = 197) than in non-Hispanic whites (1120.5 pg/ml; n = 170), and these differences remained after adjusting for other risk factors. Interactions were observed by race-ethnicity between sRAGE levels and MRI measurements, including for SBI in Hispanics (p = 0.04) and WMHV among blacks (p = 0.03). In Hispanics, increasing sRAGE levels were associated with a lower odds of SBI, with those in the upper sRAGE quartile displaying a 50% lower odds of SBI after adjusting for sociodemographic and vascular risk factors (p = 0.05). Among blacks, those in the upper quartile of sRAGE had a similarly reduced increased risk of SBI (p = 0.06) and greater WMHV (p = 0.04). CONCLUSION: Compared to whites, Hispanics and blacks have significantly lower sRAGE levels, and these levels were associated with more subclinical brain disease . Taken together, these findings suggest sRAGE levels may be significantly influence by ethnicity. Further studies of sRAGE and stroke risk, particularly in minorities, are warranted.
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4251_biomrc
|
Title: XXXX in Ontario, 1977-1987: population-based results.
A retrospective review was carried out to study children, patients , not more than 16 years old, with a confirmed diagnosis of medulloblastoma , who were residents of the Province of Ontario at the time of diagnosis between 1977 and 1987 inclusive. The provincial RCC, Regional Cancer Centres, Cancer, tumour registry provided the population database. One hundred and eight children, patients with medulloblastoma were identified of whom 72 (67%) were initially treated at University of Toronto Centres and 36 (33%) at other Health Science Centres, hospitals, and RCC, Regional Cancer Centres, Cancer, tumour ( RCC, Regional Cancer Centres, Cancer, tumour ) in Ontario. The hospital/ RCC, Regional Cancer Centres, Cancer, tumour Centre records were reviewed. The 5-year relapse-free survival (RFS) for all children, patients treated in Ontario was 58% (SE = 5%). Those treated in Toronto had a 5-year RFS of 65% (SE = 6%) compared to 44% (SE = 8%) for those treated in other RCCs in the province (P = 0.02). Relapse-free survival for the RCCs ranged from 25 to 60%, with a trend for improved survival with increasing centre size. Univariate analysis of determinants of relapse-free survival for all 108 children, patients showed the following variables to be significant: T-stage (Tx + T1 + T2 vs. children, patients2817 + T3B) P = 0.0004, M-stage (M0 + Mx vs. M1-4) P = 0.0006, extent of resection (total vs. less than total) P = 0.002, radiotherapy (craniospinal irradiation and posterior fossa boost vs. other) P = 0.02, and treatment centre (Toronto centres vs. RCC, Regional Cancer Centres, Cancer, tumour ) P = 0.02. Cases treated at centres outside metropolitan Toronto had a nearly two-fold (relative risk = 1.93; 95% confidence interval = 1.07, 3.47) greater risk of recurrence or death than those seen in Toronto. However, in multivariate analysis this difference was not quite significant (P = 0.07) after controlling for stage (T and M), extent of resection, meningitis , and gender. These data suggest that children, patients with medulloblastoma should be referred for treatment to large centres with major pediatric neurosurgical and oncology resources.
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4253_biomrc
|
Title: [The adverse effects of smoking on healing of open XXXX ].
OBJECTIVE: Survey of the influence of smoking on the healing of open patients74 . DESIGN: Retrospective. METHOD: During the period 1 January 1994 to 31 December 2000, 168 patients were treated at the Department of Surgery (University Medical Center Utrecht, the Netherlands) due to an open patients74 ; 118 patients with 125 patients74 were included in the study. On the basis of their smoking behaviour, patients were classified as 'non-smokers' or 'smokers'. For all of the patients it was ascertained whether or not complications had occurred during treatment. Whether the patients74 had consolidated was determined on the basis of clinical and radiological criteria. RESULTS: Seventy-two (61%) patients were non-smokers and 46 were smokers. The non-smokers had 77 open patients74 and the smokers 48. Initial therapy was identical and wound infections occurred with similar incidences in both groups. However, there was a statistically significant difference in the incidence of osteitis between both groups: 7 (9%) times in the non-smokers and 13 (27%) times in smokers (p = 0.04). The mean time to consolidation was 26 weeks for non-smokers and 33 weeks for smokers (p = 0.04). Smokers remained longer in hospital and underwent more re-operations. CONCLUSIONS: Smoking was negatively associated with the healing of open patients74 . In smoking patients , the time to consolidation was on average statistically significantly longer and they suffered more from osteitis .
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2207_biomrc
|
Title: Administration of the selective cyclooxygenase (COX)-2 inhibitor etodolac prolongs cardiac allograft survival in a XXXX model.
Etodolac, a selective mice, mouse96 ( mice, mouse96 ) inhibitor, is a non-steroidal anti-inflammatory drug. mice, mouse96 is a key factor in the progression of inflammation . Although inflammation is an essential pathologic feature of cardiac allograft rejection, the role of mice, mouse96 in this process remains unclear. The aim of this study was to investigate the expression of COX and the effects of etodolac in a mice, mouse cardiac allograft model. Balb/c mice, mouse (H-2d) were used as recipients and C57BL/6 (H-2b) mice, mouse as heart donors. Heart function was evaluated daily after transplantation by regular abdominal palpation of the heart and by laparotomy in cases where the beating became weak. Rejection was defined as total cessation of cardiac muscle contraction. mice, mouse96 expression was analyzed by immunohistochemistry. Cardiac isograft was well tolerated (>150 days, n=5), while non-treated cardiac allograft was rapidly rejected (mean 10.9 2.4, n=7). In the etodolac-treated cardiac allograft (10 mg/kg/day by hypodermic injection), survival was extended to 18.53 2.1 days (n=7). The necrotic area and the grade of mice, mouse96 immunostaining were more significantly reduced in the etodolac-treated cardiac allograft than in the non-treated cardiac allograft at day 14. These results indicate that etodolac contributes to protection against rejection after heart transplantation. Etodolac could therefore be used to suppress graft rejection by means of its anti-inflammatory properties.
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159_biomrc
|
Title: XXXX with use of a peristernal cable-tie closure system: a retrospective case series.
BACKGROUND: Deep sternal wound infections are a rare but serious complication after median sternotomy. We evaluated the incidence of deep sternal wound infection associated with two techniques for sternal closure. METHODS: In this retrospective case series, we recorded the method of sternal closure in consecutive patients undergoing a variety of cardiothoracic surgical procedures. Sternal closure in the historical control group was performed using trans-sternal, stainless-steel wire sutures; subsequent patients were closed using wire sutures in conjunction with a novel, peristernal cable-tie closure system to reinforce the corpus sterni. Perioperative care was standardized between groups. Demographics, risk factors, and postoperative outcomes were analyzed. RESULTS: Between July 2010 and July 2014, 609 consecutive adult patients underwent sternal closure following open median sternotomy at a single hospital in Mobile, Alabama. Sternal closure was accomplished with wire sutures in the first 309 patients and with cable-tie reinforcement in the subsequent 300 patients . Baseline characteristics were comparable between groups, except that the cable-tie group exhibited greater preoperative comorbidity. Mean body mass index was comparable between groups (30.2 6.6 kg/m(2) wire suture versus 30.5 7.7 cable-tie, p = 0.568). Deep sternal wound infection occurred in 2.6 % (8/309) patients in the wire-suture group, whereas no deep sternal wound infections were observed in the cable tie group (p = 0.008). CONCLUSIONS: The peristernal cable-tie system was a simple and reliable method for sternal closure after open median sternotomy, and was associated with a reduced risk of deep sternal wound infection , even in an obese and comorbid population.
| null |
160_biomrc
|
Title: Role of Nox inhibitors plumbagin, ML090 and gp91ds-tat peptide on homocysteine thiolactone induced XXXX .
UNASSIGNED: Antioxidants have not reduced the burden of cardiovascular disease , and current evidence suggests a beneficial role of oxidative stress, via NADPH oxidase (Nox) upregulation, in endothelial function. Homocysteine thiolactone (HcyT) induces HcyT induced blood vessel dysfunction, blood vessel dysfunction and this correlates with increased vascular oxidative stress. AIMS: To determine if pharmacological inhibition of Nox could impair HcyT induced blood vessel dysfunction, blood vessel dysfunction . METHODS: Abdominal aorta were excised from New Zealand White rabbits (n=6), cut into rings and sequentially mounted in organ baths. Rings were preincubated with 0.55 M homocysteine thiolactone for 1hr, or combinations of putative Nox inhibitors (plumbagin for Nox4 , gp91ds-tat for Nox2 , and ML090 for Nox1 ), 30 minutes prior to the addition of HcyT, followed by a dose response curve to acetylcholine on phenylephrine preconstricted rings. RESULTS: Plumbagin, ML090 + gp91ds-tat and HcyT reduced responses to acetylcholine, and Plumbagin + Hcyt caused constriction to acetylcholine, which was normalised to plumbagin by ML090. Plumbagin + ML090 or plumbagin + gp91ds-tat completely impaired the effect of acetylcholine. ML090 inhibited the effect of HcyT on reduced response to acetylcholine, whereas gp91ds-tat had no effect. CONCLUSION: Inhibition of Nox1 prevents, whereas inhibition of Nox4 worsens acetylcholine induced blood vessel relaxation caused by HcyT, while Nox2 inhibition has no effect. However combinations of Nox inhibitors worsen acetylcholine induced blood vessel relaxation. These results suggest that there is cross-talk between Nox isoforms during physiological and pathophysiological processes. This article is protected by copyright. All rights reserved.
| null |
2211_biomrc
|
Title: Cohort study on effects of parathyroid surgery on multiple outcomes in XXXX .
OBJECTIVES: To assess the effects of surgery compared with conservative treatment (no surgery) for primary hyperparathyroidism . DESIGN: Cohort study. SETTING: Nationwide Danish cohort. Patients, PARTICIPANTS, patients : 3213 Patients, PARTICIPANTS, patients , mean age 61 (SD 16) years, with a diagnosis of primary hyperparathyroidism between 1980 and 1999. 1934 (60%) underwent surgery and 1279 (40%) were treated conservatively. MAIN OUTCOME MEASURES: Occurrence of Patients, PARTICIPANTS, patients74 , Patients, PARTICIPANTS, patients70 , kidney or urinary tract stones, malignant diseases83 , angina pectoris , cardiac arrhythmias , Patients, PARTICIPANTS, patients01 , heart failure , stroke , acute pancreatitis , gastric ulcers, stomach or duodenal ulcers , painful muscle disorders, muscle pain , malignant diseases , Patients, PARTICIPANTS, patients46 , and mortality. RESULTS: At diagnosis of primary hyperparathyroidism , Patients, PARTICIPANTS, patients who subsequently underwent surgery had a lower prevalence of previous Patients, PARTICIPANTS, patients74 (odds ratio 0.64, 95% confidence interval 0.51 to 0.80), malignant diseases83 (0.59, 0.42 to 0.83), stroke (0.57, 0.37 to 0.88), Patients, PARTICIPANTS, patients46 (0.54, 0.31 to 0.94), and painful muscle disorders, muscle pain (0.44, 0.26 to 0.76), whereas Patients, PARTICIPANTS, patients290 (2.49, 1.93 to 3.23) and acute pancreatitis (2.77, 1.33 to 5.76) were more prevalent. After diagnosis, the risks of Patients, PARTICIPANTS, patients74 (hazards ratio 0.69, 0.56 to 0.84) and gastric ulcers, stomach or duodenal ulcers (0.59, 0.41 to 0.84) were lower in Patients, PARTICIPANTS, patients treated surgically than those treated conservatively. Events involving kidney or urinary tact stones were more prevalent in Patients, PARTICIPANTS, patients treated surgically than Patients, PARTICIPANTS, patients treated conservatively (1.87, 1.30 to 2.68). Mortality was lower in Patients, PARTICIPANTS, patients treated surgically (0.65, 0.57 to 0.73). CONCLUSIONS: Patients, PARTICIPANTS, patients treated surgically for primary hyperparathyroidism have a lower prevalence of Patients, PARTICIPANTS, patients74 and gastric ulcers, stomach or duodenal ulcers than Patients, PARTICIPANTS, patients treated conservatively. The type of treatment had no effect on the occurrence of cardiovascular events .
| null |
163_biomrc
|
Title: Markers of @entity1593 and XXXX in @entity162 .
OBJECTIVES: Increased immune sensitivity to dietary gluten proteins has been reported in Participants, persons61 but has not been studied in Participants, persons62 . In this study, we examine the levels of antibody reactivity to gliadin, deamidated gliadin, and tissue transglutaminase, tTG ( tissue transglutaminase, tTG ) in individuals with Participants, persons62 and compare these levels to those in individuals who do not have any history of Participants, persons46 . METHODS: The sample of 275 individuals included 102 with Participants, persons62 and 173 controls without a Participants, persons46 . Immunoglobulin G (IgG) and immunoglobulin A (IgA) antibodies to gliadin and tissue transglutaminase, tTG and IgG antibodies to deamidated gliadin were measured by enzyme immunoassay. Participants, persons ' levels of antibodies to deamidated gliadin and tissue transglutaminase, tTG were classified based on the cutoffs for positivity that are predictive of Participants, persons593 . Quantitative levels of antibodies were compared between groups employing regression models which were controlled for demographic variables. RESULTS: Individuals with Participants, persons62 had increased levels of IgG antibodies to gliadin compared with controls in multivariate analyses. We also found evidence of increased levels of antibodies to deamidated gliadin in the Participants, persons62 population. The levels of IgA class antigliadin antibodies and antibodies to tissue transglutaminase, tTG did not differ significantly between groups. There was also not a significant difference between groups in the number of Participants, persons who were classified as having levels of antibodies to deamidated gliadin or tissue transglutaminase, tTG that are predictive of Participants, persons593 . CONCLUSIONS: Individuals with Participants, persons62 have increased levels of IgG antibodies to gliadin. However, such antibody increase is not accompanied by an elevation in IgA antibodies to gliadin or the Participants, persons593 -associated antibodies against deamidated gliadin and tissue transglutaminase, tTG . These results warrant further detailed examination of the molecular specificity and pattern of reactivity of the antibody response to gluten antigens in Participants, persons62 .
| null |
166_biomrc
|
Title: Use of XXXX and @entity2340 fluorescence as probes in kinetic analyses of @entity10840 .
Although the use of 2-aminopurine ( 2-AP ) as a probe in stopped-flow analyses of DNA polymerase beta, Pol beta ( DNA polymerase beta, Pol beta ) had provided important mechanistic insight, the conditions used were limited by the location of 2-AP and the use of a combination of tryptophan (Trp) and 2-AP fluorescence. This study examined different DNA substrates to identify several factors that can affect the observed signal in stopped-flow experiments. Both Trp and 2-AP emissions were separately excited and monitored. It was found that both probes show a fast phase and a slow phase of fluorescence changes, but the direction and the amplitude vary greatly between the two probes and between different DNA substrates. Detailed analyses suggested that the location of 2-AP in the template has a significant impact on the fluorescence properties of 2-AP and that a location opposite the incoming dNTP , which has been used in all such studies in the past, is not optimal. In particular, the results show that placing 2-AP one base after the templating base greatly enhances the signal intensity, which suggests a significant change in base stacking interactions at this position during nucleotide incorporation. These results allowed us to derive an improved set of conditions which were then used to reevaluate results from previous reports. It also allows greater freedom in the type of base pairs studied, since 2-AP is not the templating base in the nascent base pair. Kinetic constants were determined for dNTP and catalytic Mg(2+) . The results obtained from stopped-flow experiments were compared to results from chemical quench. Stopped flow of incorrect dNTP incorporation and the reverse reaction are also reported, which provide useful information to the mechanism of DNA polymerase beta, Pol beta .
| null |
4265_biomrc
|
Title: Whole-body reaching as a measure of dynamic balance in @entity1 with XXXX .
OBJECTIVES: To investigate whether whole-body reaching (WBR) could be a task with adequate and gradable dynamic balance challenges for stroke patients . DESIGN: A total of 23 subjects with patients158 , after their first stroke , participated in this study. The degree of dynamic balance challenges of WBR was graded by varying the distance to the targets that the subjects had to reach. A foot-pressure measurement system was used to measure the amount of center-of-pressure shift during performance of WBR and sit-to-stand transfer. The BBS, Berg Balance Scale ( BBS, Berg Balance Scale ) was administered, and the BBS, Berg Balance Scale total score was calculated. Static and dynamic BBS, Berg Balance Scale subscores were separately calculated to further understanding of the subject performance regarding balance. Paired t tests were used to examine the effects of target distance on center-of-pressure shift. Correlations between measures of center-of-pressure shift during performance of WBR and of sit-to-stand transfer, BBS, Berg Balance Scale total score, BBS, Berg Balance Scale static subscores, and BBS, Berg Balance Scale dynamic subscores and measures of center-of-pressure shift during performance of sit-to-stand transfer were analyzed. RESULTS: Significant effects of distance on three of the variables representing amount of center-of-pressure shift were found (P < 0.05). The correlations between WBR and sit-to-stand transfer were positive and moderate. The correlations between WBR and sit-to-stand transfer were stronger when the subjects were reaching for a closer target than when reaching for a farther target. The correlations between WBR and BBS, Berg Balance Scale total score were weak to moderate; the correlations between WBR and three scores of the BBS, Berg Balance Scale across target distance were inconsistent; and the correlations were higher when the subjects were reaching for a farther target than when reaching for a closer target. CONCLUSIONS: WBR for targets at different distances imposed graded dynamic balance challenges on stroke patients and might be an appropriate dynamic balance measure. However, WBR, as applied in this study, showed limitations in measuring the lateral dynamic stability of stroke patients .
| null |
169_biomrc
|
Title: XXXX of the descending thoracic aorta: three hundred sixty-six consecutive cases resected without paraplegia.
PURPOSE: The aim of this study was to present a 20-year experience with a single method of passive distal perfusion during descending thoracic aortic aneurysms, aneurysm resection. METHODS: Aortic repair with a Dacron graft interposition was performed for 366 consecutive aneurysms, aneurysm located between the left subclavian artery and the crux of the diaphragm. The extent of aorta resected in 335 patients (91.5%) represented one third or less of the aortic length. A 9 mm Gott shunt was cannulated proximally into the ascending aorta (235 cases), the aortic arch (60 cases), the descending aorta (68 cases), or the left ventricle (3 cases) and inserted distally into the descending aorta (232 cases), the femoral artery (127 cases), or the abdominal aorta (7 cases). Shunt flows were recorded in 91 cases and varied from 1100 ml to 4900 ml/min, (mean 2526 ml/min). Distal pressure during shunting was measured in 62 patients . It varied from 15 to 120 mm Hg (mean 64.5 mm Hg). The aortic cross-clamp time varied from 8 to 124 minutes (mean 30 minutes). RESULTS: The hospital death rate was 12% overall and 9.9% (35/351) if patients756 are excluded. Among 359 operating room survivors, neither immediate nor delayed ischemic spinal cord deficit occurred. Transient renal dysfunction occurred in nine patients (2.4%) and patients77 in one (0.2%). Five deaths (1.3%) were shunt related. CONCLUSION: Distal perfusion with the 9 mm Gott shunt has proven to be an effective method to preserve spinal cord function. The limited extent of aorta resected and the brief aortic cross-clamp time may also be interactive factors of protection.
| null |
172_biomrc
|
Title: Affect of antidepressants on the in vitro differentiation of XXXX bone marrow mesenchymal stem cells into neuronal cells.
BACKGROUND: Bone marrow is a valuable source of mesenchymal stem cells (MSCs) that can be used in regenerative medicine. MSCs are able to differentiate into cells from all three germ layers under specific conditions. The aim of the current work was to study the differentiation of rat MSCs (rMSCs) into neuron-like cells. NEW METHOD: We investigated how the antidepressants imipramine , desipramine , fluoxetine and tianeptine affect the differentiation of rMSCs. Furthermore, we present differentiation cocktails using a cortex astrocyte-conditioned medium (CACM) separately or in conjunction with each of the antidepressants and investigated their additive effect on the efficiency of differentiation. We also observed how various differentiation conditions affect the number of primary dendrites and branching dendrites per cell. RESULTS: Gene expression for an early neuronal marker (b-III-tubulin) and markers that are typical for adult neurons such as Th, Htr2A and Slc6a4 were observed. The Tubb3 and Htr2A gene expression were up-regulated, Th decreased slightly and Slc6a4 was down-regulated after differentiation We observed a two-fold higher percentage of b-III-tubulin positive cells after treatment with antidepressants and two-fold increase of neuron-like cells after using CACM with imipramine or fluoxetine simultaneously. Differentiation using imipramine or in conjunction with CACM and desipramine or fluoxetine simultaneously increased the number of cell dendrites. COMPARISON WITH EXISTING METHODS: The results that were obtained are completely new and need further investigations in the nearest future. CONCLUSIONS: These results suggest that antidepressants improve differentiation efficiency of rMSCs and may be useful in the preparation of rMSCs for transplantation. Differentiation efficiency is higher after long-term exposure to antidepressants, than after a 24-h exposure. Nearly additive effect of CACM and imipramine or fluoxetine suggests a beneficial role of antidepressants after transplantation.
| null |
4270_biomrc
|
Title: Effect of @entity5101 on accelerated rejection in XXXX heart transplantation.
The effect of 15-deoxyspergualin ( DSG ) on accelerated rejection was evaluated using a rat, Norway rats, rats heart transplantation model. Lewis rat, Norway rats, rats (LEW, RT1l) served as the organ recipient and Brown rat, Norway rats, rats (BN, RT1n) as the donor. In the accelerated rejection model, the LEW recipient was sensitized with BN skin and BN heart was transplanted 7 days later; the heart graft was rejected within 2 days (n = 7). Histologically, the graft showed coagulation necrosis and hemorrhage throughout the myocardium. DSG (2.5 mg/kg/day) was administered to the recipient under the following three protocols: group 1: during the sensitization period (7 days); group 2: from 3 days after the sensitization to 2 days after grafting (7 days), and group 3: immediately after heart transplantation. The mean graft survival period in groups 1, 2, and 3 was 4.3 +/- 0.8 days (n = 7, p < 0.01 vs untreated host), 11.7 +/- 2.1 days (n = 7, p < 0.001, vs. untreated host), and 2.0 +/- 0 days (n = 6), respectively. The rejected grafts in groups 1 and 3 histologically showed coagulation necrosis and hemorrhage . By contrast, in group 2, the major histological change was interstitial lymphocyte infiltration and there were few findings such as coagulation necrosis or hemorrhage . In the complement-dependent cytotoxicity test, serum obtained at the second posttransplant day from the recipients treated in group 1 showed a high cytotoxicity level, although the cytotoxicity level of serum obtained from the recipients treated in group 2 was consistently low.(ABSTRACT TRUNCATED AT 250 WORDS)
| null |
4271_biomrc
|
Title: [Prescription of a XXXX growth factor, lenograstim, in current practice. Results and commentaries of a national survey from April 1995 to March 1996].
OBJECTIVES: The French drug authorities audited first-time prescription of patients, patient583 growth factors using a prescription follow-up survey. Data collected between April 1995 and March 1996 were analyzed then criticized by three clinical experts working in three different areas where lenograstime is most widely used. METHODS: First-time prescription data and follow-up information were recorded on separate inclusion and follow-up diaries by the prescribing physicians. The delivering pharmacies complete the diaries and addressed them to the INSERM unit 330 for analysis. RESULTS: There were 7,102 inclusion diaries and 1376 follow-up diaries from 234 different hospital facilities. Lenograstime was most frequently prescribed in patients, patient with lymphoma (19%), breast cancer (16.4%), and lung cancer (13.8%). Prescriptions involved 377 different chemotherapy protocols, including 196 which concerned a single patients, patient . At the first prescription, lenograstime was given as a preventive measure in 61% of the cases and for curative therapy in 25.3%. The planned duration of preventive treatment was longer than the true period of treatment. DISCUSSION: Pr Rossi, hematologist, Pr Misset, cancerologist and Pr Lebeau, pneumologist criticized the findings.
| null |
2225_biomrc
|
Title: XXXX GENE POLYMORPHISM MODULATES THE RISK OF PERIODONTAL DISEASES.
gingivitis, Gingivitis and periodontitis are the two main Periodontal diseases, periodontal diseases . Both are characterized by inflammation of the tissues surrounding the teeth but while tissue damages observed in gingivitis, Gingivitis are mild and reversible, destruction caused by periodontitis is deeper and irreversible. Periodontal diseases, periodontal diseases and levels of degeneration of tissues surrounding teeth depend on several interacting endogenous and exogenous factors. Polymorphisms of genes encoding molecules that modulate the immune response and tissue homeostasis are the main causes of individual susceptibility to Periodontal diseases, periodontal diseases . The aim of this study was to investigate IL6 , IL10 and patients7894 gene polymorphisms in a large number of subjects affected by either gingivitis, Gingivitis or chronic periodontitis . The sample included 750 Italian patients . We found that the patients5147 SNP located in the IL6 gene promoter was strongly associated with the occurrence of both gingivitis, Gingivitis and periodontitis . Indeed, homozygous individuals with variant allele appeared less-susceptible to both gingivitis, Gingivitis OR=0.47 (95% C.I. 0.27-0.82) and periodontitis OR=0.36 (95% C.I. 0.21-0.64). No evidence of association between Periodontal diseases, periodontal diseases and IL10 or patients7894 polymorphisms was obtained. This data confirmed the role of IL6 in susceptibility to periodontitis among the Italian population. The evidence that IL6 polymorphisms are also involved in gingivitis, Gingivitis has implications in periodontal disease pathogenesis and reduces the appeal of IL6 as a periodontitis biomarker.
| null |
2230_biomrc
|
Title: @entity479 Use and Infectious Complication in Peritoneal Dialysis After XXXX .
BACKGROUND: Peritoneal dialysis, PD, peritonitis ( Peritoneal dialysis, PD, peritonitis ) is one potential treatment option for patients starting dialysis after graft loss (DAGL). However, the infectious outcomes and their associations with steroid use remain undetermined in these patients . METHODS: A total of 41 DAGL patients undergoing Peritoneal dialysis, PD, peritonitis were recruited. The patients were divided into low- and high-dose steroid groups according to the median level. Additionally, they were categorized into tapering and nontapering groups, for which the tapering regimen was defined as the withdrawal of steroids within 1 year after starting Peritoneal dialysis, PD, peritonitis . Primary outcomes, such as Peritoneal dialysis, PD, peritonitis and exit site infection (ESI), were compared between DAGL and 712 TN, transplant-naive ( TN, transplant-naive ) patients . RESULTS: The overall risk of Peritoneal dialysis, PD, peritonitis was similar between the TN, transplant-naive and DAGL groups. However, when the DAGL group was stratified by the steroid variable, the risk was higher in the high-dose or nontapering steroid groups than in the TN, transplant-naive or counterpart steroid groups. The DAGL group had a higher risk of ESI than the TN, transplant-naive group, irrespective of steroid dose. When the analysis was stratified by tapering regimen, the difference in ESI risks was seen only in the nontapering group and the TN, transplant-naive group; the tapering group had a similar risk of ESI as that of the TN, transplant-naive group. CONCLUSIONS: The present study first addresses the risks of Peritoneal dialysis, PD, peritonitis and ESI together and then raises awareness of the high risk that should be considered after using high-dose steroids or the nontapering regimen in the DAGL patients .
| null |
2231_biomrc
|
Title: An enzymatic assay for XXXX concentration in human serum and plasma.
OBJECTIVES: Several methods for measuring lysophosphatidylcholine ( LPC ) concentrations have been reported. However, these methods are not practical because they are either too complicated and/or too time-consuming for LPC determinations in human serum and plasma. DESIGN AND METHODS: We have developed a new enzymatic LPC assay, which uses lysophospholipase, glycerophosphorylcholine phosphodiesterase and choline oxidase, and which determines the quantities of hydrogen peroxide generated in the presence of peroxidase using an oxidative chromogenic reagent and 4-aminoantipyrine . RESULTS: Various samples were mixed with LPC assay reagents, and their changes in absorbance were measured. The present method produced a linear calibration line between LPC concentration and absorbance change. It also measured only LPC , and not other phospholipids such as phosphatidylcholine , sphingomyelin and lysophosphatidic acid . The within-run and between-run coefficients of variation were 0.3-0.7% and 0.7%, respectively. The recovery of exogenous LPC added to control serum was 99.5-102.1%. The correlation coefficient obtained in a comparison with a method for analyzing fatty acids was 0.9122. CONCLUSIONS: The present method is simple, specific for LPC , and can be applied with an automatic analyzer. It may also be useful for further studies of the biological functions of LPC as well as clinical applications in various disorders.
| null |
4280_biomrc
|
Title: Single-stage debridement and spinal fusion using XXXX cages through a posterior approach for eradication of lumbar pyogenic @entity1512 : a safe treatment strategy for a detrimental condition.
BACKGROUND: Pyogenic infections of the lumbar spine are a rare but critical pathology, yet with considerably high mortality rates. In cases indicating surgical therapy, the implantation of titanium cages or autologous bone grafts represent today's gold standard. Although non-metallic implants such as Patients, patients, patient2316 ( Patients, patients, patient2316 ) have proven to be advantageous in diverse degenerative conditions, their saftey and practicability in lumbar spine infection remains questionable. Moreover, the efficacy of a single-step radical debridement of the infected disc space with subsequent fusion from a strictly posterior approach continues to be an issue of debate. We therefore sought to evaluate the feasibility, clinical and radiological outcome of a single-step TLIF procedure using oblique Patients, patients, patient2316 cages in the surgical management of Patients, patients, patient with lumbar pyogenic Patients, patients, patient512 . METHODS: From January 2009 through December 2013, all Patients, patients, patient meeting the indication for surgical treatment of lumbar pyogenic Patients, patients, patient512 were included. Patients, patients, patient demonstrating intact cortical bone on preoperative CT received a single-step radical debridement of the infected intervertebral disc space, posterior screw-and-rod instrumentation and implantation of an oblique Patients, patients, patient2316 cage using the TLIF technique. Oral antibiotics were continued for 12 weeks postoperatively and clinical and radiological results recorded throughout a minimum 1-year clinical follow-up. RESULTS: A total of 104 Patients, patients, patient were admitted to receive surgical therapy for lumbar pyogenic Patients, patients, patient512 . Within this Patients, patients, patient population, 18 Patients, patients, patient met the diagnostic criteria to receive the implantation of an oblique Patients, patients, patient2316 cage. Pathogens were detected in 13 cases with Staph. aureus being the predominant causative organism. All Patients, patients, patient were discharged to recover in their domestic environment. Throughout the first year of clinical and radiological follow-up and beyond, none of the 18 Patients, patients, patient demonstrated any signs of residual neurologic deficits or recurrent infection. Furthermore, two-plane conventional X-rays showed no significant implant subsidence or failure at any of the given time-points in up to 5 years postoperatively. CONCLUSIONS: In Patients, patients, patient meeting the criteria for surgical treatment of lumbar pyogenic Patients, patients, patient512 , the implantation of Patients, patients, patient2316 cages using a single-step TLIF approach is a safe and feasible procedure. Based on our experience, the concern of a recurrent infection when implanting non-metallic cages may be refuted in carefully selected Patients, patients, patient .
| null |
186_biomrc
|
Title: Endotoxin tolerance of adrenal gland: attenuation of XXXX production in response to lipopolysaccharide and @entity4545 .
OBJECTIVES: Reversible patients887 frequently has been diagnosed in critically ill patients with sepsis who have either low basal cortisol levels or low cortisol responses to adrenocorticotropic hormone (ACTH) stimulation. It is generally accepted that a phenomenon called "endotoxin tolerance" contributes to immunosuppression during sepsis . The present study was to investigate whether endotoxin tolerance occurs in the adrenal gland, leading to hyporesponsiveness of adrenal gland during sepsis . DESIGN: Controlled laboratory experiment. SETTING: University research laboratory. SUBJECTS: Sprague-Dawley male Rats, rats 200-250 g and primary isolated adrenal patients2288 cells. INTERVENTIONS: Rats, rats received intra-arterial injection of purified lipopolysaccharide (0.5 mg/kg) through indwelling femoral arterial catheters, and 24 hrs later the adrenocortical sensitivity to exogenous ACTH (10 ng/kg) was detected. Primary patients2288 cells were pretreated with lipopolysaccharide at 0.1-100 ng/mL or with ACTH at 0.01-10 ng/mL and then challenged, in fresh media, with 1 g/mL lipopolysaccharide or 10 ng/mL ACTH. MEASUREMENTS AND MAIN RESULTS: Toll-like receptor 4 was expressed in adrenal gland and primary patients2288 cells. Plasma corticosterone response to ACTH was decreased in Rats, rats receiving preinjection of lipopolysaccharide. Lipopolysaccharide pretreatment caused a significant decrease in corticosterone production in response to subsequent ACTH and lipopolysaccharide stimulation in primary patients2288 cells. patients7296 pretreatment inhibited ACTH- and lipopolysaccharide-induced expression of patients34 metabolizing enzymes. Lipopolysaccharide significantly decreased Toll-like receptor 4 and ACTH receptor expression. CONCLUSIONS: Pre-exposure to lipopolysaccharide resulted in hyporesponsiveness to ACTH stimulation in Rats, rats . In vitro, patients7296 pretreatment impaired corticosterone production of patients2288 cells in response to ACTH and lipopolysaccharide, which was associated with decreased expression of synthetic enzymes required for corticosterone production. Our results indicate that endotoxin tolerance of adrenal gland is one of the mechanisms for adrenocortical insufficiency during sepsis .
| null |
189_biomrc
|
Title: A quantitative study of XXXX deposition in the aortic wall following Medtronic Freestyle compared with homograft aortic root replacement. A prospective randomized trial.
BACKGROUND AND AIM OF THE STUDY: Unstented aortic valve substitutes offer many of the theoretical advantages of homografts such as superior hemodynamic performance and enhanced durability, particularly when inserted as a root. Many of these depend on the maintained flexibility of the valve components. Calcification of the aortic wall may adversely affect these phenomena. Electron beam computed tomography has been used to evaluate aortic wall calcification in patients, Patients undergoing aortic root replacement in a prospective randomized trial designed to compare the Medtronic Freestyle valve with the homograft valve replacement. METHODS: patients, Patients were followed with electron beam computed tomography scans of the aortic root at six-monthly intervals after surgery. A calcification score (Hounsfield units) and a calcified volume score (mm3) were obtained from each scan using a new modified technique. Results were related to hemodynamic data from echocardiography. The prevalence of calcification was also related to the homograft donor age. RESULTS: Seventy-six patients, Patients (age range: 40-79 years) were randomized to root replacement with either homograft (n = 31) or Freestyle (n = 45) valves. Fifty-three scans of the aortic root were performed postoperatively in 37 patients, Patients . No statistical difference between the two groups was found at six and 12 months after surgery. However, after 18 months the calcified volume score was 5903.8+/-2356.8 mm3 in the homograft versus 2725.6+/-1500.5 mm3 in the Freestyle group (p = 0.017). There was a correlation between calcification score, calcified volume score and left ventricular mass (r = 0.323, p = 0.093 and r = 0.350, p = 0.068, respectively) on the one hand, and calcification score, calcified volume score and valve size on the other hand (r = 0.178, p = 0.466 and r = 0.068, p = 0.780, respectively). CONCLUSIONS: Electron beam computed tomography provides a powerful tool for the detection of calcium in the aortic wall of valve grafts. There is a low rate of calcification during the first 18 months in the Medtronic Freestyle valve, and this appears to be lower than that observed in homografts. Longer-term follow up of the aortic root in these patients, Patients is required. This is an ongoing study.
| null |
4516_biomrc
|
Title: Whole Genome Sequencing in Real-Time Investigation and Management of a XXXX Outbreak on a Neonatal Intensive Care Unit.
UNASSIGNED: OBJECTIVE To use whole genome sequencing to describe the likely origin of an outbreak of Pseudomonas aeruginosa, P. aeruginosa in a neonatal unit. DESIGN Outbreak investigation. SETTING The neonatal intensive care unit service of a major obstetric tertiary referral center. patient, PATIENTS, infants, Infants patient, PATIENTS, infants, Infants admitted to the neonatal unit who developed Pseudomonas aeruginosa, P. aeruginosa colonization or infection . METHODS We undertook whole genome sequencing of Pseudomonas aeruginosa, P. aeruginosa strains isolated from colonized patient, PATIENTS, infants, Infants and from the neonatal unit environment. RESULTS Eighteen patient, PATIENTS, infants, Infants were colonized with Pseudomonas aeruginosa, P. aeruginosa . Isolates from 12 patient, PATIENTS, infants, Infants and 7 environmental samples were sequenced. All but one of the clinical isolates clustered in ST253 and no differences were detected between unmapped reads. The environmental isolates revealed a variety of sequence types, indicating a large diverse bioburden within the unit, which was subsequently confirmed via enterobacterial repetitive intergenic consensus polymerase chain reaction typing of post-outbreak isolates. One environmental isolate, obtained from a sink in the unit, clustered within ST253 and differed from the outbreak strain by 9 single-nucleotide polymorphisms only. This information allowed us to focus infection control activities on this sink. CONCLUSIONS Whole genome sequencing can provide detailed information in a clinically relevant time frame to aid management of outbreaks in critical patient, PATIENTS, infants, Infants management areas. The superior discriminatory power of this method makes it a powerful tool in infection control. Infect Control Hosp Epidemiol 2015;00(0):1 7.
| null |
195_biomrc
|
Title: Motility of the small intestine after proctocolectomy and @entity4628 -anal XXXX .
Though the mechanisms of continence after proctocolectomy and ileal pouch -anal anastomosis have been studied, functions of the small intestine have received little attention. However, frequent stools and urgency plague some patients who are otherwise quite continent. Motility of the jejunum and ileum was assessed in eight patients with ulcerative colitis who were studied 4 to 24 months after proctocolectomy and ileal pouch -anal anastomosis ; these findings were compared to those in six healthy volunteers. Continuous manometric recordings from the small bowel were obtained in both groups for 16 to 23 hours of fasting ; postprandial recordings were made for 6 hours following a mixed meal (800 kcal, 20% protein, 40% fat, 40% patients735 ) in the ileoanal patients . The duration, velocity of propagation, and periodicity of the migrating motor complex did not differ between the groups (P greater than 0.05). Discrete bursts of clustered contractions were recorded from all of the controls and in five of eight patients . Likewise, we recorded from all controls and five of eight patients large amplitude, prolonged waves of pressure which propagated distally. However, in controls these large amplitude waves were confined to the terminal ileum, but in patients these were detected in the jejunoileum, up to 125 cm proximal to the ileal pouch . We conclude that jejunoileal motility is not greatly altered by proctocolectomy with ileal pouch -anal anastomosis . However, the appearance of the large amplitude, rapidly propagating waves in the proximal jejunoileum after operation may be a response to increased storage within and distention of the distal bowel.
| null |
4292_biomrc
|
Title: Tissue-specific transcriptomics and proteomics of a filarial XXXX and its Wolbachia endosymbiont.
BACKGROUND: Filarial nematode, nematodes cause debilitating human diseases. While treatable, recent evidence suggests drug resistance is developing, necessitating the development of novel targets and new treatment options. Although transcriptomic and proteomic studies around the nematode, nematodes life cycle have greatly enhanced our knowledge, whole organism approaches have not provided spatial resolution of gene expression, which can be gained by examining individual tissues. Generally, due to their small size, tissue dissection of human -infecting filarial nematode, nematodes remains extremely challenging. However, canine heartworm disease is caused by a closely related and much larger filarial nematode, nematodes , D. immitis, Dirofilaria immitis . As with many other filarial nematode, nematodes , D. immitis, Dirofilaria immitis contains Wolbachia, an obligate bacterial endosymbiont present in the hypodermis and developing oocytes within the uterus. Here, we describe the first concurrent tissue-specific transcriptomic and proteomic profiling of a filarial nematode, nematodes ( D. immitis, Dirofilaria immitis ) and its Wolbachia (wDi) in order to better understand tissue functions and identify tissue-specific antigens that may be used for the development of new diagnostic and therapeutic tools. METHODS: Adult D. immitis, Dirofilaria immitis worms were dissected into female body wall (FBW), female uterus (FU), human944 ( human944 ), human35 ( human35 ), male body wall (MBW), male testis (MT), human0552 ( human0552 ), MH, male head ( MH, male head ) and 10.1186/s12864-015-2083-2 male spicule (MS) and used to prepare transcriptomic and proteomic libraries. RESULTS: Transcriptomic and proteomic analysis of several D. immitis, Dirofilaria immitis tissues identified many biological functions enriched within certain tissues. Hierarchical clustering of the D. immitis, Dirofilaria immitis tissue transcriptomes, along with the recently published whole-worm adult male and female D. immitis, Dirofilaria immitis transcriptomes, revealed that the whole-worm transcriptome is typically dominated by transcripts originating from reproductive tissue. The uterus appeared to have the most variable transcriptome, possibly due to age. Although many functions are shared between the reproductive tissues, the most significant differences in gene expression were observed between the uterus and testis. Interestingly, wDi gene expression in the male and female body wall is fairly similar, yet slightly different to that of Wolbachia gene expression in the uterus. Proteomic methods verified 32 % of the predicted D. immitis, Dirofilaria immitis proteome, including over 700 hypothetical proteins of D. immitis, Dirofilaria immitis . Of note, hypothetical proteins were among some of the most abundant Wolbachia proteins identified, which may fulfill some important yet still uncharacterized biological function. CONCLUSIONS: The spatial resolution gained from this parallel transcriptomic and proteomic analysis adds to our understanding of filarial biology and serves as a resource with which to develop future therapeutic strategies against filarial nematode, nematodes and their Wolbachia endosymbionts.
| null |
2244_biomrc
|
Title: Spreading of XXXX neutrophils is immediately preceded by a large increase in cytoplasmic free @entity460 .
When human polymorphonuclear leukocytes (PMN) are placed on various surfaces, they attach and spread rapidly, increasing their diameter severalfold. The spreading is associated with extensive changes in the cytoskeleton. Since many cytoskeletal events are regulated by human41 , we measured the cytosolic free calcium concentration ([ human41 ]i) in individual human PMN as they spread. [ human41 ]i was measured in single cells by microspectrofluorometry using the fluorescent human41 -sensitive dye fura-2 . Immediately before spreading, PMN exhibit a rapid increase in [ human41 ]i, from 69 +/- 51 nM to 547 +/- 190 nM (mean +/- SD, n = 12). [ human41 ]i returns to near resting levels during the next minute, as the cells spread. Neither the spreading nor the [ human41 ]i spike is blocked by removal of extracellular calcium , by human855 , by calmodulin antagonists, or by mitochondrial or microtubule poisons. Spreading, but not the [ human41 ]i increase, is blocked by the microfilament inhibitor cytochalasin B. Both spreading and the [ human41 ]i spike are blocked by ATP depletion and reversibly blocked by placing the cells in medium containing hypertonic sucrose or sodium chloride . These data strongly suggest that an increase in [ human41 ]i, derived from nonmitochondrial intracellular pools, plays an important role in the microfilament-mediated process of PMN spreading.
| null |
2247_biomrc
|
Title: XXXX and autoantibody fixation in an experimental model of @entity121 .
An experimental model of autoimmune myopathy, soleus muscle lesions, muscle damage was designed using parental antigens (muscle mitochondrial fraction) in F1 hybrid W rats, Wistar, rats (male W rats, Wistar, rats x female Sprague-Dawley). The immune response was modulated by spleen fragment transplant from either W rats, Wistar, rats (W) or F1. Antibody fixation and inflammatory reaction were studied in Extensor digitorum longus and soleus muscles. Immunization without spleen transplant resulted in antibody fixation mainly in capillaries and incompletely around muscle fibers; whorled fibers were found in 1/3 of F1 W rats, Wistar, rats immunized with antigen from W rats, Wistar, rats . Spleen transplants from Sprague Dawley (SD) W rats, Wistar, rats were usually accepted by F1; in some animals, antibodies surrounded completely muscle fibers and the percentage of animals showing autoimmune myopathy, soleus muscle lesions, muscle damage was increased. Spleen transplants from non immunized F1 were usually rejected by immunized F1; antibody reaction was found inside fibers of most of the W rats, Wistar, rats , autoimmune myopathy, soleus muscle lesions, muscle damage was present in 40% of the animals immunized with W, but absent in those immunized with SD antigen. In conclusion, this model can be used to study immunological responses to alloantigens (parental to F1). Spleen fragment transplant modulates the immune response. There was discrepancy between antibody fixation and autoimmune myopathy, soleus muscle lesions, muscle damage . The immunological response was different according to muscle fiber type composition and/or microcirculatory characteristics.
| null |
2249_biomrc
|
Title: [Clinical and molecular diagnosis of XXXX ].
OBJECTIVE: The aim of this work was to pinpoint familial breast and/or ovarian cancer, breast and/or ovarian cancer, inherited breast-ovarian cancer risk. Clinical cancer genetics include: diagnostic cancer genetics, cancer genetic counseling and management of women, patients, people, men at high risk of developing familial breast and/or ovarian cancer, breast and/or ovarian cancer, inherited breast-ovarian cancer . MATERIAL AND METHODS: An update of documented data was performed in order to assess our current knowledge about familial breast and/or ovarian cancer, breast and/or ovarian cancer, inherited breast-ovarian cancer . RESULTS: Most breast cancers, BC ( breast cancers, BC ) are sporadic while 5-10% are estimated to be due to an inherited predisposition. Rare autosomal dominant alteration in two genes, cancer345 and BRCA2 , which confer a high risk of developing BC and ovarian cancer (OC), are likely to account for most families with multiple cases of early-onset breast cancers, BC and OC, but only 3-4% of all breast cancers, BC . The estimations of their prevalence and penetrance vary greatly, depending on the population studied, the study design, statistical methods and the sensitivity of technical methods for detection of mutations. Penetrance can be very high, but incomplete (maximum 80%). Penetrance and age at onset of the same mutation show great variability within and between breast cancers, BC families. This could be due to the effects of other genetic as well as non-genetic factors that are being studied. The heterogeneity of families makes the identification of other high-risk genes difficult. Additive effects of several susceptibility genes could explain much more breast cancers, BC in the general population (polygenic models). Molecular diagnoses have become feasible. Specific consultations in oncogenetics help clinicians and women, patients, people, men understand hereditary components, establish molecular diagnoses, provide guidelines for a better surveillance of women, patients, people, men at high risk of developing breast cancers, BC , OC or both and reassure the mutation non-carriers. The strategies of management and treatment of breast cancers, BC and OC in mutation carriers remain under discussion and it is is difficult to choose preventive options. CONCLUSION: Inherited breast cancers, BC or OC due to a mutation in cancer345 or BRCA2 are rare in the general population. However, women, patients, people, men and women, patients, people, men have the right to be informed of the possibilities concerning presymptomatic testing, risk assessment, surveillance, prevention and psychological support with respect to the potential benefits and limitations. Other large prospective studies are needed to validate rapidly the methods of surveillance and prevention in this group at "high risk" in order to adapt them to very large populations. However, predictive medicine should be used with care.
| null |
2250_biomrc
|
Title: Chronic cerebroventricular galanin does not induce sustained XXXX or obesity.
Acute central administration of galanin has been reported to increase fat consumption. These experiments were designed to test the hypothesis that repeated injections of galanin would elicit hyperphagia and weight gain and that this response would depend on the available diet. Male Sprague-Dawley rats, rats were fed high (56% energy) or low (10% energy) fat diets. Galanin (300 pmol) or saline vehicle was injected into the third ventricle twice daily for 7 days and three times daily for another 6 days. On both the high- carbohydrate and high-fat diets, twice daily galanin increased daytime food intake, but there was a compensatory decrease in nighttime intake. The addition of a third, nighttime injection of galanin was ineffective in producing an increase in total 24-h intake. There was no significant increase in body weight during galanin treatment in Sprague-Dawley rats, rats eating either diet although animals eating the high-fat diet gained more weight as reflected by a significant increase in epididymal fat pad weight. Galanin treatment had no effects on serum insulin, glucose or corticosterone concentrations, measured at the end of the experiment. However, animals fed the high-fat diet had significantly higher insulin concentrations at the time of sacrifice. Although repeated central infusions of galanin reliably stimulated daytime intake of both diets, they failed to increase total daily energy intake or body weight.
| null |
203_biomrc
|
Title: Identifying Loci Contributing to Natural Variation in Xenobiotic Resistance in XXXX .
Natural populations exhibit a great deal of interindividual genetic variation in the response to toxins, exemplified by the variable clinical efficacy of pharmaceutical drugs in humans, human , and the evolution of pesticide resistant insects. Such variation can result from several phenomena, including variable metabolic detoxification of the xenobiotic, and differential sensitivity of the molecular target of the toxin. Our goal is to genetically dissect variation in the response to xenobiotics, and characterize naturally-segregating polymorphisms that modulate humans, human37 . Here, we use the Drosophila Synthetic Population Resource (DSPR), a multiparent advanced intercross panel of recombinant inbred lines, to identify QTL (Quantitative Trait Loci) underlying xenobiotic resistance, and employ caffeine as a model toxic compound. Phenotyping over 1,700 genotypes led to the identification of ten QTL, each explaining 4.5-14.4% of the broad-sense heritability for caffeine resistance. Four QTL harbor members of the cytochrome P450 family of detoxification enzymes, which represent strong a priori candidate genes. The case is especially strong for Cyp12d1 , with multiple lines of evidence indicating the gene causally impacts caffeine resistance. Cyp12d1 is implicated by QTL mapped in both panels of DSPR RILs, is significantly upregulated in the presence of caffeine , and RNAi knockdown robustly decreases caffeine tolerance. Furthermore, copy number variation at Cyp12d1 is strongly associated with phenotype in the DSPR, with a trend in the same direction observed in the DGRP ( Drosophila Genetic Reference Panel). No additional plausible causative polymorphisms were observed in a full genomewide association study in the DGRP, or in analyses restricted to QTL regions mapped in the DSPR. Just as in humans, human populations, replicating modest-effect, naturally-segregating causative variants in an association study framework in flies will likely require very large sample sizes.
| null |
2252_biomrc
|
Title: Six-year follow-up of laser in situ keratomileusis for moderate and extreme XXXX using a first-generation excimer laser and microkeratome.
PURPOSE: To evaluate objectively and subjectively the long-term outcome of laser in situ keratomileusis (LASIK) in patients with high and very high patients714 . SETTING: Department of Ophthalmology, Philipps University, Marburg, Germany. METHODS: Thirty-three eyes of 19 patients were followed for a mean of 76 months (range 50 to 84 months) after primary LASIK using the Keratom I excimer laser (Schwind) and the ALK microkeratome (Chiron). Refraction, glare, pachymetry, corneal topography, and tear-film secretion and stability were measured. At the last examination, patients also answered a 14-item questionnaire. RESULTS: Preoperatively, the mean spherical equivalent was -13.65 diopters (D). At 1 year, it was -0.25 D and after 6 years, -0.88 D. Fifteen percent of eyes lost > or =2 lines of best spectacle-corrected visual acuity (BSCVA), and 9% gained > or =2 Snellen lines. At the end of the study, 46% of eyes were within +/-1.0 D of the attempted corrected and 88% were within +/-3.0 D. There were 5 microkeratome-associated complications; 3 resulted in loss of BSCVA. The latest pachymetry showed a mean corneal thickness of 498.5 microm (range 396 to 552 microm). There were no cases of keratectasia. Seventy-five percent of patients noted an increase in their quality of life. Seventy-one percent were satisfied with their postoperative visual acuity; however, 75% noticed glare and halos at night. CONCLUSIONS: Laser in situ keratomileusis correction of very high patients714 did not cause keratectasia in the long term provided the corneal thickness was respected. A flap thickness setting of 130 microm with a first-generation microkeratome resulted in a high number of cut failures. Most patients were happy with the results despite a modest level of accuracy and glare.
| null |
4306_biomrc
|
Title: Does severe @entity417 caused by dose-dense @entity1311 - @entity3003 combination therapy have an effect on the survival of XXXX with @entity43 ? Retrospective analysis of the Japanese gynecologic oncology group 3016 trial.
INTRODUCTION: To evaluate the incidence of anemia in patients with ovarian cancer, epithelial ovarian cancer receiving patients311 - carboplatin combination therapy ( TC ) using data from the Japanese Gynecologic Oncology Group (JGOG) 3016 trial, and to examine the effect of severe anemia on survival during dose-dense TC . METHODS: Retrospective analysis was conducted in patients enrolled in the JGOG 3016 trial who underwent at least one cycle of the protocol therapy (n = 622). Hemoglobin values at enrollment and during each cycle of TC were collected. One-to-one matching was performed between patients with and patients without grade 3/4 anemia during TC ( anemia and nonanemia groups) to adjust the baseline characteristics of the patients . The cumulative survival curve and median progression-free survival were estimated using the Kaplan-Meier method. RESULTS: Grades 2 to 4 anemia was observed in 19.8% of patients before first-line TC . The incidence of grade 3/4 anemia rapidly increased to 56.1% after the fourth cycle of dose-dense TC . After matching, the median progression-free survival in the anemia (hemoglobin <8.0 g/dL) and nonanemia (hemoglobin >8.0 g/dL) groups was 777 and 1100 days, respectively (P = 0.3493) for patients receiving dose-dense TC . The median progression-free survival in patients receiving conventional TC was similar between the 2 groups. CONCLUSIONS: The difference in progression-free survival between patients with ovarian cancer, epithelial ovarian cancer with and those without severe anemia during TC was not statistically significant, but for patients receiving dose-dense TC , severe anemia seems to have prognostic relevance. Prospective trials are needed to investigate whether the optimal management of chemotherapy-induced anemia , including appropriate use of erythropoiesis-stimulating agents, would further improve the survival of patients with ovarian cancer, epithelial ovarian cancer receiving dose-dense TC .
| null |
4307_biomrc
|
Title: Hyper-regulation of pyr gene expression in XXXX cells with slow ribosomes. Evidence for RNA polymerase pausing in vivo?
UTP -modulated attenuation of transcription is involved in regulating the synthesis of pyrimidine nucleotides in Escherichia coli . Thus, expression of two genes, pyrBI and pyrE, was shown to be under this type of control. The genes encode the two subunits of aspartate transcarbamylase and orotate phosphoribosyltransferase respectively. The levels of these enzymes are inversely correlated with the intracellular concentration of UTP . Modulation of attenuation seems to be a consequence of the effect of UTP concentration on the mRNA chain growth rate. Reducing the UTP pool retards RNA polymerase movement. Mechanistically this will couple the ribosomes translating a leader peptide gene more tightly to the elongating RNA polymerase. The ribosomes will then be more prone to prevent the folding of the mRNA chains into terminating hairpin structures when RNA polymerase is at the attenuator and has to decide whether transcription should terminate or continue into the structural genes. This paper described a study of pyrBI and pyrE gene regulation in cells where the ribosomes move slowly as a result of mutation in rpsL. It appears that expression of the two genes is hyper-regulated by the UTP pool in this type of cells. Furthermore, the attenuator model can only account for the results if it is assumed that UTP -concentration-dependent pausing of transcription occurs in vivo in the two pyr gene leaders such that RNA polymerase waits for the coupled ribosomes before transcribing into the attenuator regions.
| null |
4309_biomrc
|
Title: [An elderly @entity1893 XXXX with a massive @entity5 of the heart].
We report on an elderly woman, patient with a lymphoma, Lymphoma, malignant lymphoma forming a huge mass in the heart. An 82-year-old woman, patient became aware of general fatigue and a cough in August 1999. Her right supraclavicular, bilateral axillary, and right inguinal lymph nodes were swollen. A hypodermical mass in the right frontal chest was detected. Her left axillary lymph node was biopsied. She was diagnosed as having woman, patient893 , diffuse large cell type, B-cell type. Computed tomography scans showed a markedly thickened right ventricular wall of the heart, swollen lymph nodes of the mediastinum, bilateral woman, patient143 , and a tumor in the spleen. lymphoma, Lymphoma, malignant lymphoma cells were found in the woman, patient143 , and the lymphoma, Lymphoma, malignant lymphoma was diagnosed as clinical stage IV. woman, patient887 of the heart, ejection fraction (EF) 49%, was demonstrated with transthoracic echocardiography. EF increased to 70% after 3 courses of chemotherapy with CHOP regimen. All lesions disappeared after 6 courses of chemotherapy were completed. After consolidative radiotherapy with a total dose of 37 Gy to the mediastinum and heart, bilateral woman, patient143 , elevation of the woman, patient 's lactate dehydrogenase level and soluble woman, patient199 receptor value were recognized, which suggested relapse of the lymphoma, Lymphoma, malignant lymphoma , although histopathological confirmation could not be realized.
| null |
2263_biomrc
|
Title: [Acute XXXX in the posterior fossa (author's transl)].
A 2-year-old girl was admitted to Chugoku Rosai Hospital one hour after a car accident on July 16, 1978. On admission she was conscious and no physical or girl6 was found. However, 2 hours and a half after the accident, she was attacked suddenly by generalized convulsion , and then became comatose with apnea . Bilateral horizontal nystagmus to the left, rhythmic constriction and dilation of the pupils reacting to light, and flaccid girl688 were recognized. Plain skull films revealed a linear girl74 in the right posterior fossa, which crossed the transverse this region. A burr hole was made in the left posterior fossa, and slightly xanthochromic fluid, 30 to 40 microliter in volume, under high pressure was evacuated from the subdural space. A burr hole on the right side disclosed no abnormality. Spontaneous respiration returned immediately and consciousness became clear 5 hours after the surgery. No neurologic deficit was detect and postoperative EEG was normal. She was discharged from the hospital on July 27, 1978. Ten cases of acute girl1757 in the posterior fossa, including the present case, were analyzed. This lesion is able to be cured completely by a simple operation even in coma with apnea , if diagnosis made promptly and the surgery is carried out without delay. In cases of acute head injury , it is quite important to have a possibility of the infratentorial lesion in mind and to find it with the aids of plain skull film of Towne's view, CT scan, and VAG if necessary.
| null |
215_biomrc
|
Title: Role of apoptosis-inducing factor in myocardial cell XXXX by @entity63 -reperfusion.
Although apoptosis contributes to myocardial cell death in the ischemia, ischemic -reperfused heart, the molecular basis of apoptosis is poorly understood. Apoptosis-inducing factor, AIF ( Apoptosis-inducing factor, AIF ) has been characterized as a caspase-independent death effector. Upon the induction of apoptosis, mitochondrial Apoptosis-inducing factor, AIF is released to the cytoplasm and then enters the nucleus, in which it induces chromatin condensation and 50 kbp DNA fragmentation. In the present study, we examined the role of Apoptosis-inducing factor, AIF in ischemia, ischemic -reperfusion injury in isolated rat hearts. Apoptosis-inducing factor, AIF was detected in the cytosolic and nuclear fractions of hearts subjected to ischemia, ischemic -reperfusion, whereas it was detected only in the mitochondria of control hearts. Moreover, Apoptosis-inducing factor, AIF release increased in a reperfusion time-dependent manner. Pulse field gel electrophoresis revealed that 50 kbp DNA fragments were produced by ischemia, ischemic /reperfusion. In contrast, cytochrome c release and the activation of caspase-3 did not occur to a significant extent. Moreover, ischemia, ischemic preconditioning attenuated the Apoptosis-inducing factor, AIF release and the 50 kbp DNA fragmentation. These results suggest that Apoptosis-inducing factor, AIF -dependent apoptosis is likely to attribute to myocardial cell death in the ischemia, ischemic -reperfused heart and that it is related with the protective effect of ischemia, ischemic preconditioning.
| null |
217_biomrc
|
Title: @entity1824 (so-called epithelioid XXXX ) of the kidney: A clinicopathologic study of 41 cases: detailed assessment of morphology and risk stratification.
metastasis59 ( patients824 ) of the kidney are defined as potentially malignant mesenchymal lesions that are closely related to metastasis59 . Although approximately 120 cases are published, mostly as case reports with variably used diagnostic criteria, the pathologic prognostic predictors of outcome are unknown. We analyzed the clinicopathologic parameters in a large series of 41 cases of pure metastasis59 of the kidney, which we designate as pure (monotypic) epithelioid patients824 to contrast them from classic metastasis59 that are regarded by some as patients824 . We use the terminology "pure" to separate these cases from those that may have variable epithelioid components. The mean age of the patients was 40.7 years (range, 14 to 68 y). The male-to-female ratio was 1:1. Seventy-nine percent of patients were symptomatic at presentation with metastatic disease at onset in 12 cases. Follow-up and/or disease progression information were available for 33 of 41 cases (mean, 44.5 mo and median, 24.5 mo; range, 4 to 240); 9 patients had a history of associated tuberous sclerosis . Recurrence and metastasis were seen in 17% and 49% of patients ; 33% of patients died of disease. Lymph node involvement was seen in 24% of patients ; the liver (63%), lung (25%), and mesentery (18.8%) were the most common metastatic sites. Clinicopathologic parameters associated with disease progression (recurrence, metastasis , or death due to disease) in univariate analysis included associated tuberous sclerosis complex or concurrent metastasis59 (any metastasis , P=0.046), necrosis ( metastasis at diagnosis, P=0.012), tumor, Tumors, tumors size >7 cm (progression, P=0.021), extrarenal extension and/or renal vein involvement (progression, P=0.023), and patients89 -like growth pattern (progression, P=0.040) (the 5 adverse prognostic parameters for patients824 ). tumor, Tumors, tumors with <2 adverse prognostic parameters (13 cases) were considered to be low risk for progression tumor, Tumors, tumors , with 15% having disease progression. tumor, Tumors, tumors with 2 to 3 adverse prognostic parameters (14 cases) were considered to be "intermediate risk," with 64% having disease progression. tumor, Tumors, tumors with more than 4 or more adverse prognostic parameters (6 cases) were considered to be high risk, with all patients having disease progression. Of tumor, Tumors, tumors with 3 or more adverse prognostic parameters, 80% had disease progression. An exact logistic regression analytic model showed that only patients89 -like growth pattern and extrarenal extension and/or renal vein involvement were significant predictors of outcome (P=0.009 and 0.033, respectively). Our data of a large series with uniform definitional criteria confirm the malignant potential for patients824 and provide adverse prognostic parameters for risk stratification in these patients .
| null |
2266_biomrc
|
Title: Pharmacological modulation of experimental phasic and tonic @entity925 by @entity1286 , @entity680 and XXXX in healthy volunteers.
BACKGROUND: muscle pain, Muscle pain is a major clinical problem but the underlying mechanisms and its pharmacological modulation need further investigation. This study on 15 volunteers evaluates if two experimental muscle pain, Muscle pain models are sensitive to micro -receptor agonists and to an humans024 ( humans43 )-receptor antagonist. METHODS: In the left tibialis anterior, intramuscular electrical (IMES) humans58 thresholds were determined for single (SPTmuscle) and five (RPTmuscle) repeated stimuli. Also humans58 to suprathreshold stimulation at 150% of RPTmuscle, 10 s, was assessed on a visual analog scale (VAS) as AUCimes (area under the VAS curve). In the right TA muscle, humans58 intensity on infusion of 0.5 ml of hypertonic saline, 5% (AUCsaline) and humans58 distribution indicated as local and referred were evaluated. humans58 variables were assessed before, during and after intravenous infusions of humans286 (10 microg x kg-1 humans009 , 10 min), Alfentanil, alfentanil (target-controlled infusion, plasma concentration; 60 ng ml-1, 60 min) and ketamine, Ketamine (10 microg x kg-1 humans009 , 60 min). All data were normalized to baseline humans58 values (before drug infusions were initiated) and compared with placebo ( humans363 , 2 microg x kg-1 humans009 , 10 min). RESULTS: SPTmuscle increased (log mean values +/- SD, mA) with humans286 (0.11 +/- 0.17, P < 0.05), Alfentanil, alfentanil (0.28 +/- 0.24, P < 0.001) and ketamine, Ketamine (0.19 +/- 0.18, P < 0.01) as compared with placebo (-0.03 +/- 0.12). Alfentanil, alfentanil and ketamine, Ketamine also increased RPTmuscle (0.25 +/- 0.21, P < 0.01 and 0.21 +/- 0.19, P < 0.05, respectively) as compared with placebo (0.00 +/- 0.17). humans58 to IMES (AUCimes) was reduced (median values [25th-75th percentiles], cm x s) by Alfentanil, alfentanil and ketamine, Ketamine (-19.7 [-14.6 - -29.6] and-12.8 [-8.3 - -27.8], P < 0.05, respectively) vs. placebo (-0.8 [1.6 - -12.3]). Similar drug effects were seen when humans58 to infusion of hypertonic saline (AUCsaline) was assessed ( Alfentanil, alfentanil :-388 [-99 - -677] and ketamine, Ketamine :-326 [-227 - -573], P < 0.05 compared with placebo: 150 [449--240]). ketamine, Ketamine also reduced the size of the humans58 area (-58.4 [-21.2 - -176.1], < 0.05) as compared with placebo (-0.4 [70.6 - -13.4]). The frequency of humans58 was also lower when ketamine, Ketamine was given (3/13, P < 0.05) vs. placebo (9/14). CONCLUSION: The study demonstrates that experimental muscle pain, Muscle pain induced in humans by electrical stimulation and infusion of hypertonic saline is sensitive to pharmacological modulation similar to preclinical animal tests and clinical trials. The data suggest that these models can be valuable tools in analgesic drug development.
| null |
218_biomrc
|
Title: Improved function of hemiplegic upper extremity after cognitive sensory motor training therapy in @entity64 XXXX : preliminary report of a case series.
BACKGROUND: Recovery of upper extremity functions after a severe stroke, chronic stroke and patients31 ( patients31 ) have generally been less than satisfactory. The "cognitive sensory motor training therapy" is a relatively new method claimed to improve motor control using a specific type of repetitive sensory and motor re-learning protocol. There has been no previous study demonstrating the effectiveness of this method. OBJECTIVE: To investigate the value of the cognitive sensory motor training therapy to improve upper extremity motor function in stroke, chronic stroke and patients31 patients . MATERIAL AND METHOD: Seven patients with persistent impaired upper extremity functions for over 6 months after a stroke, chronic stroke or patients31 were trained with the cognitive sensory motor training therapy program. Hand and arm functions were measured with Action Research Arm (ARA) test before the beginning of the study and once a month thereafter. Data were analyzed retrospectively. RESULTS: There was improvement of ARA scores in all of the trained patients . On average there was an increase of the ARA score of 7.7 points during the average training time of 2.5 months. CONCLUSION: The cognitive sensory motor training therapy may be an effective method for motor rehabilitation of stroke, chronic stroke or traumatic brain injured patients . Further prospective randomized control trials are justified and required.
| null |
220_biomrc
|
Title: Safety of intravenous thrombolysis for acute @entity64 including concomitant XXXX sufferers - experience from Poland.
BACKGROUND: IS, stroke, Stroke, Ischaemic stroke ( IS, stroke, Stroke, Ischaemic stroke ), brain haemorrhage and cerebral venous thrombosis can occur as an early and late complication of neoplastic diseases, neoplastic disease, cancer, Cancer in the clinical course. neoplastic diseases, neoplastic disease, cancer, Cancer patients are at increased risk for IS, stroke, Stroke, Ischaemic stroke from direct and indirect effects of their malignancy. AIMS: The aim of our study was to evaluate the relationship between neoplastic diseases, neoplastic disease, cancer, Cancer and the long-term outcome, mortality and the presence of HT, haemorrhagic complications, bleeding, haemorrhagic transformation in patients with acute IS, stroke, Stroke, Ischaemic stroke treated with i.v. thrombolysis. METHODS: We retrospectively evaluated the demographic and clinical data of 495 Caucasian patients with acute IS, stroke, Stroke, Ischaemic stroke and 40 patients with IS, stroke, Stroke, Ischaemic stroke and concomitant neoplastic diseases, neoplastic disease, cancer, Cancer who were consecutively treated from 2006 to 2013 in two experienced IS, stroke, Stroke, Ischaemic stroke centres. RESULTS: In analysed group, there were 7.8% of patients with neoplastic diseases, neoplastic disease, cancer, Cancer [50.0% male, mean age 72.3 9.3; National Institutes of Health IS, stroke, Stroke, Ischaemic stroke Scale - 13 (range 9.5-17)]. neoplastic diseases, neoplastic disease, cancer, Cancer was diagnosed before i.v.-thrombolysis in 28 (70.0%) patients . After 3 months of follow up, 60% of patients were independent (mRS 0-2) compared with the group of patients without neoplastic diseases, neoplastic disease, cancer, Cancer - 55% (p = 0.54), 17.5% died (18.4%; p = 0.89), 12.4% suffered HT, haemorrhagic complications, bleeding, haemorrhagic transformation ( HT, haemorrhagic complications, bleeding, haemorrhagic transformation ) (17.6%; p = 0.41) and 2.5% experienced SICH (4.4%; p = 0.56, respectively). Other clinical complications were not found. A multivariate analysis showed no impact of neoplastic diseases, neoplastic disease, cancer, Cancer on unfavourable outcomes [modified Rankin scale 3-6)] after 3 months (p = 0.15). CONCLUSION: Intravenous thrombolysis performed in Caucasian IS, stroke, Stroke, Ischaemic stroke patients with past or current neoplastic diseases, neoplastic disease, cancer, Cancer , but not in the course of chemo- and radiotherapy, can be a safe and effective method of treatment. In making decision on the thrombolytic treatment, the risk of HT, haemorrhagic complications, bleeding, haemorrhagic transformation complications and the life expectancy should be assessed.
| null |
2268_biomrc
|
Title: [Etiology and sequelae of perioperative XXXX ].
Accidental hypothermia, hypothermia is a frequent event during the perioperative period. Recent studies revealed a drop in core temperature of over 2 degrees C in more than 50% of all patients undergoing an operation. This drop in core temperature seems to be primarily due to the following factors. Anaesthesia prevents behavioural adaptations to changes in ambient temperature. Simultaneously, autonomic mechanisms of temperature control are suppressed by general as well as by neuraxial anaesthesia. The interthreshold range between core temperatures that trigger responses to warmth and to cold increases up to 20-fold. This is primarily due to a decrease in the cold response threshold. As a result, body core temperature of anaesthetized patients is primarily determined by the much lower temperature of the environment. On one hand, decreases in body temperatures may exert organ protective effects under certain conditions, e.g., by increasing ischemic tolerance. On the other hand, there is accumulating evidence that accidental perioperative Accidental hypothermia, hypothermia may also adversely affect organ function and outcome. For example, unfavourable effects of perioperative Accidental hypothermia, hypothermia on the immune defence, on the function of the coagulation system, on cardiovascular performance, as well as on postoperative recovery have been reported. Consequently, measures should be taken to actively control the perioperative heat balance of patients .
| null |
4312_biomrc
|
Title: Influence of different self-expanding stent-graft types on remodeling of the aortic neck after XXXX repair.
PURPOSE: To evaluate aortic neck changes, specifically infrarenal aortic neck dilatation, aortic neck dilatation , over a 10-year period in patients, men undergoing endovascular aneurysm repair (EVAR) for patients, men028 . METHODS: All patients, men who underwent elective EVAR at our institution from 1998 through 2007 were analyzed retrospectively. Among these, 103 patients, men (96 patients, men ; mean age 71 years, range 35-84) who received the 3 most frequently implanted self-expanding stent-grafts (35 Talent, 39 Excluder, and 29 Zenith) and had a minimum 3-month imaging follow-up at our department were selected for this study. All diameters were measured perpendicular to the centerline of flow on computed tomography; baseline data were derived from the first postoperative scan. Stent-graft migration was measured from the lowermost renal artery to the first strut of the stent-graft. Based on intra- and interobserver error measurements, a minimum change of 2 mm defined infrarenal aortic neck dilatation, aortic neck dilatation . RESULTS: During a mean follow-up of 39.4 months (range 3-108.8), infrarenal aortic neck dilatation, aortic neck dilatation (>2 mm) was found in 10 patients, men (28.6%, 95% CI 14.6-46.3) in the Talent group, 4 (10.3%, 95% CI 2.9-24.2) in the Excluder group, and 9 (31.0%, 95% CI 15.3-50.8) in the Zenith group (p=0.299). In 7 (31%) of the 23 patients, men with neck dilatation, additional therapy was necessary. Suprarenal changes were found in 6 patients, men (17.1%, 95% CI 6.6-33.6), 8 patients, men (20.5%, 95% CI 9.3-36.5), and 5 patients, men (17.2%, 95% CI 5.8-35.8), respectively (p=0.218). Stent-graft migration >2 mm was seen in 2 (1.9%) of the 103 patients, men in follow-up. The overall endoleak rates were 37.1% for Talent, 30.8% for Excluder, and 37.9% for Zenith (p>0.05). Two patients, men were converted to open repair owing to Talent stent-graft migration and persistent type I leak (Zenith). The overall reintervention rate was 13.6% (13/103). CONCLUSION: Only a small number of the patients, men treated with self-expanding stent-grafts show notable infrarenal aortic neck dilatation, aortic neck dilatation , which does not appear to be related to the stent-graft model. Almost all cases of infrarenal aortic neck dilatation, aortic neck dilatation exceeded the percentage of oversizing; less than a third of patients, men with infrarenal aortic neck dilatation, aortic neck dilatation required therapy.
| null |
2271_biomrc
|
Title: A new technique for repair of XXXX caused by @entity397 of the anterior leaflet.
Prolapse of the anterior leaflet of the mitral valve is the result of rupture, ruptured chordae , elongated chordae, or elongated or patients, patient377 muscle. Several techniques have been described for the correction of mitral valve regurgitation, mitral valve insufficiency, mitral insufficiency . However, when there is severe rupture, ruptured chordae of the chordae, the most widely accepted solution is valve replacement. We describe a technique for the creation of a neochorda with a strip of tissue from the anterior leaflet of the mitral valve. This technique was used in two patients, patient with severe mitral valve regurgitation, mitral valve insufficiency, mitral insufficiency . Formation of a neochorda and placement of a Carpentier ring to remodel the anulus obviated the need for a valve replacement. Both patients, patient had an uneventful recovery. Studies performed 3 and 4 months postoperatively showed competent and well-functioning valves. One patients, patient required a valve replacement for acute mitral valve regurgitation, mitral valve insufficiency, mitral insufficiency 5 years later, but the other patients, patient was doing well 3 years after the operation. Despite the limited experience, we believe this technique offers a reasonable alternative to valve replacement.
| null |
226_biomrc
|
Title: Diuretics in the management of XXXX .
Thiazide diuretics have been in use for over 30 years in the treatment of patients, patient01 . Their action results in a reduction in peripheral resistance without a significant decrease in cardiac output or a major shift in plasma volume. They are as or more effective than any of the other antihypertensive agents when used as monotherapy and can serve as baseline therapy in combination with any of the available adrenergic, converting enzyme-inhibiting agents, or calcium -entry blockers. There is a high degree of patients, patient acceptance; titration to an effective dosage is relatively easy; and cost, relatively low. Although certain undesirable metabolic changes may occur following the use of these agents, most of them are controllable, and there is no evidence to date that they offset the benefits achieved by blood pressure lowering. Asymptomatic elevated uric acids have not been shown to be of great significance. If gout occurs, it can be managed. Alterations in glucose metabolism may occur, and in some patients, patient , it appears that blood glucose levels are elevated over time. This is not a desirable metabolic change, but is one of doubtful prognostic significance. Changes in lipids are generally short-term, and in the major clinical trials, lipid levels have not remained elevated with a continuation of diuretic therapy. Although diuretics produce patients, patient371 in a fairly high percentage of patients, patient , this is not generally severe (less than 3.3 mEq per liter) and usually does not produce symptoms. There is no firm evidence that the patients, patient371 produced by diuretics predisposes the patients, patient to severe arrhythmias or sudden death , although this point has been emphasized repeatedly in recent publications. Diuretics can usually be given without potassium -maintenance therapy. However, patients, patient371 should be prevented in the elderly, in patients, patient with ischemic heart disease , left ventricular hypertrophy and those on digitalis, or with diabetes . We prefer potassium -sparing agents along with a diuretic over supplements to prevent patients, patient371 ; the number of pills is kept at a reasonable level, and cost is minimized. Physicians should continue to prescribe diuretics as first-step therapy in the majority of patients, patient to maximize therapeutic outcome.
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2282_biomrc
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Title: Bilateral globus pallidus internus deep brain stimulation for XXXX supports success of cochlear implantation in a 5-year old ex-24 week preterm twin with absent cerebellar hemispheres.
BACKGROUND: Early onset dystonia ( dyskinesia ) and deafness in childhood pose significant challenges for children, child and carers and are the cause of multiple disability . It is particularly tragic when the children, child cannot make use of early cochlear implantation (CI) technology to relieve deafness and improve language and communication, because severe cervical and truncal dystonia brushes off the magnetic amplifier behind the ears. Bilateral globus pallidus internus (GPi) deep brain stimulation (DBS) neuromodulation can reduce dyskinesia , thus supporting CI neuromodulation success. METHODS: We describe the importance of the order of dual neuromodulation surgery for dystonia and deafness . First with bilateral GPi DBS using a rechargeable ACTIVA-RC neurostimulator followed 5 months later by unilateral CI with a Harmony (BTE) Advanced Bionics Hi Res 90 K cochlear device. This double neuromodulation was performed in series in a 12.5 kg 5 year-old ex-24 week gestation-born twin without a cerebellum. RESULTS: Relief of dyskinesia enabled continuous use of the CI amplifier. Language understanding and communication improved. Dystonic storms abated. Tolerance of sitting increased with emergence of manual function. children, child59 ensued 10 days after ACTIVA-RC removal for infection-erosion at 3 years and 10 months. He required intensive care and DBS re-implantation 3 weeks later together with 8 months of hospital care. Today he is virtually back to the level of functioning before the DBS removal in 2012 and background medication continues to be slowly weaned. CONCLUSION: This case illustrates that early neuromodulation with DBS for dystonic cerebral palsy followed by CI for deafness is beneficial. Both should be considered early i.e. under the age of five years. The DBS should precede the CI to maximise dystonia reduction and thus benefits from CI. This requires close working between the paediatric DBS and CI services.
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235_biomrc
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Title: Improving detection of XXXX @entity1489 .
Patients491 ( Patients491 ) and SCCs, squamous cell carcinomas ( SCCs, squamous cell carcinomas ) are clinically and pathologically distinct and both are locally invasive. However, while Patients491 rarely metastasise, SCCs, squamous cell carcinomas have the potential to do so especially when they arise on the ears or lips. UV radiation is the most important risk factor for Patients489 ( Patients489 ). The cancer, tumours most commonly arise in fair-skinned individuals on sun-damaged skin, especially the face. Incidence rises with age. Patients with one Patients489 have a higher risk of developing another Patients489 and of malignant melanoma . SCCs, squamous cell carcinomas are frequently more difficult to diagnose than Patients491 . Well differentiated lesions have a pronounced keratotic element. Poorly differentiated SCCs, squamous cell carcinomas tend to be pink or red papules or nodules, lacking keratin, which may ulcerate. Around 5% of SCCs, squamous cell carcinomas metastasise. High-risk SCCs, squamous cell carcinomas include those: on the ear, lip, or sites unexposed to the sun and in chronic Patients066 , scars or Patients490 . SCCs, squamous cell carcinomas > 20 mm in diameter or > 4 mm in depth are high risk. Patients who are immunosuppressed, have poorly differentiated cancer, tumours or recurrent disease are also at increased risk. Patients with a slowly evolving or persistent skin lesion where cancer, tumours is a possibility should be referred to a dermatologist. Lesions suspected of being BCC should be referred routinely. Urgent referral should be reserved for cases where there is concern that a delay may have a significant impact because of the size or site of the lesion. Any non-healing lesions >1 cm with marked induration on palpation, showing significant expansion over eight weeks, should be referred urgently as they may be SCCs, squamous cell carcinomas .
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236_biomrc
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Title: Mechanical and electromyographic stretch responses in XXXX and healthy subjects.
The reflex mediated mechanical response was studied in the ankle flexors and ankle extensors of healthy and spastic subjects at maintained contractions from low to high concentration levels. This was done by a technique where muscle stretches could be applied during contractions with stretch reflex responses present or during contractions where the stretch reflex was absent. Stretch responses without stretch reflexes were obtained during contractions elicited by electrical stimulation. The validity of this method is discussed in details and it is concluded that the stretch responses during electrical stimulation can give a correct estimate of the non-reflex muscle response. The method is difficult to carry out in many human, patients subjects and a number of precautions have to be taken. In healthy subjects a large reflex mediated mechanical response was found in the ankle flexors and ankle extensors, with the largest response at low and intermediate contraction levels. Surprisingly the reflex mediated mechanical response was found to be of equal size in the ankle extensors of spastic human, patients and control subjects at all contraction levels. In the ankle flexors no reflex mediated mechanical response was present in the human, patients contrary to the findings in the control subjects. A method was developed to predict the reflex mediated mechanical response from the reflex mediated EMG response. The method was successfully applied in the ankle flexors. In the ankle extensors the measured reflex mediated mechanical response was a factor of 2.5 lower than the EMG predicted mechanical reflex response. It was concluded that the method cannot be applied in situations where a large synchronized EMG response occurs--as it does in the ankle extensors. An increased EMG response was found in the ankle extensors in spastic human, patients , but this was not followed by an increased mechanical reflex response. This emphasizes that conclusions drawn from EMG results should be done with caution. Stretch reflexes are increased in spastic human, patients during clinical examination. This is in contrast to the findings under our experimental conditions, where the reflex mediated response during maintained contraction was decreased in the ankle flexors and unchanged in the ankle extensors of spastic human, patients . Others have found that the H-reflex is modulated in healthy subjects in relation to different motor tasks. It was proposed that healthy subjects set the reflex in a facilitated state in relation to ongoing contraction under our experimental conditions and perhaps in a more inhibited state in the clinical test situation.(ABSTRACT TRUNCATED AT 400 WORDS)
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2284_biomrc
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Title: High endothelial venule morphology and function are inducible in germ-free @entity19 : a possible role for XXXX .
Cytokines may facilitate lymphocyte traffic by modulating HEV structure and lymphocyte binding function in accordance with local tissue requirements. This study investigated whether the morphology of HEVs and their lymphocyte binding ligand are altered following antigenic challenge and evaluated the role of IFN-gamma in the induction of such changes. The morphology and lymphocyte binding function of mesenteric LN HEVs of GFM exposed to environmental pathogens were compared to those from GFM and conventional mice . Lymph nodes from all mice had microscopically identifiable HEVs. The morphology of HEVs from GFM was not uniform; many HEVs contained flat endothelial cells with sparse cytoplasm and prominent interendothelial gaps. The number of lymphocytes within the lumen and the HEV wall was low. In contrast, HEVs from GFME and conventional mice were characterized by cuboidal endothelial cells with plentiful cytoplasm and large numbers of lymphocytes in the vessel wall and lumen. There was no delineation of interendothelial cell borders. Lymphocyte binding to HEVs of lymph node sections from GFM was reduced (mean +/- SEM: 1.08 +/- 0.15) compared to that of conventional mice (1.91 +/- 0.20), P less than 0.003. GFME had augmented lymphocyte binding (2.23 +/- 0.26) to levels comparable with those of conventional mice . GFM injected intraperitoneally with IFN-gamma , IFN-alpha beta, or diluent resulted in minor changes in HEV morphology. By contrast, lymphocyte binding to HEV of GFM was more than doubled by the injection of IFN-gamma (1.95 +/- 0.25), P less than 0.01, but not IFN-alpha beta (0.54 +/- 0.07) or the relevant diluent controls (0.89 +/- 0.11, 0.56 +/- 0.06, respectively). It appears that the HEV binding ligand is inducible, and its expression is regulated by at least one immunomodulator, IFN-gamma . Although short-term exposure of HEVs to IFN-gamma influenced HEV function it caused only minor changes in morphology.
| null |
4334_biomrc
|
Title: Macrophages induce @entity3840 expression in @entity0 cells: role of XXXX autoamplification.
tumor, Tumor -associated macrophages and high levels of COX-2, cyclooxygenase-2 ( COX-2, cyclooxygenase-2 ) are associated with poor prognosis in breast cancer human, HCC1954, patients, HCC1937 , but their potential interdependence has not been evaluated. The objective of this study was to determine whether macrophages regulate COX-2, cyclooxygenase-2 expression in breast cancer cells. For this purpose, THP-1 cells were cocultured with human, HCC1954, patients, HCC1937 breast cancer cells. Coculture led to increased COX-2, cyclooxygenase-2 expression in the human, HCC1954, patients, HCC1937 cells and elevated human, HCC1954, patients, HCC1937421 levels in conditioned media. Similar results were observed when THP-1 cells were incubated with human, HCC1954, patients, HCC1937 breast cancer cells or when human, HCC1954, patients, HCC1937 monocyte-derived macrophages were cocultured with human, HCC1954, patients, HCC1937 cells. Coculture triggered production of reactive oxygen species (ROS) in human, HCC1954, patients, HCC1937 cells. COX-2, cyclooxygenase-2 induction was blocked in cells preincubated with an reduced human, HCC1954, patients, HCC1937110 ( human, HCC1954, patients, HCC1937110 ) oxidase inhibitor or by silencing p67PHOX , a subunit of human, HCC1954, patients, HCC1937110 oxidase. ROS production triggered activation of Src and mitogen-activated protein kinases (MAPKs). Blocking Src or MAPK activities or antagonizing the human, HCC1954, patients, HCC19371346 ( human, HCC1954, patients, HCC19371346 ) transcription factor attenuated COX-2, cyclooxygenase-2 induction in human, HCC1954, patients, HCC1937 cells. Coculture caused rapid induction of human, HCC1954, patients, HCC1937356 ( human, HCC1954, patients, HCC1937356 ) in both breast cancer cells and macrophages. Increased human, HCC1954, patients, HCC1937356 expression was blocked by an human, HCC1954, patients, HCC1937193 ( human, HCC1954, patients, HCC1937193 ), suggesting autocrine and paracrine effects. Importantly, macrophage-induced COX-2, cyclooxygenase-2 expression was blocked in human, HCC1954, patients, HCC1937 cells preincubated with human, HCC1954, patients, HCC1937193 or anti- human, HCC1954, patients, HCC1937356 IgG. Together, these results indicate that macrophage-mediated induction of COX-2, cyclooxygenase-2 in breast cancer cells is a consequence of human, HCC1954, patients, HCC1937356 -mediated stimulation of ROS > Src > MAPK > human, HCC1954, patients, HCC19371346 signaling. human, HCC1954, patients, HCC1937356 -dependent induction of COX-2, cyclooxygenase-2 in breast cancer cells provides a mechanism whereby macrophages contribute to tumor, Tumor progression and potential therapeutic targets in breast cancer .
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4336_biomrc
|
Title: [Effects of chronic administration of @entity3371 on vascular reactivity of the XXXX aorta].
The vascular endothelial function is altered in certain vascular pathology such as hypertension, arterial hypertension, hypertensive . However, the endothelial effect of the pharmacologic treatment of this pathology with ACE, angiotensin converting enzyme ( ACE, angiotensin converting enzyme ) inhibitor is poorly understood. To evaluate the effects of long-term treatment of captopril on the rat, rats aortic vascular reactivity, 2 groups of spontaneously hypertension, arterial hypertension, hypertensive rat, rats (SHR) (n = 6) were studied for 12 weeks: the first group was treated with captopril (CAP) (2 g/l of water) while group 2 (OCAP) received no treatment. A third group without hypertension, arterial hypertension, hypertensive was considered as control group (CTL). At the end of the experiments, isolated aortic rings were studied in organ chambers for endothelial and smooth muscle vascular reactivity. The endothelial-dependent relaxations (EDR) to cumulative doses of acetylcholine , histamine or adenosine diphosphate were significantly decreased in the aortic segments of CTL compared to CAP (p < 0.05). Aortic segments from OCAP group demonstrated intermediate EDR responses between CAP and CTL groups. Smooth muscle relaxation to sodium nitroprusside was comparable among the 3 groups. Maximal smooth muscle contraction to progressive doses of norepinephrine was significantly higher in the CTL group compared to the hypertension, arterial hypertension, hypertensive groups. CONCLUSION: Spontaneously hypertension, arterial hypertension, hypertensive rat, rats have an increased basal vascular tone. The increased EDR observed with hypertension, arterial hypertension, hypertensive partially compensates this hypercontractility. Chronic hypertension, arterial hypertension, hypertensive treatment with captopril partially normalizes EDR responses in the rat, rats aorta suggesting the lost of the endothelial compensatory mechanism by ACE, angiotensin converting enzyme inhibitors.
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4337_biomrc
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Title: Perinatal changes in amino acid metabolism of XXXX brain, especially @entity493 and @entity367 .
The changes in both the levels of some free amino acids and their metabolism in the rat, rats brain during the first 24 hr of postnatal life were studied. The content of glutamic acid decreased for the first 2 hr; it remained at the lowest level for the next 4 hr, when it began to increase. The content of alanine, Alanine decreased for the first 6 hr and approached the adult level. Oxygen consumption, glucose oxidation, and pyruvate formation in the cerebral slices of the 24-hr-old rat, rats were as much as 150% of that of the 19-day-old fetus. The distribution profile of radioactivity incorporated into the cerebral amino acids from the subarachnoid-injected [U-14C]glucose was also changed. In the 2- and 6-hr-old rat, rats , 50% of the total radioactivity recovered in the free amino acids was in alanine, Alanine . Its rate decreased to 30% in the 24-hr-old and was 2% in the adult, while the radioactivity incorporated into glutamic acid increased. alanine, Alanine aminotransferase activity started to increase at birth and had the highest level at 24 hr after birth. It then decreased and finally reached the same level as shown at birth. However, aspartate aminotransferase increased during the first 6 hr after birth and did not change until the end of the first day of life.
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2291_biomrc
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Title: Allergen-induced @entity6587 is not a feature of dermal immediate XXXX --in contrast to reactions of airways mucosa.
Allergen challenges of airway mucosa are commonly followed by an increased sensitivity to rechallenge with allergen. In the lower airways this phenomenon has been associated with the late phase of allergic airway reactions, which in turn has been suggested as a link between anaphylaxis and continuous allergic airway disease . The aim of the present investigation was to explore further the phenomenon of allergen-induced hyperreactivity and to see whether it was possible to induce such a reaction in the skin. Twenty-six human, patients with seasonal allergic rhinitis due to birch and/or grass pollens were studied in the pollen-free winter months. Nine of these human, patients had previously demonstrated an increased reactivity following allergen challenge in the nose, and nine of the human, patients had cutaneous allergen-induced late-phase reactions to the allergen tested. Skin-prick tests were performed with pollen allergen, human, patients12 , and a negative control. The areas of the weal-and-flare reactions were measured 15 min after the tests were set. Any late-phase reactions were recorded 6 hr after the skin challenge. The subjects were re-tested with allergen and human, patients12 24 hr after the initial prick test within the area of the corresponding weal from the previous day. In contrast to previous challenges of human, patients airway mucosa, where the same time interval was used, we found no increased responsiveness to rechallenge as compared with the initial allergen challenge. This was true, even if only the subgroups with previously demonstrated nasal allergen-induced hyperreactivity , or cutaneous late-phase reaction were evaluated.(ABSTRACT TRUNCATED AT 250 WORDS)
| null |
2292_biomrc
|
Title: Prevalence of amyloid PET positivity in XXXX syndromes: a meta-analysis.
IMPORTANCE: Amyloid-b positron emission tomography (PET) imaging allows in vivo detection of fibrillar plaques, a core neuropathological feature of participants, patients, participant95 ( participants, patients, participant95 ). Its diagnostic utility is still unclear because amyloid plaques also occur in participants, patients, participant with non- participants, patients, participant95 . OBJECTIVE: To use individual participants, patients, participant data meta-analysis to estimate the prevalence of amyloid positivity on PET in a wide variety of dementias, dementia syndromes. DATA SOURCES: The MEDLINE and Web of Science databases were searched from January 2004 to April 2015 for amyloid PET studies. STUDY SELECTION: Case reports and studies on neurological or participants, patients, participant46 diseases other than dementias, dementia were excluded. Corresponding authors of eligible cohorts were invited to provide individual participants, patients, participant data. DATA EXTRACTION AND SYNTHESIS: Data were provided for 1359 participants, patients, participant with clinically diagnosed participants, patients, participant95 and 538 participants, patients, participant with non- participants, patients, participant95 . The reference groups were 1849 healthy control participants, patients, participant (based on amyloid PET) and an independent sample of 1369 participants, patients, participant95 participants, patients, participant (based on autopsy). MAIN OUTCOMES AND MEASURES: Estimated prevalence of positive amyloid PET scans according to diagnosis, age, and apolipoprotein E, APOE, APOE 4 ( apolipoprotein E, APOE, APOE 4 ) 4 status, using the generalized estimating equations method. RESULTS: The likelihood of amyloid positivity was associated with age and apolipoprotein E, APOE, APOE 4 status. In participants, patients, participant95 , the prevalence of amyloid positivity decreased from age 50 to 90 years in apolipoprotein E, APOE, APOE 4 noncarriers (86% [95% CI, 73%-94%] at 50 years to 68% [95% CI, 57%-77%] at 90 years; n = 377) and to a lesser degree in apolipoprotein E, APOE, APOE 4 carriers (97% [95% CI, 92%-99%] at 50 years to 90% [95% CI, 83%-94%] at 90 years; n = 593; P < .01). Similar associations of age and apolipoprotein E, APOE, APOE 4 with amyloid positivity were observed in participants, patients, participant with participants, patients, participant95 at autopsy. In most non- participants, patients, participant95 dementias, dementia , amyloid positivity increased with both age (from 60 to 80 years) and apolipoprotein E, APOE, APOE 4 carriership ( dementias, dementia with participants, patients, participant832 : carriers [n = 16], 63% [95% CI, 48%-80%] at 60 years to 83% [95% CI, 67%-92%] at 80 years; noncarriers [n = 18], 29% [95% CI, 15%-50%] at 60 years to 54% [95% CI, 30%-77%] at 80 years; frontotemporal dementia : carriers [n = 48], 19% [95% CI, 12%-28%] at 60 years to 43% [95% CI, 35%-50%] at 80 years; noncarriers [n = 160], 5% [95% CI, 3%-8%] at 60 years to 14% [95% CI, 11%-18%] at 80 years; participants, patients, participant294 : carriers [n = 30], 25% [95% CI, 9%-52%] at 60 years to 64% [95% CI, 49%-77%] at 80 years; noncarriers [n = 77], 7% [95% CI, 3%-18%] at 60 years to 29% [95% CI, 17%-43%] at 80 years. CONCLUSIONS AND RELEVANCE: Among participants, patients, participant with dementias, dementia , the prevalence of amyloid positivity was associated with clinical diagnosis, age, and apolipoprotein E, APOE, APOE 4 genotype. These findings indicate the potential clinical utility of amyloid imaging for differential diagnosis in early-onset dementias, dementia and to support the clinical diagnosis of participants, patients, participant with participants, patients, participant95 and noncarrier apolipoprotein E, APOE, APOE 4 status who are older than 70 years.
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244_biomrc
|
Title: Epidemiology of invasive XXXX in lung transplant recipients on long-term azole antifungal prophylaxis.
UNASSIGNED: Lung transplant recipients (LTR) at our institution receive prolonged and mostly lifelong azole antifungal (AF) prophylaxis. The impact of this prophylactic strategy on the epidemiology and outcome of invasive fungal infections, cryptococcal or endemic fungal infections (IFI) is unknown. This was a single-center, retrospective cohort study. We reviewed the medical records of all adult LTR from January 2002 to December 2011. Overall, 16.5% (15 of 91) of patients who underwent lung transplantation during this time period developed IFI. Nineteen IFI episodes were identified (eight proven, 11 probable), 89% (17 of 19) of which developed during AF prophylaxis. LTR with idiopathic pulmonary fibrosis were more likely to develop IFI (HR: 4.29; 95% CI: 1.15-15.91; p = 0.03). A higher hazard of mortality was observed among those who developed IFI, although this was not statistically significant (hazard ratio [HR]: 1.71; 95% confidence interval [CI] [0.58-4.05]; p = 0.27). Aspergillus fumigatus was the most common cause of IFI (45%), with pulmonary parenchyma being the most common site of infection. None of our patients developed disseminated invasive aspergillosis , fungal infections, cryptococcal or endemic fungal infections . IFI continue to occur in LTR, and the eradication of IFI appears to be challenging even with prolonged prophylaxis. Azole resistance is uncommon despite prolonged AF exposure.
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248_biomrc
|
Title: Antitussive activity and other related pharmacological properties of XXXX (AT-17).
Antitussive activity and some other related pharmacological properties of d-3-methyl-N-methylmorphinan were studied. Toxic symptoms in mice and dogs were due to the CNS excitation. Acute toxicity of (AT-17) in mice was slightly (s.c.) or far (p.o.) weaker than that of codeine , but it was three times as toxic as codeine in dogs (i.v.). Antitussive efficacy was about 40% of that of codeine in dogs , whereas 77% as potent as codeine in cats . It showed no relaxing effect on the bronchial muscle of guinea pigs in either normal tone or histamine -induced spasms . It had analgesic effect 1/3 as potent as codeine in mice but it was not antagonized by levallorphan . The prolongation of hexobarbital sleeping time by AT-17 was similar extent to that by codeine . Anti-electroshock effect was half as potent as that of phenobarbital . The inhibitory effect on the transportation of intestinal contents in mice was far weaker than that of codeine . Effect on the respiratory and circulatory systems were also investigated.
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2296_biomrc
|
Title: The behavioral medicine unit: a community hospital model for inpatient treatment of XXXX .
This article describes one community hospital's response to the overwhelming needs of adolescents in central Iowa. It is based on the premise that many youths who have severe depressed, depression do not effectively respond to various outpatient counseling measures, and are in need of some type of inpatient treatment. Most such programs are locked psychiatric units run by patients, child, persons or adolescent psychiatrists. In our case, those wards already in existence are filled to capacity and cannot respond to outside needs. Placing these youth on traditional medical adolescent wards does not work, since medical staff are usually not geared to deal with the many, ever-changing mental health needs of these patients, child, persons . Thus, we developed an unlocked adolescent inpatient unit with a pediatrician experienced in adolescent medicine as medical director; moreover, the program extensively utilizes psychologists, nurse-counselors, social worker-family therapists, recreation therapists, and other specialists. This program is a way for physicians trained in adolescent medicine and other appropriate patients, child, persons to contribute to the complex health needs of youth; it is preferable to do this rather than send all depressed, depression teenagers away by referrals, as seems to happen in some cases. It is also an important way for physicians and other specialists to demonstrate their expertise--both the Society for Adolescent Medicine and American Academy of Pediatrics have advocated such a demonstration--and to give physicians important training in the medical and mental health care needs of youth. Finally, the Spectrum Unit program provides a meaningful way for the primary care physician to be involved in the inpatient treatment of depressed, depression adolescent patients, child, persons .(ABSTRACT TRUNCATED AT 250 WORDS)
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255_biomrc
|
Title: Myocardial vacuolization, a marker of XXXX , in surveillance cardiac biopsies posttransplant: Correlations with morphologic vascular disease and @entity409 .
Allograft vasculopathy (AV) causes intimal thickening with progressive luminal obstruction , endothelial dysfunction, Endothelial dysfunction , and abnormal vasomotion. Subendocardial vacuolization indicating ongoing ischemic injury, ischemia was observed at autopsy in transplanted hearts with severe AV. Whether myocyte vacuolization can be observed with lesser degrees of AV in cardia transplant patients has not been reported. Thirty-nine cardiac transplant patients without flow-limiting disease in large epicardial arteries underwent invasive assessment of AV. Eight to 10 segments of the left anterior descending artery were analyzed by intracoronary ultrasound, and an average intimal index was calculated. Endothelial response to acetylcholine was assessed with serial quantitative angiography. Endomyocardial biopsies taken 5 to 7 days prior to the invasive studies were histopathologically reviewed for the presence of small intramyocardial arteries and myocyte vacuolization. Myocyte vacuolization was evident in biopsies from 20 patients (51%). Intramyocardial arteries were observed in 30 cases (76%); 14 had abnormal arteries. All patients had some degree of intimal thickening by intracoronary ultrasound, and 7 (17 %) had severely abnormal average intimal index (>0.2). endothelial dysfunction, Endothelial dysfunction was present in 23 patients (58%). Vacuolization failed to show an association with abnormal small artery histology or large epicardial artery ultrasound disease. However, a significant association between vacuolization and endothelial dysfunction, Endothelial dysfunction was observed (p = 0.05). Myocyte vacuolization, possibly indicating ischemic injury, ischemia , is common in biopsies from cardiac transplant patients and is associated with abnormal acetylcholine response in large epicardial arteries. We speculate that myocyte vacuolization may be caused at least in part by impaired coronary flow associated with endothelial dysfunction, Endothelial dysfunction .
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259_biomrc
|
Title: Comorbid XXXX among @entity1 with @entity308 .
PURPOSE: To investigate the patients314 ( patients314 ) diagnosed in patients with depressive disorders, depressive disorder, depression, major depressive disorder . MATERIAL AND METHODS: This study included a cross-sectional analysis, and was an extension of the Thai Study of patients316 (THAISAD) project. Eighty-five outpatients with depressive disorders, depressive disorder, depression, major depressive disorder were interviewed using the Mini International Neuropsychiatric Inventory to assess for depressive disorders, depressive disorder, depression, major depressive disorder , in accordance with the Diagnostic and Statistical Manual of patients46 , Fourth Edition, Text Revision and using the Thai version of the Structured Clinical Interview for patients314 to assess for patients726 . RESULTS: Seventy-seven percent of the patients had at least one patients726 , 40% had one patients726 and 60% had two or more patients314 (mixed cluster). The most common patients314 found were borderline patients726 (20%) and obsessive-compulsive PD (10.6%), while the occurrence of avoidant patients726 was low when compared to the findings of previous, related studies. Among the mixed cluster, cluster A combined with cluster C was the common mix. Both patients4256 and double depressive disorders, depressive disorder, depression, major depressive disorder were found to have a higher proportion of patients314 than depressive disorders, depressive disorder, depression, major depressive disorder (85.7% versus 76.1%). Dependent patients726 was found to be less common in this study than in previous studies, including those carried out in Asia. CONCLUSION: The prevalence of patients314 among those with depressive disorders, depressive disorder, depression, major depressive disorder varied, and only borderline patients726 seems to be consistently high within and across cultures. Mixed cluster plays a prominent role in depressive disorders, depressive disorder, depression, major depressive disorder , so more attention should be paid to patients in this category.
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2308_biomrc
|
Title: Pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential XXXX variety.
BACKGROUND: Pyramiding multiple genes into a desirable genetic background can take years to accomplish. In this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. PinK3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (Sub) and susceptible to BB, bacterial leaf blight ( BB, bacterial leaf blight ), leaf-neck blast (BL) and the BPH, brown planthopper ( BPH, brown planthopper )-was used as a genetic basis for significant improvements through gene pyramiding. RESULTS: Four resistance donors with five target genes (Sub1A-C, xa5, Xa21, TPS and SSIIa) and three QTLs (qBph3, qBL1 and qBL11) were backcrossed individually using markers into the pseudo-recurrent parent 'PinK3' via one cycle of backcrossing followed by two cycles of pseudo-backcrossing and three selfings with rigorous foreground marker-assisted selection. In total, 29 pseudo-backcross inbred lines (BILs) were developed. Genome composition was surveyed using 61 simple sequence repeats (SSRs), 35 of which were located on six carrier chromosomes, with the remainder located on six non-carrier chromosomes. The recurrent genome content (%RGC) and donor genome content (%DGC), which were based on the physical positions of BC1F2, ranged from 69.99 to 88.98% and 11.02 to 30.01%, respectively. For the pseudo-BC3F3BILs, the %RGC and %DGC ranged from 74.50 to 81.30% and 18.70 to 25.50%, respectively. These results indicated that without direct background selection, no further increases in %RGC were obtained during pseudo-backcrossing, whereas rigorous foreground marker-assisted selection tended to reduce linkage drag during pseudo-backcrossing. The evaluation of new traits in selected pseudo-BC3F3BILs indicated significant improvements in resistance to BB, bacterial leaf blight , BL, BPH, brown planthopper and Sub compared with PinK3, as well as significant improvements in grain yield (21-68%) over the donors, although yield was 7-26% lower than in 'PinK3'. All pyramided lines were aromatic and exhibited improved starch profiles, rendering them suitable for industrial food applications. CONCLUSIONS: Results show that our new pyramiding platform, which is based on marker-assisted pseudo-backcrossing, can fix five target genes and three QTLs into a high-yielding pseudo-recurrent background within seven breeding cycles in four years. This multiple pseudo-backcrossing platform decreases the time required to generate new rice varieties exhibiting complex, durable resistance to biotic and abiotic stresses in backgrounds with desirable qualities.
| null |
4357_biomrc
|
Title: EEG spectral power in phasic and tonic REM sleep: different patterns in young adults and XXXX .
UNASSIGNED: Rapid eye movement sleep is composed of phasic and tonic periods, two distinguishable microstates in terms of arousal thresholds and sensory processing. Background electroencephalogram oscillations are also different between periods with (phasic state) and periods without (tonic state) eye movements. In Study 1, previous findings analysing electroencephalogram spectral power in phasic and tonic rapid eye movement sleep, rapid eye movement sleep were replicated, and analyses extended to the high gamma range (52-90 Hz). In Study 2, phasic and tonic spectral power differences within a group of 4-8-year-old children were examined. Based on the polysomnographic data of 20 young adults, the phasic state yielded increased delta and theta power in anterior sites, as well as generally decreased high alpha and beta power in comparison to the tonic state. Moreover, phasic periods exhibited greater spectral power in the lower and the higher gamma band. Interestingly, children (n = 18) exhibited a different pattern, showing increased activity in the low alpha range during phasic periods. Moreover, during phasic in contrast to tonic rapid eye movement sleep, rapid eye movement sleep , increased low and high gamma and enhanced low gamma band power emerged in anterior and posterior regions, respectively. The current findings show that spectral activity within the high gamma range substantially contributes to the differences between phasic and tonic rapid eye movement sleep, rapid eye movement sleep , especially in adults. Moreover, the current data underscore the heterogeneity of tonic rapid eye movement sleep, rapid eye movement sleep , and point to marked differences between young adults and children regarding phasic/tonic electroencephalogram spectral power. These results suggest that the differentiation between phasic and tonic rapid eye movement periods undergoes maturation.
| null |
262_biomrc
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Title: Immunotherapy in spring-time hay XXXX . A clinical and immunological study comparing two different treatment extract compositions.
In a study of the efficacy of two different treatment schedules for perennial immunotherapy, 47 adult Patients, patients, patient with spring-time hay fever due to allergy against birch and other deciduous trees were randomly assigned to three treatment groups: one group received birch, alder and hazel allergen in Allpyral, another group received the same Allpyral mixture and in addition all relevant tree pollens in aqueous extract and a control group received no injections. For determination of antibody titres the radioallergosorbent test (RAST) and the ammonium sulphate precipitation (ASP) technique were used. Cellular responsiveness was studied by measuring birch pollen (BP) induced leucocyte Patients, patients, patient12 release in peripheral blood. The clinical and immunological response was similar in the two treated groups. Treated Patients, patients, patient had less symptoms and a lower consumption of Patients, patients, patient6206 tablets during the pollen season than the control group. Non-IgE BP antibodies and IgE antibodies recorded with the ASP technique increased after immunotherapy while RAST values did not change significantly. A decrease of RAST values from postseasonal values during the first year to preseasonal values in the following year was seen in all Patients, patients, patient groups but was less pronounced in treated than in untreated Patients, patients, patient . The decrease was more pronounced in Patients, patients, patient with high RAST values of postseasonal sera than in Patients, patients, patient with low RAST values. Cellular reactivity increased slightly during the first phase of therapy but returned to the pre-treatment level later. Clinical improvement was positively correlated to the percentage increase of non-IgE antibody titre and to the pre-treatment non-IgE/IgE antibody ratio. Patients, patients, patient with high preseasonal RAST titres or high cellular sensitivity tended to have more severe symptoms during the pollen season. It is concluded that a mixture of birch, alder and hazel is sufficient for immunotherapy in spring-term hay fever . It is obvious that changes of a single immunological variable do not account for the therapeutic results in immunotherapy.
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4359_biomrc
|
Title: Combination therapy of renin-angiotensin system inhibitors plus @entity460 channel blockers versus other two-drug combinations for XXXX : a systematic review and meta-analysis.
UNASSIGNED: Many randomized clinical trials (RCTs) have investigated the efficacy and safety of renin-angiotensin system inhibitors (RASIs) plus calcium channel blockers (CCBs), compared with other two-drug combinations, but systematic assessment in this aspect is still lacking. We carried out the present meta-analysis of randomized controlled trials to evaluate the long-term effect and safety of RASIs plus CCBs. Literatures were searched in MEDLINE, EMBASE and Cochrane Central Register of Controlled Trials in September 2014. A fixed-effect model was used to estimate the pooled effect of trials identified. Thirty-four trials with 41 694 patients, Human were included. Compared with RASIs plus diuretics, RASIs plus CCBs decreased total cardiovascular (CV) events (relative risk (RR) 0.82, 95% confidence interval (CI): 0.75, 0.91, adjusted RR (ARR) 1.7%) and withdrawals due to adverse effect (WDAE) (RR 0.87, 95% CI: 0.80, 0.94, ARR 1 .3%). Compared with CCBs plus diuretics, RASIs plus CCBs decreased WDAE (RR 0.63, 95% CI: 0.45, 0.90, ARR 1 .1%). Our meta-analysis indicates that RASIs plus CCBs provide a superior safety and prevention of CV events to RASIs plus diuretics, whereas this combination is also safer than CCBs plus diuretics. We also raise a new hypothesis. More high-quality RCTs focused on hard end points with CV, cerebrovascular and renal events are needed to confirm the hypothesis we have brought out.Journal of patients, Human patients, Human01 advance online publication, 7 January 2016; doi:10.1038/jhh.2015.125.
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260_biomrc
|
Title: Frequency of Cardiovascular Involvement in @entity305 in Brazilian XXXX .
BACKGROUND: FAP, Familial amyloidotic polyneuropathy ( FAP, Familial amyloidotic polyneuropathy ) is a rare disease diagnosed in Brazil and worldwide. The frequency of cardiovascular involvement in Brazilian FAP, Familial amyloidotic polyneuropathy Patients, patients, patient is unknown. OBJECTIVE: Detect the frequency of cardiovascular involvement and correlate the cardiovascular findings with the modified PND, polyneuropathy disability ( PND, polyneuropathy disability ) score. METHODS: In a national reference center, 51 Patients, patients, patient were evaluated with clinical examination, electrocardiography (ECG), echocardiography (ECHO), and 24-hour Holter. Patients, patients, patient were classified according to the modified PND, polyneuropathy disability score and divided into groups: PND, polyneuropathy disability 0, PND, polyneuropathy disability I, PND, polyneuropathy disability II, and PND, polyneuropathy disability > II (which included PND, polyneuropathy disability IIIa, IIIb, and IV). We chose the classification tree as the statistical method to analyze the association between findings in cardiac tests with the neurological classification ( PND, polyneuropathy disability ). RESULTS: ECG abnormalities were present in almost 2/3 of the FAP, Familial amyloidotic polyneuropathy Patients, patients, patient , whereas ECHO abnormalities occurred in around 1/3 of them. All Patients, patients, patient with abnormal ECHO also had abnormal ECG, but the opposite did not apply. The classification tree identified ECG and ECHO as relevant variables (p < 0.001 and p = 0.08, respectively). The probability of a Patients, patients, patient to be allocated to the PND, polyneuropathy disability 0 group when having a normal ECG was over 80%. When both ECG and ECHO were abnormal, this probability was null. CONCLUSIONS: Brazilian Patients, patients, patient with FAP, Familial amyloidotic polyneuropathy have frequent ECG abnormalities. ECG is an appropriate test to discriminate asymptomatic carriers of the mutation from those who develop the disease, whereas ECHO contributes to this discrimination.
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2312_biomrc
|
Title: In-hospital outcome in octogenarians with XXXX undergoing emergent coronary angiography.
Very elderly patients have higher mortality rates than younger patients after patients187 ( patients187 ). However, the mechanism by which increasing age contributes to such mortality remains unclear. In addition, the efficacy and safety of invasive coronary procedures for octogenarians with patients187 have not been well established. We compared the clinical characteristics and in-hospital outcome of 193 octogenarians (mean age, 83 years) with those of 1,462 younger patients (mean age, 64 years) with patients187 who underwent emergent coronary angiography. Octogenarians included a greater number of females, had higher rates of cerebrovascular disease and multivessel disease , a higher Killip class, a higher Forrester class, and lower rates of smoking, diabetes , and patients007 than the younger subjects. Interventions, including percutaneous transluminal coronary angioplasty (PTCA) and coronary artery bypass grafting (CABG), were performed less frequently in octogenarians than in younger patients (88.0% versus 90.8%). The procedural success rate in octogenarians did not differ from that in younger patients . However, the in-hospital mortality rate for the octogenarians was about three times higher than for the younger patients (19.2% versus 6.9%). Multivariate analysis revealed that the predictors of in-hospital mortality in the octogenarians were a higher Killip class and a higher Forrester class. Octogenarians with patients187 had fewer coronary risk factors and a similar success rate for the intervention, but had more greatly impaired hemodynamics and higher in-hospital mortality than the younger patients . Therefore, impaired myocardial reserve may contribute to a large portion of in-hospital deaths in octogenarians with patients187 .
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4364_biomrc
|
Title: Photoinduced Dynamics and XXXX of a Cancer Drug in Proximity of Inorganic Nanoparticles under Visible Light.
UNASSIGNED: Drug sensitization with various inorganic nanoparticles (NPs) has proved to be a promising and an emergent concept in the field of nanomedicine. Rose bengal (RB), a notable photosensitizer, triggers the formation of reactive oxygen species under green-light irradiation, and consequently, it induces toxicity, cytotoxicity and cell death. In the present study, the effect of photoinduced dynamics of RB upon complexation with semiconductor zinc oxide NPs is explored. To accomplish this, we successfully synthesized nanohybrids of RB with ZnO NPs with a particle size of 24 nm and optically characterized them. The uniform size and integrity of the particles were confirmed by high-resolution transmission electron microscopy. UV/Vis absorption and steady-state fluorescence studies reveal the formation of the nanohybrids. ultrafast picosecond-resolved fluorescence studies of RB- ZnO nanohybrids demonstrate an efficient electron transfer from the photoexcited drug to the semiconductor NPs. Picosecond-resolved F rster resonance energy transfer from ZnO NPs to RB unravel the proximity of the drug to the semiconductor at the molecular level. The photoinduced ROS formation was monitored using a dichlorofluorescin oxidation assay, which is a conventional oxidative stress indicator. It is observed that the ROS generation under green light illumination is greater at low concentrations of RB- ZnO nanohybrids compared with free RB. Substantial photodynamic activity of the nanohybrids in bacterial and fungal cell lines validated the in vitro toxicity, cytotoxicity results. Furthermore, the cytotoxic effect of the nanohybrids in HeLa cells, which was monitored by MTT assay, is also noteworthy.
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269_biomrc
|
Title: Phase II study of @entity782 for treatment of relapsed or refractory indolent non-Hodgkin's lymphoma and XXXX .
PURPOSE: We performed a phase II study of oral vorinostat , a histone and protein deacetylase inhibitor, to examine its efficacy and tolerability in patients, patient, PATIENTS with relapsed/refractory indolent lymphoma, refractory follicular lymphoma . patients, patient, PATIENTS AND METHODS: In this open label phase II study (NCT00253630), patients, patient, PATIENTS with relapsed/ indolent lymphoma, refractory follicular lymphoma (FL), patients, patient, PATIENTS074 ( patients, patient, PATIENTS074 ), or patients, patient, PATIENTS073 ( patients, patient, PATIENTS073 ), with <= 4 prior therapies were eligible. Oral vorinostat was administered at a dose of 200 mg twice daily on days 1 through 14 of a 21-day cycle until progression or unacceptable patients, patient, PATIENTS37 . The primary end point was objective response rate (ORR), with secondary end points of progression-free survival (PFS), time to progression, duration of response, safety, and tolerability. RESULTS: All 35 eligible patients, patient, PATIENTS were evaluable for response. The median number of vorinostat cycles received was nine. ORR was 29% (five complete responses [CR] and five partial responses [PR]). For 17 patients, patient, PATIENTS with FL, ORR was 47% (four CR, four PR). There were two of nine responders with patients, patient, PATIENTS074 (one CR, one PR), and no formal responders among the nine patients, patient, PATIENTS with patients, patient, PATIENTS073 , although one patients, patient, PATIENTS maintained stable disease for 26 months. Median PFS was 15.6 months for patients, patient, PATIENTS with FL, 5.9 months for patients, patient, PATIENTS073 , and 18.8 months for patients, patient, PATIENTS074 . The drug was well-tolerated over long periods of treatment, with the most common grade 3 adverse events being thrombocytopenia , anemia , patients, patient, PATIENTS210 , and patients, patient, PATIENTS295 . CONCLUSION: Oral vorinostat is a promising agent in FL and patients, patient, PATIENTS074 , with an acceptable safety profile. Further studies in combination with other active agents in this setting are warranted.
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270_biomrc
|
Title: A novel soy-based meal replacement formula for XXXX among @entity28 individuals: a randomized controlled clinical trial.
OBJECTIVE: To assess the efficacy and safety of a low calorie soy-based meal replacement program for the treatment of hundred obese, obesity . DESIGN: A 12-week prospective randomized controlled clinical trial. SETTING: Outpatient weight control research unit. SUBJECTS: One hundred obese, obesity (28<BMI<or=41 kg/m(2)) volunteers between the ages of 35 and 65 y. Seventy-four participants, Participants completed the trial. INTERVENTION: participants, Participants were randomized to either the meal replacement treatment group (n=50; 240 g/day, 1200 kcal/day) or control group (n=50). Both groups at baseline received a single dietary counseling session and a pamphlet describing weight loss practices. MAIN OUTCOME MEASURES: Weight, body fat, serum lipid concentrations. RESULTS: : By intent-to-treat analysis, the treatment group lost significantly more weight than the control group (7.00 vs 2.90 kg; P<0.001) and had a greater change in total (22.5 vs 6.8 mg/dl; P=0.013) and LDL participants, Participants65 (21.2 vs 7.1 mg/dl; P<0.009). Among completers only, the treatment group again lost more weight (7.1 kg; n=37 vs 2.9 kg; n=37; P=0.0001) and had a greater reduction in total participants, Participants65 (26.1 mg/dl; n=37 vs 6.7 mg/dl; P=0.0012) and a greater change in LDL participants, Participants65 (21.6 vs 5.5 mg/dl; P=0.0025). (For any given degree of weight loss , the reduction in LDL participants, Participants65 was significantly greater in the treatment group.) Treatment was well tolerated and no serious side effects were detected. CONCLUSIONS: Use of this soy-based meal replacement formula was effective in lowering body weight, fat mass and in reducing LDL participants, Participants65 beyond what could be expected given the weight lost.
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4368_biomrc
|
Title: [Treatment of XXXX through trans-fibular anterior-lateral approach].
OBJECTIVE: To explore the methods and therapeutic effects of trans-fibular anterior-lateral approach combined with external fixation in the treatment of patients74 . METHODS: From 2007 to 2010,9 patients including 7 males and 2 females with the mean age of 40 years(ranging from 29 to 51 years). All patients received internal fixation of fibula after debridement on the first phase, external fixator were used to fix tibia across ankle joint, and removed after successful skin graft; The second phase tibia was used to fix through the lateral incision used in phase I. Early functional exercise was encouraged ,the union condition and functional results of the ankle joint was evealuated. The criteria of the AOFAS Foot and Ankle Surgery was used to evaluate the effects. RESULTS: All patients were followed up,and the duration ranged for 8 to 37 months(averaged 21 months). Nine patients were achieved bony union, the average healing time was 24 weeks. No plate rupture or screw loosening was found. According to the AOFAS Foot and Ankle Surgery evaluation system, 3 cases got excellent results, 4 good cases and 2 fair. CONCLUSION: Trans-fibular anterior-lateral approach combined with external fixation for patients74 can receive satisfactory reset, debond ankle joint eralier and imporove the clinical effects.
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2323_biomrc
|
Title: Comparison of @entity2247 , Ki-67, and @entity588 expression in normal esophageal mucosa, XXXX , dysplasia, and @entity420 with postablation mucosa and implications for ablative therapies.
BACKGROUND: The neosquamous mucosa that replaces ablated esophageal endothelium after endoscopic mucosal ablation for "Barretts esophagus", "Barretts metaplasia" or patients893 ( patients893 ) may retain buried glandular tissue. This study aimed to assess the neoplastic potential, cellular proliferation, and resistance to apoptosis of this buried glandular tissue by measuring COX-2 , Ki-67, and BCL-2 expression in these tissues. METHODS: A prospectively collected database was sourced for esophageal biopsy specimens with normal histologic appearance, "Barretts esophagus", "Barretts metaplasia" , patients893 , adenocarcinoma , and postablation mucosa comprising ablated Barrett's and ablated patients893 . Quantitative analysis of cellular markers was achieved immunohistochemically using monoclonal antibodies for the COX-2 enzyme (suggesting increased neoplastic potential), Ki-67 antigen (suggesting cellular proliferation), and BCL-2 oncoprotein (suggesting oncogenic resistance to apoptosis). Grading was performed by independent, blinded observers, and the pre- and postablation cellular disparities were subsequently noted. RESULTS: The buried glandular elements of postablation mucosa demonstrated universally greater COX-2 , Ki-67, and BCL-2 expression than normal esophagus. "Barretts esophagus", "Barretts metaplasia" and adenocarcinoma expressed significantly greater COX-2 and Ki-67 at the deep glandular level than postablation mucosa. patients893 demonstrated greater Ki-67 expression than the postablation tissue but only within the superficial glands. Overall, the expression of COX-2 correlated significantly with Ki-67 expression in deep glandular tissue. CONCLUSIONS: Ablation of pathologic mucosa in "Barretts esophagus", "Barretts metaplasia" and patients893 reduces the expression of some markers of patients46 . However, the buried glandular tissue of the postablation mucosa still exhibits a higher expression than normal esophageal epithelium. This has potential implications for the follow-up treatment of these patients because it is unclear whether the true risk of neoplastic progression is adequately reduced. A more comprehensive study is required to address this issue.
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2324_biomrc
|
Title: Quantification in MALDI- XXXX imaging: what can we learn from MALDI-selected reaction monitoring and what can we expect for imaging?
UNASSIGNED: Quantification by mass spectrometry imaging (Q- MSI ) is one of the hottest topics of the current discussions among the experts of the MS imaging community. If MSI is established as a powerful qualitative tool in drug and biomarker discovery, its reliability for absolute and accurate quantification (QUAN) is still controversial. Indeed, Q- MSI has to deal with several fundamental aspects that are difficult to control, and to account for absolute quantification. The first objective of this manuscript is to review the state-of-the-art of Q- MSI and the current strategies developed for absolute quantification by direct surface sampling from tissue sections. This includes comments on the quest for the perfect matrix-matched standards and signal normalization approaches. Furthermore, this work investigates quantification at a pixel level to determine how many pixels must be considered for accurate quantification by ultraviolet matrix-assisted laser desorption/ionization (MALDI), the most widely used technique for MSI . Particularly, this study focuses on the MALDI-selected reaction monitoring (SRM) in rastering mode, previously demonstrated as a quantitative and robust approach for small analyte and peptide-targeted analyses. The importance of designing experiments of good quality and the use of a labeled compound for signal normalization is emphasized to minimize the signal variability. This is exemplified by measuring the signal for cocaine and a tryptic peptide (i.e., obtained after digestion of a monoclonal antibody) upon different experimental conditions, such as sample stage velocity, laser power and frequency, or distance between two raster lines. Our findings show that accurate quantification cannot be performed on a single pixel but requires averaging of at least 4-5 pixels. The present work demonstrates that MALDI-SRM/ MSI is quantitative with precision better than 10-15 %, which meets the requirements of most guidelines (i.e., in bioanalysis or toxicology) for quantification of drugs or peptides from tissue homogenates.
| null |
4374_biomrc
|
Title: Pharmacogenomics of XXXX : a genome wide, placebo controlled cross over study, using four classes of antihypertensive drugs.
BACKGROUND: Identification of genetic markers of antihypertensive drug responses could assist in individualization of patients01 treatment. METHODS AND RESULTS: We conducted a genome-wide association study to identify gene loci influencing the responsiveness of 228 male patients to 4 classes of antihypertensive drugs. The Genetics of Drug Responsiveness in Essential patients01 (GENRES) study is a double-blind, placebo-controlled cross-over study where each subject received amlodipine , bisoprolol , hydrochlorothiazide , and losartan , each as a monotherapy, in a randomized order. Replication analyses were performed in 4 studies with patients of European ancestry (PEAR Study, N=386; GERA I and II Studies, N=196 and N=198; SOPHIA Study, N=372). We identified 3 single-nucleotide polymorphisms within the ACY3 gene that showed associations with bisoprolol response reaching genome-wide significance (P<5x10(-8))however, this could not be replicated in the PEAR Study using atenolol . In addition, 39 single-nucleotide polymorphisms showed P values of 10(-5) to 10(-7). The 20 top-associated single-nucleotide polymorphisms were different for each antihypertensive drug. None of these top single-nucleotide polymorphisms co-localized with the panel of >40 genes identified in genome-wide association studies of patients01 . Replication analyses of GENRES results provided suggestive evidence for a missense variant ( rs3814995 ) in the nephrin, NPHS1 ( nephrin, NPHS1 ) gene influencing losartan response, and for 2 variants influencing hydrochlorothiazide response, located within or close to the patients7610 ( rs3825926 ) and CLIC5 ( rs321329 ) genes. CONCLUSIONS: These data provide some evidence for a link between biology of the glomerular protein nephrin, NPHS1 and antihypertensive action of angiotensin receptor antagonists and encourage additional studies on aldehyde dehydrogenase mediated reactions in antihypertensive drug action .
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4375_biomrc
|
Title: Overall survival after pelvic exenteration for gynecologic XXXX .
BACKGROUND: Five-Year survival after pelvic exenteration for gynecologic malignancy, malignancies has been reported as high as 60%. The objective of this study was to determine women, patients, patient349 ( women, patients, patient349 ) after pelvic exenteration and evaluate factors impacting outcome. METHODS: A retrospective review of all women, patients, patient who underwent pelvic exenteration at our institution between February 1993 and December 2010 was performed. women, patients, patient349 was defined as time from exenteration to date of death or last contact. Survival analysis was performed using the Kaplan Meyer method. Multivariate analysis was performed to determine the impact of clinical and pathologic factors on survival outcomes. RESULTS: One hundred sixty women, patients, patient with gynecologic malignancy, malignancies underwent pelvic exenteration. Five-year women, patients, patient349 ( women, patients, patient349 ) was 33% (95%CI 0.25-0.40). Factors which negatively impacted women, patients, patient349 included shorter treatment-free interval (p=.050), vulvar primary (p=.032), positive margins (p<.001), lymphovascular space invasion (LVSI, p<.001), positive lymph nodes (p<.001) and perineural invasion (p=0.030). In multivariate analysis, positive margins (p=.040), positive nodes (p<.001) and lymphovascular space invasion (LVSI, p=.003) retained a significant impact on women, patients, patient349 . Five-year women, patients, patient349 was 40% (95% CI 0.32-0.48). Factors which negatively impacted women, patients, patient349 included vulvar primary (p=.04), positive margins (p<.001), LVSI (p<.001), positive lymph nodes (p<.001) and perineural invasion (p=.008). In multivariate analysis, positive nodes (p=.001) and LVSI (p=.001) retained a significant impact on women, patients, patient349 . CONCLUSION: Five-year women, patients, patient349 after pelvic exenteration was 40%. Survival outcomes have not significantly improved despite improvements in technique and women, patients, patient selection. Multiple non-modifiable factors at the time of exenteration are associated with poor survival.
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